FastQCFastQC Report
Thu 31 Jul 2014
13S15A_GGCTAC_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 13S15A_GGCTAC_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9363930
Filtered Sequences 0
Sequence length 101
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 135491 1.4469458870367462 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 134667 1.4381461629892578 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 114649 1.2243684008744193 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 86178 0.9203187123355259 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 74570 0.7963536677442058 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 63918 0.6825980117322534 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 59426 0.6346267005413325 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 55563 0.5933726544303514 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 46449 0.49604172607014363 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 45811 0.489228347499394 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 38448 0.4105968327400995 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 36620 0.3910751148289233 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 35054 0.3743513674279923 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 34844 0.3721087193090935 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33730 0.3602120050021732 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 33624 0.3590800016659672 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 33103 0.35351609847574683 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 31958 0.34128832658937003 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 31512 0.33652536915589926 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 30314 0.3237315956014195 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 29570 0.31578621369446375 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 28940 0.30905826933776737 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 27988 0.2988915978654262 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 26342 0.2813135083239623 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 25667 0.2741049965132161 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 23778 0.2539318427198836 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 22424 0.23947210199136473 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21763 0.23241310005521187 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 21404 0.22857923969957059 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 20453 0.21842324750398603 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 19949 0.21304089201862894 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 19595 0.20926042804677095 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 17868 0.19081731708801752 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 16760 0.17898467844163723 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 16044 0.171338316283868 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 16000 0.1708684281065749 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 15734 0.1680277404893031 No Hit
GGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15589 0.16647924535958727 No Hit
AGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15281 0.1631900281185357 No Hit
TGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 15184 0.16215413827313957 No Hit
TGACGAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 14787 0.1579144654007452 No Hit
GCAGGAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 14518 0.15504173995320342 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 14192 0.15156029573053195 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 13738 0.14671190408300788 No Hit
GCAGATGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 13337 0.14242951410358684 No Hit
CTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGA 13183 0.14078490548306105 No Hit
GCAGTAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 12903 0.137794707991196 No Hit
TTGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 12843 0.13715395138579636 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 12755 0.1362141750312102 No Hit
GCTGCTGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 12249 0.13081046099233976 No Hit
GCAGAGGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 12201 0.13029785570802002 No Hit
TGGCTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 11742 0.12539606767671266 No Hit
CTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCA 11708 0.1250329722669862 No Hit
GCAGCAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 11432 0.12208549188214779 No Hit
CGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 11226 0.11988556087027562 No Hit
GGCCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 10344 0.11046643877090068 No Hit
TGGTCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 10064 0.10747624127903561 No Hit
GACCACACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 9632 0.1028627937201581 No Hit
GTCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 9399 0.10037452223585609 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 8465945 6.5653768 11.595045 7
AGCAG 4752205 5.933318 70.350296 20-24
CACGG 3021810 5.440951 55.317696 45-49
TAGCA 5224665 5.040117 42.39342 20-24
GCAGA 3882000 4.8468323 44.693283 25-29
CCTGC 2368985 4.752858 62.54695 15-19
ACTTG 4188160 4.6253853 115.80233 8
TCACG 3187685 4.4346848 42.748196 45-49
CTGCG 2130225 4.3911233 63.58323 15-19
GTTTC 3433410 4.3410306 44.893578 40-44
CGGAT 2915730 4.1676598 40.94469 45-49
GAACC 3337600 4.0558243 37.055836 70-74
AGAGA 4326275 3.7455137 24.853392 90-94
GAAGT 3761085 3.727803 37.146595 7
ACGGA 2966145 3.7033508 37.843094 45-49
GCAGC 2013575 3.6255631 28.423557 20-24
AGTAG 3492025 3.461124 37.088963 9
GCGTT 2088420 3.4174747 47.181705 15-19
CTTGA 3048955 3.367252 34.369724 55-59
AACTT 4464855 3.3278925 78.27933 7
TGCGT 2020260 3.3059382 49.94641 15-19
TTCTC 2639305 3.2478735 36.13347 30-34
AGGAG 2525785 3.2400854 28.586996 90-94
ACCAT 3449280 3.2385678 38.96942 75-79
GAGCC 1791925 3.2264688 80.46833 95-97
ATAGC 3328270 3.2107072 29.271532 80-84
ACCTG 2307630 3.2103584 33.09408 15-19
AACCA 3890180 3.1904485 23.840185 70-74
CCATG 2287395 3.1822076 42.763783 75-79
CATGC 2282130 3.1748831 42.583885 75-79
CTTGT 2486620 3.1439571 132.68442 9
AGAGC 2480275 3.0967226 61.207844 95-97
ACAGG 2432375 3.0369177 18.64465 90-94
CACTG 2142830 2.9810898 41.811813 85-89
CTCAG 2137320 2.9734244 42.75745 35-39
TTCAC 2699995 2.9022179 26.32424 45-49
GCACT 2052975 2.8560843 31.570808 85-89
GCTGC 1384150 2.8532073 45.664913 1
GACTC 2050345 2.8524253 42.36084 25-29
AGAAG 3269195 2.8303368 23.934074 6
TGCAT 2538860 2.8039057 31.897085 75-79
AGCAC 2291540 2.7846608 34.41967 80-84
GAGAG 2138720 2.743557 47.756737 95-97
TTTCA 3182770 2.7158742 25.219166 40-44
TGAAG 2719575 2.6955094 23.369509 60-64
GGCTC 1303650 2.6872687 29.620287 65-69
GGAAC 2143595 2.6763642 38.279972 70-74
AAGTT 3458285 2.6483798 24.46566 60-64
GGAGG 1381170 2.625246 25.22928 90-94
CAGAC 2134855 2.5942585 37.172386 25-29
TTGAA 3347880 2.5638309 22.48556 55-59
GATGA 2567045 2.5443292 13.056079 15-19
CAGGC 1411115 2.5407975 22.457544 65-69
GTTAC 2287495 2.5262995 31.679039 60-64
GGATT 2220990 2.5201647 26.52986 50-54
TCTCA 2336160 2.5111325 25.296064 35-39
TTAGC 2273450 2.5107882 34.3366 20-24
CATTC 2311155 2.4842548 33.353 30-34
AAACT 3814355 2.4833643 40.39903 6
TCAGT 2232025 2.4650385 34.295086 35-39
ATGCA 2535835 2.446263 29.717915 75-79
TTGTA 2778740 2.436183 33.62734 9
GTTAG 2142995 2.4316633 35.32203 20-24
GTAGG 1648760 2.4213638 28.843386 7
CTCAT 2249910 2.4184227 25.22157 30-34
GTAGT 2131245 2.4183307 26.517832 40-44
GTTGT 1856845 2.4121284 18.772007 35-39
CAGCA 1968165 2.391698 22.573582 20-24
ACCAG 1962160 2.3844006 18.357916 55-59
TAACC 2532870 2.3781402 28.678028 10-14
CTGAA 2464895 2.3778288 28.902645 85-89
CCATC 1753720 2.3745973 19.904404 75-79
CAGTA 2457270 2.3704731 29.552868 35-39
AGTTT 2675700 2.3458457 27.42156 40-44
CGTTA 2112270 2.3327816 26.10972 20-24
TTTCC 1892175 2.328471 16.491652 40-44
CAGAA 2730400 2.3007314 18.432299 6
AACCT 2435340 2.2865684 27.377388 10-14
GGAGC 1229925 2.2753274 28.426924 95-97
TGTTG 1748500 2.2713833 16.727259 35-39
CATAG 2353285 2.2701612 29.408932 80-84
TAGAG 2289065 2.268809 29.463577 90-94
GCATA 2327295 2.245089 22.383326 80-84
AAGGA 2580080 2.233729 20.32934 70-74
CCAGA 1825330 2.218126 22.978424 55-59
TTCCG 1392350 2.2175748 22.260736 40-44
AGGAA 2556440 2.2132623 26.716166 70-74
TCATT 2587975 2.2083325 26.461239 30-34
GTAAC 2288040 2.207221 29.803892 10-14
CTAAG 2281525 2.2009358 29.326958 65-69
GAAGA 2537555 2.1969123 11.52865 50-54
CTGCA 1564290 2.1762292 26.797575 3
TGTAA 2840060 2.1749387 24.06976 10-14
TAGGG 1470365 2.1593735 28.921741 8
AGGGA 1663175 2.1335263 25.442066 9
TAAGG 2141665 2.122713 28.453466 65-69
ATTCT 2482110 2.1179974 26.459417 30-34
AGACT 2185155 2.1079698 29.752281 25-29
ACTGA 2168100 2.0915172 29.313745 85-89
TCCGT 1312375 2.0902 22.938704 40-44
ACTCA 2220145 2.0845191 27.166946 25-29
AAGAG 2370865 2.052599 12.880832 50-54
GGATG 1394575 2.0480685 21.507814 10-14
AGTTA 2632045 2.0156393 23.305405 60-64
TAGTT 2286475 2.0046034 27.043175 40-44
TGCAG 1400130 2.001305 30.438292 4
ATGAT 2601120 1.9919567 11.24094 15-19
TTGTT 1964100 1.9713697 15.203978 35-39
AGGCT 1376485 1.9675077 19.906864 65-69
TGAAT 2567675 1.9663444 22.272661 85-89
CAGAG 1573335 1.9643717 18.108177 25-29
GCTCA 1407915 1.9586813 24.998343 65-69
TTACT 2294320 1.9577551 19.863375 65-69
GATGT 1722950 1.955037 20.577518 30-34
TTAAA 3743100 1.9345864 16.426243 50-54
CAGGA 1549335 1.9344068 17.679667 90-94
CTCAC 1407505 1.9058102 18.712917 65-69
TGATG 1672180 1.8974283 16.837132 30-34
GAATA 2757095 1.8442878 15.542205 90-94
TGTTT 1835435 1.8422285 18.598982 35-39
GGGAT 1253860 1.8414147 26.403625 10-14
GAGGA 1429525 1.8337994 25.079903 95-97
AATAG 2721950 1.8207786 20.528997 90-94
CCAAA 2179275 1.787286 34.714634 4
CTACA 1902030 1.7858374 14.223905 85-89
CATCA 1895455 1.779664 12.104771 80-84
TACTA 2369725 1.7662809 22.899845 65-69
TACAG 1825445 1.7609657 13.290062 90-94
TAAAC 2673960 1.7409017 19.102676 50-54
AGCTG 1217580 1.7403735 11.3527355 95-97
TACCA 1847185 1.7343428 13.713624 75-79
ATTAA 3355240 1.7341245 16.350292 50-54
ATACC 1836490 1.7243012 13.134808 75-79
TGACC 1236970 1.7208639 56.88432 1
CCGTA 1229890 1.7110143 24.881407 40-44
GAGAT 1724130 1.7088732 18.061132 25-29
TCTAC 1569035 1.6865518 18.953608 85-89
GATTA 2201780 1.6861392 23.494108 50-54
TCCTC 1067805 1.6552521 10.67828 1
AAGAA 2827450 1.6520736 11.468515 45-49
AACAG 1950235 1.6433369 17.51308 60-64
GGAAG 1279815 1.6417509 11.483035 6
CCTCC 823190 1.6074407 6.0642624 1
GAGCT 1115805 1.5948993 14.042618 95-97
ACTAA 2439645 1.5883491 20.19765 65-69
ACCAA 1930290 1.5830863 18.09512 3
ATAGA 2361810 1.579872 20.407385 90-94
GAAAC 1865860 1.5722395 12.002451 60-64
ATGTT 1765260 1.5476427 12.404591 30-34
GAGAA 1780620 1.5415887 16.054958 50-54
TAAAG 2270250 1.5186254 10.480083 45-49
CTCCT 976825 1.5142201 5.037605 3
GTAAA 2258505 1.510769 9.6675005 45-49
GATAC 1541535 1.4870844 11.86716 75-79
AGATG 1494185 1.4809628 13.976895 25-29
TCTCC 954820 1.480109 5.0894084 1
AAAGA 2504715 1.4635003 9.144435 45-49
TGCTG 894305 1.4634339 8.572618 1
GGGGG 513325 1.4456997 7.4849954 1
GAGCG 781265 1.4453187 6.6837645 90-94
CAAAC 1756935 1.4409128 40.69109 5
AGCCA 1184570 1.4394797 15.719376 95-97
GACCA 1162420 1.4125632 23.757942 2
AGAAA 2400360 1.4025258 8.05617 60-64
TCAAT 1865400 1.3903811 10.82036 80-84
AGAAC 1647530 1.3882669 12.830782 50-54
GGCCA 752095 1.3541923 21.966522 2
CTCTT 1094450 1.3468072 7.552779 3
CGTAA 1387985 1.3389579 13.387639 40-44
CAATA 2041200 1.3289386 11.805537 80-84
GATAG 1337185 1.3253522 17.914507 15-19
TATCT 1552675 1.3249056 11.847699 85-89
GCTCC 656935 1.3179988 6.203424 1
CTGCT 821120 1.3077854 5.462481 2
AAGTA 1938880 1.2969639 25.8248 8
AAACA 2255125 1.2824706 8.736913 60-64
ATCTA 1709145 1.2739159 11.096105 85-89
ATCAA 1953950 1.2721338 9.372507 80-84
TGATA 1653210 1.2660403 11.388084 15-19
TGGCC 611490 1.2604903 38.76511 1
CCTCT 807460 1.2516799 9.001859 2
GGATA 1262715 1.2515411 13.582074 70-74
TCCAA 1326545 1.2455081 5.3162103 3
GCCAA 1024125 1.2445084 17.24026 3
TCTTG 978550 1.2372293 6.6854997 4
CAACA 1485395 1.2182151 6.3401713 8
CGGAA 975270 1.2176636 10.049589 5
TGTAG 1054945 1.1970495 6.173757 3
GCTGG 560280 1.1866235 7.1867824 1
CTTTT 1183820 1.1564652 5.3794775 6
GCAGT 797855 1.1404308 5.2079277 4
ATATC 1478545 1.1020375 9.312708 85-89
CCGAA 906440 1.1014984 34.68415 4
CCCAA 926010 1.0952237 8.88368 3
CCTTT 877050 1.0792794 5.513489 4
AATAT 2063215 1.0663534 7.4051437 80-84
CCAAC 895715 1.0593929 5.5922546 5
GCCCA 603625 1.0578333 8.674359 2
CCCTT 663055 1.0278312 5.013982 4
CCATA 1092195 1.0254742 5.4319186 3
TCAAC 1076060 1.0103248 7.4046326 7
TGCCA 710165 0.9879765 5.5214186 1
TTGAC 877975 0.96963173 6.3596196 6
GTTGC 581690 0.9518731 7.9667544 1
CGAAC 748910 0.91006935 33.0376 5
GAACT 943245 0.90992725 28.377495 6
CTCCG 448340 0.8994976 5.602933 2
CCTAA 947005 0.88915366 11.22576 4
ACCTA 941955 0.88441217 8.284997 3
TAAGT 1151925 0.88215256 5.1155834 5
GTAGC 616265 0.8808714 5.147165 8
GGTGC 413810 0.8764129 9.002508 2
GGCCT 423835 0.8736691 8.007963 2
TCCCC 445290 0.8695165 5.2967544 1
ACCGA 707700 0.8599912 21.334974 3
ATTGG 754915 0.85660446 5.850019 6
GACCT 615170 0.8558201 12.131374 2
GCTGT 511095 0.8363521 10.44103 1
GTCCA 600925 0.8360026 5.9749026 2
CTAAA 1281945 0.8346199 6.557326 4
GTGCA 578415 0.8267696 6.770722 3
CCCCC 335425 0.8250741 5.703045 1
CTGTC 517320 0.8239277 9.317195 4
TGTCC 516030 0.8218732 9.966623 1
TGCCC 408640 0.8198481 9.389833 1
GGCTG 386760 0.81912327 11.555578 1
GGGTG 360310 0.7840463 10.154724 1
GGGGT 351550 0.76498425 5.8888316 1
GTAGA 771760 0.76493067 5.587609 4
TGGCT 462855 0.75741243 7.5228777 1
GCAGG 408675 0.7560375 6.673458 1
TGTCA 675675 0.7462125 7.0376563 5
GGGCC 278755 0.7436919 11.389836 1
AAGTC 752215 0.7256449 6.2265124 6
CCGTC 361055 0.72437906 5.94235 4
GTCAA 743575 0.7173101 7.353714 6
GACCG 386655 0.6961956 31.057146 2
AGTCT 628595 0.6942177 8.419369 7
TGGGC 326630 0.6917734 5.0090623 1
GCCAG 384050 0.6915052 5.5875087 2
GGCCG 252320 0.67316586 21.172695 2
TCTAA 882065 0.65744954 6.0390434 9
CTAAC 680170 0.6386193 10.347363 5
CCCCG 245220 0.61974216 6.079442 2
TGCGG 290685 0.61564505 16.060005 3
TCCGA 440885 0.61335605 5.0266123 3
GCCCC 242490 0.61284274 6.233593 1
GGCCC 234360 0.60855 9.062032 1
GCCGA 332750 0.5991364 14.827672 3
TAACT 803295 0.5987382 8.517649 6
TGACT 535990 0.5919451 12.682729 1
GACCC 331850 0.5815564 6.908368 2
CTGTA 515785 0.5696306 5.68033 2
GTCTA 514240 0.5679243 8.449365 8
CAGGG 299665 0.554372 5.216665 2
GACTT 499570 0.5517229 8.999997 6
GACTG 380770 0.5442616 8.269304 2
GCGGA 276310 0.51116586 13.228582 4
CCCGA 286805 0.50261647 5.9416847 3
CCCGT 248555 0.498672 6.070233 3
GCCCG 191660 0.4976733 7.080293 2
GTCCG 220600 0.45473215 6.6407986 2
GGACC 248840 0.44805142 6.028647 1
GACTA 388395 0.37467593 5.746612 2
Produced by FastQC (version 0.10.1)