FastQCFastQC Report
Thu 31 Jul 2014
1S15B_GATCAG_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1S15B_GATCAG_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11140319
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 255718 2.2954279855002357 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 128355 1.1521662889545623 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 116740 1.047905360699276 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 107362 0.96372464738218 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 100143 0.8989239895195101 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 65435 0.587370972052057 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 64223 0.5764915708428098 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 63767 0.5723983307838851 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 63631 0.5711775398891181 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 63234 0.567613907644835 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 59866 0.5373813801920753 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 51220 0.45977139433798975 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 45705 0.4102665282744596 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 45526 0.40865975202325894 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 44927 0.40328288624410125 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 43113 0.38699969004478235 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 41469 0.3722424824639223 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 41326 0.3709588567436893 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 40439 0.3629967867167897 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 39758 0.35688385583931664 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 36943 0.33161527959836695 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 36847 0.3307535448491197 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 34186 0.30686733476842093 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 33685 0.30237015654578653 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 32129 0.28840287248506974 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 31723 0.2847584526080447 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 30172 0.2708360505655179 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 29405 0.26395114897517746 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 27544 0.2472460618048729 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 26060 0.23392507880609162 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 25639 0.23014601287449668 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 25119 0.22547828298274045 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 25016 0.22455371340802718 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 24991 0.22432930331707737 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 23006 0.20651114209566174 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 22945 0.20596358147374413 No Hit
CGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTC 20824 0.18692462935756146 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 20696 0.18577564969189841 No Hit
GATGAAATTAGCAGTATTATCCGTAAACAGATAGAAGACTATAGTCAAGA 20505 0.18406115659704178 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 20415 0.18325328026962243 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 20042 0.17990508171265113 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 19763 0.17740066509765115 No Hit
GGTCCTTACGAGTTAATCGTTCTTCACTTCTTACTTGGTGTAGCTTGCTA 19268 0.17295734529684473 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 17506 0.15714092208670147 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 17120 0.15367603028243626 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 16626 0.14924168688526782 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 16413 0.14732971291037536 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 15840 0.1421862336258055 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 15308 0.13741078689039335 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 15160 0.1360822791519704 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 15158 0.13606432634469443 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 14933 0.13404463552614607 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 14825 0.13307518393324286 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 14768 0.13256352892587728 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 14694 0.1318992750566658 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 14456 0.1297628909908235 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 14337 0.12869469895790236 No Hit
TAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTA 14141 0.12693532384485579 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 13858 0.12439500161530383 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 13837 0.12420649713890598 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 13510 0.12127121314928235 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 13142 0.11796789661050103 No Hit
GGCCCTGGAGATTTCTTAGTTCATCACGCTATTGCTTTAGGTTTACATAC 13068 0.11730364274128953 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 12863 0.11546347999550104 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 12386 0.11118173546017848 No Hit
TGGCCCTGGAGATTTCTTAGTTCATCACGCTATTGCTTTAGGTTTACATA 12130 0.10888377612885232 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 11874 0.10658581679752618 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 11855 0.10641526512840431 No Hit
GCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTC 11759 0.105553530379157 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
ACTTC 3998610 3.7120845 7.5966167 60-64
CTGCT 3569290 3.5184002 15.678098 5
AACTT 4438305 3.3885436 6.414636 60-64
AAAAA 3515560 3.2119684 7.191633 45-49
CAACT 2814960 3.152302 9.336778 55-59
CTACT 3192245 2.9635003 16.217443 2
GGAAG 2684690 2.936532 7.4525695 15-19
TTCAA 3842385 2.9335723 8.989202 9
TCAAG 2973590 2.9078932 6.5814962 10-14
CTTCA 3127240 2.9031534 7.815602 60-64
TCAAC 2574775 2.8833334 9.037045 55-59
AGCTG 2770505 2.876808 8.947121 3
AGGAA 2746825 2.8295472 8.179095 40-44
TCTCT 3666515 2.8217297 9.392875 20-24
CTGGT 3224085 2.775311 13.988114 1
CTTCT 3577345 2.753105 7.5173965 40-44
ACCCA 1651200 2.712158 48.054806 1
ACTGC 2187175 2.600721 16.907476 4
AGCAC 1812610 2.5999289 6.4918375 35-39
ACAAC 1917045 2.5896122 8.708751 85-89
GCTGC 2015130 2.5442977 9.062107 3
GAAGC 2020080 2.5302732 8.630163 15-19
AAGCT 2570420 2.5136309 6.5952005 15-19
CAAGC 1712730 2.4566653 9.29825 75-79
CAACA 1811315 2.4467883 8.610619 85-89
GGTTC 2820695 2.4280705 6.4561996 3
CGGTG 2188170 2.412613 16.190523 9
CAAGG 1925320 2.4115803 8.095429 10-14
TATGC 2972530 2.4097767 8.344858 30-34
CAGCA 1679985 2.4096973 7.373299 35-39
GCAGC 1551795 2.363448 9.463137 1
CTGAA 2408025 2.3548234 7.012514 80-84
CTGCA 1975800 2.3493798 6.5753174 95-97
GGTGT 3113880 2.3407161 13.53071 1
CTCTG 2368470 2.334701 9.909635 45-49
ACATC 2061550 2.308604 7.0133677 90-94
GGTGG 2360290 2.2725513 9.738346 7
CCAAG 1582310 2.2695966 9.0726185 75-79
TGGTG 2975520 2.2367108 8.324071 6
GCTAC 1843075 2.1915596 20.838556 1
ATGGT 3089665 2.1872764 7.662309 1
CATGG 2106350 2.1871698 13.770746 2
TCTGC 2212145 2.180605 5.5493355 85-89
CCGTG 1698960 2.1451023 6.451034 60-64
TCCTT 2786465 2.144448 6.8654237 40-44
CGTGA 2043105 2.121498 11.986214 9
GTTCC 2138070 2.1075861 5.816985 70-74
CAGCT 1764720 2.0983891 6.8116837 2
ATGCA 2138410 2.0911653 5.9139137 1
TTTCA 3297885 2.0872984 7.5170646 8
GGTAT 2930430 2.0745487 6.3838816 30-34
CCCAA 1258350 2.0668871 48.711716 2
AAGGA 2002190 2.0624874 7.2223845 10-14
GCTCC 1395095 2.0170975 7.048961 70-74
CGGAA 1602685 2.0074606 5.7701483 95-97
CTCCT 1778270 2.0073326 8.593146 70-74
TGCAG 1919700 1.9933581 5.451152 65-69
GCTTC 1994405 1.9659694 9.385286 15-19
CTTTA 3102225 1.9634613 5.009085 35-39
TTCCA 2109700 1.9585265 6.5173354 75-79
GCTGG 1768410 1.9497977 10.500262 4
CCTTA 2089940 1.9401824 9.051482 95-97
GATGG 2139375 1.9399058 5.1364074 1
CACAA 1431370 1.9335455 8.311243 85-89
CCAGC 1107685 1.9319067 6.833226 1
CCAAT 1714165 1.919589 33.484715 3
CTTGG 2225800 1.9159812 7.418653 2
CATCC 1404900 1.9129951 8.323679 90-94
TGCTG 2210805 1.9030734 12.370811 6
TCTGG 2209685 1.9021093 5.5342174 45-49
CTTTC 2467050 1.898628 8.877692 7
ATCCG 1581850 1.8809427 5.9324307 95-97
AGCTC 1579070 1.8776368 5.798157 70-74
CTCTA 2016230 1.8717543 6.2316604 25-29
TGGCT 2174250 1.871607 7.0386953 9
CTGTT 2759940 1.8548266 9.858557 8
CCTGT 1880385 1.8535751 10.153589 4
CTCTC 1621295 1.8301373 5.231452 3
AATGA 2270125 1.8257205 6.726327 75-79
GCAGG 1359330 1.8079163 6.0383 90-94
GGCTC 1423860 1.7977616 6.351462 1
TACTG 2204815 1.7874038 11.615098 3
TTCGG 2054370 1.7684133 12.524362 7
TAGCT 2178780 1.7662977 6.2537374 2
GCCTT 1787755 1.7622658 5.9343534 35-39
ATGAT 2627885 1.7520401 5.350941 15-19
GAAAA 1805515 1.75159 5.6402755 40-44
ACGGT 1685385 1.7500526 7.998855 4
TGGTA 2462470 1.7432643 10.734166 2
TATTC 2728290 1.7267904 7.863428 5
CACCC 863765 1.7251376 7.6498566 50-54
TCGGT 2001535 1.7229328 14.939443 8
GAACA 1457850 1.7197173 7.5190744 80-84
CCTAC 1254900 1.7087462 5.4153852 80-84
GGAGA 1548025 1.6932402 5.089903 7
GCACC 962070 1.6779405 6.886538 50-54
AGCTT 2069255 1.6775078 5.481315 15-19
ATGCC 1403900 1.6693462 8.367866 9
GCAAC 1161975 1.6666863 6.846964 1
CTGTA 2051820 1.6633736 7.9405336 5
GTGGC 1507215 1.6618117 8.34752 8
TTGGT 2820335 1.6551834 17.930134 7
ACCAG 1145230 1.642668 5.808998 6
GCATG 1577275 1.6377943 5.1132874 3
TTAGG 2313200 1.6375911 5.9862905 35-39
TGTAG 2310620 1.6357646 7.4052787 6
ACACA 1210470 1.6351457 8.054055 85-89
GGTAC 1569260 1.629472 12.969199 3
CTACC 1195780 1.6282449 7.141433 20-24
TACTT 2564820 1.6233269 7.975408 5
CCTCC 975670 1.6154156 7.1137586 1
AGATC 1645020 1.6086758 5.3382025 95-97
TGAAC 1642490 1.6062018 6.6617365 80-84
GACGG 1203315 1.6004155 10.254434 3
CCGCC 751345 1.5933903 8.308829 45-49
GCTGA 1529170 1.5878437 6.558578 80-84
CATGA 1613915 1.578258 5.9487977 65-69
GCTGT 1829855 1.5751498 12.547312 7
GTCAA 1610320 1.5747426 6.1672974 10-14
TCCAA 1405205 1.5736034 6.959308 75-79
CGCAG 1026695 1.5636989 6.808044 90-94
CCACA 947950 1.5570434 6.5834036 60-64
AACAC 1151705 1.5557641 8.087596 85-89
TTCAT 2444840 1.5473891 6.19759 60-64
GCACT 1294160 1.5388567 5.7658854 35-39
AACAT 1662830 1.5314069 5.6615553 90-94
ATTCC 1643720 1.5259372 5.396944 80-84
CAACC 926145 1.521228 5.5491424 75-79
ATCTC 1623635 1.5072914 6.6762114 45-49
GTGTA 2114450 1.4968894 12.3660145 2
TCATG 1844735 1.4954934 5.0574193 60-64
GTGTT 2542565 1.4921674 5.086664 70-74
GGTCA 1427810 1.4825945 28.357407 9
GGAAT 1722615 1.4710498 5.4968524 40-44
GCTTG 1706785 1.4692101 7.1928973 1
GTAGC 1395825 1.4493822 7.7886744 1
AACCA 1061265 1.4335945 6.197063 75-79
GAATC 1459660 1.4274111 5.9199057 40-44
ACTTT 2246415 1.4218019 7.8600607 6
CACAC 858745 1.410521 5.65806 95-97
TGGTC 1630630 1.4036556 23.714758 8
ACATG 1434795 1.4030955 12.560251 1
AGGTA 1639545 1.4001111 7.1739845 6
GGCTG 1266335 1.3962244 5.9959555 9
GCAAG 1113030 1.3941379 5.403524 1
GCGGC 860530 1.3916574 7.7332325 7
TTCTC 1805605 1.3895836 5.887465 20-24
TGTTT 3023085 1.3851422 7.2707725 9
CTGAC 1160045 1.3793837 8.921422 1
GTACT 1689415 1.3695784 9.957595 4
CAATT 1788900 1.3657838 22.641737 4
GTATT 2468870 1.3645488 7.330272 4
CAGGT 1302700 1.3526841 5.687895 95-97
AGCCT 1132260 1.3463451 7.4653563 2
GCCTG 1066020 1.345954 8.94828 6
TGCCT 1358835 1.3394612 5.819056 30-34
GTAAC 1366185 1.3360013 5.017009 8
GTATG 1886825 1.335746 5.7255363 7
ATGGG 1469540 1.3325242 13.010162 3
GTCTG 1545855 1.3306807 5.4942746 90-94
TGACG 1281155 1.3303125 7.796225 2
AACGC 894775 1.2834263 5.1814346 90-94
ACCGC 726345 1.266814 5.615373 45-49
GGTCG 1141870 1.2589928 12.233481 6
TAGGT 1776250 1.2574663 5.544002 5
TAGGA 1441255 1.2307787 5.118145 40-44
ATCCT 1316330 1.2220066 5.6655025 90-94
TGTAT 2186810 1.2086537 7.2118564 3
AAGCC 835990 1.1991076 8.307863 1
ATTGG 1675600 1.1862129 20.058765 6
TGGGT 1568590 1.1791154 8.977381 4
CGGTA 1116810 1.1596615 7.456773 5
TGGCC 908635 1.14724 13.332746 1
GGGTC 1026780 1.132098 12.230799 5
TACCC 819545 1.115941 5.3130155 20-24
ATTCG 1365140 1.1066945 9.980395 6
GCGAC 722585 1.1005268 6.1281166 20-24
GCTCA 910185 1.0822808 6.346505 1
AATTG 1601895 1.0680013 18.294498 5
GTAGA 1241545 1.0602337 5.395282 4
CGCCT 722890 1.0451901 5.3801093 45-49
TCGTG 1203330 1.0358332 9.495082 8
CTTAT 1623010 1.0272361 6.203903 95-97
CCAGG 672385 1.02407 5.275346 2
TGCGG 912870 1.006504 5.5683985 6
GTTCG 1168935 1.006226 6.0952735 4
TGGGG 1036235 0.9977152 6.7584105 8
TTGGC 1146350 0.9867846 6.0965247 3
CGGCT 769035 0.97098136 5.8031316 8
TTATG 1743635 0.96371 5.514793 95-97
AACGG 762970 0.95566636 5.9852033 60-64
AGTAG 1085200 0.9267208 5.904846 9
GGGGT 956680 0.92111754 6.4107122 9
GGCCA 587280 0.8944517 5.4946294 1
GCCCA 501005 0.87379986 5.946311 2
GGCCT 678250 0.8563565 8.677149 5
GGCCC 427820 0.7922926 9.0413685 1
CCCTA 567695 0.7730072 7.220764 2
GCCCT 520465 0.75251406 8.379338 1
GCCTA 631860 0.7513307 7.2404623 3
CACGG 473695 0.72145694 5.6129856 4
GTCGT 834005 0.7179163 9.361144 7
CCTAT 683990 0.63497776 5.4773183 4
GGTAA 701675 0.59920466 7.3486676 7
CCTAG 330480 0.39296642 6.0686855 3
CTAGG 377315 0.39179242 5.456556 4