FastQCFastQC Report
Wed 6 Aug 2014
1L22C_ATCACG_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L22C_ATCACG_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 15250115
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 169807 1.1134801278547735 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 140403 0.9206684670902483 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 121515 0.7968136633723746 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 114471 0.7506238477545907 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 90682 0.5946315814667628 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 90148 0.5911299685281062 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 90014 0.5902512866296418 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 74595 0.4891438523578347 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 71485 0.4687505635203407 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 61829 0.40543300821010203 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 60236 0.39498718534253674 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 52337 0.3431908546263422 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 50029 0.3280565425244334 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 48822 0.3201418481106536 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 47778 0.3132959980957521 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 37863 0.248280094936989 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 37490 0.2458342117420098 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 37091 0.24321783802941813 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 36697 0.24063425095482888 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 36420 0.2388178712094958 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 36090 0.23665395310133724 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 33899 0.22228684832868473 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 33720 0.22111308668819876 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 33086 0.21695574098949416 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 32006 0.20987382718097536 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 30134 0.19759850991287609 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 29145 0.19111331291600098 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 28512 0.1869625245448969 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 26489 0.17369705080912506 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 26411 0.17318557925628758 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 26275 0.17229378270262224 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 24748 0.1622807434566887 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 24402 0.16001190810692248 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 24038 0.15762504086034762 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 23463 0.1538545774900714 No Hit
GCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGA 22906 0.1502021460166038 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 22316 0.14633332273232036 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 22086 0.1448251373842099 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 21109 0.13841862831854054 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 20667 0.13552028951912823 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 20605 0.13511373520789843 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 19829 0.13002524898992565 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 19742 0.12945476148868384 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 19323 0.12670724122408256 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 19301 0.126562980016872 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 18752 0.12296300716420827 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 18744 0.12291054854340441 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 18562 0.121717114920117 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 18251 0.1196777860363676 No Hit
TGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCT 17322 0.11358602869552131 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 17307 0.11348766878151412 No Hit
CTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCC 16843 0.1104450687748912 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 16713 0.10959261618682875 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 16406 0.10757951661348128 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 16123 0.10572379290254533 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 15731 0.10315332048315702 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 15505 0.10167136444544844 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 15494 0.10159923384184316 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 15341 0.10059596271896964 No Hit
CCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTG 15314 0.10041891487375669 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGCT 4774800 3.5560203 8.940883 5
ACTTC 5056530 3.4617925 6.0801845 55-59
AAAAA 5319785 3.2402985 5.493256 45-49
GGAAG 4020290 3.1523178 6.541628 85-89
AACTT 5663020 3.0994754 5.776347 55-59
AGCAC 2916605 3.0237494 7.363247 90-94
GAAGA 4119945 2.9696374 6.402415 90-94
CTACT 4261915 2.9177847 9.687168 2
AGCTG 3800370 2.9041343 9.398192 3
CTTCA 4065510 2.783322 6.165987 60-64
TCTCT 4706240 2.7308192 8.147212 20-24
CTGGT 4183075 2.7092986 13.655561 1
CGGAA 2992275 2.6978755 7.2673573 95-97
TTCAA 4878785 2.670249 7.710058 9
CTTCT 4346045 2.5218143 5.6446877 40-44
CAACT 3114190 2.5154932 9.597502 9
CGGTG 2994465 2.4892619 14.430872 9
GCTGC 2592995 2.478568 6.8885264 3
GGTGT 4379965 2.467085 12.074565 1
GGTGG 3408750 2.4643292 11.950372 7
TGGTG 4371890 2.4625363 10.389719 6
CCGTG 2558085 2.4451983 6.880156 60-64
CTGAA 3445565 2.42042 5.1345916 80-84
ACATC 2984880 2.4110427 5.6922193 40-44
AGAGC 2663265 2.4012356 7.232528 95-97
TATGC 4028820 2.398714 6.7446733 30-34
TCTGC 3216175 2.3952382 6.2202344 85-89
ATGCA 3373685 2.369926 7.003885 1
ACAAC 2473010 2.3568585 5.2700286 40-44
CATGG 3063895 2.341341 9.262421 2
ATCGG 3027530 2.313552 6.280071 95-97
CAACA 2420160 2.3064907 5.158137 85-89
TCGGA 3014715 2.303759 6.0587335 95-97
AAGAG 3177115 2.29005 5.753497 90-94
ACCCA 1916815 2.2850528 24.523865 1
CGTGA 2967380 2.267587 7.375256 9
GATCG 2965585 2.2662153 6.8389716 95-97
GAGCA 2512720 2.265502 6.9269447 90-94
GCAGC 2007880 2.2644699 5.6085477 1
TCAAC 2792475 2.2556272 8.654575 8
CAAGC 2144995 2.2237935 5.7658887 2
ATGGT 4269155 2.2105217 5.674352 1
GCACA 2124670 2.2027218 7.731298 95-97
GAAGC 2432405 2.193089 5.100235 15-19
GTTCC 2940910 2.1902354 5.0446944 8
CAGCA 2111410 2.1889749 5.7548313 2
TCCTT 3771505 2.1884346 6.799003 40-44
GCTGG 2597410 2.159195 10.804319 4
ACTGC 2440570 2.1445186 9.036799 4
CACAC 1756770 2.0942616 8.954903 95-97
AGATC 2975525 2.0902288 6.321098 95-97
GGCTC 2174255 2.0783067 6.548954 10-14
CCAAG 2003690 2.0772977 6.0305653 25-29
ATCCG 2354480 2.0688717 5.3304663 65-69
CTCTC 2412950 2.066356 5.921221 15-19
CCTTA 2965585 2.0302935 7.225128 45-49
TGGCT 3052335 1.9769397 9.496553 9
TATTC 4248865 1.9709854 7.3900986 2
CTCTG 2640230 1.9663045 6.1734786 45-49
CTCTA 2844285 1.9472493 5.6169033 25-29
GTGGC 2327060 1.9344565 11.358098 8
GCATG 2528215 1.9319898 6.4072185 3
CTTGG 2947990 1.9093574 5.656586 2
GCAGG 1907420 1.8707998 6.5093555 90-94
TTCGG 2885565 1.8689259 11.093738 7
TGCTG 2885125 1.868641 7.0066967 6
GCTAC 2115110 1.8585386 11.802003 1
TAGCT 3109560 1.8513972 6.829271 2
CATCC 1823360 1.8422935 5.2584934 40-44
CACCC 1229740 1.8337395 7.345426 50-54
CTCCT 2138575 1.8313919 5.8403835 70-74
TTTCA 3924700 1.82061 6.6289487 8
GCTCT 2444290 1.8203788 6.2871094 10-14
TACTG 3047560 1.814483 6.360425 3
AATGA 3219860 1.8082517 5.852098 75-79
TCGGT 2766220 1.7916285 12.081822 8
GTCTG 2741045 1.775323 7.176166 90-94
CCAGC 1368680 1.7749187 6.644608 1
GCACC 1355865 1.7583001 6.6433287 50-54
CACAA 1829550 1.7436203 5.324244 35-39
CAGTG 2274925 1.7384328 5.660903 30-34
GCTTC 2302715 1.7149411 6.2390213 15-19
CGCAG 1520290 1.7145699 7.5617943 90-94
CCTGT 2283175 1.7003888 7.8867874 1
ACTAC 2072305 1.6739084 5.286569 70-74
CTGAG 2180750 1.666467 5.52723 80-84
CTGTT 3298655 1.6645927 5.637222 8
GTGTA 3201280 1.6575878 10.831098 2
CTTTC 2837625 1.646546 7.911857 7
TTGGT 3740920 1.6417298 10.934031 7
AATCC 2030985 1.6405321 5.2228374 60-64
AGTGC 2115130 1.6163218 5.031463 25-29
TGTAG 3119880 1.6154397 6.707159 3
CCACA 1353085 1.6130248 6.189797 60-64
CCCAA 1332175 1.5880979 24.505371 2
ACACG 1530025 1.5862321 7.642989 95-97
GTAGC 2068025 1.5803256 8.0921135 1
TGGTA 2972660 1.5392108 9.527038 2
CCAAT 1891090 1.5275316 17.065468 3
GCTTG 2354485 1.5249555 5.2951803 1
TACTT 3193360 1.4813524 6.99455 5
ACACA 1536920 1.4647344 5.2243867 35-39
ATGCC 1636655 1.4381219 6.140307 9
GTATT 3553920 1.4337399 6.799476 4
CCGCC 881395 1.4297347 6.163369 45-49
GCTGT 2200370 1.4251381 7.2001133 7
AACGC 1372250 1.422661 6.330589 95-97
CAGGT 1835870 1.4029194 5.6513844 95-97
ACATG 1977975 1.389476 8.139981 1
CACGT 1578730 1.3872237 5.6356845 95-97
ACGGT 1775480 1.3567711 5.9260097 4
AGCCT 1536085 1.3497517 5.777678 2
GGTCA 1759715 1.3447239 15.182826 9
GGTAC 1748655 1.3362722 11.128171 3
ACTTT 2839265 1.317093 6.833357 6
GCGGC 1063330 1.3045399 5.433523 7
GCCTG 1341120 1.2819372 5.7875476 3
GACGG 1299355 1.2744089 7.5769305 3
TGTAT 3155675 1.273078 6.609776 3
ATTCG 2120930 1.2627779 9.054506 6
ACGTC 1434480 1.2604718 5.328911 95-97
TGGTC 1945625 1.2601446 13.066768 8
ATGGG 1827045 1.2142055 7.9239273 3
CAATT 2188855 1.1980008 11.586608 4
CTAAC 1480260 1.1956829 5.3522253 95-97
AAGCC 1105145 1.1457436 5.8332376 1
GTACT 1907570 1.1357458 8.545892 4
GGTCG 1349225 1.1215942 7.1843867 6
CTGAC 1272495 1.1181362 6.7208157 1
TGGGT 1975460 1.1127093 5.776976 4
TGACG 1438535 1.0992874 5.8544927 2
ATTGG 2077490 1.0757016 10.720576 6
TGGCC 1098660 1.050177 8.6355 1
GGGTC 1223025 1.0166856 7.180715 5
TCGTG 1502875 0.9733837 5.6117053 8
TGGGG 1342085 0.9702499 5.141264 8
AATTG 1947140 0.9268074 9.168506 5
GGGGT 1237310 0.89450353 5.46579 8
CGGTA 1152480 0.8806923 5.4314656 5
GGCCT 786900 0.7521747 5.56546 2
GCCTA 826985 0.7266683 5.5047164 3
GCCCT 624560 0.68646926 5.501155 1
GTCGT 1019730 0.6604599 5.3504767 7
CCCCG 401800 0.6517708 5.5543485 1
Produced by FastQC (version 0.10.1)