FastQCFastQC Report
Thu 31 Jul 2014
20L22A_TGACCA_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20L22A_TGACCA_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 17203636
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 356532 2.0724223646675624 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 157717 0.9167655023624075 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 147711 0.8586033789601221 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 136245 0.791954677487945 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 133126 0.7738247891317859 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 123084 0.7154534076401059 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 121700 0.7074085966478248 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 90053 0.5234532978958634 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 85230 0.4954185266416936 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 78774 0.45789157594359703 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 76581 0.44514427066464324 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 70728 0.411122392963906 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 70386 0.40913444111465735 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 69891 0.4062571423854818 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 69431 0.40358328902099533 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 63379 0.36840467910388247 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 62734 0.36465547166889606 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 62414 0.3627953997631664 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 59953 0.3484902842631639 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 57336 0.3332783837091182 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 55886 0.32484993288628056 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 54477 0.3166598037763645 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 51036 0.29665821806506487 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 50677 0.29457144989582434 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 50468 0.29335659043239465 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 50223 0.29193247287957036 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 48676 0.2829401877603084 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 44327 0.2576606480165007 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 40511 0.23547929054067407 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 39385 0.22893416252238774 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 39156 0.2276030485648499 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 38654 0.22468506076273645 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 38223 0.22217977641470674 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 38204 0.22206933464530404 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 36471 0.21199588273083667 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 36097 0.2098219236910151 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 34793 0.20224213067516655 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 33796 0.19644684414387747 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 33574 0.1951564192592775 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 33333 0.19375555260527483 No Hit
CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA 32034 0.18620482321295334 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 31216 0.18145001440393183 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 31133 0.18096755825338318 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 30689 0.17838670848418323 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 30533 0.17747992343014 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 30448 0.17698584183018057 No Hit
ATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGAC 29235 0.16993500676252393 No Hit
TACCATGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTC 28110 0.16339569146894298 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 27416 0.15936166052339168 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 25966 0.15093320970055402 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 24845 0.14441714530579466 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 24139 0.1403133616637785 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 24058 0.13984253096264068 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 23750 0.13805221175337584 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 23549 0.13688385408758938 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 23160 0.13462270417718675 No Hit
ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC 22955 0.13343109561257865 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 22486 0.13070492772574355 No Hit
GCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCT 21460 0.12474107217799772 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 21309 0.12386335074748153 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 20310 0.11805643876678162 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 20228 0.11757979534093839 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 20172 0.11725428275743568 No Hit
GCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCAAT 20168 0.11723103185861407 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 20110 0.11689389382570058 No Hit
ATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCA 20076 0.1166962611857168 No Hit
TATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGA 19310 0.11224371406137633 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 19276 0.11204608142139254 No Hit
ACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGACTG 19222 0.11173219428730066 No Hit
ATGCACCCATTCCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCT 19066 0.11082540923325744 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 18709 0.10875026651342774 No Hit
CAGCTCCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGT 17831 0.10364669422208189 No Hit
ACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGAT 17785 0.10337930888563325 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 17662 0.10266434374686839 No Hit
TACTTGGTGTAGCTTGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGT 17215 0.10006605580355223 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 8032075 4.864097 7.115154 70-74
CTGCT 5985310 3.773204 13.547173 4
ACTTC 6160080 3.629913 7.386041 60-64
AGCTG 5026830 3.4368763 7.726667 3
AACTT 6687695 3.2474618 6.3279805 60-64
CTACT 5285795 3.1147285 13.349276 2
CAAGC 3149030 3.0043423 9.760252 75-79
CTTCT 6121980 2.9324276 5.9443116 40-44
TGCTA 5597425 2.9078348 8.671512 4
ACAAC 3201310 2.8548715 9.414808 85-89
CAACT 3936130 2.8533416 9.650279 9
GCTGC 3434400 2.8494616 11.015165 3
ACCCA 2627590 2.8435354 46.360825 1
TCAAG 4436895 2.8355355 5.9785523 10-14
CTGGT 5005600 2.781963 11.024398 1
CTTCA 4698110 2.768427 7.277158 60-64
GGAAG 3689605 2.735871 7.28132 15-19
CGGTG 3725570 2.7250607 11.919199 9
GCTAC 3484925 2.7026608 17.77283 1
AGGAA 3889170 2.6956213 7.287823 40-44
TTCAA 5502490 2.671941 7.6447816 55-59
TCTCT 5550275 2.658581 9.061385 20-24
AGCAC 2784375 2.656442 5.6967816 65-69
GGTGT 5400795 2.6462104 10.623961 9
ACTGC 3411220 2.6455004 12.637874 2
TGGTG 5291810 2.5928118 9.263125 8
ACATC 3554450 2.5766575 7.1317196 90-94
TATGC 4924375 2.5581884 8.856182 8
GGTGG 3918170 2.5266101 7.023455 7
AAGCT 3929295 2.511138 6.191496 75-79
CAGCA 2622610 2.5021095 6.8914638 60-64
GCAGC 2428665 2.4788713 7.945534 1
CAACA 2772750 2.4726896 8.720368 85-89
CTGAA 3865840 2.470585 6.5079255 80-84
TCAAC 3401310 2.4656453 7.629164 8
GAAGC 2855710 2.4019184 8.158916 15-19
CCAAG 2511090 2.3957136 8.497663 75-79
ATGCA 3705745 2.368271 6.040446 1
ATGGT 5095565 2.3337018 5.416255 1
TGAAA 4421810 2.3287013 5.742961 70-74
CTTTA 5892920 2.3260748 7.729317 5
CAGCT 2980725 2.311639 6.992092 2
TCTGC 3624170 2.284716 5.4491334 85-89
CATGG 3334945 2.2801232 14.69531 2
CGTGA 3302835 2.2581697 12.434383 9
CTTGG 4039315 2.2449307 9.115216 6
CCGTG 2691135 2.2327871 5.9433026 60-64
TCCTT 4646265 2.2255602 6.3472805 40-44
TAGCT 4282460 2.224717 5.6915774 2
CCAGC 1919910 2.2227762 6.8271046 1
CAAGG 2623515 2.206621 7.726725 10-14
GCTGG 2951470 2.1588466 8.526102 4
GCTTC 3388860 2.1363738 8.583772 15-19
CTCCT 2987270 2.1361246 7.6182923 70-74
GCTCC 2229605 2.098303 6.171213 70-74
GTTCC 3322370 2.094458 5.332775 70-74
CCTTA 3552265 2.0932214 8.542981 95-97
CTGCA 2689105 2.0854795 5.308198 95-97
CTCTG 3280185 2.0678642 8.371129 20-24
CTCTC 2821780 2.0177865 5.01335 15-19
CACAA 2251525 2.0078702 7.8021164 85-89
GAAAG 2886730 2.0008202 5.5236087 7
CCGCC 1411665 1.9833012 7.0707984 75-79
TTCCA 3344000 1.9704983 5.8914895 75-79
TTCGG 3543800 1.9695381 9.0707655 7
AAGGA 2834920 1.9649106 6.875528 10-14
TCGGT 3533165 1.9636276 11.15453 8
AGCTC 2522145 1.955997 5.118005 70-74
CCCAA 1804845 1.9531742 46.145992 2
ACCAG 2031645 1.9382975 5.5527644 60-64
CTATG 3691405 1.9176668 5.9409647 35-39
TATTC 4833960 1.9080783 5.9947267 5
GGTAT 4152985 1.9020121 7.4923744 6
GCCTT 2965160 1.8692689 5.326482 35-39
CCTGT 2954815 1.8627472 7.644246 1
CATCC 2117460 1.862695 7.7967916 90-94
TTTCA 4712175 1.8600069 6.372479 8
CCAAT 2559355 1.8553032 31.119919 3
ATCGG 2706480 1.8504378 5.6283545 85-89
CCACA 1700675 1.840443 9.957846 1
CACCC 1391340 1.8271596 6.844668 50-54
GTGTA 3983520 1.8243998 9.390637 2
TGTAG 3972275 1.8192495 5.0683312 3
TTGGT 4882850 1.8178241 18.447832 7
CTCTA 3076845 1.8130739 5.1067634 95-97
GTGGC 2449855 1.7919414 5.781985 8
TCGGA 2615600 1.7883027 5.877918 85-89
GCACC 1543985 1.7875487 6.1735587 50-54
TGCTT 4210050 1.7778461 5.8934126 4
GTAGC 2598565 1.7766558 6.507312 1
AAACC 1989825 1.7744908 5.135012 70-74
GCACT 2280950 1.7689432 5.228308 35-39
TCTGG 3167335 1.7603102 5.163173 45-49
GCAAG 2091940 1.7595167 6.0455775 25-29
ACGGT 2565465 1.7540249 11.423827 4
AGAAA 2700475 1.749558 9.77344 9
TACTT 4409340 1.7404708 9.184163 3
GCTTG 3123375 1.7358785 6.5946946 5
CATGC 2237890 1.735549 8.780154 2
CTTTC 3591095 1.720134 7.5468364 7
TGGTA 3742400 1.7139701 8.61556 2
ACACA 1895585 1.6904492 7.5111957 85-89
AGCCT 2156410 1.6723588 6.0262523 2
AATGA 3129845 1.648301 5.306023 75-79
GAACA 2095290 1.6473068 6.8509927 80-84
TCCAA 2261845 1.6396352 6.3471165 75-79
GGTCG 2234950 1.6347497 12.888564 6
TTTAG 4694120 1.6334991 6.408232 6
AACAC 1823435 1.6261071 7.5976753 85-89
ACCGC 1400605 1.6215507 5.668783 75-79
ATGGG 2688450 1.6204783 12.38926 3
AAGCC 1694260 1.6164142 7.511988 2
ACATG 2512020 1.6053841 16.404036 1
TTAGG 3499165 1.6025714 5.9623785 35-39
CTACC 1816705 1.5981259 5.3686576 25-29
CTGTA 3069955 1.5948265 6.8502584 8
GCTGA 2306435 1.5769246 6.125957 80-84
ATGCC 2010650 1.5593177 12.169878 9
CATGA 2439665 1.5591434 5.4260225 65-69
GCAAC 1630830 1.5558987 9.064203 1
ACTTT 3927660 1.5503403 9.455872 4
GACGG 1703920 1.5332294 14.987738 3
AAAGC 1947145 1.5308361 5.5101924 20-24
TGAAC 2372195 1.5160247 6.151786 80-84
AACAT 2533870 1.5136534 5.354132 90-94
GGTAC 2213510 1.513391 10.760663 3
CTGCG 1821520 1.5112833 5.577512 45-49
GGGTC 2051185 1.5003351 12.818935 5
GACTG 2174185 1.4865044 10.157213 1
GCGAC 1451590 1.4815979 5.242821 20-24
ATTCC 2468115 1.4543712 5.437006 80-84
GCGGC 1330700 1.4530493 9.273674 7
CGCAG 1421290 1.4506717 5.3281074 90-94
GGCTG 1926325 1.4090065 6.883907 9
CTGAC 1811990 1.4052511 12.7947235 1
TGGGT 2862130 1.402349 9.170165 4
ATGCT 2696495 1.4008158 6.0705733 3
GTATT 4022060 1.3996301 5.9152923 4
GGAAT 2474305 1.3940576 5.0784984 40-44
GTCAA 2155855 1.3777661 5.574658 10-14
GGTCA 2006105 1.371587 25.981632 9
AACCG 1431375 1.365608 5.967405 70-74
TGGTC 2454965 1.3643962 21.420866 8
ATCTC 2307090 1.359485 5.4631276 45-49
GAATC 2090980 1.3363055 5.4695554 40-44
GTATG 2824850 1.293744 7.624685 7
GCGCA 1266410 1.2925899 5.5384116 25-29
CAATT 2660265 1.2917917 21.200941 4
CACAT 1773150 1.2853749 6.723026 2
TGTAT 3660890 1.2739471 5.719446 3
TGGGG 1963715 1.2662909 5.342753 9
GACGC 1231060 1.2565091 5.5058 15-19
AAGAC 1587375 1.2479867 5.460571 9
CGCAA 1307590 1.2475106 5.160442 25-29
TGCCT 1969975 1.2418935 5.286429 30-34
TGACG 1811775 1.2387223 11.191596 2
GCCTG 1491500 1.2374715 5.1774774 6
GTACT 2376955 1.2348164 8.202394 4
AGCGC 1193450 1.2181215 5.1793976 25-29
ATTCG 2340965 1.2161198 7.4976635 6
ATCCT 2041905 1.2032211 5.086918 90-94
AACGC 1241250 1.1842186 5.6696696 95-97
TGCGG 1526170 1.116314 6.5307927 6
CGGTA 1623545 1.1100283 10.861104 5
ATTGG 2418830 1.1077923 18.197037 6
CTTAT 2795520 1.103458 5.475798 95-97
GCTCA 1414270 1.0968076 5.772137 1
GCCTA 1410460 1.0938529 5.889776 3
TGGCC 1279810 1.0618359 7.558003 1
TAGAA 2002935 1.0548253 7.7656884 8
TCGTG 1885010 1.0476322 9.937137 8
CGGCT 1257725 1.0435123 6.905984 8
ACGCG 988065 1.0084907 6.2561274 15-19
AATTG 2270520 0.9719962 16.496393 5
TTAGA 1981820 0.8484054 6.7566233 7
CACGG 797820 0.81431293 5.9803824 4
CTACA 1100535 0.7977894 7.5032725 3
GTCGT 1385975 0.7702835 9.692259 7
GGCCT 912495 0.7570811 5.0692396 5
Produced by FastQC (version 0.10.1)