FastQCFastQC Report
Wed 6 Aug 2014
1L15C_ACAGTG_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L15C_ACAGTG_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14012045
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 148819 1.0620790898116583 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 93318 0.6659841586292365 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 90567 0.6463510501143837 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 78200 0.5580912707602638 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 58964 0.4208093822136597 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 57672 0.41158874382718585 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 55654 0.39718684888608335 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 54817 0.3912134167425241 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 54654 0.3900501318686887 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 54006 0.3854255392414169 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 43479 0.3102973191993032 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 41954 0.2994138257477763 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 41558 0.296587685808888 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 34835 0.24860753730094357 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 34402 0.2455173388324117 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 33700 0.2405073634862006 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 32749 0.23372034560265828 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 31590 0.22544889057949782 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 31445 0.2244140666119756 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 31358 0.22379317223146228 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 30883 0.2204032316481998 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 30449 0.21730589646265053 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 28558 0.2038103645827572 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 28487 0.20330365767452216 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 25290 0.1804875733699114 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 24650 0.1759200744787788 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 23832 0.17008223995854996 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 23269 0.16606426827775675 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 22794 0.16267432769449428 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 22747 0.1623389019946767 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 22663 0.16173941776521555 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 22523 0.16074027738278032 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 21834 0.15582307935779538 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 21724 0.15503804048588196 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 21582 0.1540246266694119 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 21491 0.153375185420829 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 20631 0.14723760878586958 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 19263 0.13747457990607367 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 18996 0.13556907646242927 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 18968 0.13536924838594225 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 18495 0.13199358123671456 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 18409 0.13137982357321862 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 18344 0.13091593696708798 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 18318 0.1307303823246357 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 17373 0.12398618474319774 No Hit
GCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGA 17223 0.12291567719058853 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 17145 0.12235901326323173 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 17053 0.12170243529763143 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 16702 0.11919744762452589 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 16557 0.11816262365700367 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 15914 0.1135737146148189 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 15697 0.11202504702204424 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 15606 0.11137560577346134 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 14672 0.1047099120792147 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 14619 0.10433166607729277 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 14505 0.1035180803373098 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGCT 4274625 3.4721532 7.230998 5
ACTTC 4318725 3.2279623 5.718969 60-64
AAAAA 4676750 3.0788906 4.305647 45-49
GGAAG 3559965 3.0080118 5.8933825 85-89
AGCAC 2670485 2.9828312 6.5525203 90-94
GAAGA 3777565 2.9370937 5.856274 90-94
CTACT 3795480 2.8368714 8.925464 2
AGCTG 3402020 2.8184102 6.7272596 3
CTTCT 4140335 2.6390011 5.287265 40-44
CGGAA 2682455 2.6059654 6.8176436 95-97
TCTCT 4082675 2.6022494 7.2476583 20-24
CTGGT 3663605 2.5882554 11.371582 1
GCTGC 2465905 2.5525484 6.893683 3
CTTCA 3390260 2.533996 5.722961 60-64
TTCAA 4063090 2.430519 6.6264586 9
CTGAA 3172035 2.4181173 5.0731826 80-84
TGGTG 3888245 2.389185 8.0958395 6
CGGTG 2645920 2.382166 10.718638 9
CACAC 1852165 2.3785956 10.483402 95-97
GCAGC 1954740 2.3727694 5.667559 1
TCTGC 2918285 2.3704379 5.4913664 85-89
GGTGT 3852085 2.366966 8.996314 7
CAACT 2686245 2.3544388 7.3658757 9
GGTGG 2990345 2.341608 9.674094 7
AGAGC 2383985 2.3160062 6.653715 95-97
ACAAC 2242945 2.3053098 5.2295103 85-89
CCGTG 2217540 2.295457 5.745802 60-64
TCGGA 2757695 2.2846177 5.588705 85-89
ATCGG 2741585 2.2712715 5.7994103 95-97
CAAGC 2031280 2.2688632 6.2082725 2
CAGCA 2030585 2.2680871 5.6223726 2
CAACA 2199510 2.2606668 5.080064 85-89
AAGAG 2875700 2.235885 5.211359 90-94
TATGC 3389820 2.2036734 5.832538 30-34
ATGCA 2876885 2.1931176 6.1980705 1
ATGGT 3876030 2.1915696 5.098737 1
CATGG 2631730 2.1802619 8.63423 2
GAAGC 2242180 2.1782448 5.0639586 15-19
GAGCA 2230395 2.1667957 6.1914215 90-94
ACCCA 1680055 2.1575677 23.441597 1
CGTGA 2589315 2.145123 6.740969 9
ACTGC 2232205 2.1261985 6.968618 4
GATCG 2557820 2.119031 6.463562 95-97
CTGCA 2206380 2.1016 5.1220145 95-97
GCACA 1881315 2.1013582 6.776852 95-97
GCTGG 2312080 2.081604 7.858554 4
TCAAC 2336350 2.047763 5.9968796 8
CCAAG 1826910 2.0405898 5.197065 75-79
TCCTT 3175410 2.0239692 5.40593 40-44
AGATC 2560820 1.9521737 5.9756002 95-97
CCAGC 1397335 1.9501566 6.5006995 1
CTCCT 2072105 1.9351516 5.2976375 70-74
CTCTG 2372905 1.9274416 5.530972 45-49
GCTAC 1993060 1.8984103 10.96386 1
CTTGG 2671925 1.8876553 5.5755067 2
TGCTG 2657710 1.877613 5.422773 6
GGCTC 1803930 1.8673141 5.1092453 10-14
CCTTA 2470010 1.8461698 5.6693444 45-49
TGGCT 2593290 1.8321016 7.4492307 9
TATTC 3541790 1.8067436 5.9565744 2
CATCC 1633755 1.7892007 5.211733 90-94
GCTTC 2202175 1.7887625 6.2915597 15-19
TTCGG 2526780 1.7851136 8.312941 7
GCATG 2140285 1.7731234 5.3814864 3
TTTCA 3444340 1.7570324 5.8365464 8
GTGGC 1950435 1.7560092 8.785184 8
GCAGG 1660905 1.7535129 5.0329876 90-94
CCTGT 2138905 1.7373703 7.5118403 1
CACCC 1075950 1.7264873 5.8099165 50-54
TCGGT 2420300 1.709888 9.247916 8
AATGA 2786945 1.7003467 5.2060103 75-79
ACACA 1640760 1.686381 5.4747643 85-89
ACCAG 1500945 1.6764991 5.119862 2
CACAA 1627140 1.6723822 5.2220793 4
GCACC 1197370 1.6710801 5.256033 50-54
GCTCT 2050935 1.6659147 5.034816 10-14
CCGCC 944165 1.6464447 6.5161085 45-49
TTGGT 3381280 1.6303486 10.952332 7
GTCTG 2284410 1.6138848 5.861822 90-94
TGTAG 2820150 1.5945581 5.836815 3
CTTTC 2494000 1.5896465 6.9169292 7
CCACA 1228965 1.5782672 6.140563 1
CGCAG 1291870 1.5681418 5.8930936 90-94
CCCAA 1206185 1.5490125 23.274122 2
GTGTA 2733855 1.5457658 8.070923 2
CATGC 1616910 1.5401237 5.1418347 2
TGGTA 2704660 1.5292584 8.454404 2
GCTTG 2154615 1.5221874 5.2850695 1
GCAAC 1360220 1.5193146 5.1870084 1
GTAGC 1813640 1.5025136 5.5139704 1
TACTT 2941950 1.5007522 6.2836 5
AGCCT 1566885 1.4924743 6.0131993 2
ACACG 1333520 1.4894917 6.638744 95-97
CCAAT 1646160 1.4428257 16.264618 3
ATGCC 1464100 1.3945705 5.9916196 9
GCTGT 1973125 1.3939688 5.5166173 7
GTATT 3082105 1.3674731 5.304536 4
ACATG 1788445 1.3633738 7.843768 1
CACGT 1407735 1.3408823 5.102771 95-97
ACTTT 2605825 1.3292876 6.1189365 6
GGTCA 1561550 1.2936692 14.667885 9
ACGGT 1549580 1.2837527 5.764616 4
ATGGG 1781330 1.2835397 7.0973682 3
GCGGC 967905 1.2768127 5.1420617 7
AAGCC 1124315 1.2558175 5.958642 1
GGTAC 1515325 1.2553741 9.42724 3
TGGTC 1751125 1.2371308 12.696321 8
TGTAT 2753020 1.2214642 5.1077366 3
GACGG 1145605 1.2094809 7.2669473 3
GGTCG 1340870 1.2072076 6.502565 6
TGGGT 1943700 1.1943328 5.263654 4
CAATT 1978480 1.1835164 11.202048 4
ATTCG 1782415 1.1587224 6.6456614 6
GGGTC 1214115 1.093088 6.904115 9
GTACT 1664600 1.0821326 7.34492 4
CTGAC 1126720 1.0732125 6.6767654 1
TGGGG 1355170 1.0611743 5.266192 8
ATTGG 1876200 1.0608337 10.378317 6
TGACG 1279860 1.0603026 5.6633763 2
TGGCC 993300 1.0282012 7.0446773 1
TCGTG 1388230 0.9807535 5.0683217 8
GGGGT 1252160 0.98051155 5.2764406 8
AATTG 1770575 0.9212012 8.905447 5
CGGTA 1032955 0.8557536 5.2983546 5
GCCTA 867635 0.8264314 5.804565 3
CCCCG 375100 0.6541033 5.493348 1
Produced by FastQC (version 0.10.1)