FastQCFastQC Report
Thu 31 Jul 2014
20L22A_TGACCA_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20L22A_TGACCA_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 17203636
Filtered Sequences 0
Sequence length 101
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 296820 1.7253329470584011 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 285979 1.662317198527102 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 182909 1.063199663140978 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 174487 1.0142448956720544 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 151481 0.8805173510995001 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 116247 0.6757118088292497 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 98915 0.5749656642351652 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 92922 0.5401300050756712 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 91513 0.5319398759657552 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 89943 0.5228138981782688 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 84877 0.49336663482068555 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 75377 0.43814575011933526 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 70707 0.41100032574509254 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 67748 0.3938004733417982 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 64642 0.37574615040680936 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 61765 0.3590229414293583 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 58127 0.3378762489510938 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 57726 0.3355453463442263 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 55317 0.32154249252890493 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 53162 0.30901607078875654 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 51498 0.2993436968789621 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 48978 0.28469563062134073 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 48911 0.2843061780660786 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 45707 0.26568220810996 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 43664 0.253806811536817 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 43169 0.25092951280764136 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 41290 0.24000740308618482 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 40938 0.23796132398988212 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 40181 0.23356109138789033 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 38496 0.22376665025928238 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 38315 0.22271454708760402 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 37387 0.21732033856098792 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 34234 0.198992817564845 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 32658 0.18983196342912625 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 32258 0.18750687354696416 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 31343 0.1821882304415183 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 30459 0.17704978180194 No Hit
AGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 28391 0.16502906711116186 No Hit
GGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 27889 0.1621110793090484 No Hit
TGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 27721 0.1611345415585403 No Hit
TGGCTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 27399 0.15926284420339978 No Hit
TGACGAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 27136 0.1577340976058782 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 25702 0.149398650378327 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 25656 0.14913126504187835 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 24417 0.14192929913188118 No Hit
TTGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 24242 0.14091207230843528 No Hit
CTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCA 23697 0.13774413734398938 No Hit
GCAGGAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 21053 0.12237529322289777 No Hit
CGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 20921 0.12160801356178427 No Hit
CGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 20220 0.11753329354329514 No Hit
CTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGA 20115 0.1169229574492276 No Hit
GGCCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 19076 0.11088353648031149 No Hit
TGGTCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 19036 0.11065102749209528 No Hit
GCAGATGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 18714 0.10877933013695477 No Hit
GCTGCTGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 18550 0.1078260432852683 No Hit
GCAGTAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 18272 0.10621010581716563 No Hit
GACCACACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 17617 0.10240277113512516 No Hit
GCAGAGGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 17349 0.10084496091407655 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 20579150 8.827983 14.487532 7
AGCAG 8589300 5.894287 70.25686 20-24
CACGG 5734965 5.483881 60.352036 45-49
TAGCA 10136090 5.3490667 44.48431 20-24
ACTTG 8496725 5.101734 131.65704 8
CCTGC 4841970 4.961319 65.6336 15-19
GCAGA 7070560 4.8520727 46.918385 25-29
CTGCG 4358030 4.741396 68.8781 15-19
TCACG 6030625 4.4345913 46.40255 45-49
GTTTC 6462495 4.4149446 48.09363 40-44
CGGAT 5504975 4.2982097 45.909573 45-49
GAACC 6334000 4.093649 40.52335 70-74
GAAGT 7259265 4.0676274 35.42762 60-64
ACGGA 5619370 3.8562138 42.70848 45-49
AGAGA 7703770 3.793963 28.87248 90-94
GCGTT 4251180 3.7765958 52.55931 15-19
AGTAG 6632910 3.7166584 33.389153 35-39
CTTGA 6154135 3.6951604 38.296364 55-59
TGCGT 4146330 3.6834512 55.66591 15-19
AACTT 8888110 3.60704 89.099884 7
GAGCC 3654940 3.4949224 87.79775 95-97
ACCTG 4699870 3.456027 36.606976 15-19
CTTGT 5036910 3.4410365 149.88255 9
CCATG 4641650 3.4132152 46.308407 75-79
GCAGC 3541000 3.3859706 24.604424 20-24
TTCTC 5239935 3.3713975 38.66034 30-34
CATGC 4578505 3.3667817 46.203117 75-79
ATAGC 6265445 3.306431 32.995316 80-84
AACCA 7442360 3.2510111 26.021235 70-74
ACCAT 6504630 3.2328763 38.283592 75-79
CACTG 4347945 3.1972406 45.421627 85-89
AGAGC 4646775 3.1887846 68.63601 95-97
CTCAG 4323075 3.1789527 46.338917 35-39
GCACT 4239835 3.1177425 35.08796 85-89
GAGAG 4266160 3.1085055 56.829987 95-97
TGCAT 5169665 3.10405 35.642017 75-79
GACTC 4204595 3.0918288 45.915424 25-29
TTCAC 5447645 3.0805912 28.976868 45-49
AGCAC 4695895 3.0349457 37.72764 80-84
AGGAG 4114455 2.9979668 25.116661 90-94
GGAAC 4350190 2.9852571 43.224228 70-74
TGAAG 5308180 2.9743645 27.562891 60-64
AAGTT 6735020 2.9021606 28.182934 60-64
GGATT 4440210 2.8308103 31.17675 50-54
TTTCA 6117120 2.8245373 27.9422 40-44
AGAAG 5708920 2.8115366 21.399315 6
CAGAC 4338550 2.8039944 40.5637 25-29
TTGAA 6487545 2.7955222 25.828165 55-59
GTAGT 4317235 2.7524087 31.139433 40-44
TTAGC 4551035 2.7326026 38.21867 20-24
ACAGG 3978090 2.7299087 16.660175 90-94
GCTGC 2490375 2.709448 38.58463 1
GTTAC 4494495 2.698654 35.29358 60-64
TCAGT 4484300 2.6925325 38.182617 35-39
AAACT 7548110 2.6922848 45.524414 6
GTTAG 4194210 2.6739755 40.495712 20-24
TTGTA 5419960 2.6572802 35.84257 9
ATGCA 5035350 2.6572793 33.395832 75-79
CATTC 4687735 2.6508696 35.96575 30-34
CAGTA 4963890 2.6195679 33.12217 35-39
TCTCA 4623050 2.6142907 27.782736 35-39
CTGAA 4913985 2.5932317 32.494095 85-89
CTCAT 4540300 2.5674963 27.686579 30-34
TAACC 5147880 2.5585563 31.108297 10-14
AGTTT 5186840 2.542987 31.4022 40-44
AAGGA 5148825 2.5357003 24.19764 70-74
CATAG 4786860 2.526145 33.062595 80-84
CGTTA 4204710 2.5246568 29.151505 20-24
GCATA 4748995 2.5061624 25.705992 80-84
AGGAA 5076650 2.5001554 31.158375 70-74
TAGAG 4459830 2.4990034 34.116226 90-94
GGAGG 2289110 2.4677768 22.244709 90-94
GTAAC 4629360 2.4430282 33.436985 10-14
AACCT 4876435 2.4236448 29.66496 10-14
CTAAG 4584745 2.419484 32.920563 65-69
TAAGG 4311765 2.4160373 32.979637 65-69
TGTAA 5549380 2.391261 27.660503 10-14
GTTGT 3233675 2.3456457 16.545183 35-39
AGACT 4426920 2.3361955 33.398983 25-29
GGCTC 2136990 2.3249764 24.766884 65-69
GATGA 4137390 2.3183284 11.721427 15-19
ACTGA 4338550 2.2895606 33.001736 85-89
TCATT 4946425 2.283977 29.265387 30-34
ACCAG 3526505 2.2791715 15.562816 55-59
CAGGC 2377245 2.2731664 18.815731 65-69
TAGTT 4574705 2.2428715 30.974157 40-44
ATTCT 4838505 2.2341456 29.30148 30-34
ACTCA 4489275 2.2312217 29.467875 25-29
AGTTA 5108330 2.20121 26.896664 60-64
TGAAT 5076670 2.1875677 25.712729 85-89
CTGCA 2969355 2.183501 21.71085 3
CAGCA 3351205 2.1658757 18.91954 20-24
CAGAA 4629865 2.1474204 15.598617 5
GGAGC 2114915 2.1472924 24.202976 95-97
TGTTG 2958605 2.146115 14.758879 35-39
GTAGG 2575955 2.1355588 24.917381 7
TTACT 4572850 2.1114814 22.47346 65-69
CCATC 3029340 2.0979652 16.021164 75-79
GAATA 5481015 2.0757966 18.471851 90-94
TTTCC 3214795 2.0684135 13.742062 40-44
CCAGA 3181175 2.0559857 19.599823 55-59
GAAGA 4157580 2.0475307 10.30962 50-54
AATAG 5381810 2.038225 23.897835 90-94
TTAAA 6875655 2.002497 18.81023 50-54
TGCAG 2550380 1.991302 25.577696 4
TACTA 4736865 1.9223505 25.600254 65-69
TGACC 2604460 1.9151772 63.64526 1
TTCCG 2282255 1.9094759 18.4366 40-44
AGGGA 2616100 1.9062016 22.044245 9
TAAAC 5343130 1.9058055 21.435678 50-54
GGATG 2297160 1.9044278 19.639116 10-14
CCAAA 4348610 1.8995829 38.428986 4
GATTA 4376830 1.8860023 27.09269 50-54
ATTAA 6387975 1.8604629 18.825853 50-54
GATGT 2915445 1.8587121 18.117027 30-34
TCCGT 2167110 1.8131385 19.10726 40-44
CAGGA 2629615 1.8045365 15.228105 90-94
TAGGG 2173715 1.8020874 24.864702 8
TTGTT 3224980 1.798983 13.36168 35-39
CAGAG 2601695 1.7853769 15.415449 25-29
ATGAT 4142265 1.7849269 10.077657 15-19
AAGAG 3614885 1.7802634 11.428956 50-54
AGCTG 2273975 1.7754887 9.779015 95-97
TGATG 2776555 1.7701641 14.873912 30-34
AGGCT 2239590 1.7486416 17.18133 65-69
ATAGA 4605295 1.7441397 23.72541 90-94
GAGGA 2382440 1.735947 21.960882 95-97
ACTAA 4848445 1.7293596 22.694107 65-69
ACCAA 3919320 1.7120581 20.607061 3
GCTCA 2325795 1.7102623 20.750198 65-69
CTACA 3392080 1.6859031 12.34149 85-89
TGTTT 2999755 1.6733463 16.141521 35-39
TACAG 3162435 1.6688955 11.88868 90-94
GGGAT 2008940 1.665483 23.273668 10-14
AAGAA 4990340 1.6610976 10.377608 45-49
TCCTC 2079575 1.6386402 10.895896 1
CTCAC 2343540 1.6230155 15.15648 65-69
ATACC 3256585 1.6185607 11.0128 75-79
TACCA 3230525 1.6056083 11.538471 75-79
CATCA 3198300 1.5895922 10.090156 80-84
GAGCT 2034100 1.5881978 11.883949 95-97
GAGAT 2818840 1.5794975 15.982374 25-29
AACAG 3390055 1.5723727 15.441772 60-64
CCTCC 1626285 1.5693893 6.0426636 1
GACCA 2397780 1.5496794 27.438192 2
CAAAC 3491055 1.5249811 44.582676 5
GGAAG 2071285 1.5092263 10.377897 6
GGGGG 941470 1.5016562 6.5812173 1
GAAAC 3232750 1.4994116 10.417423 60-64
GAGAA 3044275 1.4992486 14.3394575 50-54
TCTAC 2615405 1.4789866 15.432491 85-89
AGCCA 2279235 1.4730641 16.238726 95-97
CCGTA 1982850 1.4580793 20.596695 40-44
TAAAG 3849085 1.4577441 9.518978 45-49
GTAAA 3777900 1.4307846 8.585671 45-49
TGCTG 1583450 1.4066802 7.276683 1
GATAC 2633835 1.3899401 10.175677 75-79
ATGTT 2818210 1.3817028 10.835638 30-34
GGCCA 1439080 1.3760754 24.035091 2
AGATG 2448315 1.3718791 12.357556 25-29
AGAAA 4100210 1.3648067 7.1819987 60-64
AAAGA 4047940 1.3474078 8.280254 45-49
AAGTA 3503775 1.3269666 23.364475 8
GCCAA 2029805 1.311858 18.940323 3
AGAAC 2805365 1.3011823 11.400489 50-54
CTCTT 1988545 1.2794386 7.721149 3
GCTCC 1247845 1.2786028 5.772677 1
TCCAA 2540240 1.2625288 5.575891 3
TCTTG 1840630 1.2574525 7.3805737 4
GAGCG 1230235 1.2490689 6.586381 90-94
CAACA 2831920 1.2370543 7.6463346 8
CGTAA 2327190 1.2281159 11.568394 40-44
CAATA 3430295 1.2235289 10.050352 80-84
GATAG 2174730 1.2185795 15.835186 15-19
GCTGG 1044665 1.2067965 6.456043 1
GCCAT 1636950 1.2037234 5.5222955 45-49
TCAAT 2951595 1.1978387 9.157792 80-84
AAACA 3813645 1.1955405 7.628 60-64
TGATA 2689175 1.1587816 10.13362 15-19
TGGCC 1062475 1.1559385 41.057503 1
ATCAA 3216960 1.147436 8.038434 80-84
GGATA 2045765 1.1463157 12.061536 70-74
CCGAA 1772835 1.145779 38.803925 4
CCTCT 1446615 1.1398875 9.056403 2
TATCT 2462705 1.1371367 9.989304 85-89
ATCTA 2747080 1.1148409 9.334693 85-89
CCCAA 1770515 1.0776832 8.68383 4
CCAAC 1768980 1.0767488 6.160852 5
GCCCA 1186770 1.0687665 8.200823 2
CGGAA 1546690 1.0613943 8.781554 5
TGCCA 1415080 1.0405723 5.61686 1
CTTTT 1939895 1.019148 5.4521937 6
TCAAC 2047130 1.0174475 8.799178 7
CCTTT 1580970 1.017203 5.062502 4
CCATA 2045790 1.0167813 5.7167172 3
TGTAG 1588495 1.0127287 5.593779 3
TTGAC 1686120 1.0124061 7.22325 6
AATAT 3324995 0.9683866 6.422206 80-84
CGAAC 1495735 0.96668994 37.13734 5
ACCTA 1926645 0.9575649 9.0664 3
GTTGC 1075440 0.95538235 6.9050894 1
CCTAA 1909160 0.94887465 12.323165 4
ATATC 2336260 0.94811875 7.8944707 85-89
GAACT 1791185 0.9452528 32.988503 6
GACCT 1252745 0.92120004 13.231262 2
GGTGC 772130 0.8919642 10.551521 2
CAACT 1791590 0.890441 5.2088356 6
TAAGT 2059500 0.88745093 5.1640105 5
ACCGA 1371065 0.886116 24.324654 3
CTCCG 864085 0.88538367 5.4401574 2
GTCCA 1201905 0.8838151 6.1332746 2
ATTGG 1383500 0.8820362 5.9910955 6
CTAAA 2453430 0.87509763 7.1733603 4
TGTCC 1044465 0.8738642 10.159198 1
GGCTG 747185 0.86314774 9.76955 1
GTGCA 1095790 0.85557795 7.9067836 3
GGGTG 681200 0.83555037 11.819025 1
TGCCC 811130 0.8311234 9.0045805 1
ACCCA 1359590 0.82755995 5.4404125 3
GCTGT 913825 0.8118094 9.044987 1
CTGTC 968135 0.8100018 11.294148 4
CCCCC 660860 0.78103137 5.391992 1
AACAA 2471645 0.7748366 5.858019 9
GCAGG 753005 0.7645328 5.622507 1
GGCCT 701155 0.762834 7.7748017 2
TGTCA 1258160 0.7554438 8.709836 5
GGGCC 531770 0.7523256 10.494117 1
GACCG 773820 0.73994124 35.402428 2
CGACC 821290 0.73962706 7.7725687 1
TGGCT 823945 0.7319632 8.218599 1
CCGTC 712805 0.7303748 6.2189374 4
AAGTC 1374990 0.7256164 6.2062225 6
GTCAA 1373315 0.7247324 8.816848 6
AGTCT 1165055 0.69954026 8.379227 7
GGCCG 490255 0.693592 22.63017 2
GCCAG 706435 0.6755064 5.4544854 2
TGCGG 565110 0.6528148 14.092163 3
CTAAC 1294895 0.6435779 11.423454 5
GCCGA 651110 0.62260365 15.987802 3
TCTAA 1510525 0.6130127 6.0024114 9
TCCGA 833485 0.61289924 5.329867 3
TGACT 1013505 0.6085443 14.794603 1
CAGGG 588590 0.59760076 5.2378626 2
GACTG 765050 0.59734064 10.285706 2
TAACT 1452195 0.5893408 9.768362 6
GACCC 645285 0.5811227 7.6887355 2
GCCCC 459630 0.57677764 6.189203 1
ACTGT 945545 0.56773865 5.4118457 3
GGCCC 422630 0.56312054 7.4309516 1
GTCTA 929860 0.5583209 8.388547 8
GACTT 919160 0.5518962 9.493439 6
CCCCG 430475 0.54019177 5.7011366 2
GCGGA 512865 0.5207165 11.4678955 4
GCCCG 364440 0.48558703 6.6481853 2
GGACC 485245 0.4640004 5.7291307 1
CCCGA 512210 0.4612797 5.671455 3
GTCCG 417655 0.45439515 6.5913467 2
CCCGT 436805 0.44757172 5.3188996 3
GACTA 711130 0.37528095 6.540025 2
Produced by FastQC (version 0.10.1)