FastQCFastQC Report
Thu 31 Jul 2014
1L15A_ACAGTG_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 1L15A_ACAGTG_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14516202
Filtered Sequences 0
Sequence length 101
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 212821 1.4660928526621495 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 147084 1.013240240112393 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 129376 0.8912524088601137 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 90945 0.6265068507588969 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 86222 0.5939707920845962 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 78514 0.540871503441465 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 75950 0.5232084811164794 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 65510 0.45128884263252883 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 62166 0.4282525139840297 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 61473 0.42347853798121576 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 58680 0.4042379680304807 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 50835 0.35019490635360406 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 48029 0.33086478129747715 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 45648 0.31446241930223895 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 44909 0.3093715560034229 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 44174 0.3043082481216505 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 43399 0.29896938606944157 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 42149 0.29035831824329805 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 39625 0.2729708500887491 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 38831 0.26750109980558273 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 38335 0.264084228092169 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 35664 0.2456840983612656 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 34914 0.24051745766557947 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 34065 0.2346688203980628 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 33810 0.23291216256152952 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 32881 0.22651241695313967 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 32827 0.22614041882305028 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 30046 0.2069825151234462 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 29892 0.20592163156726534 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 28578 0.1968696770684233 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 28418 0.19576746038667692 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 28302 0.19496835329241077 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 27824 0.1916754809556935 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 27529 0.18964326894872363 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 26970 0.18579239941687226 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 26860 0.18503462544817165 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 25496 0.17563822823628386 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 24815 0.17094691848460086 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 23855 0.16433361839412264 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 23320 0.16064808136453324 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 21968 0.1513343504037764 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 21821 0.15032168882742192 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 21302 0.14674637346600716 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 21285 0.14662926294357162 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 20940 0.14425260822355598 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 18924 0.13036467803355176 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 18834 0.12974468115006943 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 18662 0.12855979821719207 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 17095 0.11776496359033857 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 16824 0.11589808408563067 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 16486 0.11356965134544147 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 16024 0.11038700067689883 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 16020 0.11035944525985515 No Hit
GGTCCTTACGAGTTAATCGTTCTTCACTTCTTACTTGGTGTAGCTTGCTA 15951 0.10988411431585203 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 15875 0.1093605613920225 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 15691 0.10809301220801419 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 15613 0.10755568157566284 No Hit
GATGAAATTAGCAGTATTATCCGTAAACAGATAGAAGACTATAGTCAAGA 14960 0.10305725974328547 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 14845 0.10226504150328025 No Hit
CAGCTCCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGT 14797 0.10193437649875636 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 5190870 3.6330838 5.6620684 45-49
ACTTC 4393105 3.3059251 5.867554 60-64
CTGCT 4178420 3.2361162 8.6775465 5
AACTT 4945410 3.0410125 5.1248946 60-64
CAACT 3128835 2.778859 7.212243 9
CTACT 3650915 2.747408 9.67076 2
AGCTG 3450300 2.6522617 7.138371 3
TTCAA 4268415 2.6247177 6.674424 55-59
CTTCA 3486040 2.623335 6.039633 60-64
CTTCT 4085925 2.60525 6.5460634 40-44
GCTGC 2709060 2.5484943 8.241091 3
TCAAC 2828060 2.5117273 6.752502 55-59
GGAAG 3262720 2.489355 5.141172 15-19
ACTGC 2656150 2.427879 8.608272 4
TCTCT 3797020 2.4210396 7.630218 20-24
AGGAA 3230795 2.395112 5.4175196 10-14
GCAGC 2154535 2.3921092 8.983757 1
ACCCA 1842220 2.3631606 34.29056 1
GAAGC 2584505 2.344765 5.885522 15-19
CTGGT 3584650 2.3347685 9.498434 1
ACAAC 2197160 2.3030744 6.315358 85-89
CAGCA 2105850 2.2717698 6.6559305 2
CAAGC 2099805 2.2652485 7.507901 1
CTGCA 2477050 2.264171 5.5471253 95-97
CAGCT 2443575 2.233573 7.174697 65-69
CTCCT 2397920 2.2083182 9.716859 70-74
CAACA 2095505 2.1965191 6.2448125 85-89
AGCAC 2023645 2.1830878 5.191041 35-39
GCTCC 1933565 2.1629114 7.4154167 70-74
GCTAC 2349845 2.1478982 12.242113 1
CAAGG 2366800 2.1472545 5.391317 10-14
TGGTG 3920110 2.147239 7.0755243 6
CGGTG 2697185 2.1338348 13.193668 9
GGTGT 3888765 2.1300697 10.792884 1
GGTGG 3182565 2.1174467 6.321015 7
GGTTC 3244860 2.113455 6.916861 3
TATGC 3301320 2.089268 6.4457335 30-34
CCAAG 1913970 2.0647714 6.2356534 75-79
ACATC 2323300 2.0634272 5.027794 90-94
CATGG 2673835 2.0553894 14.993719 2
TCCTT 3213685 2.049096 7.0621104 40-44
AGCTC 2225100 2.0338738 6.132741 65-69
TGCAG 2643535 2.0320978 5.3495216 65-69
ATGGT 3726950 1.9835594 7.67276 1
TTTCA 3799145 1.9794264 5.329331 8
CTCTG 2501705 1.9375286 6.3725314 20-24
CTTGG 2931840 1.9095777 6.4270844 2
AAGGA 2568415 1.9040643 5.1194015 10-14
TGGTT 4212725 1.8997315 5.0538836 2
GCTTC 2428360 1.8807242 7.4539633 15-19
TGCTG 2886135 1.8798091 6.3912973 6
CCCAA 1458940 1.8714974 34.899807 2
GCTGG 2359780 1.8669025 8.563462 4
CCAGC 1399945 1.8482188 7.381301 1
CTGTT 3432425 1.8405393 5.451041 8
CGTGA 2373235 1.824317 12.769315 9
CTTTC 2825055 1.8012995 6.287687 7
CACAA 1712865 1.7954339 5.8366847 85-89
CCAAT 2003485 1.7793851 24.278915 3
CCTCC 1324025 1.7611285 5.6686726 1
CCTGT 2273760 1.7609891 7.7355413 1
CACCC 1115830 1.7516822 5.598023 50-54
TAGCT 2744825 1.7370855 5.0995946 2
CTCTA 2307575 1.7365099 5.9419355 25-29
GGAGA 2273020 1.7342443 5.2471538 7
CCTTA 2284905 1.7194501 6.4128757 95-97
CATCC 1552925 1.6878732 5.8970237 90-94
TATTC 3226035 1.6808252 7.2690997 5
AATGA 2724490 1.662833 5.369467 75-79
GCAAC 1540610 1.6619946 5.610007 1
CTACC 1528205 1.6610051 5.6839204 25-29
GCAGG 1765740 1.648691 5.434684 4
GGAGG 2084150 1.6365398 5.058839 7
TACTG 2578940 1.6321038 5.9452777 3
GCTGT 2474510 1.6117079 6.50306 7
GAACA 1821105 1.6053376 5.632361 80-84
TTCGG 2463140 1.6043022 10.71625 7
ACACA 1516070 1.5891523 5.9277635 85-89
ATCCG 1727715 1.5792341 6.1577773 95-97
CTGTA 2493220 1.577855 6.4171615 8
TCGGT 2404440 1.5660695 12.949922 8
CCACA 1213840 1.5570881 5.961251 1
ATTCC 2037945 1.5336063 5.0673695 80-84
TGTAG 2865070 1.5248492 5.5522394 9
TTGGT 3379525 1.5239993 12.64352 7
TACTT 2862965 1.4916589 5.6878943 5
TTCAT 2857700 1.4889158 5.3988576 60-64
TGGTA 2789345 1.4845467 7.1652102 2
ACATG 1973900 1.4743279 14.684582 1
CCGCC 912280 1.4739271 6.8879094 45-49
GCTTG 2260595 1.4723799 6.2771387 1
GCGGC 1287255 1.4708983 7.871705 7
TCCTG 1878455 1.454832 5.062808 75-79
GCAAG 1599625 1.451243 5.218406 1
GGCTG 1818855 1.4389582 6.2913914 9
ACGGT 1870515 1.4378736 6.9807854 4
ATGCC 1569360 1.4344884 7.516171 9
AACAC 1317075 1.3805648 5.529496 85-89
GTGTA 2581960 1.3741721 10.121495 2
GTGTT 3042880 1.372189 5.208257 9
GTAGC 1764050 1.3560337 5.9099474 1
CAATT 2187915 1.3453842 16.72487 4
ACTTT 2565925 1.3368955 5.6674967 6
GGTAC 1708320 1.3131936 8.230849 3
ATGGG 1996615 1.2907406 13.014811 3
GACGG 1379520 1.2880732 8.855315 3
GTATT 2932605 1.2849675 6.5855103 4
TGGTC 1905075 1.240821 15.145706 8
GGTCA 1588725 1.2212603 17.67285 9
AGCCT 1333805 1.2191771 5.9196258 2
AAGCC 1126995 1.2157909 7.382386 1
GGTCG 1526460 1.2076344 12.959795 6
GTATG 2240490 1.1924348 5.119627 7
GCCTG 1257360 1.1828364 7.5102468 6
GTACT 1859150 1.1765786 6.62017 4
TGTAT 2676630 1.172808 6.5062647 3
CTGAC 1282585 1.172359 8.067024 1
TGGGT 2127930 1.165573 9.578901 4
TGGCC 1212050 1.1402118 10.776025 1
TGACG 1479110 1.1369989 6.9827843 2
TCGTG 1719000 1.1196259 10.665106 8
GCTCA 1213340 1.1090649 5.111848 1
ATTGG 2052480 1.0923721 13.032411 6
GGGTC 1380230 1.091947 12.925914 5
CCAGG 983140 1.091548 5.078291 1
TGCGG 1362790 1.0781497 5.7334485 6
ATTCG 1639790 1.0377549 8.749169 6
TTGGC 1573720 1.0250015 5.1529965 3
AATTG 1963600 1.0154394 12.052179 5
GTTCG 1552575 1.0112294 6.568029 4
CGGCT 1069345 1.005965 6.2138515 8
TGGGG 1486945 0.9893048 5.5583005 8
GGCCA 850190 0.94393796 5.3334165 1
CGGTA 1215450 0.9343221 6.4852185 5
GCCCA 705555 0.93147933 5.761389 2
GGGGT 1383725 0.9206297 5.527663 8
CAGGC 775800 0.8613452 5.192936 1
CTACA 951425 0.8450033 5.217662 3
GGCCT 891680 0.8388302 7.2137747 5
GGCCC 608700 0.8270585 8.024822 1
CCCCG 496230 0.8017351 8.287487 1
GTCGT 1197240 0.7797912 10.489885 7
GCCCT 692195 0.7742985 5.470578 1
GCCTA 788140 0.72040683 5.6583366 3
CCCGG 443215 0.60220927 6.8272862 2
GGTAA 906215 0.56922674 5.08684 7
Produced by FastQC (version 0.10.1)