FastQCFastQC Report
Thu 31 Jul 2014
1L22A_ATCACG_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L22A_ATCACG_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 16626290
Filtered Sequences 0
Sequence length 101
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 326394 1.9631198541586847 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 281726 1.6944610012215593 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 175549 1.0558519068294852 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 144276 0.8677582310906403 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 125169 0.7528378249146382 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 100165 0.6024494941445145 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 95671 0.5754200125223367 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 86462 0.5200318291091999 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 84524 0.5083755907060444 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 82579 0.49667725030659277 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 68878 0.4142716144130771 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 60931 0.36647381947506025 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 58809 0.353710900026404 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 54662 0.32876847450633906 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 54520 0.3279144054386156 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 53440 0.32141866886719767 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 46530 0.2798579839519219 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 45880 0.27594851286727223 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 45002 0.27066771961754543 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 42763 0.2572010953736522 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 42185 0.2537246733937637 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 41540 0.24984527516361135 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 40361 0.2427540960731468 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 38447 0.23124220737157836 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 37978 0.22842137361973117 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 37910 0.2280123827985678 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 37663 0.22652678378640093 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 37101 0.22314659494090386 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 36777 0.22119787396947843 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 36050 0.21682528092557027 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 35313 0.21239254217266748 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 35138 0.21133999226526182 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 33068 0.1988898305033775 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 30840 0.1854893665393783 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 30821 0.18537508969228852 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 30292 0.18219338168647364 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 29405 0.1768584572986517 No Hit
CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA 28117 0.1691116899801459 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 27935 0.168017038076444 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 27230 0.16377676559232396 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 26777 0.16105216497486813 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 26099 0.15697428590503354 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 25983 0.15627659568069605 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 25497 0.153353514223558 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 23685 0.1424551117537346 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 22820 0.1372525079257008 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 22062 0.13269346318390934 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 21562 0.12968617773417881 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 21486 0.1292290703458198 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 21412 0.12878399209925967 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 21108 0.1269555625458235 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 21052 0.1266187465754537 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 20760 0.12486249187281108 No Hit
ATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGGAT 20366 0.12249275093842342 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 20339 0.12233035752413797 No Hit
GCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCT 19672 0.11831863873419746 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 19478 0.11715181197970202 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 19242 0.11573237324742922 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 18284 0.10997041432574556 No Hit
GGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGGATCG 17900 0.10766081910035252 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 17632 0.10604891409929697 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 17029 0.10242212784692195 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 6102495 3.754011 6.1600513 45-49
CTGCT 5251975 3.5369098 11.345955 85-89
ACTTC 5184040 3.390458 7.029356 60-64
AACTT 6039185 3.2350385 6.1124973 60-64
AGCTG 4344445 2.9217696 8.656344 3
CTACT 4333355 2.8340945 9.553984 85-89
TTCAA 5252650 2.8137116 8.721593 9
CTTCA 4282135 2.8005955 7.1462674 60-64
CAACT 3541390 2.7425325 11.385769 9
TCAAC 3441950 2.665524 11.011571 8
TGCTA 4826760 2.662368 6.5846486 1
GCTGC 3233785 2.6552887 10.882378 3
GGAAG 3936915 2.644101 6.3676715 15-19
TCAAG 3981810 2.6006439 5.6101937 10-14
CTTCT 4532425 2.5034142 6.100783 20-24
TCTCT 4528080 2.5010142 8.794542 20-24
ACCCA 2219390 2.4847836 43.641354 1
GCTAC 3114450 2.4835374 11.97067 1
CTGGT 4339265 2.4645624 12.464044 1
CAAGC 2596090 2.4513035 7.9750633 75-79
ACAAC 2660445 2.4396095 8.152505 85-89
ACTGC 3005350 2.3965383 6.4781837 85-89
AGGAA 3623205 2.363217 6.4660306 10-14
TCCTT 4232695 2.337863 9.776214 40-44
TATGC 4228770 2.3325257 8.145386 30-34
AAGCT 3570825 2.3322167 5.837628 15-19
CAGCA 2448600 2.3120391 7.517924 2
ACATC 2972635 2.3020759 7.046131 90-94
GGTGG 3941050 2.301745 8.633525 7
CAACA 2504535 2.296641 7.772259 85-89
TGGTG 4780545 2.289936 8.500756 6
GAAGC 2842785 2.2638292 7.185573 15-19
CGGTG 3256445 2.2551045 14.204474 9
CTGAA 3446285 2.250876 6.2445626 80-84
CATGG 3334865 2.2427967 23.016188 2
CAGCT 2800920 2.233521 8.048408 65-69
GGTGT 4662770 2.2335207 12.904876 1
CCAAG 2310090 2.181254 7.7766414 75-79
GCAGC 2221940 2.160334 7.8623915 1
GCTCC 2182360 2.1247346 9.064246 65-69
CAAGG 2649385 2.1098166 6.8023477 10-14
CTGCA 2635925 2.10195 5.0162354 95-97
TGAAA 3903730 2.088292 5.063747 65-69
CTTGG 3662640 2.080261 7.220724 2
CTGTT 4389270 2.044639 5.1078134 55-59
CTCCT 2527770 2.018443 10.217532 70-74
TCTGC 2989380 2.0131793 5.616617 85-89
CGTGA 2972045 1.9987892 20.488056 9
GCTGG 2882050 1.9958341 9.951738 4
CTCTC 2484400 1.9838121 5.8606997 15-19
TATTC 4380790 1.9818262 8.567675 5
AGCTC 2465865 1.9663401 7.697142 65-69
GTTCC 2917335 1.9646612 5.1557817 70-74
CCTTA 2995100 1.958851 8.552638 95-97
TAGCT 3550705 1.9585155 6.57472 2
TGGCT 3430685 1.9485184 6.4127855 9
CACAA 2121200 1.9451257 7.3753858 85-89
TTCCA 2949445 1.9289918 5.8971553 75-79
TTTCA 4254675 1.924773 7.520671 8
TGCTG 3361990 1.9095017 5.332057 85-89
GCTTC 2811065 1.8930942 8.413077 15-19
CCCAA 1690295 1.8924198 44.28156 2
AAGGA 2875725 1.8756769 6.255527 10-14
CTCTG 2767040 1.8634458 7.1024384 20-24
CCAGC 1608315 1.854118 8.041012 1
CCAAT 2352165 1.82157 30.788282 3
CATCC 1918230 1.8137094 7.4505987 90-94
GGCTC 2204910 1.8104706 5.526106 10-14
CCGTG 2200425 1.8067878 5.9200034 60-64
CTCTA 2742990 1.7939662 6.6420574 95-97
TACTG 3242185 1.7883404 5.313158 85-89
GCTGT 3109980 1.7663683 5.4052453 90-94
CACCC 1290855 1.7644988 8.416408 50-54
GGTAT 3791075 1.7635881 5.3717737 30-34
GTTGC 3095245 1.7579993 6.192407 75-79
CTACC 1852260 1.751334 7.9237704 25-29
GATCG 2604075 1.7513183 6.595574 50-54
TTCAG 3131915 1.7275172 5.9594626 65-69
CTTTC 3123430 1.7251776 8.932378 7
CCTGT 2546915 1.715204 7.2507725 4
GTGGC 2442165 1.6912113 7.353698 8
GCACC 1462360 1.6858563 7.3050427 50-54
TTCGG 2954135 1.6778533 11.550125 7
GAACA 2144100 1.6581866 6.6685605 80-84
GCTCT 2460830 1.6572306 5.1208873 10-14
TCGGT 2913155 1.654578 13.4847355 8
GCAGG 2015380 1.6526005 5.419819 90-94
CCACA 1467565 1.6430558 7.161466 60-64
ACACA 1777795 1.6302255 7.1268387 85-89
AATGA 3028960 1.6203357 5.9935265 75-79
TTCTT 4226780 1.6148597 5.5715733 95-97
GGAGG 2330070 1.6113958 5.2106996 7
ACATG 2458765 1.6058958 22.9616 1
TCCAA 2061650 1.5965884 6.2313037 75-79
GCGGC 1567480 1.5692852 7.960801 75-79
TCAGC 1960985 1.5637366 6.3027525 65-69
TTGGT 3978910 1.5631866 16.272097 7
TGAAC 2380225 1.554599 5.9435444 80-84
GTGTA 3336470 1.5521078 12.171972 2
GCCTT 2300585 1.5493146 5.241595 35-39
AACAC 1687860 1.547756 7.1491594 85-89
CAACC 1377260 1.5419521 5.1213408 75-79
GCTGA 2285570 1.5371143 5.574892 80-84
TTAGG 3293065 1.5319163 6.384646 30-34
TACTT 3385685 1.5316505 8.8209 3
GCTTG 2696255 1.5313857 7.178086 1
TGTAG 3256710 1.515004 6.0488396 9
GGCTG 2164050 1.4986155 6.657948 80-84
AGAAA 2354335 1.4913092 5.069869 9
GGGAA 2217835 1.4895368 5.8499784 15-19
ACGGT 2213505 1.4886484 6.58895 4
CATGC 1860470 1.4835835 5.7216597 2
GGAAC 1861335 1.4822594 6.665849 15-19
ACTAC 1892905 1.4659084 5.3951645 70-74
CCGCC 1036635 1.4590867 6.3308806 45-49
GCAAG 1826505 1.4545226 5.616256 1
GTAGC 2161615 1.453751 7.5120244 1
AACAT 2290405 1.452785 5.078037 90-94
TGCAC 1816945 1.4488757 5.2306128 50-54
TGGGA 2543775 1.4428216 6.0649376 15-19
AACCA 1568710 1.4384961 5.310905 75-79
GCAAC 1513115 1.428727 5.8293514 1
TCCTG 2115485 1.4246602 5.6198215 70-74
CGCAG 1456325 1.4159467 6.2969604 90-94
TTCAT 3116585 1.4099123 5.000194 60-64
ATGCC 1767050 1.4090883 7.1091223 9
CATGA 2153015 1.406201 5.1695 65-69
AATCC 1810810 1.4023322 5.2664595 60-64
TGGTA 3007205 1.3989356 9.002352 2
ATGGG 2458670 1.3945506 19.911201 3
GAACT 2134405 1.3940463 5.462024 15-19
AGGTA 2525925 1.3913716 6.3944817 35-39
ACTTT 3071425 1.3894823 9.015891 4
CTGTA 2471320 1.3631428 6.9968004 8
GGTCG 1944355 1.3464758 20.913414 6
CAATT 2508520 1.3437506 21.349857 4
GGTAC 1993420 1.340635 11.341527 3
CACAT 1711995 1.3258078 5.022 60-64
AGCTA 2027340 1.3241189 5.7903996 20-24
GTCAA 2026315 1.3234494 5.283088 10-14
GTATT 3465230 1.3221098 7.641164 4
ATCTC 1964650 1.2849176 5.64759 45-49
TAGGT 2736960 1.2732193 5.287044 30-34
GACGG 1551455 1.2721846 8.45696 3
GTCTG 2238675 1.271495 5.1166854 90-94
TTCTC 2276850 1.2575827 5.285717 20-24
CAGGT 1862010 1.2522575 5.571836 95-97
TGGGT 2610145 1.2502896 15.116651 4
TGGTC 2194075 1.2461637 19.867544 8
CGCTG 1513725 1.2429326 6.7722774 50-54
GGGTC 1777945 1.2312359 20.861828 5
AAGCC 1301915 1.2293059 6.817581 2
GAATC 1875875 1.2251924 5.150516 40-44
ATCCT 1870030 1.2230344 5.151866 90-94
GTGAG 2145770 1.2170745 5.9822006 10-14
GGTCA 1805410 1.2141925 23.28378 9
GTGGG 2063555 1.205206 5.7798944 10-14
TCGCT 1787555 1.2038177 5.4943266 50-54
GTACT 2171865 1.197968 9.224693 4
CCTTG 1774320 1.1949047 6.507859 45-49
TGTAT 3131155 1.1946481 7.652034 3
ATTCG 2159835 1.1913325 10.301493 6
TGCGG 1706625 1.1818464 5.595197 75-79
AGCCT 1472135 1.1739159 5.951808 4
TGCCT 1742780 1.1736643 5.166342 30-34
AACGC 1241855 1.1725955 5.4553976 95-97
TCGTG 2061140 1.1706609 17.238049 8
CTGAC 1454950 1.1602122 7.7156425 1
AGTGG 2029980 1.1513988 5.1295013 10-14
TGGCC 1389220 1.1407005 11.186578 1
ACTTA 2115665 1.133308 5.28483 20-24
GCCTG 1378420 1.1318325 8.250474 6
TGACG 1669590 1.1228492 6.636927 2
CGGCT 1351930 1.1100813 6.5539265 75-79
GCTCA 1382560 1.1024866 6.375002 1
CGTCC 1115640 1.0861815 7.3688 40-44
GAGTG 1912310 1.0846566 5.232824 10-14
CCGTT 1606860 1.0821298 5.554076 25-29
GTCCT 1605790 1.0814092 5.419773 40-44
GGATC 1595620 1.0731024 5.7265873 45-49
ATTGG 2305135 1.0723364 16.869646 6
ACCGT 1343175 1.07108 6.1746893 25-29
TCTTG 2292080 1.067712 6.1004496 95-97
CTTAT 2312390 1.0461024 5.6713867 95-97
CCAGG 1064290 1.0347812 5.2365665 1
TTGGC 1818780 1.0330083 5.7456484 3
TACAT 1927425 1.0324727 5.862227 4
AATTG 2229125 1.0070658 15.904162 5
TGGGG 1719800 1.004438 5.053114 8
GCGTC 1199810 0.9851743 6.171576 40-44
ATCGC 1229645 0.9805485 6.075932 50-54
ATGCG 1434595 0.9648083 5.469231 35-39
TACCG 1198350 0.95559317 6.0258207 25-29
GGGGT 1562035 0.91229653 5.1629586 8
GGCCA 932125 0.9062807 5.229968 1
CGGTA 1342965 0.9031842 6.1693983 5
TGCGT 1585975 0.9007826 5.2773542 40-44
GCCCA 764770 0.8816518 5.167836 2
GTCGT 1522080 0.8644923 17.00789 7
CTACA 1116095 0.86432934 8.821427 3
GCATA 1320185 0.86225384 5.9287724 60-64
GGCCT 1031095 0.84664094 7.9797373 5
GCCTA 921035 0.73445547 5.039208 4
GGCCC 618100 0.73373 6.111475 1
GCCCT 726635 0.70744807 5.6826215 1
CCCCG 497355 0.70003814 5.89676 1
CCCTA 686950 0.64951944 5.0089808 2
GGTAA 1017080 0.5602448 5.9160748 7
Produced by FastQC (version 0.10.1)