FastQCFastQC Report
Thu 31 Jul 2014
20L15A_ACTTGA_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20L15A_ACTTGA_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 18041326
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 449883 2.4936249142662796 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 274089 1.5192286864058662 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 177253 0.9824832165884038 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 149691 0.8297117407002125 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 127099 0.7044881290876291 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 104711 0.5803952547612077 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 93664 0.5191636135836135 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 88582 0.49099495236658325 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 84626 0.4690675175427793 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 82274 0.456030781772914 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 77867 0.43160353069391905 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 75187 0.41674874673846035 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 73605 0.4079799899408724 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 72674 0.40281961536530075 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 67913 0.376430202525025 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 67398 0.37357564516044994 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 63682 0.3529784894968363 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 62030 0.3438217346108595 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 61284 0.33968678355460125 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 59632 0.33053002866862446 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 52542 0.2912313651446684 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 52002 0.2882382370342401 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 51045 0.28293374888298123 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 50850 0.28185289706532657 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 47264 0.26197630928014937 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 46792 0.25936009359844175 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 46356 0.25694341979076263 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 44502 0.2466670132782923 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 43440 0.24078052799445004 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 42461 0.23535409758684034 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 42368 0.2348386144122666 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 40496 0.22446243696278198 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 39746 0.22030531458718722 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 39681 0.219945030647969 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 39117 0.21681887462152175 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 36809 0.20402602336435804 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 34731 0.1925080229690434 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 33638 0.18644970996034327 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 33314 0.18465383309408634 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 32472 0.1799867703737519 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 31567 0.17497050937386752 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 30865 0.17107944283031082 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 30665 0.16997087686348553 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 29924 0.16586363995639788 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 28123 0.15588100342513628 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 27813 0.15416272617655707 No Hit
CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA 27363 0.15166845275120022 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 27336 0.1515187963456788 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 27317 0.1514134825788304 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 26136 0.14486740054472713 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 25586 0.14181884413595763 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 25169 0.13950748409512695 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 24933 0.1381993762542731 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 24822 0.1375841221426851 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 23311 0.1292089062633201 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 23264 0.12894839326111618 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 23244 0.12883753666443365 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 22318 0.12370487623803261 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 21934 0.12157642958172808 No Hit
CAGCTCCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGT 21451 0.11889924277184503 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 20445 0.1133231559587139 No Hit
GCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCT 20113 0.11148293645378395 No Hit
ATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGGAT 19710 0.109249176030631 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 19344 0.10722050031134074 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 19309 0.10702650126714632 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 18832 0.10438257143626806 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 18778 0.10408325862522523 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 18349 0.101705384626385 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 18193 0.10084070317226128 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 18133 0.10050813338221369 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 7062285 4.244464 6.135203 90-94
CTGCT 5962060 3.5773587 10.802968 4
ACTTC 6135820 3.566518 8.088333 60-64
AACTT 6898510 3.352832 7.169301 60-64
AGCTG 4893155 3.081785 7.592636 3
CAACT 4256465 3.008771 9.745315 55-59
CTACT 5139050 2.9871337 10.827729 2
TTCAA 5877480 2.8565884 8.793982 55-59
CTTCA 4870010 2.8307512 8.160238 60-64
TCAAG 4561960 2.7833674 6.8578043 10-14
AGGAA 4325065 2.7698667 8.113446 40-44
TCAAC 3911775 2.7651196 9.429198 55-59
ACCCA 2684165 2.7595155 54.278324 1
GCTGC 3641070 2.7425702 11.199151 3
CAAGC 3062635 2.717677 10.25426 75-79
CTTCT 5668160 2.709229 6.635965 20-24
TGCTA 5366420 2.6923742 6.3734994 1
ACAAC 3119555 2.6816428 10.112782 85-89
GGAAG 4035540 2.6678553 7.9894476 15-19
TCTCT 5503560 2.6305547 9.869096 20-24
ACTGC 3597700 2.6251817 7.159391 4
GCTAC 3570765 2.6055274 14.278847 1
CTGGT 4979020 2.57863 10.3810835 1
AAGCT 4215895 2.5722244 7.1366377 15-19
GAAGC 3240655 2.482072 9.049518 15-19
TATGC 4942635 2.479758 8.787589 30-34
CAGCA 2703665 2.3991394 7.2561555 2
CAACA 2783065 2.392388 9.659801 85-89
AGCAC 2695705 2.392076 5.875163 35-39
ACATC 3376230 2.3865585 7.8490252 90-94
CGGTG 3663925 2.3820686 14.039029 9
GGTGT 5269515 2.3555632 11.993056 1
CCAAG 2648310 2.3500192 9.684354 75-79
GCAGC 2557065 2.3422794 9.145381 1
CTGAA 3796405 2.316283 7.2628098 80-84
CAAGG 3020310 2.313306 8.563605 10-14
TGGTG 5161570 2.30731 7.282598 8
CAGCT 3142045 2.2926977 7.7888656 65-69
GGTGG 4044985 2.2698836 7.2884326 7
TCCTT 4739515 2.2653615 8.136609 40-44
CATGG 3532455 2.224795 21.272488 2
TGAAA 4298340 2.1928275 5.4017763 65-69
CTGCA 2969385 2.1667106 5.925521 95-97
GCTCC 2449725 2.1377997 7.935056 65-69
CTCTG 3560825 2.1365685 9.252059 20-24
CTCCT 3044040 2.1161067 9.76427 70-74
CTTGG 4041320 2.092996 6.873145 2
ATGGT 4827370 2.090453 5.7558384 1
CGTGA 3296795 2.0763726 18.948093 9
AAGGA 3231695 2.0696487 7.8486304 10-14
CCCAA 1998150 2.0542426 54.66075 2
CACAA 2375955 2.0424268 9.188481 85-89
CTTTA 5107230 2.0411441 5.5113597 5
GCTTC 3399585 2.039821 9.930418 15-19
TCTGC 3398230 2.039008 5.3675303 85-89
CCAGC 1895045 2.0111177 8.007743 1
AGCTC 2746880 2.0043523 6.9760747 65-69
GTTCC 3332045 1.9992956 6.184357 70-74
TTTCA 5001190 1.9987644 5.909143 8
CCTTA 3434815 1.996527 10.123077 95-97
TAGCT 3958605 1.9860628 5.5616083 2
GGTAT 4533835 1.9633399 7.2079396 6
TTCCA 3364075 1.9554085 6.862171 75-79
GCTGG 2970935 1.9315274 8.426382 4
CTCTC 2756010 1.9158787 5.372434 15-19
CCAAT 2689665 1.9012457 37.624256 3
CCGTG 2521375 1.8991802 5.677515 60-64
CATCC 2213500 1.8712635 8.932345 90-94
TATTC 4657880 1.8615578 7.717841 5
TGGCT 3587230 1.857823 5.133943 9
TGCTG 3579405 1.8537704 5.1994905 6
CTTTC 3802175 1.8173379 6.965136 7
CTCTA 3105365 1.8050302 5.1192846 25-29
TACTG 3581230 1.7967306 5.0763183 85-89
CCTGT 2991875 1.7951868 7.439467 1
GAAAA 2885270 1.790022 5.061849 40-44
GCCTT 2970075 1.782106 6.254086 35-39
CACCC 1436315 1.7659917 6.3862805 50-54
TTCGG 3388045 1.7546654 11.10716 7
ACGGT 2772810 1.7463588 10.693429 4
ACACA 2026515 1.7420399 8.90764 85-89
TCGGT 3361390 1.7408608 13.501808 8
CTACC 2049940 1.732992 7.371902 25-29
GCTGT 3336275 1.7278538 5.3395705 7
GAACA 2326530 1.726219 8.191681 80-84
TCTGG 3325580 1.7223148 5.766548 45-49
CTATG 3431515 1.7216176 5.10503 6
ACCAG 1938235 1.719923 5.522264 30-34
GCACC 1608285 1.7067935 5.902692 75-79
CCACA 1643160 1.6892872 7.5889378 1
AATGA 3305895 1.6865249 6.233476 75-79
TTGGT 4724115 1.6822169 19.903168 7
GCAGG 2125510 1.6805022 5.1753664 90-94
TTAGG 3878780 1.6796737 6.8477073 35-39
GTTGC 3240230 1.6781121 5.7002563 75-79
CCGCC 1318115 1.672964 7.6349254 45-49
TTCAG 3316675 1.6640015 5.52942 65-69
AGCTT 3308245 1.659772 5.7736816 15-19
TCCAA 2345645 1.6580681 7.565623 75-79
AACAC 1914025 1.6453408 8.946569 85-89
TGTAG 3799140 1.6451863 6.0372653 9
TACTT 4099565 1.6384228 7.54907 3
GCACT 2231525 1.6283064 5.6116323 35-39
GTGGC 2475075 1.609148 6.0059075 8
ACATG 2625230 1.6017194 21.500803 1
GTGTA 3693885 1.5996065 11.395726 2
TGGTA 3691300 1.598487 7.7914743 2
GCTGA 2523795 1.5895252 6.8954754 80-84
TTTAG 4604765 1.5884542 5.373301 35-39
CCTTT 3320365 1.5870458 5.2111073 35-39
ATGCC 2173060 1.5856453 11.632943 9
GCTTG 3046670 1.5778676 6.876451 1
GCGGC 1662500 1.5720013 8.855072 7
GCAAC 1763935 1.5652553 6.8617287 1
AGAAA 2493460 1.5469431 6.3124285 9
CATGC 2113785 1.5423937 6.236655 2
CTGTA 3068020 1.5392493 7.3883343 8
GACGG 1946245 1.538769 13.653794 3
GCAAG 1991430 1.5252697 5.8906703 1
CATGA 2489990 1.5192059 6.411639 65-69
TGAAC 2481825 1.5142243 6.928966 80-84
GGTAC 2403085 1.5135002 9.416689 3
GTAGC 2385240 1.5022612 6.4114313 1
AACAT 2528050 1.4942056 6.275641 90-94
GGGAA 2254435 1.4903843 5.5969453 15-19
GATCG 2365145 1.489605 6.106895 50-54
ATTCC 2556420 1.4859492 6.024985 80-84
TTCAT 3711955 1.4835116 5.925333 60-64
GGCTG 2276985 1.4803618 6.81893 9
TCAGC 2018015 1.4725119 5.7209663 65-69
ATGGG 2704875 1.4704137 18.274683 3
AATCT 2999000 1.4575818 5.246546 40-44
ACTTT 3640020 1.4547622 7.8520894 4
GGTCG 2228750 1.4490023 19.35612 6
GTCAA 2374825 1.4489408 6.474399 10-14
GAATC 2374330 1.4486389 6.441508 40-44
GGAAC 1883215 1.442386 6.257448 15-19
ACTAC 2039520 1.4416772 5.0551515 70-74
GTGTT 4046195 1.4408153 5.2553115 70-74
GGAAT 2730445 1.4379091 5.8129725 40-44
CGCAG 1566535 1.434951 5.8827505 90-94
AACCA 1669100 1.4347975 5.1816134 75-79
TCATG 2810120 1.409859 5.391788 60-64
AGGTA 2656990 1.3992262 6.1690273 35-39
ATCTC 2400850 1.3955222 6.541152 45-49
TGGGA 2554970 1.3889227 5.8140197 15-19
CAATT 2820020 1.3705935 26.027967 4
ACCGC 1285130 1.3638451 5.7101717 45-49
GTATG 3140460 1.3599503 7.457097 7
AAGCC 1525725 1.353876 8.291151 2
GGTCA 2147030 1.3522328 29.92124 9
TGGTC 2601855 1.3474983 24.86327 8
CTGAC 1834970 1.3389468 12.206316 1
AGCTA 2185970 1.3337157 5.3622103 20-24
GTATT 3827500 1.3203298 7.1160126 4
GGGTC 2030010 1.3197932 19.276146 5
AGCCT 1807965 1.3192418 6.6133795 2
TGCCT 2179215 1.3075737 6.173642 30-34
TTCTC 2720160 1.3001636 5.886023 20-24
TGGGT 2901790 1.2971495 13.839362 4
GTACT 2565295 1.2870283 7.471898 4
TGACG 2040135 1.2849087 10.639754 2
ATCCT 2135665 1.2413803 6.0881686 90-94
GTGAG 2276930 1.2377757 5.7294216 10-14
CAGGT 1964225 1.2370994 5.3775206 95-97
GCGAC 1329100 1.2174597 5.7293844 20-24
TAGGA 2281810 1.2016486 5.450783 40-44
TGTAT 3480215 1.2005308 7.0231757 3
CGCTG 1586565 1.1950514 6.1389165 50-54
TGCGG 1826650 1.1875805 6.3985705 6
TCTGA 2364120 1.1860973 5.302836 20-24
AACGC 1323595 1.1745127 5.1237545 90-94
GCCTG 1546895 1.1651707 7.990043 6
TCGTG 2220630 1.1500622 15.444086 8
ATTCG 2267535 1.1376398 9.524751 6
ATTGG 2608035 1.129388 21.50375 6
CGGTA 1789455 1.1270266 10.22421 5
CGGCT 1492265 1.1240216 6.8549194 8
CCTTG 1869070 1.1214806 5.8151093 45-49
GTGGG 1996120 1.1201425 5.350392 10-14
TGGCC 1476680 1.1122824 10.674805 1
TACAT 2275425 1.1059079 5.6792097 4
CTTAT 2709910 1.0830365 6.7933006 95-97
ACCGT 1479410 1.0795007 5.5594745 25-29
TGGGG 1903455 1.0681427 6.0467186 8
GCTCA 1444525 1.0540457 6.2596855 1
AATTG 2484515 1.0422624 20.224035 5
GGATC 1653025 1.0411009 5.2900834 45-49
CGTCC 1176595 1.0267783 6.4951963 40-44
TCTTG 2466965 1.0177604 5.3560224 95-97
TTGGC 1895810 0.9818382 5.4983797 3
TTATG 2827880 0.9755022 6.015891 95-97
GGGGT 1724955 0.9679757 5.4722114 9
ATGCG 1512755 0.9527566 5.004708 35-39
TACCG 1287015 0.9391132 5.4627566 25-29
GCGTC 1234290 0.92970663 5.5733414 40-44
ATCGC 1266600 0.9242167 5.4609976 50-54
TAGAA 1692990 0.8636904 5.0176554 8
GCCTA 1164640 0.8498183 6.491944 3
CTACA 1191325 0.8421129 8.5013485 3
GTCGT 1623930 0.8410318 15.2543 7
GGCCT 1097675 0.82680386 7.659524 5
GCATA 1292940 0.78885543 5.5467877 60-64
GGCCC 665395 0.72894007 6.448305 1
CCTAT 1237260 0.7191721 5.060186 4
Produced by FastQC (version 0.10.1)