FastQCFastQC Report
Wed 6 Aug 2014
1S15C_GATCAG_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1S15C_GATCAG_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14493416
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 244607 1.6877111648489218 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 164245 1.13323870645816 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 96478 0.6656677763199511 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 92408 0.6375860597667244 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 90282 0.6229173301863412 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 74444 0.5136401245917456 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 69804 0.4816255877841359 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 66742 0.4604987533649762 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 64877 0.44763084148002097 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 62348 0.43018153898294237 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 57290 0.39528293398878495 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 54783 0.3779854245541562 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 51584 0.3559133333370132 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 46577 0.32136661226035323 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 46521 0.32098022991957176 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 45676 0.31514999638456526 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 44858 0.30950605433529266 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 43298 0.29874254627066527 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 41301 0.2849638760110108 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 35075 0.2420064393376965 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 32720 0.22575768197090318 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 32280 0.22272182072190574 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 31677 0.2185613108738478 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 30746 0.21213770445835545 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 29609 0.2042927630035597 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 29307 0.20220905823720234 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 29072 0.20058763234285143 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 27548 0.19007251292586924 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 26073 0.17989547805707087 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 25319 0.1746931158258343 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 25031 0.1727060066446723 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 24930 0.17200913849433425 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 24766 0.17087759021061702 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 24006 0.16563382987143954 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 23069 0.15916882534800628 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 22786 0.15721621459012836 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 22764 0.1570644215276785 No Hit
CCTACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGC 22646 0.15625025873817464 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 22455 0.15493241896872345 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 21943 0.1513997804244355 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 21679 0.14957826367503702 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 21619 0.14916428259562825 No Hit
AACAAGTTTATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATT 20974 0.14471398599198423 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 20234 0.13960821934594303 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 20040 0.13826968052252142 No Hit
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 20022 0.13814548619869876 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 19927 0.13749001615630158 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 19880 0.13716573097743143 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 19669 0.13570989751484397 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 19416 0.13396427729667043 No Hit
ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC 18382 0.12683000336152636 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 18112 0.12496708850418702 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 17949 0.12384243990512657 No Hit
ACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGAT 17694 0.12208302031763942 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 17658 0.12183463166999416 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 17596 0.12140685122127177 No Hit
GCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAATG 17502 0.12075828086353141 No Hit
CATCTTTTCAAGAGTTATTAATTGAGTTTTAGGGAGGGATTTATGTCACC 17103 0.11800530668546325 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 16855 0.11629418489057376 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 16790 0.11584570538788096 No Hit
ATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGAC 16192 0.1117196939631071 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 15590 0.10756608379970602 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 15507 0.1069934099731906 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15480 0.10680711848745665 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 15456 0.10664152605569316 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 15400 0.10625514371491165 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 15332 0.10578596515824841 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 14968 0.10327447994316868 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 14578 0.10058360292701182 No Hit
GTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCC 14567 0.10050770639578688 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 6148540 3.8281384 9.87227 70-74
CTGCT 4831765 3.7307496 11.567764 3
AGCAC 3184450 3.444037 7.2445703 80-84
GGAAG 3832630 3.420982 6.65333 15-19
ACTTC 4910530 3.36258 7.1304674 60-64
AGCTG 3928105 3.261033 8.481454 1
AACTT 5570760 3.0734265 6.786547 7
GAAGA 3854985 3.0516202 6.044281 90-94
CTGGT 4287670 3.0076127 16.295534 1
CGGAA 3021365 2.968567 7.861457 95-97
CTTCT 4967635 2.8742352 5.7601027 20-24
TGCTA 4575860 2.8466048 8.798539 4
CTACT 4144040 2.8377109 12.2164 2
CTGAA 3818635 2.8114743 6.9276376 80-84
TCTCT 4831385 2.795402 8.257519 20-24
GGTGT 4301600 2.7412007 9.873447 1
CGGTG 2923935 2.7370698 10.654519 9
TCAAG 3702625 2.7260623 5.5643334 10-14
TGGTG 4202565 2.6780906 9.848879 4
ATCGG 3201970 2.6582105 7.6938853 85-89
CAAGC 2453680 2.6536968 8.791725 30-34
TCGGA 3159050 2.6225793 8.023161 85-89
CTTCA 3804320 2.6050808 7.2170343 60-64
ACAAC 2715555 2.6046293 8.062992 85-89
AGAGC 2640580 2.594436 8.982615 95-97
TCTGC 3347515 2.584716 6.38229 85-89
GGTGG 3010690 2.5603216 8.234736 7
GCTGC 2484245 2.5597785 8.399148 3
CCGTG 2462110 2.5369704 6.0588675 60-64
CAACA 2604895 2.4984896 7.689447 85-89
CAACT 3073140 2.4905663 10.6331 9
TTCAA 4512430 2.4895387 10.621555 9
ACATC 3064670 2.483702 6.077516 90-94
TATGC 3975510 2.4731321 7.398903 30-34
GCAGC 2022285 2.4661636 5.7475977 1
AAGAG 3069560 2.429875 5.4196143 90-94
AGGAA 3062530 2.42431 6.572395 40-44
ACCCA 2024630 2.4102871 35.544918 1
ATGCA 3220640 2.3711998 6.666649 1
CGTGA 2816040 2.3378193 5.923208 9
GAGCA 2375560 2.3340473 6.4071126 90-94
GATCG 2803805 2.327662 6.823911 95-97
AGATC 3158255 2.3252687 7.1130753 95-97
CAGCA 2139315 2.313706 5.7630486 60-64
GCTGG 2465615 2.3080406 10.098072 2
CCAAG 2121730 2.2946875 7.5618744 25-29
TGAAA 3853190 2.2856545 5.6737733 65-69
GCACA 2101030 2.2723 6.8783803 95-97
AAGCT 3079815 2.2675176 5.652348 75-79
ACTGC 2419920 2.2113774 12.541158 2
GTTCC 2822410 2.179267 5.127173 70-74
CATGG 2619150 2.1743653 9.251763 2
GAAGC 2201065 2.1626015 7.3789225 15-19
CACAC 1802930 2.1463573 7.6597147 95-97
TCAAC 2645500 2.1439939 8.839615 8
CTTTA 4553835 2.1228244 5.9823303 5
TCCTT 3609690 2.088539 5.552435 40-44
CAGCT 2273365 2.0774522 5.087965 2
GGCTC 1995390 2.0560598 5.9757895 90-94
CTCTG 2653130 2.0485606 7.013346 20-24
CCAGC 1524255 2.0461001 6.8368793 1
CTTGG 2894295 2.030221 6.4097347 2
GCTAC 2204535 2.0145538 14.652541 1
TTCGG 2860905 2.0067995 7.9573674 7
CCTTA 2924870 2.0028608 7.411144 95-97
TGGCT 2806170 1.9684054 6.128295 9
CAAGG 1997690 1.9627805 7.0702677 10-14
TCGGT 2782710 1.9519491 8.872778 8
CTCTC 2287500 1.944201 5.358481 95-97
CATCC 1927900 1.9392602 7.152378 90-94
TGAAC 2618850 1.9281316 6.4712176 80-84
GTGGC 2048675 1.917747 7.449842 8
GCTTC 2475865 1.9116892 7.65016 15-19
TTCCA 2790720 1.9109991 5.7718544 25-29
CCTGT 2451130 1.8925906 7.490118 1
TTTCA 3950885 1.8417523 9.298213 8
TCTGG 2607065 1.8287417 5.2936325 45-49
AAGGA 2305030 1.8246703 6.1219683 10-14
CACAA 1900930 1.8232803 6.6477075 85-89
TTGGT 3816205 1.822321 16.91086 7
TATTC 3895930 1.8161342 5.0723147 5
GTGTA 3213195 1.8159415 8.68045 2
TGTAG 3208415 1.8132399 7.081174 3
GCATG 2183955 1.8130752 5.47964 3
CCGCC 1085595 1.8087268 6.1575184 75-79
TGCTG 2554720 1.792024 5.1166368 6
CACCC 1212130 1.791052 8.712244 50-54
GTCTG 2552985 1.7908069 6.184283 90-94
CTATG 2864085 1.7817239 5.5659175 35-39
CTCTA 2597985 1.7790201 6.511881 95-97
GGTAT 3111825 1.758652 5.032553 30-34
GCACC 1309850 1.7582914 8.103248 50-54
TGGTA 3089300 1.7459221 12.243537 2
GCTTG 2475615 1.7365353 6.159644 65-69
CATGC 1873715 1.712243 6.1285944 2
ACACG 1573775 1.7020646 6.8021655 95-97
AGCCT 1858115 1.6979873 5.7335973 4
GTAGC 2044380 1.6972028 6.233815 5
CCACA 1421630 1.6924261 7.28542 60-64
GCCTT 2191520 1.692138 5.0852256 35-39
CTTTC 2902300 1.6792483 11.084982 7
TACTT 3485785 1.6249404 9.763855 5
GAACA 1835420 1.5993114 6.192015 80-84
CCCAA 1340785 1.5961815 34.49281 2
ATGCC 1732805 1.5834762 7.0750585 9
ACACA 1644615 1.5774354 6.4885206 85-89
AGAAA 2243985 1.5753666 7.203725 9
CGCAG 1290380 1.5736102 6.1962066 95-97
GCTGA 1890385 1.5693593 5.7753906 80-84
CTGTA 2497480 1.5536618 5.569921 2
GAGCC 1273555 1.5530921 5.20792 95-97
ATCTC 2263720 1.5501258 6.608801 45-49
TCCAA 1894420 1.5352956 5.628801 25-29
GCAAG 1561640 1.5343505 5.1373096 25-29
AACAC 1599350 1.5340194 6.58714 85-89
CACGT 1655290 1.5126413 5.083101 95-97
GCAAC 1358620 1.4693708 7.157298 1
TGCAC 1599030 1.4612297 5.7559814 50-54
CCAAT 1797595 1.4568257 23.739021 3
ACGGT 1746645 1.4500291 6.995234 4
TTTAG 3423290 1.4497421 5.2471695 6
GTATT 3421850 1.4491322 5.4774647 4
ACTTT 3097940 1.4441417 9.783374 6
GGTAC 1721550 1.4291959 15.570452 3
GGTCA 1682005 1.3963666 24.344799 9
ACATG 1894960 1.3951666 7.924097 1
ATGGG 1838055 1.3862476 6.708495 3
GGTCG 1454660 1.3616947 6.0036063 6
AAGCC 1248970 1.3507825 5.4425573 3
GACTG 1613110 1.3391712 9.797902 1
GACGG 1202395 1.3321002 8.944678 3
TGGTC 1891980 1.3271413 20.785215 8
GCTGT 1880645 1.31919 5.2700005 7
GTCAA 1777495 1.3086827 5.0837865 10-14
TGGGT 2040495 1.3003083 5.0622697 8
TGTAT 3050915 1.2920436 5.190873 3
ACGCA 1177965 1.2739893 5.1340065 95-97
AACGC 1153340 1.247357 5.661042 95-97
GGGTC 1322115 1.2376202 7.02965 9
ATTCG 1965605 1.2227868 6.2584896 6
GTAGA 1798565 1.202991 5.4018393 4
GTACT 1877315 1.1678622 11.703843 4
CAATT 2064640 1.1390761 16.434658 4
TGCCT 1472115 1.1366638 5.015184 30-34
CTGAC 1242640 1.1355524 7.9343014 1
TGACG 1333380 1.106945 6.7295427 2
CTTAT 2362310 1.1012189 5.069454 95-97
ATTGG 1934425 1.0932428 16.643244 6
ACTTG 1674410 1.0416368 6.416224 8
TGGCC 965230 0.9945779 7.982946 1
TAGAA 1614665 0.95779496 5.7764616 8
CGGTA 1140170 0.946546 6.573073 5
AATTG 1837550 0.9209961 13.877603 5
ACGCG 675890 0.82424355 5.334473 15-19
TTAGA 1594930 0.79939276 5.2479134 7
CTTGT 1206995 0.6344368 5.3031363 9
TGACC 629620 0.57536095 6.026259 1
Produced by FastQC (version 0.10.1)