FastQCFastQC Report
Wed 6 Aug 2014
1L15C_ACAGTG_L004_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L15C_ACAGTG_L004_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14012045
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 248762 1.7753439986811348 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 221296 1.5793269290813725 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 130821 0.933632456932589 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 116249 0.8296362165551139 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 93550 0.667639876977272 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 79079 0.5643644450185537 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 72920 0.5204094049084199 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 63488 0.4530958900003533 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 62012 0.4425620956826787 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 61942 0.44206252549146113 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 51333 0.36634909465392096 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 46189 0.32963782231644273 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 42839 0.3057298203081706 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 40510 0.2891084063746584 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 38543 0.27507048400144307 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 37204 0.26551441991515157 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 33014 0.23561157561226787 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 31276 0.2232079614360359 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 31134 0.22219454761956586 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 29549 0.21088285114699534 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 29090 0.20760709803601113 No Hit
GCTGCTGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 28604 0.20413865356555733 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 27103 0.19342644132244793 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 26071 0.18606134936049662 No Hit
GCTGCCGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 24670 0.17606280881912667 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 24103 0.1720162902702639 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 23290 0.16621413933512202 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 22423 0.16002660568104085 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 22259 0.15885618409018812 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 22060 0.15743597740372656 No Hit
GCAGAGGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 21688 0.15478111867325575 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 20822 0.14860072173619196 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 20110 0.14351937921980695 No Hit
GCAGATGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 19577 0.13971550904953559 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 18501 0.13203640153881893 No Hit
GCAGTAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 18315 0.13070897217358352 No Hit
ACAGGGGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 17460 0.12460707912371108 No Hit
GCTGTGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 17426 0.12436443074511963 No Hit
CGTCCCATATTTAAGTCGTCTGCAAAGGATTCATCTCCCCGATCGTCTGG 17345 0.12378635666671067 No Hit
GCAGACGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 16328 0.11652831546002029 No Hit
GCAGGAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 15247 0.10881352436421664 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 14693 0.10485978313657998 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 14582 0.10406760754764917 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 14573 0.10400337709449263 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 14389 0.102690221163292 No Hit
GCAGCAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 14209 0.10140561210016097 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AGCAG 5982830 4.8859572 54.892063 20-24
TTTTT 7811165 4.477473 8.166367 7
TAGCA 6559110 4.259657 34.092617 20-24
CACGG 3797950 4.193535 40.588867 45-49
ACTTG 5369935 3.9926536 98.393425 8
GCAGA 4816635 3.9335685 35.41327 25-29
GTTTC 4454535 3.7919092 35.46283 40-44
CCTGC 3150835 3.740515 44.664146 15-19
TCACG 4045335 3.5519938 32.38879 45-49
CTGCG 2740225 3.464018 46.74941 15-19
CGGAT 3602310 3.3681154 32.15491 45-49
GAAGT 4802135 3.320873 32.04286 7
AGAGA 5440530 3.2862165 20.37649 90-94
GAACC 4265700 3.2714863 28.29154 70-74
AGTAG 4515680 3.1227775 32.001526 9
CTTGA 4194465 3.1186683 28.069767 55-59
GCAGC 2807640 3.1000767 19.816957 20-24
AGAGC 3792035 3.0968153 47.74036 95-97
ACGGA 3691755 3.0149205 29.676352 45-49
AACTT 5783470 2.9867916 68.51507 7
TTCTC 3626290 2.8988779 28.57402 30-34
CTTGT 3329860 2.834533 112.78996 9
CCATG 3187385 2.7986736 32.84273 75-79
GCGTT 2607530 2.7912462 37.2203 15-19
AACCA 5195990 2.7678778 18.790054 70-74
TGCGT 2564210 2.744874 39.360416 15-19
ATAGC 4213090 2.7360904 23.246033 80-84
ACCTG 3100680 2.7225425 26.43078 15-19
ACCAT 4438695 2.7070534 30.225267 75-79
CATGC 3044815 2.6734903 32.171986 75-79
TTCAC 3799875 2.6532233 21.91551 45-49
GCTGC 2077235 2.6259084 39.81619 1
GAGCC 2331015 2.5738077 56.37791 95-97
TGCAT 3457850 2.5709803 25.994316 75-79
TTTCA 4334320 2.562715 21.4283 40-44
AGGAG 2919285 2.5386782 20.621067 90-94
AGAAG 4121270 2.4893503 17.117785 6
TGAAG 3595635 2.4865286 20.46978 60-64
AAGTT 4512950 2.4817903 21.013828 60-64
CACTG 2819385 2.4755523 30.718193 85-89
CTCAG 2815845 2.472444 32.37839 35-39
GTAGG 2480885 2.4700215 28.141554 7
GAAGA 4034260 2.436794 9.440118 50-54
TTGAA 4404610 2.4222116 19.515167 55-59
GAGAG 2780015 2.4175658 39.02806 95-97
AGCAC 3130195 2.4006352 25.844498 80-84
GCACT 2706155 2.376131 24.365507 85-89
ACAGG 2893530 2.3630395 14.459647 60-64
TTGTA 3726640 2.3463078 25.728376 9
AAGAG 3867680 2.3361757 10.267997 50-54
GACTC 2652565 2.3290765 32.19899 25-29
GGATT 2918290 2.3105178 23.117613 50-54
GGAAC 2825895 2.3078043 29.955675 70-74
GATGA 3266730 2.2590773 10.497146 15-19
GTTGT 2484860 2.2524009 14.710207 35-39
AAACT 4886455 2.2041817 30.088125 6
CATTC 3149710 2.1992521 26.09723 30-34
GTAGT 2775580 2.1975286 23.118038 40-44
ATGCA 3376980 2.1930988 24.140072 75-79
TGTTG 2408575 2.1832523 13.507083 35-39
TTAGC 2933865 2.181387 27.761965 20-24
AGTTT 3463095 2.180379 23.635918 40-44
GGAAG 2495665 2.1702883 12.039829 6
ACCAG 2826775 2.1679337 13.288569 55-59
CTGAA 3314135 2.1522856 22.797832 85-89
CAGTA 3308065 2.1483436 23.975792 35-39
TCTCA 3076115 2.147865 20.682411 35-39
CAGCA 2797625 2.145578 17.214138 20-24
TCAGT 2883220 2.1437314 27.601173 35-39
TTTCC 2667035 2.1320436 12.592326 40-44
GGCTC 1680950 2.1249502 19.935184 65-69
GTTAC 2857190 2.124378 25.616945 60-64
CTGCA 2418550 2.1236002 17.814638 3
AGGAA 3511215 2.1208618 22.343887 70-74
CTCAT 3006715 2.0994072 20.591995 30-34
TAGGG 2104465 2.09525 28.102337 8
AAGGA 3463170 2.0918415 17.808352 70-74
CAGAC 2726430 2.0909765 28.118565 25-29
GGAGG 1664920 2.0844946 17.607746 90-94
TGTAA 3763180 2.0694723 20.838833 10-14
AGGGA 2371550 2.0623553 24.720736 9
GCATA 3165260 2.0556023 18.87973 80-84
GTTAG 2595165 2.0546875 29.410576 20-24
TCATT 3456985 2.0439808 22.03581 30-34
CCATC 2444360 2.0155551 14.39759 75-79
CATAG 3088515 2.005762 23.620188 80-84
CAGGC 1807835 1.9961346 15.613876 65-69
CAGAA 3518085 1.9955982 12.778262 5
GATCG 2134120 1.9953759 7.115856 95-97
TAACC 3267130 1.992544 22.419308 10-14
GCGTC 1561580 1.97405 7.1804047 95-97
AACCT 3233530 1.9720525 21.48206 10-14
TTCCG 1958605 1.9689175 15.71755 40-44
CCAGA 2561295 1.9643298 17.681591 55-59
TAGAG 2827865 1.9555843 23.727564 90-94
ATTCT 3280795 1.9398065 22.243452 30-34
TAAGG 2777140 1.9205059 23.823236 65-69
CGTCG 1513785 1.9136306 7.464743 95-97
GGAGC 1626915 1.912864 18.113337 95-97
CGTTA 2568215 1.909519 21.594313 20-24
GAGCG 1623650 1.909025 8.352979 90-94
GTAAC 2933775 1.9052699 24.04489 10-14
TTGTT 2633130 1.8980287 12.280694 35-39
CTAAG 2918075 1.8950739 23.694204 65-69
TGAAT 3407560 1.8739072 18.577267 85-89
TAGTT 2974590 1.872814 23.174122 40-44
TGCAG 1991375 1.861911 19.412409 4
ATCGG 1987370 1.8581665 6.390754 95-97
AGTTA 3378235 1.8577808 20.065397 60-64
GGATG 1844255 1.8361794 18.599617 10-14
ATGAT 3335830 1.834461 9.2447195 15-19
GATGT 2298985 1.8201909 17.065706 30-34
TTAAA 4753540 1.815706 14.575076 50-54
TCGGA 1941625 1.8153955 6.0434732 95-97
ACTGA 2780875 1.8059726 22.952312 85-89
AGACT 2770155 1.7990108 24.013039 25-29
GAATA 3732150 1.7926718 13.656062 90-94
TGATG 2250210 1.781574 13.063869 30-34
ACTCA 2919645 1.7806213 21.364311 25-29
TGTTT 2459710 1.7730231 15.90368 35-39
AGATC 2722320 1.7679454 6.10956 95-97
TTACT 2983210 1.763856 17.227247 65-69
AGCTG 1883890 1.7614139 8.777271 95-97
GAGAT 2530880 1.7502072 15.038678 25-29
AATAG 3622600 1.7400514 17.259172 90-94
TGACC 1981240 1.7396216 62.451942 1
CAGAG 2127580 1.7375163 13.089769 25-29
TCCGT 1724810 1.7338915 16.091871 40-44
CCTCC 1547215 1.7249172 8.722596 1
CGGAA 2093935 1.7100395 10.093042 5
CCAAA 3204485 1.7070131 27.851334 4
GTCGT 1584575 1.6962178 6.460745 95-97
TCCTC 1792005 1.6917324 6.944789 1
ATTAA 4397930 1.679874 14.487037 50-54
AGCGT 1772165 1.6569525 6.325795 90-94
AGGCT 1771380 1.6562186 14.486823 65-69
CAGGA 2022425 1.65164 11.25198 90-94
GCTCA 1860660 1.6337466 18.59333 65-69
AAGAA 3866520 1.6221799 9.9526005 45-49
CATCA 2650740 1.6166227 8.447777 80-84
ACCAA 3014135 1.6056144 17.773428 3
TAAAC 3536780 1.5953704 15.99467 50-54
GGGAT 1594265 1.587284 21.245184 10-14
TCTCC 1674620 1.5809157 5.2826543 1
TACTA 3049260 1.5747472 18.952639 65-69
GAGCT 1679040 1.5698818 10.065552 95-97
GATTA 2849835 1.5671996 20.077854 50-54
CTACA 2544745 1.5519788 10.23126 85-89
CTCAC 1881115 1.5511181 14.109856 65-69
CCACC 1590375 1.5486534 6.370908 1
GAGGA 1769650 1.538929 16.02864 95-97
ATAGA 3139965 1.5082263 17.083591 90-94
TACCA 2472910 1.5081683 9.630439 75-79
AAAGA 3587530 1.505131 7.4253182 45-49
GGGGG 834945 1.5050172 5.940731 1
ATGTT 2366080 1.4896936 11.186726 30-34
TAAAG 3089305 1.4838927 8.610144 45-49
TCGTG 1385305 1.4829081 5.6987844 95-97
GTAAA 3079340 1.4791062 7.8662434 45-49
TACAG 2268980 1.4735347 8.990376 90-94
ACTAA 3217080 1.4511602 16.747847 65-69
CTGCT 1439855 1.4474362 6.602735 2
TGCTG 1351780 1.4470211 6.3768296 1
AACAG 2548820 1.4457924 13.744963 60-64
GAAAC 2532800 1.4367051 9.081137 60-64
ATACC 2351485 1.4341142 9.287308 75-79
GAGAA 2369425 1.431192 13.257533 50-54
CCGTA 1594350 1.399914 18.559715 40-44
TCTAC 2002110 1.3979523 13.058483 85-89
AGAAA 3315645 1.391063 6.5839663 60-64
AGATG 2010985 1.3906784 12.148827 25-29
GCTCC 1142390 1.3561885 6.4748845 1
GACCA 1731355 1.3278253 23.501831 2
AAGTA 2750180 1.321 23.142427 8
CGTGT 1233835 1.3207661 5.194214 95-97
AGCCA 1692995 1.2984059 10.067783 95-97
CAAAC 2397765 1.2772772 29.748655 5
AGAAC 2241650 1.2715533 10.970392 50-54
GATAC 1914455 1.2432969 8.656165 75-79
CAATA 2743535 1.2375536 8.797644 80-84
AAACA 3132680 1.2342598 6.798757 60-64
TCAAT 2373575 1.2257993 7.529834 80-84
GCTGG 895570 1.20554 6.213682 1
GATAG 1734600 1.1995468 14.78933 15-19
CTTTT 1765335 1.195004 5.164568 6
CGTAA 1834285 1.1912324 11.229107 40-44
TGATA 2155270 1.18524 9.192663 15-19
CCTCT 1247815 1.1779928 5.7345047 2
GCCAA 1534240 1.1766521 11.687138 3
ATCAA 2605425 1.175255 6.7884393 80-84
CTCCC 1026065 1.1439116 5.129953 1
CCTTT 1426605 1.1404365 5.5114117 4
GGCCA 1032020 1.1395127 14.485805 2
GCCCA 1096755 1.1372408 6.1407137 2
CCGAA 1473230 1.1298618 31.502556 4
CCCAA 1565030 1.1271695 9.849589 4
GTGCT 1048915 1.1228174 6.922634 9
GGATA 1615865 1.1174369 11.446369 70-74
TATCT 1881330 1.1123573 8.248532 85-89
CCAAC 1541890 1.1105037 7.7205186 5
TTGGT 1220750 1.1065485 5.7118917 7
ATCTA 2112330 1.0908829 7.532615 85-89
TGGCC 857655 1.0841929 28.996693 1
CCCTT 1115910 1.0534687 5.091959 4
AATAT 2708345 1.0345045 6.107672 80-84
GTTGC 962580 1.0303996 5.618796 1
TCAAC 1643155 1.0021207 5.032212 7
CAACT 1639435 0.9998521 6.615462 6
CCCTC 879685 0.9807194 5.091561 1
CCATA 1606195 0.97957975 5.064442 3
CTCCG 814645 0.9671061 6.7995353 2
GGTGC 715130 0.962647 8.62342 2
ATATC 1831340 0.9457696 6.593199 85-89
CTGCC 788125 0.9356229 6.2496533 2
ACCCA 1296850 0.93402034 6.892499 3
GTGCA 997225 0.9323931 6.5959992 3
GGGTG 649620 0.9311716 9.912787 1
CCGCC 662630 0.92897046 5.683974 1
TGGTC 867405 0.92851895 6.1693745 8
CCCAT 1110835 0.9159654 5.5133605 4
GAACT 1396925 0.90719944 26.774025 6
ACCGA 1175335 0.9013977 21.591763 3
GACCT 1019360 0.8950459 12.036819 2
GCTGT 835395 0.8942536 8.380946 1
TGTGC 834910 0.8937345 6.8114877 8
GGCTG 662490 0.8917876 7.4561586 1
CGAAC 1157010 0.8873438 29.247095 5
CCTAA 1450535 0.8846464 11.82202 4
TGTCC 872630 0.8772246 6.6423273 1
TCCCC 785035 0.87519854 6.068128 1
ATTGG 1093030 0.865392 5.8715734 6
TGCCC 716030 0.85003525 7.553937 1
ACCTA 1381030 0.842257 8.622065 3
CGCCG 556390 0.8306122 5.006207 2
GCAGG 705810 0.82986414 6.8997846 1
GGCCT 646500 0.81726414 7.13257 2
CCGTT 807375 0.811626 6.510407 4
CTGTC 798005 0.8022067 6.249074 4
CGCCC 561665 0.78742313 5.6166553 1
CGTCC 650485 0.7722234 6.2485948 1
CCCCC 584410 0.76941437 7.5376987 1
CCGTC 641865 0.76199025 6.4901586 4
GGTGT 668050 0.76149315 5.4714317 6
GTCAA 1111910 0.7221033 5.2205286 6
GTGTG 629565 0.717625 7.24525 7
GGGCC 446925 0.71046364 8.657181 1
TGCGG 506095 0.6812619 15.335189 3
GACCG 615115 0.67918384 29.731003 2
CAGGG 558390 0.65653336 6.233608 2
CCCCG 468125 0.6562853 8.041353 2
GGCCG 402590 0.6399856 15.884625 2
GGTCA 681405 0.6371053 5.189633 9
GCCCC 447105 0.6268164 6.6617885 1
GGTAG 624525 0.6217903 6.416109 6
GTCCC 523145 0.6210518 5.5500655 2
CTAAC 1012390 0.61743236 10.636872 5
TAACT 1182625 0.61075 9.472889 6
GCCGT 482260 0.6096423 5.5145206 3
GACCC 584185 0.60574967 9.263375 2
GCCGA 547110 0.6040956 11.225456 3
TCCCG 508265 0.603387 5.7515926 2
GACTT 800265 0.59501296 7.49873 6
GGCCC 397200 0.5929639 6.707143 1
TGACT 796965 0.59255934 9.877603 1
GACTG 618745 0.5785189 6.409606 2
CCCGA 548275 0.5685141 6.6828766 3
GCGGA 481840 0.56652886 12.663614 4
GCCCG 350960 0.52393407 7.3717375 2
CCCGT 433700 0.5148671 7.393188 3
GTCCG 372605 0.47102353 5.245348 2
Produced by FastQC (version 0.10.1)