FastQCFastQC Report
Thu 31 Jul 2014
1L22A_ATCACG_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L22A_ATCACG_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 16626290
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 232500 1.3983877341246904 No Hit
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 230801 1.388168978166506 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 148697 0.8943486490371574 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 124272 0.7474427548178217 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 109860 0.6607607590147893 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 106557 0.6408946313338694 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 83015 0.49929960321875777 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 78492 0.47209569904049553 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 78419 0.4716566353648349 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 63333 0.3809208187755657 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 61717 0.37120127220203664 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 59494 0.3578308810925348 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 57252 0.3443462131359431 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 52733 0.3171663672412787 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 52683 0.3168656386963057 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 52074 0.3132027650185339 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 49300 0.29651834534342897 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 49118 0.2954236934397271 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 49003 0.29473201778628905 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 43316 0.2605271530810542 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 42909 0.2580792227249735 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 38930 0.23414724511601806 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 37316 0.22443972768428797 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 35415 0.21300602840441255 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 35314 0.21239855674356697 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 34904 0.20993258267478793 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 34460 0.20726211319542723 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 34010 0.2045555562906698 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 32314 0.19435484404518386 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 30807 0.1852908856996961 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 28437 0.17103635266797343 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 28077 0.16887110714416748 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 27712 0.16667578876586417 No Hit
TGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 26349 0.1584779286298988 No Hit
AGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 26268 0.15799074838704244 No Hit
GGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 25982 0.15627058110979658 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 25828 0.1553443371912796 No Hit
TGACGAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 25144 0.15123037069604825 No Hit
CGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 24100 0.14495115867701092 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 23609 0.14199800436537557 No Hit
TTGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 22298 0.13411290191618214 No Hit
TGGCTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 21528 0.12948168232359714 No Hit
CTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCA 21511 0.1293794346183063 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 21364 0.12849529269608553 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 20741 0.12474821502572132 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 20238 0.12172288586329241 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 19980 0.12017112657123147 No Hit
GGCCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 18070 0.1086832961532609 No Hit
TGGTCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 17427 0.10481592706490744 No Hit
CGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 17122 0.10298148294057184 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 16997315 7.3015227 12.584961 7
AGCAG 6634555 4.8482146 56.47443 20-24
TAGCA 8458820 4.715326 37.7686 20-24
ACTTG 7547045 4.617134 115.536415 8
CACGG 4686435 4.481199 52.388584 45-49
CCTGC 4384480 4.1848826 53.055035 15-19
GCAGA 5687755 4.1563387 39.336002 25-29
CTGCG 3887705 4.0797973 57.671177 15-19
GAAGT 6083945 3.7287846 33.80783 60-64
GTTTC 5385050 3.6155877 39.78065 40-44
TCACG 4950295 3.610893 39.95498 45-49
CGGAT 4452510 3.5708246 40.961617 45-49
AACTT 8048725 3.4226375 80.45574 7
CTTGA 5592370 3.4213023 33.937614 55-59
GAACC 5142225 3.4177554 36.21842 70-74
AGAGA 6091410 3.40178 28.700502 90-94
GCGTT 3783265 3.329845 45.21423 15-19
ACGGA 4530260 3.3104963 39.41302 45-49
AGAGC 4528800 3.3094296 64.955864 95-97
AGTAG 5363150 3.287017 31.746918 35-39
TGCGT 3688415 3.2463627 47.852325 15-19
GAGCC 3358365 3.21129 77.23608 95-97
CCATG 4358005 3.178859 40.61782 75-79
GAGAG 3859290 3.100684 54.566364 95-97
CTTGT 4611015 3.0958912 126.9169 9
ACCTG 4229730 3.0852914 31.270164 15-19
TTCTC 4995810 3.0508091 32.16058 30-34
CATGC 4177165 3.046949 39.90117 75-79
TGAAG 4901520 3.004089 26.157166 60-64
CACTG 4046295 2.9514883 39.360672 85-89
CTCAG 3981115 2.903944 40.014748 35-39
TGCAT 4713125 2.8833976 31.713556 75-79
AGCAC 4324025 2.8739426 33.93333 80-84
AACCA 6194640 2.8618329 24.01658 70-74
GGAAC 3905090 2.8536525 40.05008 70-74
ATAGC 5110560 2.8488555 30.300549 80-84
AAGTT 6039220 2.8235452 26.556087 60-64
GCACT 3866570 2.820392 30.172684 85-89
TTGAA 6013415 2.8114803 24.479544 55-59
TTCAC 5011200 2.7884135 24.567434 45-49
GACTC 3772450 2.7517374 39.533573 25-29
GGATT 4062145 2.732317 28.388884 50-54
ACCAT 5374405 2.7249103 29.55792 75-79
GCAGC 2816885 2.6935234 15.558947 20-24
AAGGA 4754155 2.6549828 23.732418 70-74
AAACT 6838605 2.6497672 43.324497 6
TTTCA 5611255 2.6187105 24.701937 40-44
GTAGT 3866830 2.6009424 28.32635 40-44
CAGAC 3904300 2.5949745 36.219887 25-29
ATGCA 4654375 2.5945575 30.720118 75-79
AGGAA 4547105 2.5393546 30.781607 70-74
CTGAA 4537695 2.5295148 30.00856 85-89
TTGTA 4863700 2.4955962 33.966534 9
TTAGC 4068075 2.4887686 33.818497 20-24
CAGTA 4440590 2.475384 30.39182 35-39
GTTAG 3673500 2.4709027 36.9803 20-24
TCAGT 4035455 2.4688122 33.771893 35-39
GTTAC 3991510 2.4419272 31.2456 60-64
AGAAG 4355255 2.432215 14.865569 6
TAGAG 3956110 2.4246573 32.614864 90-94
TCTCA 4330210 2.409486 23.67225 35-39
AGTTT 4687990 2.4054382 28.56917 40-44
CATTC 4319200 2.4033597 30.794516 30-34
GCATA 4295575 2.3945463 23.451483 80-84
CATAG 4282990 2.3875308 30.39801 80-84
TAAGG 3846735 2.3576226 31.402933 65-69
CTCAT 4183030 2.3275898 23.536453 30-34
AGGAG 2875545 2.31031 16.152792 90-94
TAACC 4547195 2.3055012 27.542171 10-14
CTAAG 4082200 2.2756014 30.202122 65-69
AACCT 4482065 2.2724795 26.341883 10-14
CGTTA 3686840 2.2555366 25.549635 20-24
GTAAC 4038320 2.2511408 30.661177 10-14
TGTAA 4804010 2.2460415 26.238823 10-14
GCTGC 2110025 2.2142818 23.913647 1
ACTGA 3965340 2.2104585 30.405588 85-89
TGAAT 4692835 2.1940634 24.320183 85-89
AGACT 3935240 2.1936793 30.606998 25-29
TCATT 4538665 2.1181445 25.769793 30-34
ATTCT 4524345 2.1114614 25.815458 30-34
TAGTT 4083220 2.0951266 28.119244 40-44
GAATA 4896405 2.0859199 17.941061 90-94
ACTCA 4070505 2.0638118 26.187395 25-29
AGTTA 4406035 2.0599744 25.30949 60-64
GAAGA 3683910 2.057299 7.4745545 50-54
AATAG 4775860 2.0345664 23.39787 90-94
CTGCA 2705545 1.9735053 14.63718 3
TTAAA 6008695 1.9526856 18.210072 50-54
CAGCA 2918310 1.9396409 12.142379 20-24
ACAGG 2652560 1.9383636 11.042747 90-94
GGAGG 1676480 1.9378057 13.863594 90-94
TTACT 4088880 1.9082348 19.604664 65-69
GTTGT 2581485 1.9056314 10.421501 35-39
ACCAG 2855130 1.8976486 10.084653 55-59
GATGA 3028245 1.8559788 8.176798 15-19
AAGAG 3317860 1.8528763 8.217742 50-54
TAAAC 4778140 1.8513949 20.247557 50-54
CCAAA 3997360 1.8467218 35.440517 4
ATTAA 5672370 1.8433878 18.244305 50-54
CAGAA 3613900 1.8356268 10.90728 6
GGAAG 2284185 1.8351916 7.216444 6
GATTA 3899410 1.8231095 25.517035 50-54
GGAGC 1723305 1.8117318 16.001818 95-97
TGTTG 2441510 1.8023027 9.447907 35-39
TGCAG 2214440 1.7759368 16.606981 4
TACTA 4147730 1.7637795 23.263565 65-69
GGGGG 1050835 1.7474633 7.0977707 1
ATAGA 4094165 1.7441573 23.219952 90-94
GTAGG 1974335 1.740863 15.395858 7
CCAGA 2615060 1.7380874 12.544895 55-59
AGGGA 2155885 1.732111 14.11857 9
TCCTC 2347305 1.7090963 9.232082 1
TTTCC 2772025 1.6928024 8.526665 40-44
CAGGC 1765980 1.6886413 11.562944 65-69
CCTCC 1940890 1.6849461 6.2497854 1
ACTAA 4332275 1.6786348 21.399603 65-69
TGACC 2300260 1.6778781 50.403385 1
GGCTC 1596990 1.6758974 14.629223 65-69
ACCAA 3534450 1.632864 18.224907 3
CCATC 2455355 1.6289873 9.002544 75-79
TGATG 2350415 1.5809574 9.801314 30-34
AGCTG 1955025 1.5678912 6.0876107 1
AAGAA 4029710 1.5642295 7.5993547 45-49
GGATG 1747475 1.54083 12.890748 10-14
CAAAC 3333035 1.5398133 41.333584 5
GAGCT 1896140 1.5206666 7.341366 95-97
TTGTT 2698555 1.519611 8.514233 35-39
GAGCG 1439975 1.5138632 7.4488425 90-94
TAGGG 1705535 1.5038495 15.3599415 8
CAGAG 2057615 1.5036063 10.236148 25-29
AGCCA 2234015 1.4848274 15.12157 95-97
GATGT 2201930 1.4810822 11.595956 30-34
TGCTG 1681165 1.4796792 5.489828 1
CAGGA 2011270 1.4697394 9.723449 90-94
GACCA 2203155 1.4643165 23.51515 2
TTCCG 1809435 1.4485055 10.83245 40-44
GAGGA 1796095 1.4430435 14.297585 95-97
ATGAT 3026490 1.4149892 6.9864926 15-19
CATCA 2781085 1.410055 6.4681554 80-84
AACAG 2774855 1.4094464 11.256338 60-64
GAGAT 2291990 1.4047359 10.705819 25-29
TGTTT 2468120 1.3898485 10.037248 35-39
GAGAA 2426870 1.3552982 9.991801 50-54
GGCCA 1414225 1.3522909 21.68169 2
GCTCA 1852180 1.3510354 12.555559 65-69
CTACA 2643560 1.3403276 7.805259 85-89
AGGCT 1659895 1.3312029 10.86536 65-69
TACAG 2384920 1.3294615 7.9939446 90-94
GAAAC 2616765 1.3291469 7.1554112 60-64
TCCAA 2619305 1.32803 5.3479238 3
AAAGA 3420600 1.3277887 6.110312 45-49
GCCAA 1994675 1.3257513 17.703783 3
CAACA 2844135 1.3139488 6.7635183 8
AGAAA 3382430 1.3129721 5.3110394 60-64
TCTTG 1933330 1.2980613 6.517086 4
TCCGT 1618645 1.2957726 11.193792 40-44
GGGAT 1463740 1.2906475 14.969265 10-14
AGCGT 1593620 1.2780516 5.411038 90-94
GCTGG 1104480 1.2743338 5.864657 1
TACCA 2499250 1.2671602 7.2250957 75-79
CTCTT 2066670 1.2620605 6.734312 3
CGGAA 1718330 1.2556729 6.0991735 5
CTCAC 1885465 1.250898 8.839713 65-69
ATACC 2450745 1.2425673 6.8794813 75-79
ATGTT 2386005 1.2242746 6.960341 30-34
TAAAG 2843450 1.2113396 6.6060276 45-49
TGGCC 1129870 1.185697 34.445915 1
AAGTA 2778585 1.1837064 16.122847 8
GCCCA 1360690 1.1833977 7.5944276 2
AGATG 1927300 1.1812215 8.227282 25-29
CCTCT 1602450 1.16676 7.7056932 2
GTAAA 2735225 1.1652346 5.930231 45-49
CCCAA 1832445 1.1077474 8.208911 3
AGAAC 2180790 1.1076999 7.7910748 50-54
CCAAC 1829890 1.106203 5.1936827 5
GATAC 1982165 1.1049477 6.4914055 75-79
TGCCA 1503240 1.096508 5.2629404 1
TCTAC 1961400 1.0913942 8.955134 85-89
AAACA 3088835 1.0905392 5.4303164 60-64
CAATA 2797995 1.0841444 6.597872 80-84
CCGAA 1627810 1.0819162 34.682537 4
TCAAT 2526280 1.0742745 5.86163 80-84
TCAAC 2109390 1.0694948 7.5506516 7
ATCAA 2739665 1.0615431 5.3876433 80-84
CCGTA 1415190 1.0322819 12.300969 40-44
TTGAC 1637975 1.0020808 6.401846 6
TGATA 2137290 0.99925727 6.910534 15-19
GAACT 1786780 0.99603134 29.936644 6
GATAG 1615080 0.9898652 10.452602 15-19
CCATA 1943140 0.9852034 5.2177925 3
GGTGC 850010 0.9807299 8.751187 2
GTCCA 1333680 0.9728259 5.881494 2
CTCCG 998625 0.95316386 5.2500396 2
GTGCA 1186715 0.95172185 6.7544837 3
CGAAC 1407730 0.93564105 33.011074 5
CGTAA 1657085 0.92373353 7.3075647 40-44
GACCT 1262635 0.92100364 11.16171 2
GGATA 1500585 0.91969246 7.877198 70-74
GGGTG 723330 0.91757584 10.508799 1
TAAGT 1950950 0.9121369 5.835454 5
GGCTG 787855 0.9090162 7.2900896 1
CTAAA 2341165 0.9071356 6.946974 4
TGTCC 1124035 0.89982283 9.245998 1
TATCT 1900990 0.8871709 6.087946 85-89
ATCTA 2071280 0.8807905 5.868096 85-89
AACAA 2476940 0.8745045 5.5272684 9
CCTAA 1690460 0.85709053 10.595448 4
GCTGT 970300 0.8540107 6.013869 1
CTGTC 1053075 0.8430173 8.963864 4
AAGTC 1509925 0.84169996 7.054318 6
GGGGT 662505 0.8404165 5.3350368 1
TGCCC 879470 0.83943325 7.9111867 1
ACCTA 1655415 0.83932215 7.797046 3
TGTCA 1364405 0.83471626 7.5001917 5
CCCCC 803470 0.83165413 5.7544947 1
TGGCT 936500 0.82426155 7.2268076 1
GTCAA 1461355 0.81462485 7.860062 6
GCCAG 850355 0.8131148 5.2321835 2
ACCGA 1221320 0.8117445 21.030096 3
GGCCT 760315 0.7978823 6.30326 2
CGCCC 695345 0.79132247 5.10698 1
GGGGC 519415 0.7856116 5.0399203 1
CGTCC 809785 0.77292067 6.1442423 1
ATATC 1795305 0.763435 5.0143986 85-89
GGGCC 553495 0.76142484 9.103894 1
CCGTC 796340 0.7600877 5.8305254 4
AGTCT 1240725 0.75905126 9.040804 7
CGACC 850290 0.73950076 8.616838 1
GGCCG 526520 0.7243163 20.575191 2
GACCG 743540 0.7109777 29.726671 2
CCCCG 607005 0.69078904 5.882543 2
CGGCC 548150 0.6858556 5.807396 1
GCCGA 696190 0.66570127 14.847718 3
TCTAA 1554515 0.6610415 6.645744 9
TGCGG 564855 0.6517219 9.059726 3
CTAAC 1279875 0.6489173 9.709179 5
GACTG 806020 0.646412 8.5968075 2
GCCCC 566510 0.6447046 6.0225697 1
TGACT 1044660 0.6391025 12.236471 1
TCCCG 648145 0.618639 5.0347033 2
GGCCC 490015 0.613116 6.679264 1
TAACT 1424155 0.6056073 8.488794 6
CCCGA 694250 0.60379213 5.6070576 3
GACTT 986385 0.603451 8.572196 6
GTCTA 985195 0.6027229 9.047633 8
TCCGA 823820 0.6009189 5.1787 3
GACCC 660285 0.5742526 6.1568117 2
GCCCG 443665 0.55512196 6.127676 2
GCGGA 514725 0.54113674 7.517139 4
GGACC 560620 0.5360684 5.273322 1
GTCCG 491760 0.5160579 6.6571174 2
CCCGT 533880 0.50957584 5.7349544 3
GACTA 716015 0.3991389 6.030421 2
Produced by FastQC (version 0.10.1)