FastQCFastQC Report
Thu 31 Jul 2014
1L15B_ACAGTG_L003_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L15B_ACAGTG_L003_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13172263
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 119724 0.908909881316521 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 115756 0.8787859762593565 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 111552 0.8468704276554453 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 74741 0.5674119929126833 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 73576 0.5585676508281076 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 61611 0.46773284135003984 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 60452 0.4589340495251271 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 56573 0.42948580665296465 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 52809 0.40091061042434395 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 46126 0.3501752128696489 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 43935 0.33354177638269145 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 42662 0.3238775296241807 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 41782 0.3171968248735999 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 40172 0.30497417186401454 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 36405 0.27637620050556233 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 35819 0.271927458478471 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 35249 0.2676001838104812 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 34641 0.2629844241646253 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 34356 0.2608207868306304 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 32909 0.24983558254189123 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 30335 0.23029452114644236 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 28500 0.21636373339949255 No Hit
CGTCCCATATTTAAGTCGTCTGCAAAGGATTCATCTCCCCGATCGTCTGG 28089 0.21324354061257356 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 27630 0.20975894574835016 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 26040 0.19768812693764162 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 26004 0.19741482537966332 No Hit
GCCCAGTATTGTTATTTATTGTCACTACCTCCCCGTGTCGGGATTGGGTA 25782 0.1957294657721304 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 24648 0.18712046669581378 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 24170 0.18349162934265736 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23743 0.1802499691966369 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21738 0.165028590759234 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 20114 0.15269965381043485 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 19836 0.1505891584460468 No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT 19797 0.150293081758237 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 19417 0.1474082319795771 No Hit
GCTGCTGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 19117 0.14513071899642452 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 18101 0.13741754169348122 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 17804 0.1351628038401602 No Hit
TGACGAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 16973 0.12885409287682761 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 16807 0.12759386902614986 No Hit
GCAAAGGATTCATCTCCCCGATCGTCTGGAATTGTAATGCAAAGCGCCCA 16803 0.1275635021863745 No Hit
GCAGATGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 16723 0.12695616539086715 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 16494 0.12521766381372737 No Hit
GCAGGAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 15944 0.12104222334461436 No Hit
GCAGTAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 15884 0.12058672074798385 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 15807 0.12000215908230802 No Hit
TGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 15635 0.11869638497196723 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 15537 0.11795239739747074 No Hit
TTGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 15039 0.11417172584543749 No Hit
AGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15018 0.11401229993661681 No Hit
TGGCTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 14897 0.11309370303341196 No Hit
GGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 14177 0.10762767187384582 No Hit
TAGATGAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 13651 0.10363443244338501 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 13630 0.10347500653456433 No Hit
GACCACACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 13495 0.10245012569214569 No Hit
CTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGA 13308 0.10103047593264726 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AGCAG 5305735 4.747927 53.063625 20-24
TTTTT 7644760 4.5056214 8.869095 7
TAGCA 5863185 4.151176 33.389626 20-24
GCAGA 4449320 3.9815495 33.5872 25-29
ACTTG 5066610 3.9433124 83.38835 8
CACGG 3438535 3.8848689 37.253914 45-49
GTTTC 4293810 3.673608 32.27593 40-44
AGAGA 4810455 3.409574 21.275797 90-94
GAAGT 4326090 3.3706663 31.567438 7
TCACG 3747315 3.3496714 29.475506 45-49
CCTGC 2859065 3.2266402 37.907257 15-19
GAACC 3913640 3.182414 26.6381 70-74
CGGAT 3229160 3.1765394 29.5926 45-49
AGTAG 3881175 3.0240114 31.426752 9
AGAGC 3365875 3.01201 47.91439 95-97
CTGCG 2406110 2.9883032 40.903496 15-19
ACGGA 3314145 2.9657187 28.883514 45-49
CTTGA 3765230 2.9304564 26.08553 55-59
AACTT 5211405 2.919251 60.72654 7
TTTCA 4656835 2.867568 20.759369 40-44
CCATG 3128160 2.7962174 31.631739 75-79
GCAGC 2463620 2.7834067 18.68255 20-24
CTTGT 3234390 2.7672117 91.71406 9
AGAAG 3891570 2.758283 19.759222 6
TTCTC 3546415 2.7571278 24.991951 30-34
AACCA 4512190 2.640803 18.038406 70-74
ATAGC 3727470 2.6390748 22.941175 80-84
ACCAT 4101260 2.6385913 27.294151 75-79
AAGTT 4174625 2.5734563 21.168676 60-64
AGGAG 2599515 2.5599616 20.89894 90-94
GCGTT 2358905 2.550829 32.766895 15-19
TTCAC 3576675 2.5295346 18.753853 45-49
ACCTG 2804240 2.50667 22.557755 15-19
TTGAA 4048385 2.4956353 19.223917 55-59
CATGC 2783575 2.4881978 29.340647 75-79
TGAAG 3168965 2.469094 19.363445 60-64
TGCAT 3165455 2.4636552 24.93723 75-79
GCTGC 1980045 2.4591453 33.36186 1
TGCGT 2271515 2.4563289 35.270313 15-19
CACTG 2719480 2.4309042 28.499733 85-89
GAAGA 3428540 2.4300947 10.263253 50-54
GAGAG 2463090 2.4256124 37.976345 95-97
AGCAC 2962555 2.4090302 24.271399 80-84
GGATT 2808910 2.4058263 21.057402 50-54
GAGCC 2113830 2.3882124 52.239693 95-97
AAGGA 3365330 2.3852923 17.601194 70-74
CTCAG 2668275 2.3851328 29.430767 35-39
GCACT 2632070 2.3527699 21.228941 85-89
ACAGG 2619530 2.344131 13.571243 60-64
AAGAG 3289215 2.3313432 11.18192 50-54
TAGGG 2150280 2.3277824 23.709646 8
GTAGG 2146615 2.3238149 23.661076 7
AAACT 4550150 2.3186548 30.978157 6
GGAAC 2577495 2.306515 29.36438 70-74
TTAGC 2957830 2.3020618 25.912466 20-24
GATGA 2947840 2.296805 11.01763 15-19
TTGTA 3330340 2.2568054 29.807821 9
AGTTT 3309370 2.242595 22.762955 40-44
GACTC 2505780 2.239881 29.106262 25-29
ATGCA 3145540 2.2270644 23.443672 75-79
AGGAA 3141265 2.2264786 23.421026 70-74
AGGGA 2233455 2.1994715 21.80336 9
GTAGT 2509235 2.1491551 20.98438 40-44
ACCAG 2628710 2.1375606 13.126824 55-59
TTTCC 2745800 2.134697 11.362734 40-44
CATTC 2999650 2.1214442 23.595203 30-34
GTTAC 2722630 2.119007 23.456379 60-64
CAGAA 3283920 2.1150656 14.520631 6
CAGTA 2981070 2.1106184 23.237335 35-39
CAGCA 2595115 2.1102424 16.265919 20-24
CAGAC 2580090 2.0980248 26.56989 25-29
TCTCA 2944820 2.0826669 17.597351 35-39
CTGCA 2325730 2.0789368 18.621971 3
GTTGT 2200200 2.071547 14.118192 35-39
GTTAG 2413960 2.0675523 28.603905 20-24
TCAGT 2616015 2.036029 25.79629 35-39
CTGAA 2875320 2.0357466 22.571173 85-89
CCAGA 2498760 2.0318906 16.844233 55-59
TGTAA 3286695 2.02609 20.79388 10-14
CAGGC 1788810 2.0210037 14.888614 65-69
GGAGG 1470050 2.0114088 16.916065 90-94
GGCTC 1616650 2.0078216 18.7448 65-69
GCATA 2834600 2.0069165 17.465763 80-84
CGTTA 2576840 2.0055394 19.038351 20-24
GGAAG 2031880 2.0009634 10.147692 6
TAAGG 2564925 1.9984573 23.748837 65-69
CATAG 2820845 1.9971777 23.157417 80-84
TAGAG 2557810 1.9929136 24.441774 90-94
TTGTT 2641515 1.9677271 11.971226 35-39
ATCGG 1994345 1.9618464 5.638285 95-97
CTCAT 2773675 1.9616278 17.475233 30-34
TGTTG 2071990 1.9508339 12.872941 35-39
GGAGC 1565815 1.9468211 20.980598 95-97
TGAAT 3130695 1.9299233 18.871058 85-89
GATCG 1947675 1.9159367 5.988083 95-97
GTAAC 2668930 1.8896208 23.344128 10-14
TCATT 3059365 1.8838837 20.442684 30-34
CTAAG 2654490 1.8793973 22.981226 65-69
TAACC 2912265 1.8736383 21.016375 10-14
GGATG 1726710 1.8692473 18.395006 10-14
GAATA 3311400 1.8569694 13.685452 90-94
TAGTT 2727285 1.848145 22.23454 40-44
AACCT 2836110 1.824643 19.792276 10-14
CCTCC 1764475 1.8095031 5.3600717 1
ATTCT 2937610 1.8089103 20.543371 30-34
AGTTA 2912980 1.7957125 19.987827 60-64
CCATC 2208425 1.7938329 11.674139 75-79
TTAAA 4036785 1.7910503 14.932204 50-54
ATGAT 2902075 1.78899 9.661564 15-19
CAGAG 1993435 1.7838589 13.397837 25-29
AGACT 2514975 1.7806199 23.360308 25-29
ACTGA 2508305 1.7758973 22.757038 85-89
AATAG 3149640 1.7662573 18.309063 90-94
TTCCG 1796785 1.7655678 14.355628 40-44
GATGT 2056980 1.7617996 16.466227 30-34
AGGCT 1785610 1.7565126 14.430906 65-69
GAGCG 1395740 1.7353622 7.8652625 90-94
TGTTT 2325240 1.7321261 14.651683 35-39
TGATG 2019905 1.7300448 13.255195 30-34
CCAAA 2947735 1.7251905 23.348562 4
GGGGG 906900 1.7240698 5.8936787 1
CGGAA 1919470 1.7176702 8.296045 5
ATTAA 3863240 1.7140515 14.988067 50-54
AAGAA 3351195 1.7095686 10.797552 45-49
TTACT 2771830 1.7068268 15.197302 65-69
ACTCA 2632370 1.6935647 19.69664 25-29
TGCAG 1716680 1.6887059 21.340067 4
GAGAT 2160330 1.6832178 15.0657015 25-29
AGATC 2313795 1.638183 5.4540415 95-97
GGGAT 1509075 1.633647 20.877934 10-14
CATCA 2524630 1.624249 8.943164 80-84
GCGTC 1307575 1.6239616 5.6922283 95-97
CAGGA 1812725 1.6221477 11.925944 90-94
TCGGA 1647660 1.6208106 5.0934653 95-97
TAAAC 3178970 1.6199323 15.828683 50-54
AACAG 2501635 1.6112214 14.468796 60-64
TCCGT 1626390 1.5981331 14.777668 40-44
GATTA 2585390 1.593769 20.147821 50-54
TACTA 2842765 1.5924199 18.355827 65-69
CGTCG 1269975 1.5772638 5.7109065 95-97
AAAGA 3074535 1.5684341 8.490616 45-49
CCTTC 1724155 1.5395077 5.0743117 5
TCCTC 1719460 1.5353155 6.9291496 1
GCTCA 1697260 1.5171565 17.131298 65-69
ATAGA 2704700 1.5167437 18.137506 90-94
GAGCT 1538495 1.5134245 9.651655 95-97
GAGGA 1536355 1.5129783 17.514828 95-97
TACCA 2328395 1.4979991 9.796205 75-79
GAAAC 2309320 1.4873576 9.721912 60-64
ATGTT 2188370 1.4829491 10.311677 30-34
ACCAA 2532325 1.4820677 12.160401 3
AGCGT 1488065 1.4638164 5.9509706 90-94
AGCTG 1487580 1.4633393 7.9568753 1
TGCTG 1346665 1.4562316 6.8371024 1
AGAAA 2841995 1.4498068 7.3193407 60-64
GAGAA 2043810 1.44862 13.858097 50-54
ACTAA 2827995 1.4410831 17.032995 65-69
CTACA 2226065 1.4321638 9.80899 85-89
TACAG 2016650 1.4278022 9.643796 90-94
TAAAG 2529575 1.4185369 9.17556 45-49
GCCAA 1739570 1.414548 11.530059 3
CTCAC 1707715 1.3871223 12.153482 65-69
GTAAA 2467850 1.3839227 8.222141 45-49
AGATG 1773620 1.3819132 11.701965 25-29
CAATA 2701030 1.3763846 9.137829 80-84
ATACC 2121455 1.3648617 9.053095 75-79
AAGTA 2424615 1.3596774 23.658344 8
GACCA 1649315 1.3411564 14.55782 2
CAAAC 2289990 1.3402389 27.708586 5
CTTTT 1974625 1.3366376 5.5584717 6
TGACC 1485120 1.3275275 34.485584 1
AGCCA 1606755 1.3065482 11.894871 95-97
AAACA 2818230 1.3064134 7.4488564 60-64
AGAAC 2016735 1.2989131 10.68922 50-54
GCCCA 1256895 1.2903849 6.4470263 2
CCGTA 1442080 1.2890548 17.06721 40-44
CTGCT 1311220 1.2884389 5.0994215 2
GCCTT 1302200 1.2795756 5.468954 4
TTCCC 1415240 1.2636759 7.258388 1
TCCCA 1528155 1.2412714 6.967117 3
TGATA 1996770 1.2309129 9.637947 15-19
GATAG 1579085 1.2303416 14.87819 15-19
GATAC 1727965 1.2234111 8.726654 75-79
TCTAC 1729850 1.223403 12.072335 85-89
ATCAA 2399170 1.2225635 7.7776246 80-84
CCTCT 1362280 1.2163875 5.5443707 2
TGCCA 1355765 1.2118989 5.1066146 1
TCAAT 2145085 1.2016033 7.8227777 80-84
CCCAA 1624560 1.200408 8.074869 4
GCTGG 873015 1.1932007 6.1032133 1
CAACA 2032910 1.1897804 5.5403037 8
GGCTG 870085 1.1891961 8.390261 1
CCTTT 1519850 1.1815934 5.6507373 4
CGTAA 1620890 1.1476014 10.528562 40-44
AATAT 2576165 1.1429989 6.149023 80-84
TCCCT 1270405 1.1343518 5.3286433 2
TCCCC 1097220 1.1252202 6.5305066 2
GGCCA 994025 1.123053 12.828688 2
GGATA 1412000 1.1001576 11.072554 70-74
CCAAC 1445720 1.0682609 6.079942 5
CCGTT 1077860 1.0591333 5.866982 4
TATCT 1671120 1.0290359 8.1973295 85-89
CCCCC 872060 1.0271372 6.0081415 1
ATCTA 1810605 1.0142391 7.7866335 85-89
CCCCG 781320 1.01273 5.775203 2
GGTGC 736815 1.0070484 8.781762 2
AAGTC 1419705 1.005161 5.206023 6
TATTT 1866225 1.0005727 6.4598966 8
CCATA 1549780 0.9970683 6.374501 5
CCGAA 1219480 0.99163187 23.51085 4
ATTTA 2027635 0.98893595 5.4804754 9
CCCAG 949440 0.9747378 6.319022 2
TGGCC 781600 0.9707194 23.61126 1
TCAAC 1489400 0.95822215 6.1871195 7
CGCGG 588355 0.9235694 9.680303 7
CTGCC 789590 0.89110357 6.520374 2
CAACT 1384250 0.8905727 5.3220134 6
CCCAT 1094870 0.8893279 8.924571 4
GCGGT 650485 0.889056 8.515258 8
GTGCA 903585 0.88886076 7.111343 3
ATATC 1583255 0.88688534 6.769197 85-89
CTAAA 1704870 0.8687637 5.1640186 4
CGAAC 1050525 0.85424453 21.857134 5
GTTGC 784690 0.8485335 5.907966 1
GGGTG 560925 0.843683 10.5417385 1
GAACT 1190605 0.84295666 21.224848 6
CCCGA 820530 0.842393 5.7855997 3
GGGAG 615380 0.8419991 5.8484674 95-97
TGTCC 854790 0.8399388 8.94596 1
GACCT 937115 0.8376737 7.9499154 2
GGCCT 674110 0.83722067 6.7055745 2
GCTGT 772660 0.83552474 8.343867 1
GCAGG 669485 0.8323893 5.525496 1
CCAGT 919415 0.8218519 5.2087398 3
CCTAA 1261205 0.8114103 8.914202 4
TGTCA 1027765 0.7999035 6.2333293 5
ACCTA 1230495 0.7916526 5.800813 3
TGCCC 693015 0.78211236 7.4148 1
GTCCC 687180 0.77552724 8.291042 2
CCCCA 818470 0.76355505 6.5467772 3
CTGTC 774870 0.7614074 7.180712 4
CGTCC 663190 0.74845296 8.963246 1
GTCAA 1046125 0.74066377 6.492763 6
AGTCT 943815 0.7345657 7.070254 7
ATATT 1504575 0.73382455 5.5553136 7
TGGCT 669945 0.7244528 6.048045 1
TGCGG 515130 0.70405835 11.898235 3
ACCGA 863840 0.70243967 13.600788 3
GCCCG 488390 0.69664925 5.87801 2
GGGCC 442720 0.69495904 7.89767 1
TAACT 1239355 0.6942443 8.178645 6
CGGTA 691755 0.68048257 6.4116435 9
GGCCG 430815 0.6762712 13.096446 2
GCCCC 507095 0.6572856 6.476643 1
CCCGT 565465 0.6381639 6.130006 3
TCTAA 1132540 0.6344102 5.50868 9
TCCCG 561435 0.6336158 7.038871 2
GCGGA 494700 0.6150742 10.029161 4
GGCCC 417965 0.5961937 5.9121842 1
GCCGA 522465 0.5902828 10.173079 3
GACTT 743435 0.5786112 7.5442724 6
TGACT 726660 0.5655552 10.245089 1
GACCG 488895 0.5523553 18.085682 2
CTAAC 856010 0.5507236 8.038269 5
GACTG 552220 0.54322135 7.0063367 2
GTCTA 667825 0.5197643 7.1075478 8
GACCC 496180 0.50940067 6.016693 2
GTCCG 387980 0.48185736 6.021701 2
Produced by FastQC (version 0.10.1)