FastQCFastQC Report
Thu 31 Jul 2014
13S15B_GGCTAC_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 13S15B_GGCTAC_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12257282
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 241324 1.968821472819178 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 114226 0.9319031739663001 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 111123 0.9065876105322535 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 90440 0.7378471018289373 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 89407 0.7294194585716475 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 75304 0.6143613241500032 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 73030 0.5958090872022035 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 68519 0.5590064746817442 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 58585 0.47796077466439946 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 57298 0.4674608938588506 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 50764 0.41415380669221774 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 49423 0.4032133714472752 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 47360 0.3863825601793285 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 45658 0.3724969369228839 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 45027 0.3673489767144135 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 44132 0.3600471948022408 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 42396 0.34588418541728905 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 36601 0.2986061673379139 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 35440 0.28913424689095024 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 34436 0.28094319768444587 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 33860 0.2762439503309135 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 33631 0.2743756731712626 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 33486 0.2731927029173352 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 33164 0.2705656931120619 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 33151 0.27045963370998566 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 32907 0.2684689803171698 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 32439 0.26465084184242477 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 30236 0.24667785239827233 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 29585 0.24136672387891542 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 27715 0.22611048681102386 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 26657 0.2174788831651258 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 25086 0.20466201234498807 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 24394 0.19901638878831376 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 24263 0.1979476363520069 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 23726 0.19356656720470328 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 23161 0.1889570624221585 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 23145 0.18882652777344927 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 23131 0.18871230995582872 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 23040 0.18796989414129495 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 22611 0.18446993387277866 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 22033 0.17975436968815764 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 21819 0.17800846876167162 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 21723 0.17722526086941623 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 20739 0.16919737997379844 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 18881 0.1540390438924388 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 18642 0.15208918257734463 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 18602 0.15176284595557155 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 18178 0.1483036777647769 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 17861 0.14571746003722522 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 17264 0.140846885957262 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 16258 0.13263951991966896 No Hit
GATGAAATTAGCAGTATTATCCGTAAACAGATAGAAGACTATAGTCAAGA 16073 0.13113021304396846 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 15624 0.12746708446456562 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 15611 0.12736102506248936 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 15335 0.12510930237225512 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 15182 0.12386106479397307 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 15088 0.12309417373280633 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 14724 0.12012451047467129 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 14545 0.11866415409223677 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 14409 0.11755460957820828 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 14288 0.11656744129734471 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 14160 0.11552316410767084 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 14079 0.11486233244858035 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 13934 0.11367936219465295 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 13803 0.11261060975834611 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 13575 0.11075049101423953 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 13102 0.10689156046177284 No Hit
CGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTC 13090 0.10679365947524093 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 12790 0.10434613481194281 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 12623 0.10298367941604021 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 12487 0.10187413490201172 No Hit
GGTCCTTACGAGTTAATCGTTCTTCACTTCTTACTTGGTGTAGCTTGCTA 12303 0.10037298644185555 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGCT 3876205 3.5362065 10.800536 5
ACTTC 4172120 3.5249612 7.044257 60-64
AACTT 4680190 3.2046046 6.036795 60-64
AAAAA 4064200 3.1880205 5.802128 45-49
GGAAG 2957595 2.9206846 6.721436 15-19
CTACT 3413690 2.8841753 12.199613 2
AGCTG 3017895 2.8644588 8.881901 3
CAACT 2839400 2.8522327 8.3180275 9
TTCAA 4109235 2.8136623 7.947377 55-59
TCAAG 3169585 2.7861693 5.849333 10-14
CTTCA 3285630 2.7759793 7.151411 60-64
AGCAC 2098100 2.705694 6.0807414 35-39
CTTCT 3804205 2.7033398 6.2277255 40-44
TCTCT 3795845 2.6973991 9.02347 20-24
CTGGT 3324355 2.6539032 11.502881 1
TCAAC 2624960 2.6368234 7.9235687 55-59
GCTGC 2250000 2.6351633 8.878905 3
AGGAA 2800300 2.561045 6.9116807 40-44
ACTGC 2288265 2.4819784 11.09253 4
ACCCA 1670160 2.4612956 42.287533 1
GGTTC 3062270 2.4446747 5.001951 3
CGGTG 2383695 2.442994 14.238391 9
ACAAC 2029975 2.424431 7.6588798 85-89
CAGCA 1864890 2.404948 6.896778 35-39
GAAGC 2099665 2.3694592 7.6507607 15-19
ACATC 2349415 2.360033 6.792868 90-94
CAAGC 1824885 2.3533578 8.218416 75-79
GGTGT 3355110 2.343857 12.012857 1
CTGAA 2666290 2.3437567 6.4183793 80-84
GCAGC 1677600 2.3360078 8.885401 1
TATGC 3146240 2.3261416 7.5687327 30-34
GGTGG 2579975 2.3138435 10.379966 7
CAACA 1930765 2.305943 7.511767 85-89
AAGCT 2615280 2.2989173 5.837045 15-19
GCTAC 2108450 2.286941 15.914134 1
TGGTG 3234035 2.2592747 8.451461 6
CAAGG 1992875 2.2489474 7.180889 10-14
CTGCA 2056165 2.2302299 5.969824 95-97
TCTGC 2422940 2.2104135 5.247542 85-89
CATGG 2318445 2.2005703 14.309852 2
CCGTG 1853005 2.1702092 6.255053 60-64
CTCTG 2372700 2.1645803 8.194469 45-49
ATGGT 3312655 2.1432223 6.4000773 1
CGTGA 2246885 2.1326485 12.677589 9
TCCTT 3000175 2.1319811 6.6408343 40-44
CGGAA 1883295 2.125287 5.8450294 95-97
CCAAG 1648005 2.1252549 7.9298873 75-79
ATGCA 2369515 2.0828815 5.762972 1
CAGCT 1902645 2.0637136 6.76348 2
GTTCC 2250785 2.0533588 5.2645864 70-74
GCTCC 1506200 2.0158622 6.3213625 70-74
TTTCA 3460295 1.9927981 5.9320793 8
CTCCT 1886925 1.9671558 8.030243 70-74
GCTGG 1915530 1.9631826 10.204979 4
CTTGG 2430710 1.9404871 7.0751815 2
AAGGA 2118445 1.9374472 6.379488 10-14
GCTTC 2123545 1.9372798 8.865931 15-19
GGTAT 2994200 1.9371883 5.6873317 30-34
CCTTA 2265345 1.9139556 8.019781 95-97
TGCTG 2386210 1.9049618 8.34613 6
CCAGC 1195850 1.9028989 7.0536118 1
CCCAA 1275370 1.8794984 43.16625 2
TGGCT 2353530 1.8788728 7.642625 9
AGCTC 1726585 1.8727493 5.4503527 65-69
TTCCA 2211980 1.8688686 5.8604946 75-79
CTCTA 2210470 1.8675928 5.464429 25-29
CCAAT 1840055 1.848371 29.503345 3
CTGTT 2971870 1.8480482 6.776669 8
CCTGT 2003660 1.8279103 9.179395 4
GATCG 1914825 1.817471 5.459552 95-97
TAGCT 2451060 1.8121669 6.3900986 2
GGCTC 1545415 1.8099648 5.7073994 10-14
CATCC 1459105 1.8085539 7.4569154 90-94
TCTGG 2261345 1.8052795 5.0719285 45-49
CACAA 1507105 1.7999592 7.1856875 85-89
CTTTC 2523165 1.7930087 7.19053 7
TTCGG 2234755 1.7840521 11.03169 7
GCAGG 1458275 1.7769369 5.7829165 90-94
TATTC 3066600 1.7660674 6.9415064 5
AATGA 2475900 1.7638096 6.3006496 75-79
CACCC 961520 1.7484394 6.2658944 50-54
TACTG 2352790 1.7395122 7.6560407 3
TCGGT 2173260 1.7349596 13.204137 8
GCCTT 1875700 1.7111744 5.3654876 35-39
GTGGC 1643025 1.6838984 9.093673 8
GCACA 1300505 1.6771215 5.032351 95-97
GCACC 1044125 1.6614664 5.747645 1
CCGCC 837315 1.644048 7.984557 45-49
ACCAG 1268095 1.6353258 5.413772 30-34
TTGGT 3004380 1.634877 16.710716 7
ACGGT 1719855 1.6324137 8.153129 4
AGATC 1851715 1.6277183 5.2681766 95-97
TGTAG 2505125 1.6207664 6.592378 6
GAACA 1539595 1.6090605 6.6899667 80-84
TGGTA 2486635 1.6088037 8.823236 2
TGAAC 1805685 1.5872564 6.096079 80-84
CTGTA 2131510 1.575911 6.958636 5
CCTCC 1030260 1.5757172 6.476355 1
CCACA 1066155 1.5711803 5.9259553 1
CTACC 1262090 1.5643548 5.7720504 20-24
GCAAC 1208865 1.5589433 6.300328 1
TTAGG 2407310 1.5574821 5.553587 35-39
ATGCC 1433540 1.5548964 8.571504 9
GCACT 1430155 1.5512251 5.5195746 35-39
GCTGT 1939325 1.5482042 8.440937 7
TACTT 2688240 1.5481687 6.41781 5
GCTGA 1624500 1.5419068 5.922452 80-84
CACAC 1038495 1.5304183 5.8129153 95-97
CGCAG 1095485 1.52543 6.5611906 90-94
TCCAA 1517895 1.5247551 6.144395 75-79
ACACA 1261670 1.5068324 6.952161 85-89
GTGTA 2325910 1.5048178 10.913628 2
ATTCC 1764880 1.4911203 5.2759995 80-84
GCTTG 1857875 1.4831809 6.8758755 1
GACGG 1204230 1.467378 10.549009 3
GGTAC 1541690 1.4633071 10.165992 3
GTCAA 1664045 1.4627502 5.487664 10-14
CATGC 1340430 1.4539043 5.0914083 2
TTCAT 2523900 1.4535246 5.2840424 60-64
ATCTC 1717790 1.4513348 6.010366 45-49
AACAC 1214645 1.4506698 6.980426 85-89
GTAGC 1526290 1.4486902 7.6583014 1
CATGA 1645555 1.446497 5.233919 65-69
GCGGC 958345 1.4409238 7.8369384 7
AACCA 1193475 1.4253861 5.328858 75-79
ACATG 1618510 1.4227234 13.49168 1
GCAAG 1238385 1.39751 5.321807 1
GTCTG 1747175 1.3948067 5.632242 90-94
AGCCT 1283580 1.3922417 7.1220827 2
GGCTG 1356355 1.390097 6.361348 9
ACTTT 2406530 1.3859307 6.3379707 6
GGTCA 1441230 1.3679546 24.904297 9
GTATT 2684515 1.3528908 6.557065 4
ATGGG 1609005 1.3364187 13.077125 3
ACCGC 838040 1.3335329 5.540525 45-49
AGGTA 1732630 1.3327776 5.4575987 6
TTCTC 1871275 1.3297634 5.556839 20-24
CAGGT 1390160 1.3194811 5.1313987 95-97
TGGTC 1651035 1.3180561 21.155947 8
CAATT 1921575 1.3157347 20.005337 4
GAATC 1488080 1.3080713 5.184251 40-44
GCCTG 1114190 1.3049213 8.082984 6
GGTCG 1262615 1.2940251 13.044608 6
CTGAC 1180210 1.280121 9.212699 1
TGCCT 1381115 1.2599714 5.2548275 30-34
GTATG 1941030 1.2558081 5.683852 7
GTACT 1687925 1.2479507 7.8069167 4
AAGCC 960940 1.239221 8.112657 1
TGGGT 1743540 1.2180251 9.498836 4
TGACG 1279585 1.2145281 7.9964914 2
TGTAT 2363785 1.1912555 6.4188004 3
GGGTC 1146330 1.1748472 12.974065 5
TGGCC 995880 1.1663584 11.451059 1
ACACG 901450 1.1625031 5.128025 95-97
CGGCT 984960 1.1535691 5.9228683 8
ATTGG 1742545 1.1273923 17.760313 6
GCGAC 807530 1.1244613 5.8267503 20-24
ATTCG 1512450 1.1182151 8.812528 6
GCTCA 997825 1.0822959 5.4826097 1
CGCCT 808390 1.08193 5.145321 45-49
TCGTG 1318515 1.0525984 10.093682 8
CGGTA 1103410 1.0473102 7.6212726 5
TGCGG 1005850 1.0308725 5.5992756 6
TGGGG 1149190 1.0306479 6.514814 8
CTTAT 1767480 1.0178992 5.4918933 95-97
AATTG 1694280 1.0151796 15.86612 5
TTGGC 1257650 1.0040085 5.5773726 3
CACGG 711515 0.99076325 5.1277356 4
GGGGT 1101100 0.98751855 6.333534 8
AACGG 838390 0.9461181 5.167332 60-64
AGTAG 1180860 0.90834385 5.1074824 9
CTACA 889280 0.8932991 5.486594 3
GGCCT 745940 0.8736327 7.7917504 5
GCCCA 534785 0.8509779 5.445259 2
GCCTA 743480 0.8064194 6.893268 3
CCCTA 628575 0.7791159 5.1230264 2
GGCCC 436620 0.75019825 7.2898064 1
GTCGT 912470 0.7284441 9.942761 7
CCCCG 366740 0.7200852 5.4435472 1
GCCCT 532755 0.7130267 6.1539207 1
CCTAT 812245 0.68625355 5.240461 4
GGTAA 741215 0.57015914 5.337164 7
Produced by FastQC (version 0.10.1)