FastQCFastQC Report
Thu 31 Jul 2014
1L22B_ATCACG_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L22B_ATCACG_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9662537
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 104009 1.0764150243357413 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 85169 0.8814351758756526 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 74951 0.7756865510579675 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 67510 0.6986777903153177 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 63904 0.6613583989380843 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 60925 0.630527986593997 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 60804 0.6292757274823372 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 59030 0.6109161600105645 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 51383 0.5317754540034362 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 49378 0.5110252100457675 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 42227 0.43701773147155865 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 38331 0.3966970579258843 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 35689 0.3693543424465024 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 35457 0.36695331671174974 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 33393 0.3455924670715362 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 29992 0.31039467170992463 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 29641 0.3067620853612255 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 27750 0.2871916557732198 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 25913 0.2681800856234755 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 23447 0.24265883794287155 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 23190 0.23999908098670153 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 22960 0.23761875374966224 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 22674 0.23465886857664814 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 22417 0.23199911162047815 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 22191 0.2296601813788656 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 21971 0.22738334663039322 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 21707 0.2246511449322264 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 18721 0.19374828784614226 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 18672 0.19324117465216434 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 17072 0.17668237648145615 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 17031 0.17625805727833177 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 16347 0.16917917106035402 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 15615 0.16160352089725505 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 15596 0.1614068851689779 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 15492 0.16033056328788187 No Hit
TGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCT 14555 0.15063331710916086 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 14368 0.14869800757295937 No Hit
CTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCC 14062 0.1455311374228114 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 14047 0.14537589868996104 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 13643 0.14119480215185723 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 13620 0.1409567694281533 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 13459 0.1392905403622258 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 13454 0.13923879411794232 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 13446 0.13915600012708876 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 13173 0.1363306551892117 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 13153 0.13612367021207786 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 13124 0.13582354199523375 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 13029 0.13484036335384797 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 12969 0.13421940842244642 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 11711 0.1212000533607271 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 11666 0.12073433716217595 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 11559 0.11962696753450983 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 11409 0.11807458020600595 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 11334 0.117298386541754 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 11323 0.11718454480433038 No Hit
GCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGA 10993 0.11376929268162182 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 10842 0.11220655610426124 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 10693 0.11066451802461404 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 10585 0.10954679914809123 No Hit
CCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTG 10179 0.10534500411227403 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 10146 0.10500347890000318 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 10138 0.10492068490914963 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3498095 3.5791445 6.0601506 95-97
CTGCT 3083450 3.5747082 9.345396 5
ACTTC 3212490 3.5282495 7.289326 55-59
AACTT 3622625 3.2358224 6.926938 55-59
GGAAG 2624390 3.1581645 5.871578 85-89
AGCAC 1873520 3.0591764 6.691941 90-94
GAAGA 2673980 3.0484445 5.7622232 90-94
CTACT 2664275 2.9261496 9.889747 2
AGCTG 2462685 2.9088 11.159401 3
TTCAA 3222790 2.8786795 7.402593 55-59
TCAAG 2560880 2.8655505 5.3722267 60-64
CTTCA 2587600 2.8419383 6.2869816 60-64
TGCTA 3009690 2.8377182 5.529168 95-97
CAACT 2126845 2.7722 8.232565 9
CTGGT 2729290 2.7163355 12.463231 1
TCTCT 2885750 2.6705759 8.637352 20-24
CGGAA 1862375 2.6106186 6.2469163 95-97
TCAAC 1980555 2.581521 7.7789207 8
CTTCT 2706075 2.5042982 5.8894496 40-44
GCTGC 1720770 2.4991024 7.309469 3
TATGC 2636855 2.4861867 6.413811 35-39
ACATC 1903010 2.4804463 5.4788795 40-44
TGGTG 2847475 2.432897 10.675745 6
CTGAA 2173165 2.4317083 5.0340405 80-84
GGTGG 2244025 2.4018674 10.135303 7
AGAGC 1711220 2.398734 5.920953 90-94
GAAAC 1800180 2.390595 5.7721863 75-79
GGTGT 2795045 2.3881006 12.23153 1
CGGTG 1903700 2.3735027 13.529731 9
ACAAC 1523990 2.357448 5.2385616 40-44
AAGAG 2059205 2.3475764 5.177211 90-94
CATGG 1956785 2.3112566 10.986288 2
ATGCA 2062970 2.3084035 6.4996047 1
CCGTG 1584785 2.301609 6.509777 70-74
GAGCA 1636890 2.294541 6.2594724 90-94
ACTGC 1661995 2.2866764 9.422978 4
TCTGC 1972090 2.2862854 6.129078 90-94
ACCCA 1195530 2.2739277 24.311724 1
GCACA 1391140 2.2715225 6.7351604 95-97
GATCG 1912945 2.2594748 5.8431473 95-97
TCGGA 1893340 2.2363183 5.1835575 95-97
CAGCA 1365600 2.2298198 6.2638803 35-39
ATCGG 1885900 2.2275305 5.378475 95-97
GCAGC 1287685 2.2194333 6.1228943 1
CGTGA 1874520 2.2140892 9.494457 9
CACAC 1156535 2.1997583 8.021605 95-97
TCCTT 2353400 2.17792 6.8737783 40-44
GAGAA 1909180 2.176542 5.1691785 80-84
ATGGT 2654985 2.1490161 6.568587 1
GCTGG 1721615 2.1464822 12.586323 4
CAAGC 1306585 2.1334572 6.433027 2
GTTCC 1808450 2.096574 5.8140006 45-49
CAAGG 1487070 2.084528 5.4324603 4
AGATC 1857105 2.0780466 5.7505894 95-97
CAAGT 1834315 2.0525453 5.27063 60-64
CTCTC 1515430 2.0464895 6.280053 20-24
TATTC 2708215 2.0383236 7.011323 5
CCAAG 1240800 2.0260398 5.229345 25-29
TGGCT 2030395 2.020758 7.884862 9
CAGCT 1456710 2.0042324 5.7364793 65-69
GGCTC 1372675 1.9935583 6.536889 20-24
ATCCG 1436170 1.975972 5.578426 65-69
CCTTA 1793200 1.9694555 7.2048483 45-49
CTTGG 1963890 1.9545687 6.3980694 2
TAGCT 2065515 1.9474928 8.3256 2
CTCTG 1676550 1.9436595 6.03942 45-49
TTTCA 2580270 1.9420266 5.851528 8
CTCTA 1767925 1.9416964 6.888861 25-29
GCTAC 1386240 1.9072753 11.872938 1
CTCCT 1394145 1.8827021 7.2996235 70-74
GTGGC 1502505 1.8732992 9.220064 8
TGCTG 1881715 1.8727835 7.3257236 6
GCTCC 1106095 1.8712143 5.916674 70-74
TACTG 1965580 1.8532679 6.8034196 3
TTCCT 1995105 1.8463413 5.7247605 45-49
GCATG 1560080 1.8426882 5.7632 3
TCCGT 1565465 1.8148766 5.0115995 70-74
AATGA 1988510 1.8096327 5.544237 75-79
AAACT 1705035 1.8074481 5.0545855 75-79
TTCGG 1814520 1.8059076 10.660292 7
CTGTT 2258240 1.7940987 5.9375367 8
GTCTG 1793820 1.7853057 7.8386235 90-94
CACAA 1133695 1.7537038 5.41301 4
CCTGT 1510930 1.7516528 8.313224 1
GCAGG 1181235 1.7478284 5.5538216 90-94
GCTCT 1506425 1.74643 6.7547264 20-24
CACCC 742965 1.7375635 6.4123087 50-54
TCGGT 1732700 1.7244759 11.975882 8
CTATG 1826905 1.7225171 5.7746353 35-39
CTATT 2288070 1.7221041 5.5684032 25-29
CTTTC 1855825 1.7174467 6.869238 7
CAGTG 1438455 1.699031 6.7324204 30-34
ACACG 1038300 1.6953881 6.857355 95-97
GCACC 838685 1.6838417 5.732729 50-54
CCAGC 828610 1.663614 6.4120193 1
GTGTA 2047755 1.6575078 11.250175 2
TGTAG 2038520 1.6500329 7.479859 3
ACTAC 1263000 1.6462361 5.742367 75-79
GCTTC 1414305 1.6396334 6.0195312 15-19
CCAAT 1250490 1.6299299 17.043863 3
CTGAG 1378860 1.6286404 6.577931 80-84
AATCC 1245885 1.6239278 5.8040195 65-69
CCCAA 847905 1.6127362 24.578194 2
AAGTT 2089725 1.6024352 5.0891633 60-64
TTGGT 2348390 1.6016829 10.688103 7
GTAGC 1345975 1.5897982 9.864583 1
CCACA 834340 1.5869354 6.2819347 1
AGTGC 1340545 1.5833846 5.4099784 30-34
GCTTG 1577620 1.5701319 5.9884496 1
ATGCC 1138565 1.5665085 7.7790127 9
CGCAG 898630 1.5488642 6.5437555 90-94
TGGTA 1898005 1.5362961 8.845225 2
CTGTA 1608245 1.516351 6.1160398 5
CATGC 1101165 1.5150515 5.7453794 2
GCTGT 1515985 1.5087895 7.415456 7
CACGT 1076725 1.4814254 5.33023 95-97
CCTCC 744505 1.4671301 5.041119 1
TACTT 1949105 1.4669834 6.2192745 5
ACGGT 1235950 1.4598422 7.2243257 4
GTATT 2206855 1.4259158 6.424673 4
CTACC 877235 1.4059209 5.115982 20-24
ACATG 1253115 1.4021994 10.728525 1
GGCTG 1103260 1.375527 5.276637 9
AACCA 886680 1.3715982 5.1128197 75-79
ACGTC 992650 1.3657496 5.013783 95-97
AACTA 1285120 1.362311 5.8305693 75-79
GGTAC 1132865 1.3380833 9.708009 3
CAGGT 1125045 1.3288468 5.2232666 95-97
AACGC 813625 1.3285275 6.3999867 95-97
GCGGC 730160 1.328424 6.5472455 7
ACTTT 1733850 1.3049732 6.052916 6
CCGCC 523420 1.2921379 5.926776 45-49
GACGG 870555 1.2881271 9.364997 3
TGTAT 1985835 1.2831081 6.5840955 9
GCCTG 881810 1.2806671 7.434778 3
GGTCA 1080320 1.2760199 13.835467 9
ATTCG 1341580 1.264923 8.606885 6
GTATG 1549725 1.254389 5.179091 7
AGCCT 900665 1.2391909 5.488789 4
CAATT 1362675 1.2171766 11.683404 4
TGGTC 1215950 1.2101786 11.788216 8
ATGGG 1165290 1.1815964 9.786557 3
GTACT 1223940 1.1540048 7.6032314 4
CTGAC 836965 1.1515485 8.452657 1
GTGAG 1133445 1.1493056 5.1692667 8
CTAAC 866895 1.1299397 6.5331945 95-97
TGACG 944940 1.1161158 7.3406057 2
TGGCC 768390 1.1159453 9.67357 1
GGTCG 878725 1.0955803 9.307091 6
AAGCC 667575 1.0900496 6.0704904 1
TGGGT 1242890 1.0619315 6.9818683 4
ATTGG 1306620 1.0576133 10.144348 6
TCGTG 1015810 1.0109885 7.3971233 8
GGGTC 769060 0.95885175 9.157206 5
AATTG 1213300 0.93037826 8.996267 5
CGGTA 769540 0.9089421 6.806943 5
GGCCT 566700 0.8230277 7.005242 2
GGGGT 741050 0.79317474 5.1540365 8
GTCGT 710525 0.70715255 7.152048 7
GCCTA 479470 0.65968466 5.0959706 3
Produced by FastQC (version 0.10.1)