FastQCFastQC Report
Thu 31 Jul 2014
20S15B_CTTGTA_L003_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20S15B_CTTGTA_L003_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12096099
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 134434 1.1113830996257554 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 129202 1.0681294853820227 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 119194 0.9853920673102957 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 81395 0.6729028920811577 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 79628 0.6582948767201723 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 64810 0.5357925724648913 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 62033 0.5128347577181701 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 61205 0.5059895756474877 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 58143 0.4806756293909301 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 49629 0.4102893007076083 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 45784 0.3785021931450792 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 45529 0.37639407547838355 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 44977 0.3718306207645953 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 42371 0.35028648492377584 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 41169 0.3403493969419397 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 40558 0.3352981816699747 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 36372 0.3006919834237468 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 35990 0.2975339404877556 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 34963 0.28904359992424006 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 34672 0.28663786564577554 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 34024 0.2812807666339371 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 33423 0.276312222643019 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 30651 0.25339574353682126 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 28610 0.2365225350751511 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 26986 0.22309671903313621 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 26983 0.22307191764882214 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26263 0.2171195854134461 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 25844 0.2136556587375814 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 24471 0.2023048918498435 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 23488 0.19417830492293425 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 21519 0.17790032968480168 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21274 0.17587488329915288 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21241 0.17560206807169815 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 20018 0.16549137039966355 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 19109 0.1579765509525013 No Hit
GCTGCTGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 18180 0.15029638894324526 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 17346 0.14340160410393465 No Hit
TGACGAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 16659 0.13772208709601333 No Hit
TGGCTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 16405 0.13562223655742234 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 16282 0.1346053798005456 No Hit
GCAGATGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 15895 0.13140600122403098 No Hit
GCAGTAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 15822 0.13080250087238868 No Hit
GCAGGAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 15813 0.1307280967194465 No Hit
TTGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 15742 0.13014113062401358 No Hit
TGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 15708 0.12986004826845415 No Hit
AGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15693 0.12973604134688382 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 15323 0.1266772039481489 No Hit
TAGATGAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 15089 0.12474269597165168 No Hit
GGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 14891 0.12310580460692327 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 14133 0.11683932150356904 No Hit
CTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGA 13768 0.11382181974535757 No Hit
GCTGTGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 13260 0.10962211866817559 No Hit
CTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCA 13019 0.10762974079494554 No Hit
GACCACACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 12999 0.10746439823285177 No Hit
ACAGGGGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 12972 0.10724118577402515 No Hit
GTGTCAAACTCAAATTTAATTTCTTTCCAAACTTCACAAGCAGCAGCTAA 12770 0.105571225896878 No Hit
GCTGCCGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 12543 0.10369458781711359 No Hit
GCAGCAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 12151 0.10045387360007553 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 8841810 5.931138 10.960142 7
AGCAG 5574160 5.221924 58.68129 20-24
TAGCA 6132185 4.6063714 37.396954 20-24
CACGG 3574990 4.558623 43.99354 45-49
ACTTG 4948780 4.2703614 98.501564 8
GCAGA 4494350 4.2103486 37.407845 25-29
CCTGC 2914490 4.056222 49.25322 15-19
GTTTC 4084995 4.049308 39.346306 40-44
TCACG 3781285 3.8662581 35.39023 45-49
CTGCG 2543860 3.726274 50.716534 15-19
CGGAT 3385665 3.6434917 34.0465 45-49
GAAGT 4598385 3.6355612 33.74327 7
GAACC 3974380 3.5375133 30.637117 70-74
AGAGA 5051635 3.4767652 21.807117 90-94
CTTGA 3861835 3.3324234 30.366947 55-59
GCAGC 2581995 3.2924125 22.86416 20-24
AGTAG 4149890 3.2809734 33.6243 9
ACGGA 3458155 3.2396312 31.803608 45-49
AACTT 5226435 3.1480517 68.92291 7
GCGTT 2476190 3.0611215 39.44324 15-19
CTTGT 3077685 3.0507977 113.30619 9
TTCTC 3238755 3.0503163 30.87369 30-34
AGAGC 3194025 2.9921918 52.019585 95-97
TGCGT 2393740 2.959195 42.36417 15-19
ATAGC 3935535 2.9562929 25.590302 80-84
ACCAT 4120920 2.9411387 32.858734 75-79
AACCA 4729625 2.9384973 20.081354 70-74
CCATG 2850615 2.914674 35.413845 75-79
ACCTG 2813360 2.876582 27.498062 15-19
AGGAG 2916150 2.8753016 23.522156 90-94
TTCAC 3461585 2.8380435 23.04941 45-49
CATGC 2766110 2.82827 35.157356 75-79
GAGCC 2170750 2.768016 62.617466 95-97
AGAAG 3979775 2.7390623 20.306772 6
TGCAT 3145410 2.714212 27.649675 75-79
GCTGC 1842155 2.6984088 37.429092 1
AAGTT 4252435 2.6958542 22.83519 60-64
CACTG 2603810 2.6623228 34.319817 85-89
TTTCA 3811250 2.6370995 22.824814 40-44
ACAGG 2799445 2.6225455 15.178563 90-94
CTCAG 2564625 2.6222572 35.307472 35-39
TGAAG 3295675 2.6056168 20.861109 60-64
TTGAA 4070935 2.5807915 20.614906 55-59
GCACT 2509900 2.5663025 25.863634 85-89
GAGAG 2593765 2.5574324 40.77233 95-97
AGCAC 2872020 2.5563254 27.951824 80-84
GACTC 2442895 2.497792 34.966484 25-29
GTTGT 2335335 2.4364686 16.703085 35-39
GGAAC 2590730 2.4270196 32.203697 70-74
TTGTA 3314835 2.4140344 31.951468 9
GGATT 2621675 2.3810458 23.664936 50-54
GATGA 3000595 2.3723214 11.844501 15-19
GTAGG 2080900 2.3569343 25.659163 7
AAACT 4471640 2.344663 33.955208 6
GTAGT 2555390 2.320845 23.694832 40-44
CATTC 2827505 2.3181813 28.628435 30-34
GTTAC 2682080 2.3143988 27.3016 60-64
TTAGC 2669675 2.3036945 30.098618 20-24
ATGCA 3064090 2.3016815 26.054068 75-79
GGAGG 1627090 2.298345 19.963642 90-94
TCTCA 2786430 2.2845054 21.637964 35-39
ACCAG 2562880 2.2811663 15.118839 55-59
AGTTT 3129280 2.278903 25.572592 40-44
GAAGA 3292875 2.2663064 10.338156 50-54
GGCTC 1545050 2.2632062 23.328663 65-69
CAGAC 2530350 2.252212 30.568668 25-29
CTGAA 2996850 2.251172 25.195665 85-89
TCAGT 2606255 2.2489684 29.963892 35-39
CAGTA 2993355 2.2485468 25.842457 35-39
TGTTG 2155040 2.2483659 15.053093 35-39
CAGCA 2494975 2.2207253 18.667562 20-24
CTCAT 2705365 2.2180429 21.498919 30-34
CTGCA 2155520 2.203959 21.472633 3
TTTCC 2329960 2.1943972 14.1494465 40-44
CAGAA 3354040 2.1932554 15.329827 6
GTTAG 2402670 2.1821423 31.487228 20-24
GCATA 2893430 2.1734853 19.514027 80-84
CAGGC 1694025 2.1601236 17.722265 65-69
TAACC 3022895 2.1574678 24.509424 10-14
AAGGA 3133800 2.1568239 18.062054 70-74
CATAG 2864025 2.1513968 25.698822 80-84
CCATC 2209360 2.1463249 15.954613 75-79
TGTAA 3382095 2.1440978 22.3552 10-14
TAGAG 2703510 2.137441 26.100462 90-94
AGGAA 3082765 2.1216993 23.823458 70-74
AAGAG 3061945 2.10737 11.391425 50-54
TCATT 3035265 2.100176 24.057205 30-34
CGTTA 2421640 2.0896618 22.31717 20-24
AACCT 2927190 2.0891626 23.10877 10-14
GGAGC 1548255 2.0778956 21.512207 95-97
CCAGA 2312405 2.0582237 19.40633 55-59
AGGGA 2076845 2.0477533 22.390942 9
CTAAG 2723410 2.0457697 25.558094 65-69
ATTCT 2927125 2.025351 24.206703 30-34
GTAAC 2680990 2.0139048 26.070541 10-14
TAAGG 2538270 2.0067992 24.989342 65-69
TAGGG 1763670 1.9976233 25.557194 8
TGCAG 1855700 1.9970163 24.582026 4
TGAAT 3122110 1.9792788 20.41684 85-89
AGTTA 3100280 1.9654393 21.619215 60-64
TTCCG 1672920 1.9649404 18.22716 40-44
TAGTT 2687965 1.9575148 25.157755 40-44
TTGTT 2326825 1.9465609 14.023034 35-39
ACTGA 2567940 1.9289837 25.4441 85-89
AGACT 2562025 1.9245405 26.002033 25-29
GATGT 2115745 1.9215523 18.438198 30-34
GGATG 1687110 1.9109076 19.42353 10-14
ACTCA 2649210 1.8907657 22.872137 25-29
GAATA 3424045 1.8896241 14.36953 90-94
ATGAT 2973945 1.8853486 10.502147 15-19
TTAAA 4247015 1.8793706 15.661727 50-54
TTACT 2694135 1.8641397 17.943718 65-69
TGATG 2043390 1.8558385 14.879397 30-34
AATAG 3325705 1.8353536 18.989565 90-94
GGAAG 1857505 1.8314857 10.736971 6
TCCGT 1558995 1.831129 18.77139 40-44
CAGAG 1933235 1.8110721 14.823539 25-29
TGTTT 2154440 1.802348 17.255081 35-39
CAGGA 1893475 1.7738247 14.145555 90-94
TCCTC 1571740 1.7540115 8.631613 1
AGGCT 1622900 1.7464879 16.62395 65-69
CCAAA 2802825 1.741384 26.663836 4
GCTCA 1701250 1.7394805 20.574781 65-69
ATTAA 3930080 1.7391218 15.672843 50-54
AGCTG 1608410 1.7308946 9.339725 95-97
GAGAT 2163840 1.7107686 16.096518 25-29
CTACA 2380660 1.6990991 12.101575 85-89
TACTA 2805885 1.6900759 20.702425 65-69
TACAG 2248610 1.6891096 11.413004 90-94
CCTCC 1273545 1.6840348 6.0915685 1
TAAAC 3203505 1.6797282 17.046686 50-54
GAGGA 1701545 1.6777104 20.127556 95-97
CATCA 2342855 1.6721172 10.064082 80-84
CTCAC 1716695 1.6677161 15.229129 65-69
GCGTC 1137070 1.6655926 5.256522 95-97
AAGAA 3461565 1.6629751 10.663348 45-49
GGGAT 1461550 1.6554267 22.73521 10-14
GAGCT 1527420 1.6437368 11.313263 95-97
ATACC 2285825 1.6314143 11.134001 75-79
GAGCG 1214555 1.6300406 7.7301574 90-94
TACCA 2277175 1.6252409 11.553416 75-79
GATTA 2552030 1.6178733 21.731554 50-54
TGACC 1572005 1.6073313 42.98158 1
CGTCG 1096215 1.6057477 5.447673 95-97
ACCAA 2569790 1.5966003 13.801812 3
AACAG 2433545 1.5913304 15.6204195 60-64
GATCG 1465300 1.5768863 5.473564 95-97
TCTCC 1408385 1.5717126 5.1490803 1
ATAGA 2824970 1.5590132 18.86523 90-94
TCTAC 1865745 1.529665 15.530881 85-89
GAAAC 2321085 1.5177912 10.348918 60-64
ACTAA 2888755 1.5146918 18.22423 65-69
GTAAA 2714955 1.4982996 8.755048 45-49
TAAAG 2698610 1.4892793 9.693737 45-49
GAGAA 2163285 1.4888712 14.216366 50-54
CCGTA 1455360 1.488065 20.411219 40-44
ATGTT 2036990 1.4834411 11.577274 30-34
ATCGG 1369885 1.4742051 5.0136347 95-97
AGCGT 1367580 1.4717247 5.923498 90-94
TGCTG 1175825 1.4535813 7.7006116 1
AAAGA 3023815 1.452675 8.366802 45-49
AGCCA 1609275 1.4323823 13.35117 95-97
AGATG 1793335 1.4178411 12.517408 25-29
AGAAA 2928430 1.4068509 7.3096147 60-64
CGGAA 1489690 1.3955553 8.953538 5
CTGCT 1182295 1.3886731 5.7901006 2
GATAC 1838865 1.3813176 10.145909 75-79
CAAAC 2195830 1.3642603 31.46774 5
GGGGG 673930 1.3637935 6.6783295 1
TCTTG 1374260 1.362254 5.31559 4
AAGTA 2466735 1.3613144 24.509966 8
CTCTT 1436755 1.3531611 5.9144135 3
GACCA 1509670 1.343726 17.404562 2
TGTAG 1469415 1.3345455 5.528473 3
GCTCC 958140 1.3334849 5.8259487 1
AGAAC 2008260 1.3132304 11.396856 50-54
TCAAT 2149805 1.2948973 9.3222275 80-84
CAATA 2465430 1.2927253 10.256147 80-84
CAACA 2040125 1.2675215 6.5592527 8
CGTAA 1681780 1.2633185 11.792539 40-44
GGCCA 975570 1.2439911 15.774568 2
AAACA 2718630 1.2409118 7.7563467 60-64
GATAG 1569360 1.2407626 15.925149 15-19
GCCAA 1380175 1.2284652 13.152876 3
ATCAA 2330240 1.2218397 8.163996 80-84
TGATA 1925500 1.2206813 10.4453335 15-19
TATCT 1746505 1.2084506 10.238693 85-89
GCTGG 777795 1.1991379 6.918997 1
CCTCT 1070890 1.195079 6.7627673 2
GGATA 1472230 1.16397 12.112972 70-74
ATCTA 1912295 1.1518375 9.397678 85-89
CTTTT 1382485 1.0988597 5.518428 6
CCAAC 1292820 1.0933126 5.951783 5
CCGAA 1224825 1.0901914 27.590015 4
CCCAA 1283595 1.0855111 8.042549 4
TGGCC 735910 1.0779692 29.88301 1
TCAAC 1506850 1.0754527 7.636075 7
GCCCA 886480 1.0740026 6.643633 2
TGCCA 1044620 1.0680948 5.417158 1
CCTTT 1124130 1.0587255 5.1052647 4
AATAT 2353985 1.0416752 6.789769 80-84
GTTGC 836060 1.0335561 7.1362157 1
ATATC 1683450 1.0139966 8.077274 85-89
CCATA 1402115 1.0007025 5.3371944 3
GGTGC 617765 0.9524173 10.917253 2
CTCCG 673090 0.93676853 5.7054687 2
GTCCA 913585 0.9341152 5.0220275 2
TGTCC 791200 0.92930967 9.263717 1
TAAGT 1464940 0.92870665 5.200386 5
CAACT 1298685 0.9268835 5.206104 6
CCGTT 787185 0.92459387 5.250071 4
GTGCA 856665 0.92190236 8.438636 3
GGCTG 593415 0.9148766 9.896783 1
CCTAA 1275160 0.9100935 10.179494 4
ACCTA 1263720 0.90192866 6.8025513 3
CTAAA 1706750 0.8949185 5.610007 4
GAACT 1184135 0.8894979 24.385075 6
GCTGT 709895 0.8775881 9.589927 1
GGGTG 538555 0.8738895 12.457765 1
TGCCC 627885 0.87385476 8.276386 1
CGAAC 979260 0.871619 25.990532 5
GACCT 850915 0.8700368 9.587191 2
CTGTC 737015 0.8656662 9.389045 4
ACCGA 970415 0.8637463 16.115608 3
ACCCA 998410 0.8443358 5.047702 3
TGTCA 948345 0.8183382 7.48374 5
GGCCT 549965 0.80559474 7.7532144 2
AACAA 1757325 0.8021266 5.23311 9
GCAGG 590315 0.79225516 6.044592 1
CCGTC 566185 0.7879842 5.8555984 4
AAGTC 1042235 0.7829055 6.09554 6
TGGCT 625410 0.7731459 7.244777 1
AGTCT 870245 0.7509447 8.774102 7
GTCAA 995560 0.7478443 7.6578646 6
GCCAG 579620 0.7390983 5.118584 2
GGGCC 396635 0.72456944 9.399334 1
TGCGG 437970 0.6752247 13.824608 3
TCCGA 651400 0.66603833 5.101406 3
TCTAA 1086900 0.65467525 6.464217 9
GGCCG 348465 0.6365729 15.95546 2
CTAAC 874130 0.6238746 9.302636 5
CAGGG 462185 0.6202933 5.4674006 2
TGACT 711785 0.6142078 12.248384 1
GACTG 568225 0.6114968 8.422433 2
TAACT 1014970 0.6113494 8.387976 6
GACCG 477790 0.6092505 22.335808 2
GTCTA 704695 0.60808975 8.794917 8
GCCGA 473375 0.60362077 11.794914 3
GACTT 676785 0.58400583 8.603968 6
GCCCC 351325 0.5793662 5.64707 1
GACCC 473625 0.57381386 6.862873 2
GGCCC 324205 0.56271213 6.6719623 1
CCCCG 338360 0.5579858 5.603582 2
GCGGA 403835 0.5419824 11.219144 4
GCCCG 284155 0.49319863 6.3078566 2
GTCCG 331825 0.48606095 6.1393237 2
CCCGA 399840 0.48442063 5.5439153 3
CCCGT 341240 0.4749185 5.3368187 3
Produced by FastQC (version 0.10.1)