FastQCFastQC Report
Wed 6 Aug 2014
20L22C_TGACCA_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20L22C_TGACCA_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13871643
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 230399 1.6609351898690012 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 159382 1.148977089447876 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 146138 1.0535017373212388 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 118069 0.8511536809302257 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 115340 0.83148045260392 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 104411 0.7526938229307083 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 100139 0.7218971826192471 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 92203 0.6646869444376561 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 90062 0.6492525795250065 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 75616 0.545112067835079 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 60737 0.43785008019597965 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 58499 0.4217164469991046 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 57505 0.4145507493236382 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 55827 0.4024541289016737 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 52683 0.3797891857511039 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 49162 0.3544064679288531 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 46305 0.333810493825425 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 40933 0.29508400699181775 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 40112 0.2891654579057434 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 38186 0.27528101754060424 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 37885 0.27311112317408975 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 36671 0.26435945619419415 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 35861 0.2585202055733412 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 35276 0.25430296901383637 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 32270 0.23263286115422663 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 32123 0.2315731453008126 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 32035 0.23093875757904092 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 29552 0.21303893129314241 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 27184 0.19596813441637734 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 26126 0.18834106385234972 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 25883 0.18658928866609387 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 24435 0.1761507270623963 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 23910 0.17236602758591754 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 23083 0.16640422479154054 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 22662 0.1633692562589738 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 21254 0.153219052710627 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 21131 0.15233235169042342 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 20790 0.14987409926855816 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 20590 0.14843230899180435 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 20415 0.1471707424996448 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 20308 0.14639938470158148 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 19970 0.14396275913386758 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 19944 0.14377532639788956 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 19175 0.13823164278377117 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 19099 0.13768376247860473 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 18769 0.13530480852196095 No Hit
CGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTC 18725 0.13498761466107512 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 18611 0.13416579420332544 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 17757 0.1280093497215867 No Hit
CTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCC 17412 0.12552226149418638 No Hit
GCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGA 17340 0.12500321699455502 No Hit
TGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCT 17286 0.12461393361983147 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 17238 0.12426790395341056 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 16925 0.12201150217029084 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 16842 0.12141315920543803 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 16625 0.11984881675516014 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 16581 0.11953162289427431 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 15591 0.11239476102434297 No Hit
GTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCC 15579 0.11230825360773775 No Hit
ATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCA 15578 0.11230104465635396 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 15199 0.1095688520819055 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 15185 0.10946792676253274 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 14597 0.10522906334887655 No Hit
ATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGGAT 14574 0.10506325746704986 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 14333 0.10332590018356154 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 14329 0.10329706437802647 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 14085 0.10153808024038681 No Hit
ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC 13903 0.10022605108854085 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 6063495 4.421134 7.2895007 45-49
ACTTC 5210405 3.8381898 7.3695045 60-64
CTGCT 4720790 3.7392635 11.786877 5
AACTT 5895865 3.541526 7.188054 55-59
AGCTG 3940650 3.3391316 10.2490835 3
TCAAG 3960865 3.1213272 5.536389 60-64
CTTCA 4204150 3.0969424 7.8515816 60-64
CTACT 4176610 3.0766556 12.245532 2
TGCTA 4731645 3.0561275 5.5931163 95-97
CAACT 3384610 3.0419598 13.173568 9
CTGGT 4361915 3.0293741 17.404545 1
TTCAA 5013010 3.0112128 10.764729 9
GGAAG 3293800 2.9857757 6.166271 15-19
TCTCT 4824545 2.9128718 9.472557 20-24
ACCCA 2091030 2.8119469 35.77605 1
TCAAC 3092205 2.7791574 12.212424 8
CGGTG 3021210 2.752726 16.170225 9
AGCAC 2332150 2.7498415 5.5968146 90-94
AGGAA 3246065 2.736533 6.955573 40-44
TATGC 4218380 2.7246141 8.411119 30-34
GGTGT 4470765 2.7224631 13.831315 1
ACAAC 2470705 2.709291 7.28635 85-89
TGGTG 4390350 2.6734943 10.682041 6
GGTGG 3322760 2.6545167 11.969142 7
GCTGC 2515635 2.614119 9.076948 3
GAAGA 3100305 2.613653 5.2708397 90-94
ATGCA 3310575 2.6088715 6.832716 1
GAAAC 2681530 2.5782309 6.1169887 75-79
CAAGC 2175880 2.5655832 7.735168 75-79
GGTTC 3687890 2.5612602 5.206971 3
CTTCT 4222955 2.5496552 6.208533 40-44
CAACA 2312060 2.5353262 7.0897865 85-89
CCGTG 2420500 2.5152597 7.2346554 60-64
AAGCT 3178820 2.505043 5.37953 15-19
ACTGC 2537455 2.4522214 12.361361 4
CATGG 2880835 2.4410915 12.192275 2
CGTGA 2872925 2.4343889 10.309533 9
CTGAA 3065770 2.4159548 5.9093466 80-84
CCAAG 2045630 2.4120054 8.29931 25-29
GTTCC 3041935 2.4094687 5.6808968 45-49
GAAGC 2324870 2.403556 7.088971 15-19
TCCTT 3880815 2.3430846 8.072112 40-44
ACATC 2598390 2.335335 7.076291 40-44
GAGAA 2757245 2.3244426 5.782571 80-84
TCTGC 2923875 2.3159552 6.5603766 85-89
GCTGG 2531535 2.3065667 11.546552 4
GCAGC 1818220 2.3052287 6.5434875 1
CAAGG 2220970 2.2961395 6.7844286 10-14
ATGGT 4037015 2.2862518 6.4417286 1
GGCTC 2198390 2.2844543 6.8407927 10-14
CAGCA 1926360 2.271374 6.128599 35-39
ACTGA 2872890 2.2639573 5.3794274 70-74
CCTTA 3047715 2.2450674 8.922533 45-49
CTCTC 2445335 2.209049 6.5015016 15-19
CTCTG 2781960 2.2035465 7.908641 45-49
CTGCA 2257960 2.1821146 5.4202514 95-97
GCTAC 2253525 2.1778286 15.532426 1
CAAGT 2761120 2.1758778 5.286131 50-54
ATCCG 2225510 2.1507547 6.076524 65-69
TGGCT 3064260 2.1281455 9.14946 9
CAGCT 2175320 2.1022506 6.224286 65-69
TATTC 4267425 2.1009653 7.9932885 5
CTTGG 3016505 2.0949795 7.4715004 2
CTCTA 2843625 2.094726 7.5494466 95-97
CGGAA 2026110 2.0946841 6.036923 95-97
GTGGC 2289200 2.085767 11.265263 8
TAGCT 3229115 2.085657 7.362767 2
TTCCA 2808060 2.0685275 6.4188175 25-29
CACAA 1857110 2.0364435 7.508512 35-39
AAGGA 2404750 2.0272787 6.0057344 10-14
CATCC 1818015 2.0038 7.156212 40-44
TTCGG 2879185 1.99961 12.2492075 7
TTTCA 4043445 1.9906939 8.973527 8
GCATG 2315575 1.9621153 6.2929726 3
AAACT 2671100 1.9575951 5.2227507 65-69
TACTG 3027285 1.9552966 8.51835 3
CACCC 1181170 1.9479208 10.31359 50-54
TCCGT 2454235 1.9439609 5.040669 70-74
TCGGT 2795225 1.9412993 13.420502 8
AGAGC 1860180 1.9231385 6.521174 95-97
GGTAT 3395235 1.9227976 5.3588095 30-34
GCTCT 2414470 1.9124634 6.336196 20-24
GTGTA 3372935 1.9101684 12.572943 2
GCTCC 1610840 1.909087 5.8370233 65-69
GCAGG 1701955 1.8919953 7.10294 90-94
AATGA 2938980 1.888573 6.4876986 75-79
CTCCT 2084700 1.8832611 7.158176 70-74
GCACC 1298995 1.8783249 9.311395 50-54
CCCAA 1395215 1.8762382 35.929256 2
TGCTG 2696700 1.8728732 9.290177 6
GTGAA 2678240 1.8505594 5.342587 65-69
TTCCT 3061920 1.8486677 5.1665864 45-49
CCAGC 1274845 1.8434045 6.825644 1
CCAAT 2047625 1.8403281 24.158064 3
GAGCA 1770015 1.8299217 5.372294 90-94
GATCG 2154250 1.8254156 5.421139 95-97
CTATG 2814410 1.8178025 5.53119 35-39
CCACA 1343990 1.8073525 8.741526 60-64
CAGTG 2127715 1.8029311 6.584989 30-34
GTAGC 2125580 1.801122 8.995911 1
CTGTT 3397090 1.7983593 7.2866836 8
CTTTC 2977860 1.7979156 10.627644 7
GCTTC 2267015 1.7956668 7.499077 15-19
TGTAG 3136220 1.7761114 7.180694 3
AGCTC 1837155 1.7754446 5.0795994 65-69
TTGGT 3809720 1.7683443 14.374953 7
CGCAG 1385665 1.7568144 8.183914 90-94
CCTGT 2217180 1.7561935 8.387623 1
ACACA 1582575 1.7353978 7.380847 35-39
ACTAC 1922595 1.727956 6.10743 70-74
AAGTT 3277395 1.72614 5.3832216 60-64
CTGAG 2036840 1.7259276 6.4986806 80-84
GCACA 1462455 1.7243829 5.9228992 95-97
CTATT 3499300 1.7227973 5.116983 25-29
TGGTA 3030380 1.7161719 12.336556 2
AGTGC 2020215 1.7118404 5.762205 25-29
GAACA 1767185 1.6991086 5.9137607 80-84
ACGGT 2003430 1.6976175 7.7935104 4
AATCC 1888745 1.6975327 6.017637 60-64
AACAC 1541595 1.6904606 7.115091 35-39
TGAAC 2133150 1.6810112 5.5705442 80-84
TCCAA 1846725 1.6597667 6.637358 25-29
GCTTG 2381895 1.6542393 7.1048446 1
GCTGA 1943890 1.6471659 5.9379497 30-34
ATGAT 3124120 1.645413 5.54082 15-19
CATGA 2066290 1.6283228 5.4855037 15-19
ACCAG 1379790 1.6269126 5.248031 2
GGTAC 1915675 1.6232578 15.605503 3
ATGCC 1670605 1.6144891 8.04529 9
AGATC 2026970 1.5973369 5.1067514 95-97
TACTT 3215265 1.5829594 9.265121 5
CTACC 1422770 1.5681645 5.8193192 20-24
CACAC 1164670 1.5662091 6.6839986 95-97
GCTGT 2249395 1.5622174 9.490838 7
GTCTG 2245130 1.5592552 6.875898 90-94
GACGG 1388775 1.5438458 10.047142 3
ATTCC 2081420 1.533256 5.0724072 55-59
CTGTA 2373800 1.5332165 5.857612 8
AACAT 2088385 1.5305351 5.765401 40-44
AACGC 1280455 1.5097864 7.571188 95-97
TGCAC 1555600 1.5033469 6.430013 50-54
GGTCA 1756195 1.4881216 22.127419 9
GTATT 3442485 1.4860362 7.4086833 4
ACGCA 1249800 1.4736412 5.6472483 90-94
ACATG 1868335 1.472326 10.981629 1
AACCA 1337590 1.4667556 5.0339427 75-79
CTGAC 1514295 1.4634295 8.605545 1
AACTA 1995085 1.4621576 5.5918913 75-79
CCGCC 822055 1.457727 7.0181656 45-49
ATCTC 1960965 1.4445242 5.251869 45-49
ACTTT 2825045 1.3908439 9.125549 6
GCTAA 1761180 1.3878833 5.0916 85-89
GGCTG 1522735 1.387415 5.5957546 9
TGGTC 1990100 1.3821354 18.354795 8
CAGGT 1625810 1.3776392 6.4054437 95-97
ATTCG 2132710 1.3774984 10.185373 6
GCGGC 992175 1.3526094 7.377322 7
TGTAT 3089380 1.3336093 7.211978 3
GTACT 2048670 1.3232179 11.775899 4
AGCCT 1333865 1.2890602 6.2634764 2
AGGTA 1865135 1.2887356 5.500975 6
TGTTT 3639175 1.2875684 5.418839 9
GTATG 2257630 1.2785463 5.3174653 7
TGACG 1499415 1.2705376 7.519117 2
ATGGG 1707545 1.268651 10.789102 3
GCCTG 1217925 1.2656053 7.166754 3
CACAT 1405295 1.2630261 5.8578525 60-64
CTAAC 1381090 1.2412717 6.042298 95-97
CAATT 2053565 1.2335347 16.252981 4
GGTCG 1338480 1.2195343 10.529844 6
ACACG 988720 1.1658013 5.6599054 95-97
TGGGT 1869515 1.1384372 7.880571 4
CCATT 1542520 1.136281 5.4252224 55-59
AAGCC 962760 1.1351917 6.3238173 1
ATTGG 2003355 1.1345447 15.183418 6
GCTCA 1142385 1.104012 5.900382 1
CGGTA 1296350 1.0984694 7.2024136 5
GGGTC 1194350 1.0882125 10.528466 5
TGGCC 1021930 1.0619372 10.532838 1
TGCGG 1135020 1.0341549 5.2468133 6
GCGAC 815210 1.0335634 5.2482395 20-24
TCGTG 1430795 0.9936951 8.031696 8
TAACG 1248695 0.98402375 5.0182166 90-94
CGGCT 946420 0.9834712 5.4485173 8
TTGGC 1401930 0.9736481 5.035268 3
AATTG 1825520 0.96146584 13.330833 5
TGGGG 1184425 0.9462241 5.537269 8
GGGGT 1042570 0.8328977 5.2150598 9
GGCCT 748925 0.7782445 7.009586 5
GTCGT 1038250 0.7210704 7.796358 7
CCCTA 649355 0.7157133 5.045708 2
GCCTA 738375 0.7135728 6.2087893 3
GGCCC 435155 0.67658705 5.1685543 1
GCCCT 547915 0.64936143 5.906958 1
GGTAA 824870 0.5699531 5.913307 7
Produced by FastQC (version 0.10.1)