FastQCFastQC Report
Thu 31 Jul 2014
1S15A_GATCAG_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1S15A_GATCAG_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14316109
Filtered Sequences 0
Sequence length 101
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 221876 1.549834525568365 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 217788 1.521279280564293 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 169911 1.1868518184654782 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 136544 0.9537787117994143 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 115361 0.805812529088735 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 91988 0.6425488936973028 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 89590 0.6257985322687889 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 78329 0.5471388908815936 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 71497 0.49941642662821295 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 71207 0.49739073654720006 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 61441 0.42917387678453695 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 57003 0.39817383340682866 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 56579 0.3952121348056235 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 55418 0.3871023893433614 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 54393 0.3799426226777122 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 51601 0.36044011679430493 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 49623 0.34662351341415465 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 48841 0.3411611353336301 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 47629 0.3326951478226381 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 45122 0.31518340632919184 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 44465 0.31059417052496596 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 43233 0.3019884802497662 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 42851 0.2993201574533974 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 40365 0.2819551038623693 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 37932 0.26496026259649186 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 34462 0.2407218330064405 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 33437 0.23356206634079132 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 32576 0.22754786234164603 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 31384 0.21922157759486183 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 31183 0.21781756481457354 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 30562 0.21347979398592173 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 30010 0.20962399769378676 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 26988 0.1885149100219899 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 25043 0.17492881620278247 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 24220 0.16918004745563198 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 23832 0.1664698138300009 No Hit
AGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 22955 0.16034384761948933 No Hit
GGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 22891 0.1598967987740244 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 22718 0.15868836986362705 No Hit
TGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 22580 0.15772442079059332 No Hit
TGACGAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21375 0.14930732924707404 No Hit
TGGCTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 21206 0.14812684088951822 No Hit
GCAGGAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 20345 0.14211263689037293 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 20323 0.14195896384974438 No Hit
TTGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 19154 0.13379333728179912 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 19016 0.1328293882087654 No Hit
CTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGA 18775 0.13114596990006153 No Hit
GCAGATGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 18055 0.12611667038858113 No Hit
GCAGTAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 17538 0.1225053539338098 No Hit
GCAGAGGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 17299 0.12083590590152672 No Hit
CTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCA 17227 0.1203329759503787 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 16318 0.1139834853171347 No Hit
GCTGCTGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 16220 0.11329894177251655 No Hit
GGCCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 15949 0.11140596931750102 No Hit
CGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 15894 0.11102178671592959 No Hit
GCAGCAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 15447 0.10789942993588553 No Hit
TGGTCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15153 0.10584579930203102 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 14139355 7.2752542 12.486389 7
AGCAG 7046460 5.778243 68.66247 20-24
CACGG 4587945 5.3052716 54.770557 45-49
TAGCA 7951345 5.0321217 42.054993 20-24
GCAGA 5803935 4.759347 44.35839 25-29
ACTTG 6497605 4.6920156 118.16233 8
CCTGC 3690495 4.6443434 60.536163 15-19
CTGCG 3321675 4.382704 62.712708 15-19
TCACG 4838295 4.3178496 42.29006 45-49
GTTTC 5191560 4.2775955 44.528316 40-44
CGGAT 4395900 4.1130905 41.370182 45-49
GAACC 5056870 3.9551353 36.80027 70-74
GAAGT 5818485 3.8606975 35.846123 7
AGAGA 6397215 3.7200708 25.72855 90-94
ACGGA 4492550 3.6839843 38.28799 45-49
AGTAG 5306330 3.5208707 35.838314 9
GCGTT 3258990 3.4793575 47.536285 15-19
CTTGA 4798885 3.465345 34.602238 55-59
GCAGC 2993055 3.46102 26.40224 20-24
AACTT 6909110 3.3745701 80.05801 7
TGCGT 3147110 3.3599124 50.237125 15-19
GAGCC 2808725 3.2478695 80.027596 95-97
ACCTG 3619270 3.2299528 33.10839 15-19
TTCTC 4095660 3.218699 35.663754 30-34
CTTGT 3881920 3.1985147 134.92865 9
CCATG 3574690 3.1901681 42.324577 75-79
AGGAG 3704545 3.1849673 27.137121 90-94
ATAGC 5023440 3.1791556 29.679575 80-84
ACCAT 5241185 3.163696 37.547184 75-79
AACCA 5951205 3.1482892 23.754816 70-74
CATGC 3518285 3.13983 42.06633 75-79
AGAGC 3674945 3.013531 61.88626 95-97
CACTG 3354590 2.9937437 41.45485 85-89
CTCAG 3320420 2.9632494 42.3128 35-39
TTCAC 4249995 2.927179 26.355028 45-49
ACAGG 3541585 2.9041731 17.848017 90-94
GCACT 3214260 2.8685088 31.639317 85-89
TGCAT 3972160 2.868355 32.24023 75-79
AGAAG 4927230 2.8652537 23.30298 6
GAGAG 3314510 2.8496363 49.97219 95-97
GACTC 3191715 2.8483887 41.884098 25-29
AGCAC 3599010 2.8148975 34.34569 80-84
GCTGC 2109980 2.7839622 42.67369 1
TGAAG 4156650 2.7580323 24.357128 60-64
AAGTT 5365750 2.7477174 25.246283 60-64
GGAAC 3330970 2.7314644 38.759697 70-74
TTTCA 4846910 2.701201 25.478071 40-44
TTGAA 5159475 2.6420867 23.159714 55-59
CAGAC 3327945 2.6028893 36.89331 25-29
GGAGG 2039165 2.5919843 24.13981 90-94
GGATT 3415440 2.585819 27.537676 50-54
TTAGC 3536025 2.5534153 34.574375 20-24
AAACT 5877790 2.5160244 41.356396 6
GTAGT 3311080 2.5068085 27.534275 40-44
GTTAC 3468965 2.5049903 31.93637 60-64
TCAGT 3467410 2.5038674 34.503124 35-39
GGCTC 1891990 2.4963405 27.143648 65-69
CATTC 3618965 2.4925578 32.930603 30-34
TTGTA 4251800 2.4843342 33.434475 9
ATGCA 3917850 2.479467 30.072546 75-79
TCTCA 3593740 2.4751842 25.243664 35-39
GATGA 3718605 2.467379 12.547412 15-19
GTTAG 3241800 2.4543564 36.209282 20-24
CAGTA 3838870 2.4294837 29.863295 35-39
GTTGT 2810200 2.4276402 17.84557 35-39
CAGGC 2086850 2.4131296 20.669308 65-69
CTCAT 3501845 2.4118917 25.113298 30-34
CTGAA 3788925 2.3978753 29.285236 85-89
TAACC 3971110 2.3970504 28.392502 10-14
AGTTT 4078555 2.383107 28.165411 40-44
ACCAG 2989650 2.3382983 16.993107 55-59
CATAG 3680735 2.3294055 29.761057 80-84
AAGGA 3998500 2.3251846 21.276564 70-74
CGTTA 3212605 2.3198693 26.06252 20-24
TAGAG 3478875 2.3083127 30.356739 90-94
GCATA 3646420 2.307689 22.905794 80-84
AGGAA 3940060 2.2912004 27.672817 70-74
AACCT 3769930 2.275614 27.147081 10-14
CAGAA 4084855 2.265644 17.233545 5
CAGCA 2894535 2.263906 20.76515 20-24
GGAGC 1861805 2.257194 26.748531 95-97
TGTTG 2607585 2.2526076 15.94759 35-39
GTAGG 2293890 2.250289 26.961048 7
GTAAC 3549030 2.2460544 30.127043 10-14
CCATC 2631715 2.2401068 17.896906 75-79
CTAAG 3539260 2.2398713 29.653412 65-69
TGTAA 4352935 2.2290702 24.740437 10-14
TTTCC 2826765 2.2214997 15.195281 40-44
CTGCA 2461795 2.1969848 24.335785 3
TCATT 3935750 2.193408 26.608522 30-34
TAAGG 3301270 2.190468 29.414967 65-69
AGACT 3379895 2.1390147 30.074846 25-29
CCAGA 2722310 2.1292033 21.242579 55-59
ATTCT 3806320 2.1212761 26.668352 30-34
ACTGA 3349855 2.1200035 29.706697 85-89
GAAGA 3585365 2.0849402 11.073962 50-54
TTCCG 2044430 2.081819 20.360743 40-44
ACTCA 3440415 2.0767112 26.974611 25-29
TAGTT 3540295 2.0686007 27.7558 40-44
AGTTA 4029270 2.0633264 24.023748 60-64
TGAAT 3978965 2.0375664 23.078735 85-89
AGGGA 2348650 2.019242 23.791563 9
TGCAG 2157300 2.0185103 28.199549 4
GGATG 2031630 1.9930139 20.748419 10-14
TTACT 3542325 1.9741509 20.196754 65-69
TTAAA 5634545 1.9515992 16.93774 50-54
TCCGT 1911185 1.9461374 20.975237 40-44
TAGGG 1966885 1.9294994 26.946304 8
TTGTT 2889140 1.9262003 14.484316 35-39
GATGT 2537665 1.9212581 19.549597 30-34
GAATA 4259425 1.9115999 16.287636 90-94
ATGAT 3706815 1.8982023 10.7857685 15-19
AAGAG 3246855 1.8880922 12.22716 50-54
AATAG 4201245 1.8854891 21.275341 90-94
CAGGA 2296560 1.8832269 16.604622 90-94
CAGAG 2280410 1.8699836 16.668583 25-29
TGATG 2465095 1.8663158 15.987851 30-34
AGGCT 1968245 1.8416181 18.638182 65-69
GCTCA 2060220 1.8386065 22.904966 65-69
GAGGA 2127280 1.8289201 24.053974 95-97
CCAAA 3435660 1.8175226 35.26845 4
TGACC 2011565 1.7951852 58.36463 1
TACTA 3660780 1.7880101 23.149815 65-69
TGTTT 2670680 1.7805521 17.601875 35-39
ATTAA 5140080 1.7803347 16.866348 50-54
GGGAT 1811490 1.7770581 25.103294 10-14
AGCTG 1893925 1.7720795 10.579951 95-97
TAAAC 4138305 1.7714267 19.40107 50-54
CTCAC 2070365 1.7622875 16.823984 65-69
TACAG 2760695 1.7471452 12.820165 90-94
CTACA 2888250 1.743412 13.31944 85-89
GATTA 3377155 1.7293885 24.196243 50-54
CATCA 2831715 1.7092862 11.17756 80-84
TCCTC 1737070 1.6871077 10.59034 1
TACCA 2771735 1.673081 12.669963 75-79
ATACC 2770660 1.6724321 12.145659 75-79
AAGAA 4234285 1.6654459 11.035518 45-49
GAGCT 1758840 1.6456851 13.06913 95-97
GAGAT 2470680 1.6393526 17.219326 25-29
AACAG 2936615 1.6287786 16.698566 60-64
ACCAA 3075210 1.6268384 18.646124 3
CCTCC 1354060 1.6252955 5.968335 1
ATAGA 3604890 1.6178491 21.119387 90-94
ACTAA 3770960 1.6141825 20.463932 65-69
TCTAC 2320370 1.598152 17.240599 85-89
CCGTA 1772355 1.5817065 22.714336 40-44
GAAAC 2796000 1.550787 11.325888 60-64
GGAAG 1793415 1.5418812 10.873123 6
GAGAA 2630195 1.5294955 15.316058 50-54
GGGGG 798205 1.500045 7.23418 1
TAAAG 3333180 1.4959075 10.089635 45-49
GTAAA 3320605 1.4902642 9.272698 45-49
TCTCC 1532540 1.4884604 5.0932226 1
ATGTT 2533250 1.4801825 11.742195 30-34
AGCCA 1875785 1.4671098 15.267894 95-97
CAAAC 2770530 1.4656577 40.975338 5
GACCA 1870865 1.4632617 24.78769 2
GATAC 2303890 1.4580495 11.151236 75-79
AGATG 2186020 1.4504741 13.262931 25-29
TGCTG 1358270 1.450114 8.076 1
AAAGA 3577430 1.407089 8.783275 45-49
AGAAA 3556050 1.3986797 7.7458396 60-64
GGCCA 1184085 1.3692169 22.388138 2
AGAAC 2427845 1.3465917 12.17382 50-54
AAGTA 2973575 1.3345195 25.070818 8
CTCTT 1685700 1.3247588 7.456444 3
GCTCC 1050730 1.3223026 5.9460278 1
TCAAT 2690400 1.3140539 10.069666 80-84
CTGCT 1281830 1.3052726 5.0871186 2
GAGCG 1074455 1.3026354 6.7084846 90-94
CGTAA 2046400 1.2950934 12.5535 40-44
CAATA 3010480 1.2886544 11.0197525 80-84
GATAG 1936335 1.2848023 17.037552 15-19
TCTTG 1555550 1.2816981 6.814374 4
GCCAA 1638160 1.281256 17.55797 3
GCTGG 910610 1.2596873 6.9663615 1
TCCAA 2065600 1.2468423 5.3221507 3
CAACA 2336410 1.2360008 7.0386705 8
AAACA 3291930 1.2349681 8.207979 60-64
TATCT 2215210 1.2345448 11.014851 85-89
TGATA 2399020 1.228501 10.879644 15-19
GGATA 1849960 1.2274907 12.953483 70-74
ATCAA 2867120 1.2272882 8.7753 80-84
ATCTA 2462180 1.2025859 10.318687 85-89
CCTCT 1207955 1.1732112 8.744742 2
TGGCC 885790 1.1687343 38.95066 1
GCAGT 1241280 1.1614225 5.0092077 4
CTTTT 1771535 1.1265156 5.304778 6
CCGAA 1424110 1.1138408 35.261253 4
CCCAA 1451500 1.0828077 8.318693 3
GCCCA 979570 1.0803876 7.793724 2
CCAAC 1446595 1.0791485 5.4825907 5
CGGAA 1315850 1.0790242 9.316671 5
TGTAG 1410290 1.0677261 6.0266485 3
CCTTT 1324565 1.0409498 5.1498156 4
ATATC 2118900 1.03492 8.699968 85-89
TCAAC 1709430 1.03185 8.144025 7
AATAT 2975720 1.03068 7.000793 80-84
CCATA 1684660 1.0168984 5.3034077 3
TGCCA 1133690 1.0117413 5.5300374 1
TTGAC 1384355 0.999663 6.4611297 6
GTTGC 911605 0.9732462 7.696264 1
CGAAC 1209475 0.94596803 33.585308 5
GAACT 1463835 0.9264089 29.439976 6
GGTGC 663030 0.91719884 9.677336 2
CTCCG 722500 0.9092379 5.5237584 2
ACCTA 1505640 0.9088379 8.164653 3
CCTAA 1493770 0.90167296 11.108879 4
TAAGT 1756190 0.8993176 5.6257644 5
GACCT 1007110 0.89877725 11.947153 2
GTCCA 998085 0.8907231 5.8768067 2
CTAAA 2039650 0.87308466 6.644866 4
GGCTG 631085 0.8730079 10.997964 1
ATTGG 1151650 0.8719106 5.169376 6
GTGCA 931170 0.8712633 7.310086 3
ACCGA 1113840 0.871169 21.830017 3
TCCCC 715755 0.8591299 5.1884384 1
TGTCC 836815 0.8521189 9.913043 1
GCTGT 790320 0.84376013 9.888683 1
TGCCC 659670 0.83016884 8.918736 1
GGGTG 567520 0.82310784 10.908607 1
CTGTC 805940 0.82067925 10.51083 4
CCCCC 546095 0.81008685 5.420264 1
AACAA 2094985 0.7859339 5.4016223 9
TGGCT 730320 0.779703 8.027821 1
GGCCT 586505 0.7738499 7.5325346 2
GCAGG 638095 0.7736064 6.132998 1
TGTCA 1059220 0.76487833 8.07329 5
AAGTC 1192150 0.7544692 6.835777 6
GGGCC 439780 0.7518561 10.797888 1
GGGGT 513410 0.7446289 5.190386 1
GTCAA 1160785 0.7346193 8.158176 6
GACCG 626520 0.7244765 31.89802 2
CCGTC 574710 0.72325003 5.9753804 4
GCCAG 623970 0.7215279 5.3796387 2
AGTCT 996850 0.71983993 9.098244 7
GTAGA 1065690 0.70710963 5.3942165 4
GGGGC 391655 0.70201796 5.215167 1
GGCCG 402630 0.68834376 21.170025 2
TCTAA 1325770 0.64753693 6.5440893 9
CTAAC 1059285 0.63940805 10.16102 5
TGCGG 460545 0.63709235 15.107122 3
TCCGA 709290 0.63299316 5.0360203 3
GCCCC 397280 0.6178818 6.239053 1
GCCGA 533825 0.61728865 14.996282 3
TGACT 841550 0.60769564 13.545192 1
CAGGG 493665 0.598504 5.3726196 2
TAACT 1219940 0.595847 8.5605345 6
GGCCC 363060 0.59201455 7.057987 1
GTCTA 815875 0.5891553 9.11436 8
GACTG 628910 0.5884491 9.19073 2
CCCCG 374185 0.5819626 5.764154 2
GACCC 523950 0.577875 6.7722187 2
GACTT 779130 0.5626212 8.920611 6
CTGTA 775180 0.55976886 5.4951696 2
GCGGA 439415 0.53273296 12.367092 4
GCCCG 301180 0.49111155 6.3606343 2
CCCGA 443605 0.48926097 5.566702 3
GTCCG 348865 0.46030152 6.3642783 2
GGACC 396635 0.45864895 5.811462 1
CCCGT 364160 0.4582811 5.536642 3
GACTA 574835 0.36379254 5.918892 2
Produced by FastQC (version 0.10.1)