FastQCFastQC Report
Thu 31 Jul 2014
1L15B_ACAGTG_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L15B_ACAGTG_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13172263
Filtered Sequences 0
Sequence length 101
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 158895 1.2062847515267499 No Hit
GCGAGTCGGGCTGTTTGGGAATGCAGCCCTAAGTGGGAGGTAAATTCCTT 137791 1.0460693048719116 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 108083 0.820534785860258 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 102563 0.7786285469702511 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 96880 0.7354848593593979 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 95828 0.7274983804984763 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 76018 0.5771066065109692 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 64578 0.4902574447534186 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 59271 0.4499682400814499 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 59101 0.4486776493909968 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 58074 0.44088096327867127 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 54213 0.41156937118549786 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 52543 0.39889121557928203 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 45427 0.3448686076189035 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 42061 0.3193149119479318 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 41425 0.3144865844236484 No Hit
GATACCGTCCTAGTCTCAACCATAAACGATGCCGACTAGGGATTGGCGGA 39299 0.2983466090830406 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 38957 0.2957502442822467 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 36152 0.27445549788977036 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 35753 0.2714264056221774 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 35677 0.2708494356664455 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 34183 0.25950742101034574 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 31759 0.24110511610647314 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 30418 0.2309246330717812 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 30289 0.2299453024890256 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 30083 0.22838141024059422 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 27998 0.21255269500768398 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 27508 0.20883275713520144 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 27466 0.2085139053175601 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 26008 0.19744519221943868 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 25885 0.19651141189634616 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 25762 0.19557763157325359 No Hit
GATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTAAGCGGAGGAG 25553 0.19399096419499065 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 25096 0.1905215527506549 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 24116 0.18308167700568992 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 23896 0.1814115008180447 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 23836 0.1809559982214142 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 23120 0.17552033390162342 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 22630 0.17180039602914093 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 21090 0.1601091627156245 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 20392 0.1548101491748229 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 20381 0.15472664036544062 No Hit
GTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTGCT 20303 0.15413448698982096 No Hit
AAGTGGGAGGTAAATTCCTTCTAAGGCTAAATATTGGCAAGAGACCGATA 20139 0.1528894465590309 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 20090 0.15251745277178264 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 19432 0.14752210762873472 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 18856 0.1431492827010818 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 18504 0.1404770008008495 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 18335 0.1391940018203402 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 18037 0.13693167225707534 No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGT 17509 0.13292324940672684 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 17467 0.1326043975890855 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 16810 0.1276166441559814 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 16640 0.1263260534655283 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 16582 0.12588573428878547 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 16429 0.12472420266737766 No Hit
CGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTC 16361 0.12420796639119641 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 16211 0.12306920989962013 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 15526 0.11786888858808847 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 15422 0.11707935075392893 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 14574 0.11064158072155103 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 14553 0.11048215481273035 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 14479 0.10992036827688606 No Hit
CAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATTG 14414 0.10942690713053635 No Hit
GGTCCTTACGAGTTAATCGTTCTTCACTTCTTACTTGGTGTAGCTTGCTA 14225 0.10799207395115022 No Hit
TGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATT 13866 0.105266650081311 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 13652 0.10364202415332885 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 13365 0.10146320339944626 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
ACTTC 3958740 3.3250763 5.6972227 60-64
CTGCT 3673055 3.2012532 12.095595 5
AACTT 4501245 3.0620365 4.904714 55-59
CTTCT 3897035 2.8494523 7.8778143 40-44
CTACT 3287400 2.761196 12.456906 2
CAACT 2795870 2.6976104 7.041578 9
TTCAA 3776970 2.569338 7.2813897 9
TCTCT 3497545 2.557351 7.6372666 20-24
AGCAC 2193830 2.5230787 5.1286573 90-94
CTTCA 2979440 2.50253 5.5766654 60-64
CTGGT 3383700 2.4783666 11.466716 1
TCAAC 2567245 2.4770205 6.349861 8
GCAGC 2040610 2.4352064 7.392922 1
AGCTG 2849550 2.3975496 7.497724 3
ACTGC 2264510 2.2671719 12.063533 4
CGGAA 2334865 2.2566833 5.447697 95-97
GCTGC 2139500 2.222647 6.490965 3
GGTTC 3034475 2.2225792 8.279784 3
TCTGC 2504115 2.1824632 5.1317544 85-89
TGGTG 3535100 2.175988 7.955995 6
ACCCA 1580235 2.1625614 25.9783 1
GGTGG 2947120 2.1623108 8.192672 7
CAGCA 1869685 2.150286 5.316086 2
TCCTT 2918805 2.134185 7.3169713 45-49
CGGTG 2434410 2.1253622 12.157882 9
TATGC 2989430 2.1101534 5.91739 30-34
CATGG 2506945 2.109289 9.506511 2
CTGCA 2106650 2.1091263 5.529168 35-39
ATGGT 3535875 2.0975082 9.161833 1
GCTCC 1692315 2.091984 7.8104825 70-74
GGTGT 3380375 2.080749 9.723231 8
ACAAC 1874295 2.0773888 5.0300174 85-89
TGCAG 2432690 2.0468125 5.7856536 65-69
CTCCT 1964070 2.0368943 9.314652 70-74
CCAAG 1770175 2.035842 5.170595 75-79
TTTCA 3421820 2.0263634 6.7416677 8
ATGCA 2497265 2.0249202 5.1376243 1
CAACA 1821435 2.018801 5.0027122 85-89
CTCTG 2297355 2.0022612 6.84519 45-49
CAAGG 2069635 2.0003347 5.071014 4
CACAC 1450435 1.9849293 8.44697 95-97
TGGTT 3794870 1.9596844 6.0725317 2
CAAGC 1700065 1.9552096 5.4242473 2
AGCTC 1951105 1.9533986 5.867633 70-74
CAGCT 1948410 1.9507004 6.326108 65-69
CCGTG 1856965 1.9291319 5.612003 95-97
TTCCT 2628775 1.92212 6.0510683 45-49
CTTTC 2565320 1.8757226 7.665519 7
GCTAC 1867175 1.8693699 14.92738 1
CTGTT 2986840 1.8353549 7.6784277 8
CGTGA 2164995 1.8215798 7.746506 9
GATCG 2159005 1.8165399 5.179045 95-97
GCTTC 2071040 1.8050163 6.3255134 15-19
CCAGC 1265990 1.7977322 5.717127 1
CTCTA 2133530 1.7920221 6.6797857 25-29
GCACC 1249840 1.7747988 5.3497453 50-54
GCTGG 2024105 1.7671455 9.106244 4
GCACA 1532570 1.762577 5.4523273 95-97
TGCTG 2398480 1.7567494 9.018968 6
GGAGA 2159235 1.7538396 5.5931945 8
CCTTA 2085565 1.7517347 5.7651353 45-49
CTTGG 2387235 1.748513 6.4917917 2
AGATC 2150305 1.7435862 5.2784467 95-97
ATCCG 1738045 1.7400881 6.529678 95-97
CACCC 1028445 1.73778 5.8753295 50-54
CCTGT 1980795 1.7263631 8.175973 1
GAACA 1852660 1.7256657 7.5630746 80-84
CTACC 1437235 1.7122097 6.5426226 20-24
CCTAC 1427905 1.7010945 5.5037436 20-24
CCCAA 1238790 1.6952919 27.087543 2
CCAAT 1746245 1.6848739 18.881212 3
TATTC 2838635 1.6810076 6.66158 5
TGGCT 2292940 1.6794473 5.8715777 9
CATCC 1402065 1.6703107 5.001878 90-94
TCCGT 1899320 1.6553534 5.5518446 95-97
AATGA 2502290 1.6432893 5.211719 75-79
GGAGG 1935480 1.6312729 5.4593387 6
CCCCC 779015 1.6252733 5.0974693 2
TACTG 2299515 1.6231623 8.6561985 3
TTCGG 2210365 1.6189659 9.967267 7
CCTCC 1096770 1.6132869 6.163168 2
TGGTA 2716045 1.6111789 9.055718 2
TAGCT 2262710 1.5971826 5.6649795 2
ATGAT 2740015 1.5664326 5.21162 15-19
TTCAT 2619625 1.5513123 5.478784 60-64
CTGTA 2189175 1.5452764 6.4971957 8
TACTT 2592205 1.5350745 6.8249116 5
TCGGT 2085830 1.5277511 11.477944 8
GCTGT 2075570 1.5202363 9.250806 7
ACACA 1371390 1.5199903 5.2887692 85-89
ATGCC 1516980 1.5187632 6.1031055 9
TGTTT 3492495 1.5130715 6.306126 9
CCGCC 861445 1.5103904 6.548925 45-49
TTGGT 2892915 1.4939115 10.800102 7
GCAAC 1298340 1.493194 5.6243315 35-39
ACTTT 2514445 1.489026 6.753149 6
CCACA 1082960 1.4820375 5.6193953 60-64
TGTAG 2498120 1.481904 5.960796 3
GTCTG 2016455 1.4769379 5.6361456 90-94
GCTTG 1996225 1.4621205 6.1779075 1
GTGGC 1620500 1.414778 6.487956 8
GGCTG 1574665 1.3747618 17.04702 9
GCGGC 1108650 1.3728347 5.783872 7
ACGGT 1624525 1.36684 5.6791663 4
AGCCT 1360315 1.361914 6.553176 2
GTGTT 2624445 1.3552728 6.2771564 9
TCCTG 1542730 1.3445673 5.101225 75-79
ACATG 1650615 1.3384097 8.882547 1
GTGTA 2250670 1.3351148 9.083613 2
GTATT 2673485 1.3305124 6.0523305 4
ACACG 1141300 1.3125857 5.2443175 95-97
GACGG 1293000 1.2967474 6.7878733 3
GTAGC 1525240 1.2833037 6.5146947 1
AGGTA 1870535 1.2746478 5.0800066 35-39
GGTAC 1514915 1.2746167 9.787336 3
CGCAG 1062260 1.2676712 5.0806246 90-94
GCCTG 1206550 1.2534399 7.1174006 6
AACAC 1126945 1.249058 5.5342727 2
CAATT 1818320 1.2369382 13.093772 4
TGGTC 1674165 1.22623 12.88069 8
CAGGA 1263775 1.2214582 5.75783 4
TCCTC 1175695 1.2192878 5.041568 70-74
CCCTA 1020880 1.2161969 5.160785 2
GAGTC 1434565 1.2070119 12.215614 3
ATTGG 2019285 1.1978554 10.931288 6
GTACT 1657030 1.1696504 8.014998 4
GGTCA 1385015 1.1653216 14.641852 9
TGTAT 2339285 1.1641912 5.9136214 3
ATGGG 1639710 1.1594162 8.500406 3
GCGAG 1146110 1.1494317 15.311379 1
TTGGC 1559855 1.1425046 5.1547613 3
TGACG 1346375 1.1328106 5.363651 2
GTTAC 1604200 1.1323591 6.1807017 9
AAGCC 978120 1.1249156 6.6714506 1
AGCCC 780035 1.107666 5.2115793 25-29
TGGGT 1782655 1.0972917 6.149076 4
CTGAC 1095620 1.096908 6.37748 1
GGTCG 1208945 1.0554699 7.7342567 6
GCCCT 839865 1.0382135 6.0118504 1
ATTCG 1461920 1.0319278 7.8206544 6
GTTCG 1402995 1.0276135 7.922263 4
TCGTG 1401985 1.0268738 6.327088 8
TGGCC 986995 1.0253525 8.478922 1
AATTG 1724840 0.9860697 10.02872 5
CTCCC 669210 0.9843702 5.3561134 3
GTAGA 1440810 0.98181814 5.0894256 4
TACCG 940035 0.94114006 5.323717 3
GGGTC 1062540 0.92765087 7.577675 5
GCCCA 650145 0.92321944 5.2738 2
TGGGG 1244240 0.9129027 5.490114 8
CCCCG 514980 0.9029257 12.187763 1
GCCAG 753320 0.8989909 5.2975187 2
CGGTA 1050985 0.8842759 5.054764 5
TCCCC 590300 0.86829805 5.1529064 4
GGGGT 1164515 0.8544082 6.790002 8
GGGCT 977070 0.85303134 13.509135 8
CGTCC 666070 0.82337373 5.278994 6
GGCCT 785870 0.8164112 7.018199 5
GGTTG 1316585 0.810408 5.623052 5
GCCTA 750095 0.75097674 6.1356206 3
CGGTT 1016095 0.74423146 5.0336933 4
GGCCC 497350 0.73283273 6.468784 1
CGAGT 869050 0.73119974 12.397987 2
CACGG 602970 0.7195675 5.7655187 4
CCCGG 460060 0.6778868 10.478915 2
GTCGT 895485 0.65589154 6.3242483 7
CGGGC 525660 0.65092164 18.359411 7
TCGGG 741660 0.6475065 13.140957 6
AGTCG 712495 0.59947777 12.424833 4
CCGTC 482420 0.5963517 5.528263 5
CCGGT 573475 0.59576184 7.24581 3
GTCGG 675375 0.5896363 13.057588 5
Produced by FastQC (version 0.10.1)