FastQCFastQC Report
Thu 31 Jul 2014
20S15A_CTTGTA_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20S15A_CTTGTA_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 16486326
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 597328 3.623172318683981 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 296416 1.7979506167717416 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 239989 1.4556851538663012 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 192355 1.1667548003114825 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 170829 1.0361859883154074 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 121341 0.7360099515198232 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 108128 0.6558647451227156 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 105380 0.6391963861444934 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 73672 0.4468673008164463 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 67943 0.412117290413886 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 65033 0.3944662989194803 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 58505 0.3548698478969784 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 55547 0.33692770602740724 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 54960 0.33336717956444634 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 50805 0.3081644752141866 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 47775 0.28978560778186724 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 44882 0.2722377320453326 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 44496 0.26989639777837704 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 43150 0.2617320560081124 No Hit
CACAGTCAATTTGTCTTCCTGCCCTCCATCCAGCGTTTCAGTAGTACCGC 40940 0.24832700748486958 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 40580 0.24614337967112868 No Hit
AACAAGTTTATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATT 37346 0.2265271231443561 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 36837 0.2234397160410391 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 36453 0.22111051303971546 No Hit
CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA 35701 0.21654915716212333 No Hit
CATCTTTTCAAGAGTTATTAATTGAGTTTTAGGGAGGGATTTATGTCACC 35564 0.21571816546633857 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 35051 0.2126064958317578 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 34851 0.21139336926856836 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 33365 0.20237983890407119 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 32526 0.19729077297149164 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 32315 0.19601092444732682 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 32139 0.19494337307172016 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 29605 0.1795730595161105 No Hit
ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC 28196 0.17102658287844122 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 28113 0.1705231353547176 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 27729 0.16819393235339397 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 27385 0.1661073546647082 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 27369 0.16601030453965304 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 26937 0.16338995116316396 No Hit
GGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGACTGCTACTT 26744 0.1622192840296862 No Hit
GCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCAAT 26590 0.16128517657603034 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 25922 0.15723333385497776 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 25225 0.1530055877822627 No Hit
AGTATGCTTCAAAAATACCTTTGGTATTACATTGACAATCTAACGAGGAT 24376 0.14785586552152372 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 23727 0.14391926982397413 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 22655 0.13741691144527896 No Hit
ACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGAT 22409 0.135924765772556 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 22137 0.13427491364661842 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 21676 0.13147865691846686 No Hit
CTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGC 21020 0.12749960179120565 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 20945 0.12704467933000962 No Hit
ATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGAC 20784 0.12606811244664215 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 20685 0.1254676147978634 No Hit
GGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAACTTAAACGGTTTC 20627 0.12511580809453848 No Hit
TACCCATTAGATTTATTTGAAGAAGGTTCTGTTACTAACTTATTTACTTC 20559 0.12470334506305407 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 20216 0.12262283300718424 No Hit
CCTACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGC 20085 0.1218282351082952 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 19709 0.11954755716949914 No Hit
GTATGCTTCAAAAATACCTTTGGTATTACATTGACAATCTAACGAGGATT 18691 0.11337274296286509 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 18243 0.11065533946132086 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 17995 0.10915106252296601 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 17758 0.10771350754558656 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 16897 0.10249099769105621 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 16816 0.10199968143296449 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 16736 0.10151443080768875 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 8902200 5.1184444 14.805623 70-74
AGCTG 5114815 3.6703138 11.084073 80-84
CAAGC 3773780 3.543589 14.477152 30-34
CTGCT 5119030 3.4187279 20.670742 3
ACTTC 5464975 3.309701 9.9597225 60-64
GGAAG 3939350 3.0373454 11.346174 15-19
CTTCT 5939935 3.0338023 8.456473 20-24
ACCCA 2903415 3.0086696 71.03572 1
AACTT 6011075 2.9914227 8.215874 60-64
CTGGT 4885955 2.956839 20.732607 1
CTGAA 4482095 2.9166074 10.010495 80-84
ACAAC 3422105 2.9139616 13.77723 85-89
AAGCT 4448725 2.8948927 9.757765 75-79
AGGAA 4083130 2.8548713 10.594381 40-44
CCAAG 3025110 2.840586 13.004746 75-79
CAACT 3869085 2.7784574 17.362514 9
TCTCT 5430195 2.7734542 10.243114 20-24
AGCAC 2927465 2.7488976 8.763823 65-69
ACATC 3803815 2.731586 10.434075 90-94
CAACA 3149655 2.6819673 13.063882 85-89
TGGTG 4880880 2.676565 6.3173866 8
CTTCA 4414980 2.6738024 9.763248 60-64
GGTGT 4735225 2.596691 7.6362205 70-74
TCAAG 3950120 2.5704384 9.410453 10-14
CGGTG 3244920 2.567755 9.754564 85-89
CTACT 4224205 2.558265 17.311012 6
TTCAA 5112805 2.5443969 16.362944 9
TCAAC 3529820 2.5348253 15.475284 8
GCTGC 2878095 2.513352 10.838456 3
GAAGC 2949360 2.509551 12.533716 15-19
TATGC 4504895 2.472217 9.537201 30-34
GGTGG 3386160 2.4280546 5.9561687 90-94
CTTTA 5778440 2.425165 10.982718 9
GCTAC 3056055 2.4200983 21.928423 5
GCTTC 3620315 2.417816 11.475057 15-19
CAGCA 2572625 2.4157019 10.018205 60-64
TGCTA 4286400 2.3523104 15.899325 4
TTCCA 3860575 2.3380435 9.188425 75-79
GCAGC 2240220 2.3197105 7.7604523 1
ACTGC 2910195 2.3045912 22.97564 2
CCAGC 1999665 2.285067 10.326351 60-64
CTCTG 3421240 2.2848642 11.380681 20-24
CAAGG 2677175 2.2779539 12.036427 10-14
CACAA 2668755 2.2724755 11.786394 85-89
GGTTC 3731005 2.2578967 6.131281 90-94
TCTGC 3365525 2.2476552 6.6781554 45-49
GAAAG 3176380 2.2208834 7.848468 10-14
CATCC 2497000 2.182172 11.908913 90-94
GCTGG 2756130 2.1809678 9.294135 75-79
TCCTT 4215280 2.1529403 7.553516 90-94
TGAAA 4013390 2.1460183 6.7899866 65-69
GTTCC 3200495 2.1374404 8.950617 70-74
CTTGG 3500245 2.1182475 7.411875 65-69
ATGCA 3206160 2.0863256 6.057597 1
CCCAA 1999310 2.071789 70.06626 2
AAGGA 2961215 2.070443 10.451361 10-14
ATGGT 4152445 2.0649393 5.1809497 90-94
CAGCT 2586755 2.0484583 6.9594884 2
ACACA 2391505 2.036394 11.608449 85-89
TTTCA 4837250 2.030155 14.02118 8
ATCGG 2813085 2.0186272 7.9854665 85-89
GCCTT 3020770 2.0174117 9.037204 35-39
GATGG 3074970 1.9994731 6.4421883 90-94
CCTTA 3295385 1.9957527 13.951265 95-97
TTGGT 4741895 1.988659 29.793741 7
CCGCC 1422785 1.9785904 11.576754 75-79
GAAAA 3119470 1.9778702 7.371981 70-74
GAACA 2552225 1.9692955 10.77512 80-84
TACTT 4676330 1.9626181 16.306284 3
TCGGA 2725880 1.9560502 8.397137 85-89
AACAC 2296950 1.9558792 11.7088585 85-89
TGAAC 2979370 1.938748 9.461301 80-84
CACCC 1524825 1.9229159 13.49976 50-54
ACCAG 2045955 1.921157 8.663374 60-64
GCTGA 2674955 1.9195071 9.990417 80-84
TCCAA 2665475 1.9141244 9.879297 75-79
GCACC 1670535 1.908962 12.39884 50-54
TGCTT 4123730 1.9085221 6.772167 65-69
CTTTC 3728585 1.9043626 16.755436 7
TCGGT 3145390 1.9034994 8.00189 95-97
TTCGG 3134165 1.8967063 8.138997 95-97
GCTTG 3131095 1.8948485 9.156536 65-69
AGAGC 2224105 1.8924462 5.2097273 95-97
TGGCT 3116455 1.8859888 7.11038 90-94
CTCTC 2552775 1.8814297 8.156752 95-97
CCACA 1804280 1.8696887 11.335485 60-64
GCAAG 2190535 1.8638821 9.052882 25-29
CATGA 2843160 1.8501128 8.7744665 65-69
TCTGG 3042965 1.8415147 8.517745 45-49
CATGG 2564665 1.8403647 12.016138 2
ACTGA 2824500 1.8379703 5.9277267 65-69
GGTAT 3694605 1.837263 7.736821 30-34
AAAGA 2896745 1.8366536 6.1974344 15-19
AGAAA 2888800 1.831616 13.029383 9
TGGTA 3681395 1.8306942 16.861082 2
GCACT 2291845 1.8149183 6.947152 65-69
CGTGA 2514350 1.8042594 8.634297 9
GGCTC 2064995 1.8032967 9.484411 90-94
GGTAC 2504455 1.7971587 23.029306 3
GTGTA 3609535 1.7949594 6.0601444 2
ATGCC 2265390 1.7939684 10.54327 30-34
ACTTT 4263700 1.7894406 16.607819 4
CCAAT 2458440 1.7654487 48.81858 3
CATGC 2220095 1.7580992 6.614374 2
CCTTT 3416395 1.7449125 7.105544 35-39
ACGGT 2431335 1.744689 9.539265 25-29
TTTAG 4567250 1.736948 8.966765 6
TGTAG 3489605 1.7353203 5.9230103 70-74
AACAT 2919385 1.7227093 8.378899 90-94
AAAAC 2458795 1.7204334 7.131321 70-74
AGCTT 3127975 1.7165847 8.014869 15-19
AAACC 2014145 1.7150675 7.828351 70-74
CACTG 2150975 1.7033628 7.462976 65-69
AGCCT 2146520 1.699835 7.1647115 30-34
GTGGC 2144135 1.6966867 5.641304 90-94
TGCTG 2801135 1.6951662 5.58646 3
AAAGC 2175735 1.6787958 8.0184965 20-24
GACTG 2332550 1.6738025 19.16305 1
CTATG 3046300 1.6717627 6.525134 35-39
GTAGC 2327335 1.6700603 5.183845 75-79
TGCAC 2107080 1.6686023 8.823017 50-54
ACCGC 1459370 1.6676584 9.375471 75-79
AAGCC 1764875 1.6572224 8.186784 30-34
GGTCG 2085690 1.6504382 9.442472 6
ATGAT 3624255 1.6343527 6.493467 65-69
GTGTT 3894680 1.6333535 7.104601 70-74
TCATG 2967720 1.6286391 7.60746 60-64
TTACA 3259400 1.6220465 5.758571 80-84
GACGG 1724780 1.6183717 12.502574 25-29
ATGGG 2473960 1.6086714 9.243776 3
TTCAT 3822070 1.6040921 6.442019 60-64
CTGCG 1834145 1.6017025 8.794736 45-49
GGATG 2445200 1.5899704 7.0731163 85-89
ACATG 2419975 1.5747361 11.693752 1
TTAGG 3161865 1.5723408 8.426791 35-39
GCTCT 2332240 1.557579 7.288718 90-94
GATCG 2165330 1.553808 5.2458467 8
ATCTC 2532190 1.5335462 10.176768 45-49
GCGAC 1465600 1.5176045 8.675344 50-54
TGGTC 2497625 1.5114907 38.512638 8
GGGTC 1897480 1.5015049 9.406705 5
CTCTA 2473310 1.4978873 10.64641 95-97
GTCAA 2288940 1.4894685 8.865221 10-14
GACGC 1426480 1.4770963 9.1914 15-19
GTACT 2686055 1.4740658 17.82197 4
GCTGT 2429710 1.4703904 5.138857 4
TCCAC 1675515 1.4642619 6.16561 60-64
GCGCA 1412990 1.4631277 9.188304 25-29
GGTCA 2036210 1.4611533 45.366577 9
ACTGG 2034095 1.4596357 7.2343483 50-54
TACCA 2030035 1.4578037 6.110984 60-64
AATCT 2919025 1.4526583 7.4599814 40-44
AGCGC 1398210 1.4478232 8.6774845 25-29
CTGGG 1829310 1.4475609 6.8027053 55-59
TGGGT 2639490 1.4474369 7.116323 4
CATCG 1822110 1.4429338 6.770194 85-89
GATTG 2901240 1.4427366 6.879336 65-69
ATCCT 2379540 1.4410981 8.187335 90-94
TTCTC 2817680 1.4391208 7.4431014 20-24
GTATG 2883780 1.434054 6.924906 30-34
AAACT 2428220 1.4328761 5.771413 6
GGAAT 2425700 1.4303272 8.195142 40-44
GCGAA 1677230 1.4271213 7.30471 20-24
ATGCT 2551040 1.3999716 5.3295493 3
CTGTA 2536050 1.3917453 6.4385853 8
CAATT 2792625 1.389755 34.298138 4
CGCCT 1437590 1.385421 8.654646 75-79
GAATC 2127905 1.3846793 8.786608 40-44
CGCAA 1473100 1.3832449 8.303668 25-29
ATTCC 2281275 1.3815868 6.827237 55-59
TTGTG 3288170 1.378995 5.9472556 70-74
TCTGA 2504135 1.3742309 8.428216 20-24
CTGAC 1730890 1.3706964 10.524834 25-29
TGGGG 1909310 1.3690755 6.507319 35-39
AAGAC 1773455 1.3683969 6.8635626 15-19
TGCCT 2027785 1.3542497 8.92855 30-34
AAGCG 1591450 1.3541328 7.2048717 25-29
CCCAT 1541660 1.3472835 6.1210294 55-59
GCAAC 1434790 1.3472717 9.275588 1
AACCG 1429755 1.3425436 9.039482 70-74
TGACG 1868380 1.3407211 9.681309 25-29
ACAAG 1729480 1.3344657 5.0578322 2
TGATT 3508205 1.3341881 5.3304014 65-69
TCGCT 1982270 1.3238527 6.0627956 40-44
CACAT 1838620 1.3203452 7.844696 60-64
CTTAT 3138505 1.3172053 9.326543 95-97
AGACG 1545885 1.3153625 7.7250113 15-19
CGCGA 1261530 1.3062932 8.49213 20-24
GTCGC 1469240 1.2830421 7.6073804 40-44
TGTTC 2735710 1.2661263 6.4030037 70-74
GGGGT 1762975 1.2641457 7.0379105 35-39
TGTGT 3013370 1.2637491 5.8603816 70-74
GTTAC 2252535 1.2361566 5.7267647 55-59
ATTGG 2463500 1.2250558 32.18252 6
TGCGA 1706450 1.2245227 6.160633 50-54
TAGGA 2074545 1.2232668 7.89065 40-44
CGAAA 1578290 1.2178077 6.9583807 20-24
GGGTT 2215480 1.2149194 5.2391567 55-59
CCATT 2004605 1.2140298 7.1983924 55-59
CGGAT 1690605 1.2131525 6.2110963 85-89
ACGCG 1169330 1.2108217 9.895381 15-19
CATTC 1991130 1.2058692 6.740267 55-59
CGACT 1522315 1.2055254 6.9654183 50-54
CGGTA 1676875 1.2033001 9.310326 25-29
TTATG 3137625 1.1932546 8.562692 95-97
TTACC 1952885 1.1827072 5.114206 60-64
TAGAA 2167875 1.1591944 10.790021 8
GCCTA 1455380 1.1525193 6.8465667 30-34
CGCTT 1724675 1.1518188 6.681565 40-44
ATTGT 2980010 1.1333127 5.263518 65-69
AATTG 2431425 1.0964477 28.818167 5
GTTCG 1776255 1.0749383 5.9045815 90-94
TCGTG 1709370 1.0344615 7.347711 8
TGGCC 1122350 0.9801137 8.179225 1
TTAGA 2151550 0.97023845 9.852276 7
CCTAT 1527860 0.9253032 5.92163 30-34
CACAG 838845 0.7876776 6.0491376 1
CTACA 1029505 0.7393056 5.836872 3
GTCGT 1201215 0.7269407 7.144431 7
TGACT 1265380 0.6944211 7.895875 1
Produced by FastQC (version 0.10.1)