FastQCFastQC Report
Wed 6 Aug 2014
1L22C_ATCACG_L004_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L22C_ATCACG_L004_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 15250115
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 284128 1.8631203764692923 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 252410 1.6551350596372552 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 138039 0.9051669446427124 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 118860 0.7794039585930991 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 105257 0.6902046312437644 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 89049 0.5839234654951783 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 77230 0.5064224105851005 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 73391 0.4812488299268563 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 73307 0.4806980144084159 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 59800 0.39212819050872727 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 56933 0.37332833227815004 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 53007 0.347584264118664 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 52432 0.3438138007483878 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 40834 0.26776191523801623 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 38680 0.2536374315865815 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 37570 0.24635879795004825 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 36780 0.24117850914566874 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 34849 0.2285163095491411 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 34137 0.22384749229759907 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 32600 0.2137688797756607 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 31828 0.20870662286808983 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 30819 0.20209027931920512 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 30754 0.2016640530251739 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 29253 0.19182150429685285 No Hit
GCTGCTGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 27211 0.17843144133667188 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 26884 0.1762871952113148 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 26636 0.17466097796639565 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 26174 0.17163149261497374 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 25463 0.16696923269103217 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 24459 0.16038567578014984 No Hit
CGTCCCATATTTAAGTCGTCTGCAAAGGATTCATCTCCCCGATCGTCTGG 24254 0.15904142362205137 No Hit
CGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 24102 0.15804470982677835 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 22846 0.149808706360575 No Hit
GCTGCCGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 22496 0.14751364170040687 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21090 0.13829403909413143 No Hit
GCAGAGGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 19673 0.1290023058842507 No Hit
GCAGATGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 18648 0.1222810450937583 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 18310 0.12006466836479593 No Hit
GCAGTAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 17844 0.11700895370297208 No Hit
CGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 17668 0.11585486404528753 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 17308 0.11349422610911458 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 16855 0.11052375670609699 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 16415 0.10763853256188559 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 16381 0.10741558342346927 No Hit
ACAGGGGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 16158 0.10595329936856214 No Hit
GCTGTGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 16067 0.10535658255691842 No Hit
GACCACACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 15810 0.10367134936359497 No Hit
GCAGACGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 15382 0.10086481315058936 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AGCAG 6468000 4.8303723 54.821987 20-24
TTTTT 8533975 4.5612416 7.935687 7
TAGCA 7558605 4.498467 35.795048 20-24
ACTTG 6417630 4.4002376 111.19589 8
CACGG 4302390 4.359728 45.665928 45-49
CCTGC 3694560 4.048216 50.201862 15-19
GCAGA 5309180 3.9649532 36.970783 25-29
GTTTC 4991725 3.9430342 39.332767 40-44
CTGCG 3241355 3.784031 52.822643 15-19
TCACG 4518340 3.648723 36.4179 45-49
CGGAT 4092910 3.5214481 36.180855 45-49
GAAGT 5473765 3.4708526 28.784573 60-64
GAACC 4836295 3.3899732 31.678593 70-74
AGAGC 4509345 3.3676274 53.45173 95-97
CTTGA 4861730 3.3334372 31.557898 55-59
AGAGA 5945000 3.2720761 22.551992 90-94
AACTT 6890000 3.2677925 77.04293 7
AGTAG 5055030 3.2053375 27.667835 9
ACGGA 4187850 3.1275318 33.271538 45-49
GCGTT 3119220 3.0918157 42.055676 15-19
TTCTC 4163835 3.0870683 32.162197 30-34
CTTGT 3870090 3.0570388 128.22054 9
CCATG 3774765 3.048259 37.086033 75-79
TGCGT 3071845 3.0448565 44.580524 15-19
ACCTG 3635095 2.93547 29.722132 15-19
CATGC 3601560 2.9083898 36.262115 75-79
GCAGC 2847945 2.8858995 17.418755 20-24
AACCA 5850480 2.8366702 20.830023 70-74
GAGCC 2764110 2.8009472 63.162716 95-97
ATAGC 4704470 2.7998426 26.048872 80-84
TGCAT 4077845 2.7959676 29.241817 75-79
TTCAC 4328295 2.7854226 24.50022 45-49
ACCAT 4972935 2.7778485 30.730947 75-79
CACTG 3346775 2.7026415 34.45964 85-89
TGAAG 4257310 2.6995122 23.078909 60-64
AAGTT 5318860 2.687701 23.572487 60-64
CTCAG 3323265 2.6836562 36.35734 35-39
TTTCA 4906265 2.680802 23.985836 40-44
TTGAA 5197395 2.6263227 21.832666 55-59
GAGAG 3288660 2.6167166 43.927967 95-97
GCACT 3228755 2.607336 27.49879 85-89
AGCAC 3710240 2.6006713 28.702848 80-84
GGATT 3503440 2.5593119 26.284641 50-54
GACTC 3161880 2.553332 36.252056 25-29
GGAAC 3389820 2.531554 33.593643 70-74
TTGTA 4300200 2.5033958 27.72442 9
GCTGC 2094145 2.4447522 34.292053 1
GTAGT 3313175 2.4203205 26.230736 40-44
ATGCA 4029210 2.3979647 27.037624 75-79
GAAGA 4347820 2.3930023 8.305096 50-54
GTAGG 2594560 2.3783727 24.61454 7
CATTC 3685735 2.3719108 29.375458 30-34
TTAGC 3453600 2.3679552 31.311253 20-24
AAACT 5732360 2.3598816 32.785015 6
AGTTT 4041870 2.353007 26.731546 40-44
AGAAG 4251390 2.3399282 14.774321 6
TCAGT 3397590 2.329552 31.139505 35-39
CTGAA 3904395 2.3236818 25.485 85-89
GTTAC 3388120 2.3230586 28.861807 60-64
TCTCA 3586325 2.307937 23.384687 35-39
AAGAG 4184860 2.3033106 9.086961 50-54
CAGTA 3865815 2.3007212 26.885992 35-39
AGGAG 2881985 2.293134 17.51782 90-94
AAGGA 4139865 2.2785456 19.958012 70-74
CAGAC 3249855 2.2779672 31.442575 25-29
AGGAA 4134965 2.2758486 24.931711 70-74
GCATA 3816110 2.2711394 21.35329 80-84
GTTAG 3104670 2.2680047 33.12283 20-24
CTCAT 3522960 2.2671587 23.306618 30-34
GGAAG 2847155 2.2654204 10.628778 6
CATAG 3708920 2.2073457 26.528315 80-84
GTTGT 2600425 2.1885242 13.204173 35-39
TCATT 4002050 2.1867356 24.854628 30-34
TGTAA 4315440 2.1806574 23.245592 10-14
GATCG 2507280 2.1572075 7.5373964 95-97
TAACC 3850620 2.150931 25.106133 10-14
ACAGG 2868225 2.1420214 12.675053 60-64
TAGAG 3368000 2.1356108 26.64636 90-94
TAAGG 3354755 2.1272123 26.71966 65-69
AACCT 3796950 2.1209512 23.935038 10-14
CGTTA 3078755 2.1109433 24.539717 20-24
ACCAG 3002200 2.1043744 11.773499 55-59
TGTTG 2487375 2.0933812 12.107199 35-39
GCGTC 1790210 2.0899317 8.189116 95-97
ATTCT 3823500 2.0891752 25.059502 30-34
CTAAG 3497010 2.0812285 26.62104 65-69
GTAAC 3463175 2.061092 26.97033 10-14
GATGA 3248295 2.0597072 9.331508 15-19
GAGCG 1885885 2.0360663 9.267322 90-94
TAGTT 3496705 2.0356348 26.200878 40-44
CTGCA 2517340 2.0328426 16.26018 3
CAGCA 2882545 2.0205033 15.149882 20-24
TGAAT 3980360 2.0113366 20.660383 85-89
CGTCG 1719600 2.0075 8.519278 95-97
TAGGG 2181990 2.0001793 24.541977 8
TTTCC 2688020 1.9928991 11.330333 40-44
AGTTA 3935995 1.9889183 22.613445 60-64
ATCGG 2306730 1.9846588 6.908701 95-97
ACTGA 3317305 1.9742782 25.681707 85-89
AGGGA 2478080 1.9717556 21.475561 9
AGACT 3292530 1.9595333 26.940462 25-29
GGCTC 1670905 1.9506524 17.707281 65-69
TCGGA 2247885 1.9340297 6.6474276 95-97
ACTCA 3455290 1.9301021 23.847622 25-29
TTACT 3511300 1.9185879 19.599312 65-69
CCAGA 2729010 1.9128838 15.788136 55-59
GAATA 4357470 1.9112535 15.245016 90-94
GGAGG 1647505 1.8950934 14.766354 90-94
TTAAA 5403565 1.8887593 16.222563 50-54
CCATC 2472570 1.8740598 12.391978 75-79
AGATC 3134760 1.8656372 6.3175273 95-97
CAGAA 3603040 1.8612877 11.058188 5
CAGGC 1836140 1.8606102 13.724458 65-69
AATAG 4235570 1.8577863 19.162445 90-94
TTCCG 1962190 1.8254986 13.930506 40-44
TGACC 2241400 1.8100114 66.40869 1
GTCGT 1825135 1.8090999 7.3835773 95-97
GGAGC 1669360 1.8022985 15.704563 95-97
TGCAG 2088920 1.7972598 17.556116 4
TTGTT 2678835 1.7966576 11.0745535 35-39
CGGAA 2403020 1.7946013 8.972554 5
ATTAA 5107175 1.7851592 16.21212 50-54
AGCGT 2042410 1.7572438 7.0378823 90-94
CCAAA 3598430 1.7447387 30.117893 4
TACTA 3640310 1.726528 21.311796 65-69
GATTA 3410030 1.7231401 22.60154 50-54
GATGT 2358100 1.722625 15.076504 30-34
TAAAC 4174190 1.7184187 17.758232 50-54
GGATG 1861330 1.7062378 16.645144 10-14
ATGAT 3347575 1.6915807 8.263375 15-19
CCTCC 1643350 1.6900669 8.967188 1
TGATG 2308915 1.6866946 11.619895 30-34
TGTTT 2491970 1.6713299 14.228201 35-39
GAGAT 2630345 1.6678721 13.215239 25-29
TCCTC 1908640 1.6666228 7.382455 1
AGCTG 1933965 1.6639401 7.951019 9
ACCAA 3417235 1.6568842 19.304592 3
ATAGA 3719195 1.6312962 19.036451 90-94
CAGAG 2173495 1.6231896 11.577757 25-29
TCCGT 1711575 1.592342 14.315278 40-44
CCACC 1761730 1.5726595 6.828846 1
ACTAA 3782355 1.5571092 18.712194 65-69
TCGTG 1559145 1.5454466 6.3032956 95-97
TGTAG 2114625 1.544763 5.093503 95-97
AAGAA 4033185 1.5355133 8.753047 45-49
AGGCT 1779330 1.5308957 12.838424 65-69
GAGCT 1774560 1.5267917 8.668605 95-97
CAGGA 2033010 1.5182738 9.64019 90-94
CATCA 2692070 1.5037725 7.3164907 80-84
GGGGG 902170 1.500232 5.787855 1
GCTCA 1856390 1.499102 16.358854 65-69
CTACA 2644835 1.4773873 9.126309 85-89
TGCTG 1464050 1.4511871 5.744659 1
GGGAT 1575540 1.4442608 18.65383 10-14
AAAGA 3765760 1.4336994 6.5704083 45-49
CTCAC 1884935 1.4286675 12.397157 65-69
TACCA 2550345 1.4246058 8.465236 75-79
ATGTT 2443860 1.4227127 9.86754 30-34
GTGTA 1923785 1.4053518 5.0894876 95-97
CTGCT 1507995 1.4029438 6.083484 2
TAAAG 3176840 1.393411 7.6494274 45-49
GAGGA 1748975 1.3916223 13.393487 95-97
TACAG 2337935 1.3914107 8.106955 90-94
CGTGT 1398350 1.3860646 5.7266126 95-97
AACAG 2682655 1.3858278 12.241608 60-64
ATACC 2477710 1.3840325 8.198966 75-79
GACCA 1970720 1.3813648 25.63343 2
CTCTT 1836805 1.3618077 5.3334823 3
GAAAC 2620550 1.3537451 7.968376 60-64
GTAAA 3080170 1.35101 6.9143114 45-49
AGCCA 1925340 1.349556 10.922704 95-97
GAGAA 2412650 1.3279016 11.632611 50-54
GCTCC 1208495 1.3241763 6.0670857 1
CAAAC 2701010 1.3096145 32.129894 5
AGAAA 3421300 1.3025566 5.7612557 60-64
TCTAC 1999805 1.2869506 11.261485 85-89
CCGTA 1581540 1.2771506 16.312588 40-44
AGATG 2004910 1.2712908 10.642154 25-29
AAGTA 2846280 1.2484223 20.040863 8
GCCAA 1712975 1.2006999 12.434438 3
GATAC 2006185 1.1939713 7.5114455 75-79
AGAAC 2303555 1.1899892 9.702602 50-54
GGCCA 1161360 1.1768376 15.33816 2
AAACA 3271660 1.169086 6.007723 60-64
GCCCA 1229140 1.1690251 6.4406214 2
CAATA 2832800 1.1661991 7.564611 80-84
CCTCT 1335295 1.1659786 6.365039 2
CCGAA 1657550 1.1618501 34.59788 4
CTCCC 1129435 1.1615424 5.271471 1
CAACA 2395465 1.1614678 5.286244 8
GCTGG 932950 1.1604158 5.9568076 1
CCCAA 1736090 1.1421642 11.256902 4
TCAAT 2380790 1.1291622 6.513886 80-84
TGATA 2222735 1.1231819 8.156337 15-19
GTGCT 1123445 1.1135747 7.7099414 9
GATAG 1742195 1.1047062 12.965108 15-19
TTGGT 1307600 1.1004795 5.968901 7
CCTTT 1483560 1.0999119 5.0251713 4
ATCAA 2662405 1.0960513 5.854029 80-84
TGGCC 938270 1.0953575 30.29673 1
CCAAC 1662910 1.0940195 9.083227 5
CGTAA 1811770 1.0782663 9.800968 40-44
TATCT 1878180 1.0262448 7.1702423 85-89
GGATA 1602605 1.0161937 9.942575 70-74
ATCTA 2124340 1.0075331 6.5212064 85-89
GTTGC 1015840 1.0069151 5.0913 1
TCAAC 1800860 1.0059485 6.1698294 7
CAACT 1780555 0.99460626 7.8027797 6
CCATA 1759730 0.9829736 5.291498 3
GGTGC 774965 0.96391183 9.590209 2
AATAT 2747635 0.9604069 5.2243466 80-84
TCCCA 1265530 0.95919573 5.59657 3
GGGTG 712990 0.94485646 10.935887 1
CCGCC 731065 0.94344693 6.2724576 1
CCCTC 915825 0.9418599 5.376222 1
ACCCA 1420370 0.9344537 8.056412 3
GTGCA 1080495 0.9296336 7.1529236 3
ACCGA 1324920 0.928695 23.842503 3
CTCCG 846355 0.927371 6.5277433 2
GAACT 1556670 0.9264446 30.002773 6
CCCAT 1207415 0.915148 6.110693 4
TGGTC 917890 0.9098257 6.4130416 8
CTGCC 828115 0.90738493 5.82092 2
CCTAA 1623555 0.9069071 13.17216 4
GACCT 1117670 0.90255886 13.700593 2
ATTGG 1233280 0.90092826 6.233013 6
CGAAC 1277930 0.8957577 32.36765 5
CCTGG 765760 0.89396554 5.117592 1
TCCCC 860990 0.8854661 5.6051784 1
TGTGC 888205 0.8804015 7.2450743 8
ACCTA 1565480 0.8744667 9.719818 3
GCTGT 880600 0.87286335 7.430703 1
TGTCC 935040 0.8699025 6.750068 1
GGCTG 698755 0.8691209 6.8032126 1
ATATC 1818835 0.86263794 5.694817 85-89
CCGTT 911515 0.8480164 6.6739693 4
CGCCG 613470 0.84349394 5.0089192 2
TGCCC 760190 0.8329579 7.4828796 1
CTGTC 878950 0.8177199 7.6580763 4
GCAGG 751040 0.81084865 6.1775107 1
CGCCC 614515 0.79303795 6.400148 1
GGCCT 676445 0.7896973 7.4390087 2
CCCCC 637845 0.77259076 7.971635 1
CGTCC 704020 0.7714112 7.9314284 1
TGTCA 1123165 0.7700961 6.0027957 5
CCGTC 698385 0.7652368 6.46752 4
GGTGT 713200 0.7531926 5.813298 6
GTCAA 1221040 0.7266961 6.2473555 6
GTGTG 677065 0.7150313 7.7634664 7
GACCG 701575 0.71092486 33.203514 2
CGACC 735060 0.6991096 8.762017 1
GGGCC 465875 0.6824725 7.965015 1
CCCCG 524920 0.67741466 7.650625 2
TGCGG 528215 0.6570009 13.651455 3
AGTCT 952275 0.6529258 5.0689635 7
CGGCC 474410 0.65229267 5.1357155 1
CAGGG 599390 0.6471221 6.183687 2
GGCCG 431880 0.63267237 17.301758 2
GTCCC 576580 0.63177216 6.4241104 2
CTAAC 1130815 0.63166577 12.1028385 5
TAACT 1309905 0.6212624 10.840815 6
GGTAG 676645 0.6202647 5.5117993 6
GGTCA 715910 0.61595285 5.37717 9
GCCCC 476905 0.6154508 6.373233 1
GACCC 647085 0.61543727 10.968246 2
TGACT 889800 0.6100899 11.112074 1
GACTG 694645 0.59765697 7.821007 2
GCCGA 588895 0.5967432 12.262978 3
GCCGT 505235 0.58982277 5.403453 3
TCCCG 538240 0.58976215 5.742267 2
CCCGA 620015 0.5896912 6.813821 3
GACTT 854530 0.5859071 7.9707184 6
GGCCC 422950 0.5815374 6.4208117 1
GCCCG 387795 0.53320086 7.629082 2
GCGGA 493665 0.53297776 11.012424 4
CCCGT 471200 0.51630485 7.4503446 3
GTCTA 747670 0.5126386 5.1070275 8
GTCCG 398480 0.46519452 5.6435213 2
Produced by FastQC (version 0.10.1)