FastQCFastQC Report
Thu 31 Jul 2014
20L22B_TGACCA_L003_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20L22B_TGACCA_L003_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13007309
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 149486 1.1492461661362854 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 146464 1.12601307464903 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 142748 1.0974445213840924 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 89515 0.6881900014830123 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 88041 0.6768579111943908 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 78740 0.6053519601940724 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 72811 0.5597698955256618 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 66855 0.5139802552549494 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 66404 0.510512973897983 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 51564 0.39642327248472375 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 50482 0.38810487242211283 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 50117 0.38529875779840395 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 50054 0.3848144147263665 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 49719 0.3822389396607707 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 43147 0.3317135004634702 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 41989 0.32281081352030616 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 41601 0.31982787523537726 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 40338 0.3101179498388175 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 39907 0.30680442818725995 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 37769 0.2903675156790694 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 34627 0.2662118659593618 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 34473 0.26502791622771477 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 31243 0.24019572380420884 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 29757 0.22877137769234204 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 29646 0.22791801132732376 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 29594 0.22751823609326113 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 26130 0.20088705511647334 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 25761 0.19805018855168274 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25724 0.19776573309667664 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 23194 0.17831513036247545 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 22974 0.17662377360297968 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 22797 0.17526300021011265 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 22380 0.17205711035234114 No Hit
GCTGCTGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 21754 0.16724443157304866 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 21706 0.16687540828006778 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 20673 0.15893371949570814 No Hit
GCAGATGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 19489 0.1498311449355128 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 19016 0.1461947279025969 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 18929 0.14552587318406907 No Hit
GCAGGAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 18889 0.14521835377325165 No Hit
TGACGAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 18018 0.13852211860270253 No Hit
GCAGTAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 17969 0.1381454073244512 No Hit
TGGCTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 17722 0.13624647496265369 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 17489 0.13445517439464227 No Hit
TGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 17285 0.13288682539947347 No Hit
TTGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 17172 0.1320180830639143 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 16886 0.1298193192765698 No Hit
AGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 16860 0.1296194316595385 No Hit
CGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 16452 0.12648273366920093 No Hit
CTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGA 16282 0.12517577617322692 No Hit
GCAGAGGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 15934 0.12250035729911544 No Hit
GGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15723 0.1208781924070536 No Hit
GCTGTGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 15175 0.11666517647885508 No Hit
TAGATGAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 15076 0.115904065937082 No Hit
GCTGCCGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 14340 0.11024570877804164 No Hit
GCAGCAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 14340 0.11024570877804164 No Hit
GACCACACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 13988 0.10753953796284843 No Hit
CTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCA 13767 0.10584049321808223 No Hit
GTGTCAAACTCAAATTTAATTTCTTTCCAAACTTCACAAGCAGCAGCTAA 13346 0.10260385141922898 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AGCAG 6073665 5.333351 61.83986 20-24
TTTTT 8113215 5.103204 9.771001 7
TAGCA 6704905 4.6814194 38.543133 20-24
CACGG 3948755 4.6798744 44.968204 45-49
ACTTG 5425735 4.364436 101.67704 8
GCAGA 4917545 4.31815 39.20215 25-29
GTTTC 4546560 4.213444 40.516884 40-44
CCTGC 3176135 4.039551 49.253185 15-19
TCACG 4172430 3.931865 35.858368 45-49
CTGCG 2784470 3.801905 51.919342 15-19
CGGAT 3743410 3.787054 35.273857 45-49
GAAGT 4945415 3.706911 37.624638 7
AGAGA 5557565 3.6158404 22.685232 90-94
GAACC 4338890 3.548979 30.996323 70-74
CTTGA 4228120 3.4010804 31.164291 55-59
ACGGA 3821415 3.3556259 32.782158 45-49
AGTAG 4452795 3.3376603 37.464207 9
GCAGC 2782690 3.2979105 23.832895 20-24
AACTT 5703295 3.1662574 70.35627 7
CTTGT 3378460 3.1309278 117.32951 9
GCGTT 2673310 3.1157897 40.9496 15-19
TTCTC 3542875 3.0583384 31.158247 30-34
ATAGC 4355180 3.0408223 26.276787 80-84
TGCGT 2604015 3.0350251 43.913097 15-19
CCATG 3129385 2.9489574 36.059536 75-79
AGGAG 3127715 2.9484997 25.277666 90-94
AACCA 5164535 2.9154582 20.152937 70-74
ACCAT 4477260 2.9118743 33.014748 75-79
ACCTG 3066260 2.889472 27.80098 15-19
AGAGC 3238865 2.8440826 53.10349 95-97
CATGC 3017530 2.8435516 35.7342 75-79
GAGCC 2391410 2.834184 64.14117 95-97
TTCAC 3735700 2.7990909 23.092997 45-49
TGCAT 3425760 2.755666 28.561953 75-79
AGAAG 4234700 2.7551634 22.405487 6
CACTG 2854515 2.6899352 34.885323 85-89
ACAGG 3063200 2.689829 16.157543 60-64
AAGTT 4510125 2.6880264 23.5966 60-64
GCTGC 1966235 2.68469 41.607986 1
TGAAG 3572535 2.677848 21.819519 60-64
TTGAA 4437940 2.645004 21.387325 55-59
CTCAG 2803420 2.6417863 35.696575 35-39
TTTCA 4109280 2.6282752 23.257128 40-44
GAGAG 2771685 2.61287 42.939632 95-97
GCACT 2765225 2.6057935 26.423494 85-89
AGCAC 3178880 2.6001532 28.430916 80-84
GTTGT 2560510 2.5474482 18.065798 35-39
GACTC 2700240 2.5445552 35.42396 25-29
GATGA 3368250 2.524723 12.849532 15-19
GGATT 2899940 2.5042818 24.821905 50-54
GGAAC 2822440 2.4784153 33.27287 70-74
TTGTA 3569455 2.450934 32.47846 9
GTAGT 2794675 2.4133785 24.817406 40-44
GGAGG 1747010 2.386264 21.652515 90-94
GGCTC 1736950 2.371625 24.88582 65-69
TGTTG 2381345 2.3691971 16.465542 35-39
AAACT 4899855 2.3611245 34.156002 6
TTAGC 2934325 2.3603575 30.782686 20-24
AGTTT 3410105 2.3415174 26.487003 40-44
CATTC 3099785 2.3226118 28.725061 30-34
GTTAC 2886835 2.3221571 28.076506 60-64
ACCAG 2824035 2.309909 15.81747 55-59
ATGCA 3303875 2.3067925 26.62808 75-79
CTGAA 3292410 2.298787 25.863731 85-89
TTTCC 2662425 2.2983022 15.194813 40-44
CAGTA 3262495 2.2779005 26.39398 35-39
TCTCA 3036330 2.275066 21.771957 35-39
CAGAC 2774795 2.2696333 30.83384 25-29
CAGCA 2768085 2.264145 19.676252 20-24
TCAGT 2811225 2.2613368 30.589327 35-39
GTAGG 2070150 2.248332 28.337225 7
CTCAT 2980455 2.2331998 21.68565 30-34
GTTAG 2582200 2.2298932 32.876484 20-24
CAGGC 1872295 2.2189538 18.987736 65-69
GCATA 3171870 2.2146251 20.150915 80-84
AAGGA 3394985 2.2088315 18.869097 70-74
CAGAA 3617745 2.1924925 16.021933 5
CATAG 3124975 2.1818829 26.378872 80-84
GAAGA 3326390 2.1642025 11.060753 50-54
AGGAA 3313010 2.1554973 24.789593 70-74
TAGAG 2870025 2.151271 27.307978 90-94
CTGCA 2277545 2.146231 22.40538 3
TAACC 3288420 2.1386888 24.540716 10-14
CCATC 2422450 2.1263745 15.745406 75-79
TGTAA 3560250 2.121903 23.010809 10-14
CGTTA 2624880 2.1114416 22.95158 20-24
CCAGA 2566015 2.0988624 20.363539 55-59
TCATT 3262920 2.0869474 24.44864 30-34
AACCT 3199425 2.080809 23.135979 10-14
GGAGC 1630290 2.074261 23.033276 95-97
TAAGG 2765860 2.0731926 26.177513 65-69
ATTCT 3219705 2.0593073 24.583858 30-34
TGAAT 3446610 2.0541735 21.15625 85-89
CTAAG 2929680 2.0455263 26.183891 65-69
TTCCG 1872575 2.0329816 19.25746 40-44
GTAAC 2909265 2.0312724 26.610104 10-14
TTGTT 2563575 2.0279648 15.087418 35-39
GGATG 1864740 2.025242 21.210577 10-14
TAGTT 2945775 2.0226898 26.037678 40-44
AGGGA 2125625 2.0038283 24.684856 9
ATGAT 3356135 2.0002506 11.275377 15-19
TGATG 2296510 1.9831816 16.167686 30-34
GATGT 2287710 1.9755822 20.083067 30-34
AGACT 2822585 1.9707516 26.556856 25-29
ACTGA 2806310 1.9593883 26.06884 85-89
TAGGG 1797615 1.9523396 28.258476 8
TGCAG 1928345 1.9508274 25.85614 4
GAATA 3764485 1.9474478 14.880635 90-94
AGTTA 3256395 1.9408054 22.356192 60-64
AAGAG 2966830 1.930267 12.183281 50-54
TGTTT 2409820 1.906334 18.685772 35-39
TCCGT 1750425 1.9003681 19.84504 40-44
ACTCA 2921510 1.9000617 22.958332 25-29
AATAG 3630700 1.8782381 19.706781 90-94
TTACT 2930600 1.8743973 18.33865 65-69
CAGAG 2129780 1.870183 15.893129 25-29
TTAAA 4495710 1.8492433 16.012276 50-54
AGGCT 1814960 1.8361202 17.950487 65-69
CAGGA 2081400 1.8276999 14.911312 90-94
GCTCA 1904120 1.7943362 21.785189 65-69
GGGAT 1641035 1.7822822 25.000998 10-14
CCAAA 3101230 1.7506913 26.492353 4
GAGAT 2331620 1.7477013 17.523033 25-29
ATTAA 4232630 1.7410294 16.045752 50-54
TCCTC 1713295 1.7326139 8.116581 1
TAAAC 3568245 1.7194532 17.299608 50-54
GAGGA 1820260 1.7159606 21.588718 95-97
CATCA 2623965 1.7065475 10.463314 80-84
CTACA 2613700 1.6998714 12.429893 85-89
TACTA 3050775 1.693677 21.01516 65-69
CTCAC 1927000 1.6914791 15.858913 65-69
AAGAA 3747265 1.6826351 11.329867 45-49
GATTA 2821645 1.6816952 22.43626 50-54
AGCTG 1658625 1.6779627 9.755912 95-97
TACAG 2399530 1.6753712 11.953236 90-94
CCTCC 1407275 1.6672032 5.883408 1
GAGCT 1618870 1.6377441 11.643261 95-97
TACCA 2516980 1.6369677 11.818809 75-79
ATACC 2493620 1.621775 11.38198 75-79
TGACC 1717890 1.6188434 43.897293 1
GGAAG 1709660 1.611698 12.263034 6
ACCAA 2846470 1.6068754 13.951424 3
AACAG 2633335 1.5959021 16.228146 60-64
ATGTT 2285455 1.5692871 12.543769 30-34
GAAAC 2587020 1.5678334 10.974087 60-64
ATAGA 3000465 1.5522041 19.409836 90-94
GAGAA 2372260 1.5434302 15.410888 50-54
TAAAG 2963095 1.5328718 10.254544 45-49
CCGTA 1625880 1.5321383 21.633495 40-44
ACTAA 3167420 1.526305 18.44747 65-69
TCTAC 2030720 1.5215812 16.123058 85-89
AGATG 2013305 1.5091034 13.658082 25-29
GTAAA 2843030 1.4707596 9.098641 45-49
TGCTG 1245925 1.4521474 7.9985437 1
AGAAA 3212075 1.4423186 7.772898 60-64
AAAGA 3177325 1.4267148 8.905383 45-49
AAGTA 2751445 1.4233807 26.9823 8
GATAC 2034920 1.4207976 10.733808 75-79
AGCCA 1709505 1.3982832 13.262369 95-97
TCTTG 1500825 1.3908631 5.0184503 4
CTGCT 1271535 1.3804559 5.718343 2
AGAAC 2262450 1.3711315 12.29386 50-54
CAAAC 2397640 1.3535041 31.212088 5
GACCA 1645140 1.3456361 17.905203 2
GCTCC 1055980 1.3430427 5.47142 1
CAATA 2772055 1.3357879 10.726503 80-84
GAGCG 1048050 1.3334616 6.756322 90-94
CTCTT 1540180 1.3295394 5.554417 3
GATCG 1308465 1.3237201 5.2182255 95-97
GATAG 1763535 1.3218845 17.336905 15-19
GGGGG 666510 1.3191038 6.59273 1
TCAAT 2367735 1.3144785 9.720119 80-84
CGTAA 1856350 1.2961185 12.617941 40-44
TGATA 2154745 1.284224 11.229707 15-19
GCCAA 1536000 1.2563655 13.337462 3
AAACA 3003450 1.2562363 8.1015415 60-64
GGATA 1655960 1.2412502 13.170749 70-74
TATCT 1927330 1.2327107 10.749769 85-89
ATCAA 2556830 1.232076 8.478074 80-84
AGCGT 1215590 1.2297623 5.1673665 90-94
GGCCA 1030970 1.221856 15.920157 2
CGGAA 1366445 1.1998903 10.097539 5
CAACA 2122110 1.1979632 6.2592273 8
CCTCT 1181710 1.1950349 6.399998 2
ATCTA 2094425 1.1627469 9.711005 85-89
GCTGG 789130 1.1567295 6.9196687 1
TGTAG 1315590 1.1360952 6.2205544 3
GCCCA 1026630 1.1333483 6.660338 2
TGCCA 1201755 1.1324667 5.4460716 1
CTTTT 1521075 1.1208318 5.1240497 6
TGGCC 806675 1.101431 30.09474 1
AATAT 2638345 1.085244 7.143032 80-84
CCCAA 1412855 1.0764596 8.285373 4
CCAAC 1411095 1.0751189 6.1759367 5
CCGAA 1278255 1.0455439 27.921219 4
TCAAC 1590225 1.0342342 7.3030276 7
ATATC 1860645 1.032961 8.493623 85-89
GTTGC 858140 1.0001771 6.9970994 1
CCATA 1521540 0.9895635 5.5253906 3
TGTCC 910980 0.9890154 9.3835945 1
GTCCA 1009705 0.9514895 5.1285086 2
CTAAA 1972875 0.9506819 5.5978484 4
GGTGC 646310 0.9473798 10.629271 2
CTCCG 737955 0.93856424 5.3606825 2
CAACT 1431765 0.93117654 5.4206305 6
GTGCA 919000 0.92971444 8.255189 3
GGCTG 634205 0.929636 10.229446 1
GAACT 1308495 0.91360176 25.067493 6
CCGTT 826355 0.89714134 5.58942 4
GCTGT 769470 0.89683074 10.419877 1
GACCT 941400 0.8871227 9.994145 2
CCTAA 1361215 0.88529295 10.373528 4
GGGTG 559935 0.88114107 12.35996 1
CTGTC 802610 0.8713624 9.526715 4
ACCTA 1331280 0.86582416 7.037995 3
TGCCC 676190 0.8600087 8.157456 1
ACCCA 1105425 0.8422277 5.1792555 3
TGTCA 1028605 0.82740515 7.5739307 5
CGAAC 1009380 0.8256186 26.372454 5
ACCGA 986155 0.80662185 16.433043 3
GCAGG 632310 0.80450475 6.338312 1
GGCCT 588205 0.8031329 7.2871375 2
CCGTC 608920 0.77445173 5.8440003 4
AAGTC 1096650 0.7656899 5.517612 6
TGGCT 654745 0.7631167 7.382183 1
GTCAA 1052420 0.73480815 7.4557014 6
GCCAG 617115 0.731375 5.1499457 2
AGTCT 903815 0.7270246 7.9908624 7
TGCGG 493710 0.7236944 15.996204 3
GGGCC 418450 0.7185662 9.036119 1
GTAGA 941400 0.7056407 5.4273424 4
CGACC 617120 0.6812697 5.173873 1
CCCCC 490780 0.68113995 5.0521197 1
TCTAA 1204945 0.6689407 5.849352 9
GGCCG 380700 0.6537415 16.939949 2
TGACT 793985 0.6386779 12.488846 1
GACTG 622680 0.62993973 8.672839 2
GACCG 524220 0.6212803 23.042803 2
GCCGA 517085 0.61282426 12.422681 3
CTGTA 755490 0.60771275 5.496971 2
TAACT 1086270 0.6030568 8.752845 6
CTAAC 926485 0.6025578 9.520876 5
GACTT 744420 0.59880805 8.653981 6
TCCCG 464280 0.5904921 5.007129 2
GTCTA 727955 0.5855637 8.01693 8
GCCCC 391195 0.5828642 5.9911885 1
GGTAG 529885 0.5754933 5.267734 6
CCCCG 378660 0.5641876 5.7198067 2
GGCCC 348935 0.55814004 6.476885 1
GCGGA 438580 0.55801696 12.988567 4
GACCC 497830 0.54957944 7.1316385 2
GCCCG 313395 0.50129193 6.297284 2
CCCGA 453305 0.500426 5.478347 3
GTCCG 360640 0.4924165 6.375666 2
CCCGT 383025 0.4871483 5.930204 3
Produced by FastQC (version 0.10.1)