FastQCFastQC Report
Thu 31 Jul 2014
1L22B_ATCACG_L003_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L22B_ATCACG_L003_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9662537
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 102622 1.0620606161715085 No Hit
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 99883 1.0337140235530276 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 70023 0.7246854526921863 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 59643 0.6172602495597171 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 57419 0.5942435201024328 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 48135 0.49816109371689854 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 42321 0.43799056086408783 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 42113 0.4358379171018957 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 41695 0.4315119310797982 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 41396 0.4284175056716471 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 36791 0.3807592146865777 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 34206 0.35400640639202724 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 32771 0.3391552342826734 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 28905 0.2991450382026998 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 25723 0.2662137283407039 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 25702 0.2659963941147133 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 25186 0.26065618170465993 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 20589 0.21308068471044406 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 20021 0.20720231135984268 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 19326 0.20000958340444128 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 19253 0.19925408823790272 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 18948 0.1960975673366115 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 18227 0.18863575891093612 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 18053 0.1868349896098716 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 17855 0.18478583833624648 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 17687 0.18304716452832212 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 17596 0.18210538288236308 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17227 0.17828651005424354 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 17073 0.17669272573031286 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15556 0.16099291521471018 No Hit
CGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15031 0.15555955956494658 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 14105 0.1459761551236492 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 13410 0.13878342716824785 No Hit
GCTGCTGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 13396 0.13863853768425416 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 13176 0.1363617029357818 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 13166 0.13625821044721484 No Hit
TGCCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 13037 0.1349231573447015 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 13026 0.13480931560727788 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 12382 0.12814439934356786 No Hit
TGGCTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 12004 0.12423238327573805 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 11941 0.12358038059776641 No Hit
TGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 11677 0.12084817889959956 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 11620 0.12025827171476809 No Hit
CGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 11596 0.12000988974220746 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 11459 0.11859204264884056 No Hit
GCTGCCGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 11286 0.11680162259663275 No Hit
AGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 11143 0.1153216800101257 No Hit
GACCACACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 11119 0.1150732980375651 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 10326 0.10686634369420785 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 6526580 5.709255 9.86317 7
AGCAG 3988160 4.6461725 50.664337 20-24
TAGCA 4724485 4.4475546 34.45248 20-24
ACTTG 3980170 4.3230357 97.801636 8
CACGG 2693875 4.191586 42.62357 45-49
CCTGC 2324860 3.9040923 46.94951 15-19
GTTTC 3065505 3.8415775 38.037388 40-44
GCAGA 3255440 3.7925599 33.51701 25-29
CTGCG 2016795 3.6206217 49.50657 15-19
GAAGT 3515140 3.5375905 27.692415 60-64
TCACG 2794190 3.5131845 34.402557 45-49
CTTGA 3146230 3.4172573 30.53823 55-59
AGAGC 2930130 3.4135768 51.421078 95-97
CGGAT 2510570 3.3745468 33.76114 45-49
GAACC 3000010 3.2692444 30.02259 70-74
AACTT 4218085 3.2086785 68.62272 7
AGAGA 3656825 3.1896918 21.87119 90-94
AGTAG 3126545 3.1465132 25.87027 9
CTTGT 2425475 3.0395155 112.91012 9
TTCTC 2582300 3.0270264 30.513573 30-34
GCGTT 1950940 3.0255718 39.3421 15-19
ACGGA 2583090 3.0092776 31.48355 45-49
CCATG 2385145 2.9988852 34.811817 75-79
TGCGT 1899360 2.9455798 42.380352 15-19
ACCTG 2271795 2.856368 26.688889 15-19
CATGC 2268020 2.8516214 34.36908 75-79
GCAGC 1801910 2.8037164 16.473698 20-24
TGCAT 2572440 2.794039 27.7759 75-79
TTCAC 2738520 2.7823064 22.388393 45-49
AAGTT 3418130 2.7796974 23.13596 60-64
AACCA 3635195 2.7744443 19.678125 70-74
TTGAA 3405540 2.769459 21.025625 55-59
TGAAG 2744325 2.7618523 21.01454 60-64
ATAGC 2931890 2.7600343 25.304323 80-84
GAGCC 1753795 2.728851 59.5861 95-97
ACCAT 3073415 2.7063866 27.563097 75-79
CACTG 2120255 2.6658342 32.93311 85-89
TTTCA 3024405 2.6544294 23.092903 40-44
CTCAG 2082435 2.6182826 34.408047 35-39
GAGAG 2087280 2.5995703 40.038925 95-97
AGCAC 2377675 2.5910583 27.016031 80-84
GCACT 2046725 2.5733836 24.780704 85-89
GGATT 2163975 2.512682 23.875046 50-54
GACTC 1969420 2.4761868 34.316853 25-29
GGAAC 2120600 2.4704807 31.843079 70-74
TTGTA 2627560 2.4653692 32.832047 9
ATGCA 2570495 2.419823 26.166904 75-79
GAAGA 2766500 2.4130993 8.17781 50-54
AAGAG 2741070 2.3909178 9.122714 50-54
GTAGT 2055860 2.3871453 23.876024 40-44
GTAGG 1651305 2.3728404 18.621017 7
AGAAG 2704260 2.35881 14.387729 6
AGTTT 2505075 2.350445 26.166346 40-44
TTAGC 2158640 2.3445928 30.169428 20-24
AAACT 3549700 2.3403568 30.50102 6
CATTC 2295780 2.3324876 28.221874 30-34
GCTGC 1290910 2.3174872 30.224987 1
CTGAA 2450945 2.3072803 24.803661 85-89
GTTAC 2124200 2.307186 27.289015 60-64
GGAAG 1851660 2.3061209 9.419871 6
CAGTA 2441035 2.297951 25.833519 35-39
GCATA 2427690 2.2853885 19.374306 80-84
TCTCA 2244880 2.2807736 21.193565 35-39
AAGGA 2610120 2.2766957 18.101566 70-74
TCAGT 2094925 2.2753892 29.990446 35-39
GTTAG 1932335 2.243715 31.857227 20-24
CTCAT 2190040 2.2250571 21.093668 30-34
CATAG 2358875 2.220607 25.530293 80-84
CAGAC 2026400 2.2082577 29.703836 25-29
AGGAG 1771320 2.2060628 15.384029 90-94
TCATT 2512985 2.2055714 24.244518 30-34
AGGAA 2524280 2.201821 23.955988 70-74
GTTGT 1605920 2.1514416 12.76483 35-39
GCGTC 1195970 2.1470478 7.046854 95-97
GATCG 1597295 2.1469815 6.563507 95-97
TGTAA 2631115 2.1396797 22.7433 10-14
TAGAG 2124415 2.137983 26.065718 90-94
GAGCG 1278025 2.1258783 8.694382 90-94
TAAGG 2097050 2.1104434 25.31816 65-69
ACCAG 1935425 2.1091182 11.039989 55-59
CGTCG 1171915 2.1038632 7.450941 95-97
AACCT 2373275 2.0898576 22.663092 10-14
TAACC 2368025 2.085235 24.154634 10-14
CGTTA 1912940 2.0777273 22.229366 20-24
CTAAG 2200325 2.0713508 25.525501 65-69
ATTCT 2344710 2.0578816 24.430155 30-34
TGAAT 2518155 2.0478182 20.215881 85-89
ACAGG 1746680 2.0348673 11.345319 60-64
TAGGG 1409450 2.0253072 18.514132 8
AGGGA 1625760 2.0247774 16.110016 9
TGTTG 1509970 2.022898 11.554811 35-39
TAGTT 2154865 2.0218523 25.612759 40-44
CTGCA 1601970 2.0141852 15.746192 3
GTAAC 2123860 1.9993678 25.995657 10-14
ATCGG 1484975 1.9960079 6.0288277 95-97
CAGCA 1825685 1.9895297 13.597455 20-24
AGATC 2097810 1.9748449 5.9292545 95-97
GAATA 2792040 1.9679328 14.257251 90-94
GATGA 1954985 1.9674711 9.250143 15-19
AGTTA 2416160 1.9648737 22.09903 60-64
TCGGA 1459935 1.9623507 5.8121305 95-97
ACTGA 2077680 1.9558946 24.950333 85-89
AGACT 2053985 1.9335887 25.958546 25-29
CCAGA 1769245 1.9280246 14.4202175 55-59
TTTCC 1644255 1.9274304 10.883764 40-44
TTACT 2161865 1.897404 17.995152 65-69
TTAAA 3326930 1.8948562 15.970124 50-54
AATAG 2684885 1.8924056 18.898857 90-94
ACTCA 2143735 1.8877296 22.490543 25-29
GTCGT 1213750 1.882317 6.6416826 95-97
CAGAA 2276740 1.8576325 11.002045 6
AGCGT 1373090 1.8456193 6.7553744 90-94
TGCAG 1370300 1.8418694 17.052876 4
CGGAA 1569635 1.8286114 8.3977995 5
ATTAA 3173435 1.807433 16.02664 50-54
GGCTC 1004365 1.8030716 16.688175 65-69
CCATC 1520980 1.7888356 10.650678 75-79
GGAGG 1003975 1.7853365 12.915866 90-94
CAGGC 1137755 1.7703115 12.749062 65-69
TTGTT 1615950 1.7493538 10.798291 35-39
TACTA 2283235 1.7368467 20.674782 65-69
TAAAC 2633915 1.7365698 17.045687 50-54
CCAAA 2272365 1.7343086 25.097338 4
GGAGC 1035000 1.7216283 14.183109 95-97
TGTAG 1481200 1.7198834 5.1038427 95-97
GATTA 2100275 1.7079891 22.026651 50-54
TGATG 1457980 1.6929216 11.305318 30-34
TCCTC 1242595 1.6861528 8.952658 1
GATGT 1444985 1.6778326 13.754727 30-34
CCTCC 1067560 1.6769398 8.493427 1
TCGTG 1074375 1.6661705 5.972625 95-97
GAGAT 1625515 1.6358967 12.072309 25-29
ACCAA 2137995 1.6317551 13.763301 3
TTCCG 1120035 1.6247892 13.265708 40-44
ATAGA 2301470 1.6221606 18.796553 90-94
GGATG 1123015 1.6137148 15.007368 10-14
ATGAT 1981340 1.6112686 8.242526 15-19
GTGTA 1381495 1.6041116 5.1053443 95-97
TGTTT 1473675 1.5953332 13.114324 35-39
CCACC 1170075 1.59301 6.960504 1
CAGAG 1365330 1.5905976 11.0810375 25-29
AGCTG 1177755 1.5830628 6.279819 1
TGACC 1257250 1.5807627 42.246082 1
TCTCC 1151845 1.5630085 5.445723 1
GAGCT 1161065 1.5606289 7.3879113 95-97
ACTAA 2337365 1.5410508 18.21147 65-69
AAGAA 2516790 1.5375 8.275398 45-49
CGTGT 970490 1.5050626 5.4184194 95-97
AGCCA 1362985 1.4853053 12.939889 95-97
CTACA 1678635 1.4781721 8.731189 85-89
TGCTG 953005 1.4779465 5.647416 1
CAGGA 1268125 1.4773546 9.264508 90-94
TCCGT 1014790 1.4721146 13.613187 40-44
CATCA 1666640 1.4676094 6.941776 80-84
AAAGA 2379970 1.453917 6.502247 45-49
AACAG 1768120 1.4426405 11.761327 60-64
AGGCT 1071455 1.4401809 12.297186 65-69
ATACC 1626890 1.4326065 8.072823 75-79
GCTCA 1131005 1.4220326 14.720566 65-69
TACAG 1503105 1.414999 8.297993 90-94
TACCA 1583295 1.3942175 8.295085 75-79
ATGTT 1457315 1.3673598 8.910815 30-34
CTCAC 1153710 1.3568867 10.858039 65-69
TCTTG 1079660 1.35299 5.2955194 4
TAAAG 1919535 1.3529589 7.486666 45-49
CTGCT 930960 1.3505058 5.4864945 2
GACCA 1235525 1.3464066 16.919464 2
GAAAC 1649800 1.3461013 7.6939497 60-64
GGGAT 933340 1.3411617 16.758694 10-14
GAGGA 1072725 1.3360085 12.66313 95-97
CAAAC 1747660 1.3338447 28.113827 5
GCTCC 791450 1.3290668 6.182805 1
GAGAA 1491290 1.3007883 10.57806 50-54
CTCTT 1109655 1.3007609 6.031987 3
AGAAA 2110345 1.289204 5.5938454 60-64
CAACA 1683555 1.2849185 7.1341743 8
GTAAA 1810680 1.2762338 6.593451 45-49
GCCAA 1149180 1.2523125 11.20089 3
AAGTA 1767315 1.2456685 19.255316 8
CCGTA 990510 1.2453858 14.56142 40-44
GGCCA 786885 1.2243689 12.52366 2
AGATG 1212265 1.2200074 9.47444 25-29
GCCCA 837580 1.2190715 7.3179255 2
TCTAC 1196055 1.2151788 10.171291 85-89
GATAC 1271015 1.1965132 7.192075 75-79
CCCAA 1146265 1.1684538 10.029786 4
CCTCT 858570 1.1650459 7.0188127 2
AGAAC 1416065 1.1553926 8.686302 50-54
AAACA 2009940 1.1485586 5.8708286 60-64
CAATA 1720060 1.1340548 6.9387293 80-84
GCTGG 590350 1.1329978 5.07916 1
CTCCC 720375 1.1315762 5.2858152 1
CCGAA 1033880 1.126665 30.112225 4
TGATA 1365600 1.1105355 8.00882 15-19
TCAAT 1443445 1.0980221 6.338208 80-84
TGCCA 866580 1.0895663 5.5289497 1
ATCAA 1646070 1.0852723 5.65409 80-84
CCAAC 1062335 1.0828992 7.3831105 5
TCAAC 1215080 1.0699749 8.309918 7
TGGCC 595240 1.0685959 24.94086 1
GATAG 1052610 1.0593327 11.810851 15-19
CCTTT 901005 1.0561769 5.079151 4
ACCGT 839210 1.0551535 5.2481203 3
CGTAA 1089650 1.025779 8.706634 40-44
CCATA 1163300 1.0243784 6.0302973 3
CCCTT 748605 1.0158277 5.1031632 3
TATCT 1125700 0.98799306 6.997019 85-89
GGTGC 507105 0.97323424 10.688558 2
GTGCA 722050 0.9705332 8.336855 3
CCGTT 665105 0.96484077 7.8239317 4
CAACT 1087815 0.95790774 6.483036 6
CGCCG 459460 0.9548319 5.444157 2
GGATA 947380 0.95343065 8.867219 70-74
ATCTA 1252315 0.9526304 6.2870436 85-89
CTCCG 560075 0.9405231 7.584564 2
CCCTC 595110 0.934808 5.4351835 1
CCTAA 1059675 0.93312836 10.733096 4
CCGCC 476245 0.92578804 6.868614 1
ACCTA 1045820 0.92092794 7.085471 3
TGTCC 633675 0.91924655 8.560902 1
CTGCC 544795 0.91486377 5.1568527 2
GAACT 963840 0.9073436 25.948433 6
GACCT 717170 0.90171057 9.859425 2
GGGTG 438610 0.8999038 11.716789 1
CTGTC 609270 0.88384324 9.48189 4
ACCGA 810215 0.8829272 18.075686 3
TGCCC 519520 0.87241995 9.384601 1
GGCTG 452435 0.86831176 6.5018845 1
TCCCC 551850 0.86685455 5.911486 1
ACCCA 847740 0.86415017 6.277452 3
TGTCA 790405 0.8584931 7.506975 5
CGAAC 783410 0.85371673 27.554108 5
GCTGT 548915 0.85127246 6.58188 1
AACAA 1456840 0.8324955 5.7101545 9
ATATC 1089085 0.82846206 5.4635262 85-89
CCGTC 488740 0.82073164 7.745428 4
GGCCT 440070 0.7900292 7.194683 2
CGTCC 457205 0.76777554 6.670814 1
CGCCC 393145 0.76424724 6.919542 1
GTCAA 811580 0.7640084 8.171267 6
TGGCT 487100 0.7554081 5.914513 1
CCCCC 413980 0.7527701 8.301381 1
CCCCT 474875 0.745941 5.0981073 2
CGACC 505460 0.7356812 8.40075 1
GGGCC 315965 0.7019664 7.8898025 1
GCCGT 390475 0.7009945 5.005194 3
AGTCT 644620 0.70014983 5.9983644 7
TACTT 766955 0.6731334 5.244128 6
CCCCG 342330 0.66546637 9.009042 2
CGGCC 314385 0.65334266 5.0118804 1
TGCGG 337545 0.6478152 12.164303 3
TGACT 592760 0.64382243 11.592252 1
GACTG 473045 0.6358367 8.40857 2
GGCCG 282395 0.6273853 15.199024 2
TCCGA 498420 0.62667227 5.820266 3
GCCCC 319755 0.6215821 7.568396 1
GTCCC 367265 0.61674106 5.5709605 1
GACCG 395700 0.615697 24.536287 2
TAACT 805595 0.6128125 9.078961 6
GCCGA 391705 0.6094809 11.855639 3
CTAAC 689445 0.60711133 9.6137085 5
TCCCG 359515 0.60372657 6.40248 2
CCCGA 408855 0.59507567 8.6573925 3
GACTT 544895 0.5918341 8.743988 6
GGCCC 281970 0.5859791 7.536473 1
GACCC 396710 0.57739896 8.334696 2
GCCCG 269405 0.55986696 9.410327 2
GTCTA 512455 0.5565997 6.010927 8
CCCGT 312075 0.5240615 8.097004 3
GCGGA 311045 0.5173951 9.755697 4
GTCCG 269035 0.48298115 6.789705 2
Produced by FastQC (version 0.10.1)