FastQCFastQC Report
Thu 31 Jul 2014
20L22B_TGACCA_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20L22B_TGACCA_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13007309
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 167101 1.2846700266750026 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 155960 1.1990181827770832 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 143488 1.1031336304842148 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 107270 0.8246901799595905 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 84317 0.6482278540472899 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 80462 0.6185906708297619 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 69120 0.5313935418924852 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 68460 0.526319471613998 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 67676 0.5202920911619767 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 67155 0.5162866508360799 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 64975 0.49952684294653105 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 52985 0.4073478995540123 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 51846 0.3985912843309865 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 50833 0.3908033552520356 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 45967 0.35339361892609766 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 44700 0.34365294158845616 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 42989 0.33049879879074145 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 41717 0.32071968152674774 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 41221 0.3169064408326119 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 41056 0.31563792326299006 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 39390 0.3028297398024449 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 35151 0.27024037024106984 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 32302 0.24833730020559977 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 31999 0.2460078406686579 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 30347 0.23330728900189884 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 29048 0.22332059613560346 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 28537 0.21939203566241103 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 28266 0.21730859165412308 No Hit
TAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTA 26982 0.20743721856688419 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 26902 0.20682217974524936 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 26865 0.20653772429024328 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 26637 0.20478486364858403 No Hit
CGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTC 26397 0.20293974718367958 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 26218 0.20156359782027167 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 24918 0.19156921696870582 No Hit
GGTCCTTACGAGTTAATCGTTCTTCACTTCTTACTTGGTGTAGCTTGCTA 24386 0.18747920880483426 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 23699 0.18219756292404524 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 22706 0.17456339355050304 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 21852 0.16799785412955132 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 21135 0.16248556869064923 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 20892 0.16061738826993346 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 20562 0.15808035313068983 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 20038 0.15405184884898174 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 19938 0.15328305032193823 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 19285 0.148262795940344 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 19029 0.14629467171111257 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 18989 0.14598715230029516 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 18925 0.1454951212429873 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 18910 0.14537980146393079 No Hit
CGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTG 18859 0.14498771421513856 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 18429 0.14168188054885142 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 17720 0.13623109899211283 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 17675 0.13588513965494323 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 17548 0.13490876552559797 No Hit
TGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATT 17395 0.13373250377922136 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 17332 0.13324816070718393 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 17313 0.13310208898704567 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 16852 0.12955792777737501 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 16230 0.12477600093916427 No Hit
GCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTC 15869 0.12200063825653716 No Hit
GTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTGCT 15626 0.12013245783582138 No Hit
GGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGACTGCTACTT 14915 0.11466630030854191 No Hit
GGCCCTGGAGATTTCTTAGTTCATCACGCTATTGCTTTAGGTTTACATAC 14863 0.11426652507447928 No Hit
CGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGGATCGCTGT 14577 0.11206776128713479 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 14233 0.10942309435410508 No Hit
CGTGGTGGACTTGATTTCACTAAAGATGATGAAAACGTAAACTCTCAACC 13843 0.10642478009863532 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 13756 0.10575592538010743 No Hit
TATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 13200 0.10148140556974544 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
ACTTC 4500850 3.641849 6.5971975 40-44
CTGCT 4063755 3.4425988 16.353703 5
AACTT 4911270 3.2426975 5.1840386 55-59
AAAAA 3971070 3.2045844 6.7228365 45-49
CAACT 3173045 3.142221 7.0102587 9
CTACT 3835400 3.1034021 16.030378 2
ACTGC 2718705 2.8187342 17.71446 4
CTTCT 4245840 2.8071 9.095034 40-44
CTTCA 3376060 2.7317286 5.773667 60-64
AGCTG 3073090 2.7219746 8.351263 3
TTCAA 4087850 2.6990294 6.641668 55-59
AGGAA 3046065 2.6944265 5.974321 40-44
TCAAC 2680425 2.654387 6.4238906 55-59
ACCCA 1768100 2.6261241 29.307482 1
GCAGC 1975975 2.6250536 9.5152645 65-69
TCTCT 3953985 2.6141427 7.4767776 20-24
CTGGT 3557160 2.574418 10.947168 1
GCTGC 2338560 2.5384703 8.401335 3
CTGCA 2410835 2.4995368 7.7821617 35-39
GAAGC 2286020 2.478118 5.8140864 35-39
AGCAC 1941930 2.4641023 5.9558773 35-39
ACAAC 2027425 2.4571881 5.813648 85-89
GGTTC 3353715 2.4271789 11.661763 3
CAGCA 1905325 2.4176545 6.009922 35-39
CGGTG 2587545 2.3995414 16.129868 7
CAAGG 2178555 2.3616223 5.9661236 4
CTCCT 2343780 2.3241224 10.654723 70-74
CAAGC 1816825 2.3053575 6.4521265 1
GGTGT 3707320 2.2921999 12.935872 8
ATGGT 3876305 2.2891746 12.25145 1
TATGC 3306995 2.286008 6.080439 30-34
CAACA 1877470 2.2754464 5.735241 85-89
CAGCT 2176145 2.2562115 7.711018 65-69
GGTGG 2819675 2.233861 9.320656 7
TGCAG 2491600 2.2069228 7.879257 65-69
TGGTG 3566035 2.2048452 8.422315 6
CCGTG 2030440 2.204011 7.6872225 95-97
GCTCC 1729055 2.1969268 9.99493 70-74
CTCTG 2580605 2.1861522 7.7314177 45-49
GCTAC 2103845 2.1812518 20.64995 1
CCAAG 1688980 2.1431358 5.881281 75-79
TCTGC 2509645 2.1260388 5.2045107 85-89
CATGG 2368740 2.0981002 10.58877 2
GATGG 2752750 2.0830145 5.128335 1
ATGCA 2439175 2.063575 5.4575925 1
TGGTT 4264540 2.057785 8.079673 2
TTTCA 3805825 2.053186 5.2939224 8
CGTGA 2312385 2.048184 8.543617 9
AGCTC 1971685 2.044229 8.017383 70-74
CCCAA 1332040 1.9784526 29.972788 2
GCAAC 1546710 1.9626102 8.250993 35-39
ATCCG 1892380 1.9620064 9.59737 95-97
CCAGC 1260600 1.9602759 7.111516 30-34
CCTAC 1608495 1.9520681 7.5208106 20-24
TCCTT 2951330 1.951246 5.5911016 40-44
CTCTA 2382850 1.9280758 8.17325 25-29
GTTCC 2253535 1.9090759 5.1476665 95-97
CCTGT 2241220 1.8986434 9.177034 1
GCTTC 2233730 1.8922981 6.838423 15-19
GCTGG 2039055 1.890903 10.062336 4
CTTGG 2610865 1.8895572 7.2622075 2
CTTTC 2856705 1.8886855 6.1826296 7
CGGAA 1741130 1.88744 6.007647 95-97
TGCTG 2600920 1.8823597 12.485835 6
CCAAT 1895970 1.8775519 20.045755 3
AATGA 2711010 1.8715162 6.3902273 75-79
CCTCC 1246315 1.8536079 6.9160733 70-74
CTGTA 2665030 1.8422403 7.544951 8
CACAA 1495195 1.8121389 5.3782363 85-89
GCAGG 1590265 1.8048579 5.4293523 4
TTCCT 2716290 1.7958513 5.51772 20-24
TTCCA 2217085 1.7939476 5.4228997 95-97
GAAAA 2119920 1.7910807 5.8962507 40-44
TTCGG 2468445 1.7864839 11.400757 5
GGAGA 1921065 1.7791016 5.727658 7
TAGCT 2571865 1.7778387 5.7967386 2
CTGTT 3143645 1.7755976 10.002807 8
TCCGT 2083685 1.765188 7.354397 95-97
TGGTA 2984015 1.7622274 8.706333 2
CCTTA 2167760 1.7540364 5.3822255 95-97
TACTG 2523550 1.7444402 11.92039 3
CATCC 1426045 1.7306471 5.420815 90-94
ATGAT 3063160 1.7278223 5.8739796 15-19
TCGGT 2383585 1.7250684 13.075906 8
TGGCT 2376545 1.7199733 6.2960663 9
TGCAA 2020515 1.709383 6.3852205 35-39
ACCAG 1344220 1.7056721 6.5917897 30-34
GGCTC 1571090 1.7053936 5.3111367 1
CTATT 3149955 1.6993539 5.385886 25-29
CACCC 930140 1.6930568 5.5174646 50-54
ATTGC 2422900 1.6748644 5.269975 55-59
GCACC 1072200 1.6673075 5.075179 75-79
CTACC 1373530 1.6669149 7.228163 20-24
TGTAG 2815485 1.6627009 6.4165473 9
ATTCC 2046110 1.6556036 6.091608 20-24
TATTC 3068125 1.655208 6.2497215 5
CCGCC 863240 1.645077 9.526198 45-49
ATGCC 1577610 1.6356549 7.5817227 9
CTATG 2333190 1.6128511 5.749934 4
ACGGT 1814035 1.6067728 7.2280865 4
GCTGT 2190440 1.5852838 12.848577 7
TTCAT 2924575 1.5777647 6.0382943 60-64
TTGGT 3262095 1.5740712 10.573432 7
CACTG 1516815 1.5726233 5.469895 35-39
TACTT 2903975 1.5666513 5.695329 5
GTGGC 1687470 1.5648634 7.5387797 8
TTGCA 2251830 1.5566096 5.3874164 65-69
CGCAG 1157110 1.5372036 6.0236573 90-94
GACGG 1344255 1.5256509 9.513058 3
GGTAC 1702310 1.5078129 9.598091 3
AAACC 1230310 1.491105 5.5292797 40-44
GCTTG 2041235 1.4772998 6.9450407 1
TCCTG 1740290 1.4742818 6.600145 75-79
GCACT 1418955 1.4711624 6.2591248 35-39
ACACA 1204580 1.4599206 5.082475 85-89
GTAGC 1645115 1.4571527 7.1102777 1
GTGTT 2991590 1.4435434 8.126142 9
AAAAC 1449585 1.4335823 5.305983 40-44
TGTTT 3797430 1.4300555 7.4625344 9
CTGAC 1378735 1.4294627 8.079541 1
ACTCT 1758680 1.4230306 5.7588797 25-29
AGCCT 1369855 1.4202559 8.835473 2
GGTCA 1594495 1.4123162 16.098873 9
GGCTG 1518915 1.4085549 6.489616 9
GTGTA 2369875 1.3995435 9.611866 2
AACCA 1154365 1.3990614 5.09111 75-79
AACAC 1133735 1.3740584 5.0738935 85-89
ACTTT 2546670 1.3738906 5.5682416 6
TCTAT 2545880 1.3734643 5.298527 25-29
TCCTC 1372360 1.3608497 6.403684 70-74
GTATT 2942125 1.3559928 5.769051 4
GCGGC 970775 1.3502271 8.412691 7
CACAC 908170 1.348887 5.0568757 95-97
CAATT 2020255 1.3338863 13.250274 4
ATGGG 1756540 1.3291795 9.978741 3
ACATG 1565775 1.3246667 9.492855 1
TGGTC 1820940 1.3178662 13.322528 8
ACCGC 846920 1.3169895 5.6212344 45-49
CAGGA 1176655 1.2755312 5.13011 5
TGACG 1434050 1.270203 7.016241 2
GTACT 1832070 1.2664447 7.24036 4
AGGTA 1749855 1.2647249 6.3972974 6
GTATG 2140010 1.2637954 5.0067105 7
GCCTG 1160860 1.2600954 8.60339 6
AAGCC 990450 1.2567756 9.268819 1
TACCA 1261835 1.2495773 5.6250153 30-34
GGTCG 1331490 1.2347478 8.645479 6
TGTAT 2674315 1.2325621 5.616687 3
TACTC 1486880 1.2031044 5.407173 25-29
GCGAC 893945 1.1875927 6.8619323 20-24
GTAGA 1640995 1.1860454 5.8861403 4
TACCC 972075 1.1797094 5.5364275 20-24
TGGGT 1870970 1.1568028 6.412187 4
ATTGG 1950315 1.1517699 11.41238 6
TATGG 1937180 1.1440129 5.2540364 5
TCCTA 1384150 1.1199807 5.689912 20-24
GTTCG 1542315 1.1162173 11.254818 4
GGGTC 1190505 1.1040063 10.015144 9
CGGTA 1233845 1.0928723 6.6304326 5
CGCCT 846895 1.0760596 6.1177006 45-49
TGGCC 990995 1.0757096 9.594565 1
ATTCG 1512630 1.0456272 7.902479 6
CCAGG 779320 1.035315 5.400239 4
TGGGG 1289205 1.0213606 7.807601 7
TGCGG 1096125 1.0164837 5.9893084 6
TCGTG 1395705 1.0101115 6.63811 8
TTGGC 1395660 1.0100788 5.8940277 3
AATTG 1783355 1.0059286 10.308766 5
CGGCT 899780 0.9766971 6.3698025 8
CGACT 915075 0.9487432 5.2355976 20-24
GGGGT 1153805 0.9140911 7.6724234 8
GCCCA 575720 0.8952642 6.3881044 2
GTCGC 801835 0.8703793 5.2795105 10-14
GCCTA 785020 0.8139032 8.466736 3
GGCCC 486460 0.791988 8.737797 1
GGCCT 698805 0.7585419 8.432379 5
CCCTA 617975 0.74997395 6.229796 2
GCCCT 581825 0.7392633 7.0003514 1
GTCGT 962745 0.6967659 6.490417 7
CCTAT 835345 0.67591685 6.337477 4
GGTAA 850320 0.6145771 5.5929055 7
CCTAG 397425 0.41204745 5.1211514 3
Produced by FastQC (version 0.10.1)