FastQCFastQC Report
Thu 31 Jul 2014
20S15B_CTTGTA_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20S15B_CTTGTA_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12096099
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 229588 1.8980334072993286 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 101488 0.839014297088673 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 85032 0.7029704369979115 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 82360 0.6808806707021826 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 79719 0.659047185377699 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 72865 0.602384289348161 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 67026 0.5541125283448821 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 58573 0.48423049447594635 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 56312 0.4655385178312446 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 55704 0.46051210394359376 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 51253 0.4237151167496232 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 46610 0.38533084095955233 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 43475 0.35941339435135244 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 42737 0.35331225381009196 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 42327 0.34992273128716955 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 41798 0.3455494205197891 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 39214 0.3241871614972728 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 38450 0.31787107562529043 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 36517 0.30189071699892667 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 35554 0.29392947263411123 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 35409 0.2927307390589313 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 35304 0.29186269060793896 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 32555 0.269136355448149 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 30693 0.25374296291721815 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 30383 0.2511801532047646 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 30043 0.24836932964917036 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 29680 0.24536836214716826 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 27299 0.22568433012990388 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 27065 0.22374982215340664 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 26708 0.22079845742003268 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 26495 0.21903755913373393 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 25765 0.213002555617311 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 24449 0.20212301503154034 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 23685 0.19580692915955794 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 23597 0.19507942188634533 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 23283 0.192483543661473 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 22307 0.1844148266312966 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 22251 0.183951867457434 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 21380 0.17675119887824992 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 20740 0.171460236891249 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 20123 0.16635941885065592 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 19898 0.16449931502710088 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 19759 0.16335018422054912 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 19662 0.1625482727943943 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 19567 0.16176289562444884 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 19278 0.15937369560219372 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 18152 0.15006490935631395 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 17925 0.14818827127654957 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 16678 0.13787916253000246 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 16670 0.13781302550516492 No Hit
ATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGAC 16072 0.13286928289856093 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 15814 0.1307363638475512 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 15729 0.13003365795865263 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 15154 0.12528005929845648 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 15137 0.12513951812067678 No Hit
TACCATGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTC 15121 0.12500724407100175 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 14218 0.11754202739246761 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 14017 0.11588033464342512 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 13487 0.11149875674793999 No Hit
ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC 13429 0.11101926331786802 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 13036 0.10777028197272524 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 12917 0.10678649372826726 No Hit
ACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGACTG 12888 0.10654674701323129 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 12739 0.10531494492563263 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 12728 0.10522400651648105 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 12461 0.10301668331252911 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 12420 0.10267773106023687 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 12184 0.10072668882753026 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 4371105 3.6464088 5.21233 70-74
CTGCT 3832705 3.4934363 10.734571 4
ACTTC 3940555 3.3858998 6.9286904 60-64
AACTT 4393140 3.101029 5.9531064 60-64
AGCTG 3169875 3.0199442 7.7070756 3
CTACT 3417770 2.9366999 13.182806 2
AGCAC 2228740 2.9223764 5.2109995 35-39
GGAAG 2934665 2.9222991 6.505412 15-19
CTTCT 3929645 2.8151753 6.010844 40-44
CAACT 2711495 2.7944107 8.261155 9
TCAAG 3003065 2.6970615 5.570484 10-14
CAAGC 2054345 2.6937056 8.470431 75-79
TGCTA 3554290 2.6614254 7.578332 4
TTCAA 3749235 2.6465094 7.4694715 55-59
CTGGT 3317805 2.6353784 10.22085 1
CTTCA 3056305 2.6261134 6.884217 60-64
GCTGC 2254960 2.6150596 8.576585 3
TCTCT 3609750 2.5860043 8.683535 20-24
ACTGC 2314305 2.5300705 10.8412075 2
ACAAC 2040380 2.5220697 8.148258 85-89
CAGCA 1908875 2.5029619 6.293622 2
TCAAC 2420945 2.4949758 7.317904 55-59
AGGAA 2654490 2.4918191 6.5282865 40-44
CTGAA 2769595 2.4873815 6.514717 80-84
ACCCA 1613025 2.4270186 40.26391 1
ACATC 2346790 2.418553 6.4944687 90-94
CGGTG 2389310 2.4146824 10.507447 9
GCAGC 1732055 2.4091792 6.846829 1
TGGTG 3411160 2.3612335 7.97704 8
GGTGT 3399830 2.3533907 9.388977 9
GCTAC 2143665 2.3435218 16.399605 1
CAACA 1885245 2.3303108 7.8213215 85-89
AAGCT 2583870 2.320581 5.700918 75-79
TATGC 3082220 2.307943 7.384149 8
GAAGC 2008215 2.2947302 7.4209127 15-19
GGTGG 2568505 2.262104 8.259823 7
CCAAG 1709025 2.2409139 7.6536293 75-79
TGAAA 3018290 2.226902 5.128779 70-74
ATGGT 3410930 2.225763 5.7775917 1
CAAGG 1910295 2.1828399 6.944738 10-14
CAGCT 1980530 2.1651773 5.72905 2
CTTTA 3673850 2.1621585 6.312065 5
CTGCA 1969540 2.153163 5.3624187 95-97
CCAGC 1341510 2.141197 6.8299994 1
CGGAA 1868440 2.1350133 5.872366 95-97
CCGTG 1811620 2.1009214 5.586792 60-64
ATGCA 2337670 2.0994682 5.867214 1
CATGG 2198070 2.0941043 13.581183 2
TCGGA 2195480 2.091637 6.221646 85-89
CGTGA 2193995 2.0902221 11.220498 9
CTCCT 1997215 2.0889475 7.284808 70-74
ATCGG 2191390 2.0877402 5.9570932 85-89
TCCTT 2844330 2.0376618 5.8445945 40-44
CTTGG 2543140 2.0200512 7.3267374 6
CTCTG 2214515 2.0184875 7.559776 20-24
GCTGG 1984095 2.0051641 8.635611 4
GCTTC 2194700 2.0004263 8.056501 15-19
GCTCC 1500945 1.99739 5.7435546 70-74
TTTCA 3270220 1.9246114 5.7532077 8
TAGCT 2556985 1.914651 5.658803 2
AAGGA 2034565 1.9098842 6.322665 10-14
CCGCC 961590 1.8682655 6.8646173 45-49
GGTAT 2860290 1.8664492 6.318546 6
CCTGT 2025260 1.8459848 7.725614 1
CACAA 1493015 1.8454837 7.014707 85-89
TTCCA 2141295 1.8398961 5.5720887 75-79
TTCGG 2310025 1.8348849 8.216927 7
CCTTA 2129875 1.8300833 7.660378 95-97
TGGCT 2303210 1.8294716 6.095413 9
TGCTG 2269335 1.802564 5.5417366 6
CTATG 2389280 1.7890748 5.576545 35-39
GCACA 1357515 1.7800056 5.067658 95-97
GCCTT 1952270 1.7794563 5.027903 35-39
TCGGT 2238495 1.7780676 9.674654 8
CATCC 1414160 1.7740504 7.2212224 90-94
TATTC 3011090 1.7721063 5.392328 5
CCCAA 1169075 1.7590346 40.12094 2
CTTTC 2453800 1.7578883 6.8288655 7
TGTAG 2592360 1.6916146 5.7707024 3
TTGGT 3102545 1.68795 16.637075 7
TACTG 2243125 1.679635 5.1954713 3
CCAAT 1613270 1.6626025 27.704357 3
AGAAA 1877570 1.661502 7.8257775 9
CACCC 907135 1.66146 5.8605814 50-54
TACTT 2803790 1.6501048 7.887656 3
CCACA 1091155 1.6417933 7.767579 1
CACAC 1089340 1.6390623 5.7519403 95-97
GCACC 1024105 1.6345838 5.301709 50-54
TGGTA 2502990 1.6332974 8.144116 2
GTGGC 1614835 1.6319829 7.211041 8
TGAAC 1814445 1.6295583 6.154414 80-84
CTGTA 2172295 1.626598 6.899955 8
AATGA 2202175 1.6247703 5.284296 75-79
AGCCT 1459805 1.5959045 6.257967 2
GCAAC 1202430 1.5766547 7.836358 1
GCTTG 1984050 1.5759583 6.112948 1
ACGGT 1652125 1.5739819 9.396822 4
GTGTA 2398065 1.5648298 8.459188 2
GAACA 1447370 1.5590868 6.185451 80-84
CATGC 1416830 1.5489229 6.949057 2
ACACA 1249340 1.5442823 6.7263746 85-89
ATGCC 1403830 1.5347109 10.01453 9
TTTAG 2991085 1.5340501 5.348779 6
GCTGT 1926790 1.530476 5.681889 7
TCCAA 1484835 1.5302402 5.894965 75-79
GCTGA 1591885 1.5165911 5.592028 80-84
ACCGC 948535 1.5139658 5.0182257 45-49
GTAGC 1579855 1.50513 6.349524 1
TTAGG 2299380 1.5004339 5.33516 35-39
AAGCC 1135105 1.4883764 7.262996 1
ACTTT 2523140 1.4849348 8.153536 4
CATGA 1633445 1.4670017 5.000411 65-69
ATGGG 1761040 1.4620785 11.533603 3
AACAC 1181075 1.4599013 6.8238435 85-89
GGTCG 1434730 1.4499655 11.453892 6
ACATG 1598835 1.4359185 13.9117775 1
CGCAG 1023270 1.4233041 5.1840186 90-94
ATTCC 1656155 1.4230419 5.117377 80-84
ATCTC 1635980 1.4057066 5.4642644 45-49
GGTAC 1469135 1.3996469 9.305847 3
GACGG 1148500 1.3921406 11.878766 3
GTCAA 1523505 1.3682642 5.160477 10-14
GTATT 2665690 1.3671634 5.333614 4
GACTG 1424415 1.3570421 8.697111 1
GCGAC 970510 1.3499182 5.0751634 20-24
GGCTG 1334185 1.3483527 5.6978 9
GTCTG 1693550 1.3452101 5.1775317 90-94
GCGGC 897515 1.3242801 7.16366 7
GGTCA 1380350 1.3150615 23.193277 9
TGGGT 1892355 1.309904 8.494098 4
GGGTC 1295800 1.3095601 11.355425 5
TTCTC 1825590 1.3078423 5.011824 20-24
GCCTG 1122755 1.3020504 6.1076627 6
TGGTC 1595785 1.2675542 19.591877 8
CAATT 1765605 1.246305 19.174482 4
GTATG 1904650 1.2428575 6.4842916 7
TGTAT 2396820 1.2292669 5.5265346 9
CTGAC 1119505 1.223878 10.886256 1
GTACT 1583605 1.1857914 7.31482 4
TGACG 1244590 1.1857227 9.2032995 2
CACAT 1141520 1.1764268 5.1798983 2
TGGGG 1319525 1.1621168 5.517366 8
ATTCG 1505585 1.1273706 6.5761876 6
ATTGG 1666280 1.0873117 16.745285 6
TGGCC 930500 1.0790937 8.755075 1
CTTAT 1811120 1.0658923 5.2582765 95-97
GGGGT 1197565 1.0547057 5.121195 9
TCGTG 1317280 1.0463337 9.05976 8
TGCGG 1021120 1.0319632 5.260824 6
CGGTA 1067450 1.0169611 8.835267 5
GCCTA 907725 0.99235344 6.0702934 3
TAGAA 1337195 0.98658574 6.2472076 8
AATTG 1564420 0.9623406 15.200602 5
CGGCT 827120 0.9592047 5.4730067 8
TTAGA 1323535 0.814162 5.6818123 7
GGCCT 683825 0.79302657 5.881119 5
CTACA 760395 0.78364736 5.8787713 3
CACGG 537875 0.7481502 5.0270805 4
GTCGT 928045 0.7371589 8.85184 7
GGCCC 405515 0.6865945 5.153814 1
Produced by FastQC (version 0.10.1)