FastQCFastQC Report
Thu 31 Jul 2014
1L15A_ACAGTG_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L15A_ACAGTG_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14516202
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 185898 1.2806242293955403 No Hit
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 183858 1.266570966703274 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 126644 0.8724320590192944 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 120532 0.8303273817765833 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 95938 0.6609029000836444 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 89501 0.6165593452061359 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 82431 0.5678551455814682 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 63271 0.4358646979423406 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 63219 0.435506477520773 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 63018 0.43412181781432907 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 55831 0.38461162224113443 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 50118 0.34525559784852816 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 47516 0.3273307990616278 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 47175 0.3249816997586559 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 44559 0.3069604570121028 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 44386 0.3057686852249645 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 39137 0.2696090892094227 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 38740 0.2668742140678395 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 37595 0.2589864759390921 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 36777 0.2533513931536638 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 34728 0.23923613077304928 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 31973 0.22025733728422903 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 31952 0.2201126713447498 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 31800 0.21906556549709075 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 30243 0.2083396194128464 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 29942 0.20626607428031107 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 28927 0.19927388720548256 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 27746 0.19113815032334214 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 26243 0.1807842023691872 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 24325 0.1675713798967526 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 23745 0.16357584442542203 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 22868 0.15753431923859973 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21610 0.14886814057836892 No Hit
GGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21337 0.14698748336513917 No Hit
TGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 21238 0.14630548679330863 No Hit
AGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21213 0.14613326543678573 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 20376 0.14036729442040005 No Hit
TGGCTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 19489 0.1342568806909686 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 18525 0.12761602518344675 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 18055 0.1243782636808168 No Hit
TGACGAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 17949 0.12364804512915982 No Hit
GTCTGGAATTGTAATGCAAAGCGCCCACGCCGCCATGTTCCGGCAACGAG 17616 0.12135405666027518 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 17368 0.11964562080356832 No Hit
TTGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 16798 0.11571897387484688 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 16142 0.11119988547968677 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 15443 0.10638457635130733 No Hit
CTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCA 15279 0.10525480425251729 No Hit
GGCCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 14868 0.10242348515128132 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 16765850 8.044429 13.36396 7
AGCAG 5739515 4.851291 55.492905 20-24
TAGCA 6737405 4.3295574 34.915943 20-24
CACGG 3848250 4.2094984 44.521805 45-49
GCAGA 4801125 4.058122 36.805946 25-29
ACTTG 5822890 4.053229 97.53246 8
CCTGC 3448405 3.71141 44.584705 15-19
CTGCG 3010645 3.5672886 48.3449 15-19
GAAGT 4971725 3.5173528 29.01847 60-64
AGAGA 5272275 3.4434617 25.146597 90-94
TCACG 4114255 3.4215822 33.91077 45-49
GTTTC 4493805 3.3883507 34.23379 40-44
CGGAT 3640495 3.333145 34.849533 45-49
GAACC 4219975 3.2399178 31.07127 70-74
CTTGA 4538135 3.158929 28.887583 55-59
ACGGA 3703785 3.1306024 33.830982 45-49
AGTAG 4403525 3.1153677 27.407068 35-39
AACTT 6260840 3.0588078 68.64783 7
GCAGC 2661425 2.9112618 18.084723 20-24
AGAGC 3426300 2.8960598 55.383785 95-97
CCATG 3473475 2.8886833 34.72051 75-79
GCGTT 2898650 2.8747573 38.005333 15-19
GAGCC 2605220 2.8497806 65.56367 95-97
TGCGT 2825770 2.802478 40.05136 15-19
CTTGT 3694680 2.7858067 105.78758 9
GAGAG 2993650 2.7857435 46.506756 95-97
ACCTG 3333940 2.7726405 26.447548 15-19
TTCTC 4042375 2.7685523 27.066074 30-34
TGAAG 3896695 2.7568 22.364202 60-64
AACCA 5102340 2.7494802 20.983784 70-74
CATGC 3282340 2.729728 33.8084 75-79
ATAGC 4236390 2.7223678 26.056805 80-84
CACTG 3150405 2.6200054 33.456963 85-89
AGAAG 3998170 2.6113102 18.971687 6
AGCAC 3400930 2.6110897 29.373032 80-84
TTGAA 4849370 2.608332 21.145334 55-59
CTCAG 3133550 2.6059878 33.957863 35-39
ACCAT 4429865 2.5857313 27.99565 75-79
AGGAG 2776340 2.5835254 19.458805 95-97
TTCAC 4077940 2.5783703 20.945116 45-49
AAGTT 4790235 2.5765254 22.796272 60-64
TGCAT 3698075 2.5741758 27.001696 75-79
GGAAC 3017745 2.550731 34.41411 70-74
GCACT 2991445 2.4878075 25.497475 85-89
AAGGA 3744575 2.4456806 20.53875 70-74
GACTC 2929390 2.4362001 33.5277 25-29
GGATT 3159125 2.4209583 23.878048 50-54
AAACT 5359450 2.4172866 38.14921 6
TTTCA 4550690 2.4082882 21.058605 40-44
ATGCA 3668130 2.3571954 26.36936 75-79
AGGAA 3577910 2.3368273 26.800478 70-74
CAGAC 3019915 2.3185627 31.107037 25-29
GCTGC 1950165 2.3107345 29.129807 1
GTAGT 3011935 2.3081608 23.857374 40-44
CTGAA 3589165 2.3064513 25.832928 85-89
TTGTA 3930065 2.2897549 30.368883 9
CAGTA 3497045 2.2472537 26.047104 35-39
TTAGC 3167635 2.2049444 28.617178 20-24
ACAGG 2606495 2.2031245 12.760306 90-94
TCAGT 3139640 2.1854575 28.533663 35-39
CATTC 3432715 2.1704125 26.032 30-34
TCTCA 3426545 2.166511 19.915602 35-39
AGTTT 3710875 2.1620493 24.152542 40-44
GCATA 3344100 2.1489687 19.987606 80-84
GGAGG 1618960 2.146442 16.584146 90-94
TAGAG 3025445 2.1404157 27.966097 90-94
GTTAC 3070535 2.1373544 26.55599 60-64
GTTAG 2788300 2.1367807 31.27707 20-24
CATAG 3318800 2.132711 26.078068 80-84
GATGA 3006210 2.1268075 9.802844 15-19
CAGCA 2750115 2.1114218 14.51578 20-24
GAAGA 3206710 2.0943868 9.051512 50-54
CTCAT 3307675 2.091353 19.795448 30-34
TAAGG 2941280 2.080871 27.04117 65-69
AACCT 3551030 2.0727515 22.692019 10-14
TGTAA 3845955 2.0686247 22.623703 10-14
TAACC 3533605 2.0625803 23.574902 10-14
CTGCA 2445585 2.0338483 16.902151 3
CAGAA 3418580 2.0280793 13.703291 5
GTAAC 3140885 2.01838 26.250387 10-14
TGAAT 3741680 2.0125387 21.048733 85-89
CTAAG 3127725 2.009923 25.876842 65-69
GTTGT 2410825 2.0012338 12.203328 35-39
ACCAG 2593555 1.9912215 12.1293 55-59
ACTGA 3071870 1.9740299 26.074121 85-89
AGACT 3063390 1.9685804 26.2336 25-29
CGTTA 2816915 1.9608134 21.42027 20-24
GGAGC 1615250 1.9452024 19.03144 95-97
GAATA 3896425 1.934783 15.553646 90-94
TGTTG 2318475 1.9245737 11.128816 35-39
TCATT 3614465 1.9128251 21.780891 30-34
ATTCT 3601300 1.9058579 21.880405 30-34
GGCTC 1587865 1.8814483 17.331701 65-69
AATAG 3786060 1.8799809 20.414425 90-94
GGGGG 988260 1.8667972 7.3405156 1
CCAGA 2421360 1.8590173 14.933243 55-59
TAGTT 3180790 1.8532083 23.737782 40-44
CAGGC 1693840 1.8528465 13.955455 65-69
ACTCA 3172355 1.8517172 22.506998 25-29
AAGAG 2833220 1.8504506 9.874756 50-54
TTTCC 2685605 1.8393242 9.967095 40-44
AGTTA 3410515 1.8344146 21.63271 60-64
TGCAG 2001840 1.832834 19.421345 4
TTAAA 4799625 1.8119322 15.804898 50-54
CCAAA 3341560 1.8006549 31.697908 4
AGGGA 1928195 1.7942834 16.753803 9
GTAGG 1775500 1.7896678 18.088623 7
CCATC 2337140 1.7654788 10.54447 75-79
GGATG 1719865 1.7335889 15.309496 10-14
TCCTC 2118395 1.7333903 8.467894 1
CCTCC 1754095 1.7148092 5.7596183 1
TGATG 2234065 1.7120494 11.652167 30-34
TAAAC 3749795 1.6912796 17.63669 50-54
GGAAG 1810905 1.6851391 8.489188 6
CAGAG 1987890 1.6802522 12.358683 25-29
TTACT 3167580 1.6763273 16.398464 65-69
ATTAA 4440170 1.6762325 15.779998 50-54
AAGAA 3642430 1.6697272 9.211308 45-49
TTCCG 1821120 1.6405368 12.817664 40-44
TTGTT 2583860 1.6306843 9.870934 35-39
CAGGA 1928090 1.6297066 11.67191 90-94
GAGGA 1739645 1.618828 17.385948 95-97
GATTA 3002185 1.614786 21.84195 50-54
ATGAT 2987025 1.6066322 8.273762 15-19
AGCTG 1744235 1.5969774 7.38061 1
GATGT 2079245 1.5934049 13.789045 30-34
GAGCT 1729075 1.5830972 8.433761 95-97
ATAGA 3172450 1.5752907 20.167538 90-94
ACCAA 2923105 1.5751635 16.26345 3
TGACC 1888735 1.570749 45.608376 1
TACTA 3201820 1.5642872 19.864643 65-69
TAGGG 1548240 1.5605944 18.056843 8
CATCA 2634385 1.5377018 7.7694883 80-84
ACTAA 3387860 1.5280354 18.534937 65-69
GAGAT 2150595 1.5214843 12.8509445 25-29
AACAG 2553965 1.5151448 12.986826 60-64
GCTCA 1805840 1.5018102 14.968729 65-69
GAGAA 2290555 1.4960217 12.110536 50-54
TGTTT 2364785 1.4924252 11.660485 35-39
CAAAC 2759900 1.4872178 36.87767 5
AGGCT 1619955 1.4831897 12.99652 65-69
GGGAT 1463970 1.475652 17.968786 10-14
TGCTG 1480035 1.4678357 6.2068424 1
GAAAC 2468660 1.4645374 8.727818 60-64
AGCCA 1874045 1.4388121 13.314141 95-97
AGAAA 3130355 1.4349867 6.498121 60-64
TCCGT 1590515 1.4327987 13.174655 40-44
TACAG 2194760 1.4103857 9.211096 90-94
AAAGA 3072750 1.4085801 7.4278975 45-49
CTACA 2407330 1.4051688 8.981685 85-89
GACCA 1802545 1.3839175 20.762827 2
TCCAA 2350470 1.3719794 5.0027947 3
CTCAC 1812845 1.3694257 10.519617 65-69
TACCA 2320780 1.3546491 8.598174 75-79
GCTCC 1247640 1.3427957 5.2331715 1
TAAAG 2696525 1.3389686 7.8790703 45-49
ATGTT 2295275 1.337285 8.319046 30-34
ATACC 2286085 1.3343977 8.22208 75-79
AGATG 1885090 1.3336471 9.932103 25-29
TCTTG 1765005 1.3308223 5.49525 4
CAACA 2447975 1.3191319 6.3261228 8
GCCAA 1706640 1.3102857 15.816123 3
GGCCA 1189095 1.3007193 19.238811 2
GAGCG 1079260 1.299724 6.4876676 90-94
AAGTA 2600050 1.2910635 20.113443 8
GTAAA 2595725 1.288916 7.06989 45-49
CTCTT 1861980 1.2752377 5.8892446 3
GCTGG 974080 1.2706656 6.4453473 1
AGAAC 2066635 1.2260354 9.290134 50-54
GCCCA 1227000 1.2191399 7.090802 2
GATAC 1878620 1.2072295 7.915098 75-79
AAACA 2884190 1.2009368 6.589931 60-64
TCTAC 1885505 1.1921535 10.626376 85-89
CAATA 2631780 1.1870185 7.96929 80-84
CCTCT 1446530 1.1836325 6.735138 2
TGGCC 994220 1.178043 31.238266 1
ATCAA 2597210 1.1714263 6.535282 80-84
CCGTA 1406025 1.1693077 14.65496 40-44
TCAAT 2378805 1.1621934 7.0019894 80-84
CGGAA 1332125 1.1259707 7.149201 5
GATAG 1584685 1.1211193 12.596961 15-19
CCCAA 1599505 1.1154542 7.8109202 3
TGATA 2048975 1.1020827 8.265571 15-19
TCAAC 1856940 1.0839038 7.040782 7
GGATA 1491070 1.0548893 9.554142 70-74
CGTAA 1636400 1.0515752 8.826824 40-44
CCGAA 1360015 1.0441617 30.325552 4
GGTGC 787355 1.027087 9.127784 2
GTCCA 1205635 1.0026551 5.579136 2
TTGAC 1427100 0.9933834 5.297475 6
CTCCG 915150 0.9849472 5.401998 2
GTGCA 1075600 0.984792 7.036474 3
GAACT 1522045 0.9780889 25.610683 6
TATCT 1840065 0.97378796 7.2492104 85-89
GTTGC 975470 0.9674295 5.4782104 1
AATAT 2544965 0.9607634 5.1857395 80-84
ATCTA 1963975 0.9595233 6.965304 85-89
GGGTG 666395 0.9570319 11.023585 1
GGCTG 727730 0.94930756 8.549486 1
TCCCC 957460 0.9360161 5.1708956 1
CTAAA 2070400 0.9338178 6.2053895 4
TGTCC 1026165 0.92441 9.077285 1
CGAAC 1189510 0.91325533 28.543482 5
GGGGT 631465 0.9068678 5.3131757 1
AACAA 2170055 0.9035809 5.25968 9
GACCT 1075100 0.894097 9.409521 2
TAAGT 1654330 0.8898149 5.2270727 5
GTCTG 893190 0.8858278 5.3049564 1
GCTGT 890560 0.8832194 7.1058397 1
TGCCC 801225 0.8623333 7.72389 1
ATATC 1757280 0.85854 6.041623 85-89
TGGCT 860360 0.8532682 7.129903 1
AAGTC 1326765 0.85259914 6.3570857 6
GGCCT 715645 0.8479619 5.805776 2
CTGTC 940760 0.8474738 8.406256 4
TGTCA 1198555 0.83429664 7.0405293 5
CCTAA 1424790 0.831656 8.972885 4
CCCCC 710495 0.829845 5.032417 1
GGGGC 478805 0.821536 5.120586 1
GTCAA 1276660 0.82040083 7.4520807 6
ACCTA 1374105 0.802071 6.6626525 3
ACCGA 1024860 0.7868441 18.37746 3
CGTCC 730070 0.7857514 5.578394 1
GGGCC 501650 0.78182715 9.578373 1
CCGTC 715845 0.7704415 5.7547603 4
AGTCT 1075620 0.7487233 7.9682264 7
GGCCG 463540 0.7224323 18.009441 2
CCCCG 542810 0.69797814 6.0528297 2
TGCGG 528835 0.6898535 10.446835 3
GACCG 629875 0.68900347 25.778946 2
GCCGA 610785 0.6681214 12.916364 3
GCCCC 517165 0.66500217 6.5542836 1
TCTAA 1353460 0.6612489 5.9934134 9
GTCCC 613110 0.65987104 5.425368 1
GACTG 706340 0.646707 7.4806447 2
GGCCC 454080 0.6428125 6.8592496 1
TGACT 917085 0.63836944 11.138089 1
TCCCG 582525 0.62695336 5.170148 2
GACTT 890060 0.61955774 7.6453805 6
CCCGA 619280 0.615313 5.547245 3
CTAAC 1051985 0.6140481 8.070224 5
TAACT 1205015 0.5887244 7.0023823 6
GTCTA 821820 0.5720569 7.9732924 8
GCGGA 472995 0.56961524 8.993022 4
GACCC 568965 0.56532025 5.03862 2
GCCCG 389165 0.55091643 5.8608 2
GGACC 481815 0.5270446 5.0448704 1
GTCCG 442900 0.52478856 6.1064415 2
CCCGT 475500 0.5117658 5.85895 3
GACTA 610280 0.39217508 5.2956543 2
Produced by FastQC (version 0.10.1)