FastQCFastQC Report
Thu 31 Jul 2014
20L15A_ACTTGA_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20L15A_ACTTGA_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 18041326
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 312407 1.731618839989921 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 310500 1.721048663496242 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 196108 1.0869932731108567 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 157845 0.8749079751676788 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 157266 0.8716986766937197 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 130091 0.7210722759513353 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 109394 0.6063523268744215 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 99938 0.5539393279629224 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 97127 0.5383584332991932 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 82020 0.45462290299504593 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 77482 0.42946954120778047 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 74105 0.41075140485793565 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 72683 0.4028695008338079 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 71018 0.39364068915998746 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 67771 0.3756431206885791 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 67466 0.37395255758917056 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 60823 0.33713153900106896 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 58133 0.322221326747269 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 51534 0.285644192671869 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 49348 0.27352756665446876 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 48239 0.2673805683684226 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 48232 0.2673417685595837 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 45663 0.253102238715713 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 43134 0.23908442206520739 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 42306 0.23449495896255076 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 41983 0.23270462492612792 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 40705 0.22562088839811445 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 38400 0.21284466563045312 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 38250 0.21201324115533415 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 36456 0.2020694044329114 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 34963 0.19379395949056072 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 32628 0.18085145182787563 No Hit
AGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 31989 0.17730958356386886 No Hit
GGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 31590 0.17509799446005242 No Hit
TGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 31389 0.17398388566339304 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 30369 0.16833019923258413 No Hit
TGGCTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 29792 0.16513198641829321 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 29547 0.16377399310893223 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 29524 0.16364650802274733 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 28171 0.15614705925717434 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 27762 0.15388004185501664 No Hit
TGACGAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 27386 0.15179593783738513 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 26585 0.14735613114024987 No Hit
TTGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 25424 0.14092090570282917 No Hit
CTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCA 24217 0.1342307100930386 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 23530 0.1304227859969938 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 22921 0.12704720262801084 No Hit
GGCCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 21725 0.12041797814639568 No Hit
TGGTCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 20909 0.11589502900174854 No Hit
GGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 18989 0.10525279572022588 No Hit
TAGGTAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 18839 0.10442137124510693 No Hit
TAGATGAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 18361 0.10177189858439452 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 21060750 8.442794 14.094144 7
AGCAG 8094045 5.3849506 63.81477 20-24
TAGCA 9985970 5.09879 41.845318 20-24
CACGG 5598850 4.964483 56.765305 45-49
ACTTG 8695985 4.9234962 126.556496 8
CCTGC 5018620 4.551759 59.07849 15-19
GCAGA 6749265 4.490271 43.627407 25-29
CTGCG 4487580 4.4123025 63.161583 15-19
TCACG 5918680 4.027734 43.647976 45-49
GTTTC 6339230 3.979873 43.96216 40-44
GAAGT 7166010 3.966545 35.612408 60-64
CGGAT 5329905 3.932 44.20713 45-49
GAACC 6097805 3.7422388 39.06271 70-74
AGAGA 7346780 3.6673646 30.100271 90-94
CTTGA 6407485 3.6277924 36.723686 55-59
ACGGA 5424225 3.608725 41.96869 45-49
GCGTT 4408150 3.6060212 49.35319 15-19
AACTT 9137495 3.5806732 87.728264 7
AGTAG 6361095 3.5210068 33.619923 35-39
TGCGT 4271975 3.4946258 52.015274 15-19
GAGCC 3809130 3.3775434 83.910255 95-97
CCATG 4868390 3.3129983 43.877422 75-79
ACCTG 4852480 3.3021717 34.344612 15-19
CTTGT 5259195 3.3018093 140.44617 9
CATGC 4744905 3.2289658 43.497383 75-79
TTCTC 5568615 3.2249413 35.642696 30-34
GAGAG 4423725 3.1905262 57.622547 95-97
AGAGC 4773145 3.1755629 68.23083 95-97
ATAGC 6055220 3.0917673 32.52487 80-84
CACTG 4541665 3.0906584 43.025715 85-89
CTCAG 4531055 3.0834382 43.601166 35-39
AACCA 7190785 3.0543375 25.850334 70-74
TGAAG 5509505 3.049633 27.645674 60-64
GCAGC 3398420 3.0133684 19.451199 20-24
TGCAT 5321950 3.0131836 34.358303 75-79
TTCAC 5762615 3.0096517 27.257238 45-49
AGCAC 4881970 2.9960778 36.741875 80-84
GCACT 4402035 2.9956384 33.140373 85-89
GGAAC 4472595 2.975608 42.59487 70-74
ACCAT 6302455 2.968441 33.95256 75-79
GACTC 4351130 2.9609969 43.14057 25-29
AAGTT 6924625 2.9416568 28.22358 60-64
TTGAA 6741305 2.8637803 26.020834 55-59
GGATT 4560180 2.7989485 30.401651 50-54
CAGAC 4491235 2.7562826 39.104965 25-29
AAACT 7758910 2.7419553 46.971436 6
GTAGT 4448125 2.7301712 30.399908 40-44
TTTCA 6271895 2.7252998 26.852585 40-44
AAGGA 5342370 2.6668036 24.88139 70-74
ATGCA 5211405 2.6609192 32.836693 75-79
AGGAG 3662125 2.641237 20.392832 95-97
AGAAG 5286220 2.6387746 18.415953 6
TTAGC 4659245 2.637973 36.5428 20-24
TTGTA 5560505 2.619316 35.69957 9
GTTAC 4603345 2.6063237 33.963833 60-64
GTTAG 4245900 2.6060498 39.48496 20-24
CTGAA 5078665 2.5931427 32.186 85-89
TCAGT 4577630 2.5917642 36.484898 35-39
CAGTA 5061650 2.5844545 32.48602 35-39
AGGAA 5152825 2.5721862 32.125122 70-74
CATTC 4881085 2.5492535 33.71455 30-34
TCTCA 4855620 2.5359538 26.058918 35-39
TAGAG 4549965 2.5185063 34.50513 90-94
AGTTT 5326705 2.509183 30.611763 40-44
CATAG 4876415 2.4898748 32.568863 80-84
GCATA 4868065 2.485611 25.228876 80-84
CTCAT 4718620 2.4644024 25.893993 30-34
TAACC 5229995 2.4633152 29.910131 10-14
CGTTA 4304830 2.43731 27.799175 20-24
TAAGG 4384825 2.4270976 33.26134 65-69
CTAAG 4690445 2.3949194 32.346733 65-69
GTAAC 4681835 2.3905232 32.827976 10-14
GCTGC 2421835 2.3812096 29.933207 1
AACCT 5050610 2.378825 28.670818 10-14
TGTAA 5564060 2.3636737 27.74254 10-14
AGACT 4545590 2.3209572 32.790215 25-29
ACTGA 4477375 2.2861269 32.578495 85-89
ACAGG 3434755 2.285135 13.823689 90-94
TGAAT 5280140 2.2430615 26.09645 85-89
ATTCT 5053595 2.1959171 28.017012 30-34
TCATT 5053395 2.1958303 27.968761 30-34
TAGTT 4654860 2.1927056 30.18825 40-44
ACTCA 4648820 2.189583 28.47059 25-29
GGAGG 2097510 2.185725 17.599539 90-94
AGTTA 5117530 2.1739829 26.916191 60-64
GAATA 5581645 2.138356 19.097208 90-94
GTTGT 3108665 2.1157522 12.996215 35-39
AATAG 5410870 2.072931 24.65776 90-94
GATGA 3735530 2.0676985 9.868856 15-19
CTGCA 2999375 2.0411112 16.979643 3
ACCAG 3318645 2.0366611 12.547704 55-59
TTACT 4668270 2.0284836 21.442518 65-69
CAGCA 3276880 2.0110297 14.932567 20-24
CAGAA 4362485 2.0087821 13.285542 5
TTAAA 6812780 2.0031013 19.231258 50-54
GGAGC 2050295 1.9708325 19.57263 95-97
GAAGA 3943880 1.9687055 8.868042 50-54
TGTTG 2839330 1.9324431 11.689808 35-39
GGCTC 1960540 1.9276527 18.721823 65-69
CAGGC 2160915 1.9160765 14.563758 65-69
TAAAC 5386530 1.9035695 21.618525 50-54
CCAAA 4438050 1.8850938 39.14572 4
GATTA 4422085 1.8785503 27.128166 50-54
TACTA 4769825 1.8691323 25.091976 65-69
ATTAA 6345310 1.8656553 19.232883 50-54
CCAGA 3008510 1.8463304 15.589057 55-59
TGCAG 2498720 1.8433664 19.978968 4
TTTCC 3158300 1.8290601 10.538679 40-44
TGACC 2658920 1.8094275 61.537453 1
CCATC 2870065 1.8016459 11.806118 75-79
GTAGG 2235465 1.7878046 18.751802 7
ATAGA 4615655 1.7682804 24.491816 90-94
AGGGA 2424030 1.748285 16.973877 9
ACTAA 4946340 1.748009 22.81466 65-69
GGGGG 1156960 1.7419151 7.381839 1
GGATG 2150340 1.7197264 16.020618 10-14
TCCTC 2444120 1.7012894 10.627526 1
AAGAG 3379005 1.6867312 9.737159 50-54
ACCAA 3929770 1.6691982 20.506292 3
TGATG 2691875 1.6522198 12.078185 30-34
GATGT 2680015 1.6449403 14.357225 30-34
CAGAG 2461885 1.6378868 12.556814 25-29
TTCCG 2166705 1.6349845 13.923189 40-44
AGCTG 2197905 1.6214477 7.4535594 95-97
CCTCC 1933265 1.61744 5.725872 1
AAGAA 4676555 1.6157193 8.977172 45-49
CAGGA 2417795 1.6085538 12.268985 90-94
TTGTT 3073040 1.6051655 10.551036 35-39
GAGGA 2216740 1.5987811 18.058191 95-97
CAAAC 3697450 1.5705186 45.51047 5
ATGAT 3687840 1.5666349 8.426016 15-19
GGAAG 2158240 1.5565892 8.416172 6
GAGCT 2102350 1.5509546 9.145052 95-97
TAGGG 1901405 1.5206413 18.672668 8
AACAG 3278775 1.509769 13.34648 60-64
GACCA 2452195 1.5049185 26.854286 2
GCTCA 2201650 1.4982497 15.886885 65-69
TACAG 2926410 1.4942114 9.892784 90-94
AGCCA 2432560 1.4928684 15.916963 95-97
TCCGT 1977215 1.4919963 14.365965 40-44
TGTTT 2843620 1.4853306 12.472905 35-39
AGGCT 2007875 1.4812582 13.55422 65-69
CTACA 3139510 1.4787017 9.904831 85-89
CATCA 3110470 1.465024 8.081063 80-84
GAGAT 2638840 1.4606562 13.021697 25-29
GGGAT 1818990 1.4547303 18.456295 10-14
GAGAA 2889930 1.4425948 12.016374 50-54
ATACC 3035620 1.4297698 8.785743 75-79
GAAAC 3102540 1.4286187 8.711319 60-64
TACCA 2986825 1.4067875 9.188181 75-79
CTCAC 2229075 1.399273 11.403062 65-69
TGCTG 1700440 1.3910197 6.214631 1
GGCCA 1531660 1.358118 24.231129 2
AGAAA 3910895 1.3511888 6.3275175 60-64
CAACA 3108005 1.3201475 7.6158023 8
GCCAA 2146575 1.3173586 19.207298 3
TAAAG 3413300 1.3076522 7.935093 45-49
AAAGA 3781230 1.3063903 7.219598 45-49
TCTTG 2056195 1.2909132 7.269929 4
GTAAA 3363140 1.2884357 7.179281 45-49
TCCAA 2721935 1.2820249 5.616513 3
CTCTT 2201460 1.2749271 7.4159164 3
ATGTT 2706000 1.274681 8.621577 30-34
GCTCC 1401045 1.2707117 5.313843 1
AAGTA 3309470 1.2678745 19.936136 8
AGATG 2284405 1.2644687 10.091258 25-29
TCTAC 2410800 1.259093 11.632775 85-89
GAGCG 1297115 1.2468431 6.743334 90-94
GATAC 2430615 1.2410607 8.210312 75-79
GCCAT 1783985 1.2140235 5.1203833 45-49
GCTGG 1130130 1.204591 6.1843777 1
CCGTA 1761700 1.1988583 15.616141 40-44
TGGCC 1216645 1.1962363 40.692444 1
AGAAC 2565410 1.1812878 9.362602 50-54
CCTCT 1675505 1.1662762 8.660753 2
CAATA 3202645 1.1317966 8.192001 80-84
AAACA 3544200 1.1295356 6.541888 60-64
CCGAA 1836770 1.1272305 38.182068 4
TCAAT 2849315 1.1165497 7.418431 80-84
ATCAA 3109225 1.0987828 6.6974263 80-84
CCAAC 1938740 1.0975375 5.2869554 5
GCCCA 1333675 1.0908549 7.7264533 2
TCAAC 2295485 1.0811679 8.643183 7
GATAG 1943610 1.0758312 12.847209 15-19
CCCAA 1893420 1.0718814 8.387681 3
CGTAA 2093840 1.069105 9.193801 40-44
TGATA 2494940 1.0598779 8.401994 15-19
TGCCA 1543780 1.0505611 5.3534656 1
TTGAC 1813060 1.0265191 7.074542 6
CTTTT 2126090 1.0244131 5.1700974 6
GGATA 1839945 1.0184503 9.777958 70-74
CGGAA 1528835 1.0171305 6.991489 5
TATCT 2273130 0.98773354 7.8217154 85-89
GAACT 1928660 0.9847649 32.663517 6
CCATA 2067420 0.9737498 5.25294 3
ATCTA 2476300 0.9703778 7.446008 85-89
CGAAC 1580745 0.9701073 36.5261 5
TAAGT 2228200 0.94656384 6.1915545 5
GGTGC 885030 0.9433421 10.355738 2
GTTGC 1147295 0.93852764 5.6628723 1
CTCCG 1029385 0.93362576 5.4441757 2
GTCCA 1364310 0.9284296 6.12393 2
AATAT 3095275 0.91007614 5.3414154 80-84
GGGTG 787400 0.9098395 12.148726 1
GACCT 1324810 0.90154934 12.199656 2
GTGCA 1221290 0.9009753 7.8386207 3
CCTAA 1911995 0.90054506 11.3453865 4
ACCTA 1910985 0.9000694 8.516633 3
GGCTG 842920 0.8984576 8.408416 1
TGTCC 1178030 0.8889353 10.167293 1
ATTGG 1433295 0.8797282 5.14741 6
CTAAA 2483075 0.8775049 7.432247 4
ACCGA 1408920 0.8646579 23.861809 3
TGCCC 934105 0.84720916 8.616839 1
TCAAA 2387305 0.8436603 5.191377 4
ATATC 2144740 0.8404507 6.329301 85-89
CTGTC 1109380 0.8371324 10.649371 4
AACAA 2607375 0.83096963 6.096837 9
GGGGT 716615 0.8280476 5.257701 1
GCTGT 1006025 0.82296383 7.3062406 1
AAGTC 1597955 0.81590843 7.509825 6
TGTCA 1426180 0.807475 8.619514 5
GGCCT 809550 0.79597014 6.570205 2
GTCAA 1543675 0.7881933 8.926199 6
TGGCT 946010 0.77386945 8.356567 1
CCGTC 845950 0.7672548 6.1607757 4
AGTCT 1352855 0.76596 9.721426 7
GGGCC 595640 0.7630908 9.867655 1
GACCG 851740 0.75523514 34.12319 2
GGCCG 582575 0.74635285 21.969992 2
GCCAG 829995 0.73595387 5.231256 2
TGCGG 623550 0.6646339 10.73232 3
GCCGA 745520 0.6610502 15.586799 3
CTAAC 1361485 0.6412562 10.4451475 5
GACTG 859445 0.6340334 9.550854 2
TCTAA 1607635 0.6299775 7.0405455 9
TGACT 1111135 0.6291028 14.448151 1
TCCGA 906190 0.6166734 5.2866797 3
CCCCG 558775 0.60913426 5.7433224 2
GTCTA 1072360 0.60714906 9.717305 8
GGCCC 507825 0.60013413 7.0482936 1
GCCCC 550305 0.59990084 6.1620784 1
ACTGT 1056905 0.59839886 5.175045 3
TAACT 1525890 0.59794444 9.101793 6
GACTT 1030855 0.58364975 9.231973 6
GACCC 682710 0.5584101 6.3974404 2
GCGGA 564835 0.5429438 8.963768 4
CCCGA 640720 0.5240652 5.466113 3
GCCCG 433385 0.5121628 5.8058977 2
GTCCG 509560 0.5010123 6.578792 2
GGACC 544370 0.48269117 5.87271 1
CCCGT 518225 0.47001672 5.089553 3
GACTA 750305 0.3831022 6.651729 2
Produced by FastQC (version 0.10.1)