FastQCFastQC Report
Thu 31 Jul 2014
13S15A_GGCTAC_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 13S15A_GGCTAC_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9363930
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 241644 2.5805831525865743 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 133933 1.4303075738498685 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 110455 1.1795795141569831 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 96179 1.0271221591788917 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 72733 0.7767358363422195 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 71071 0.7589868783726491 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 69836 0.7457979715781728 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 62621 0.6687469897788642 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 50007 0.5340385927703432 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 42231 0.45099653671054785 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 41617 0.444439460781958 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 36710 0.39203624973702283 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 36405 0.3887790703262412 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 33012 0.3525442842908907 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 32208 0.3439581457785353 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 28386 0.3031419500145772 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 28206 0.30121968019837825 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 27568 0.29440630162762854 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 27447 0.2931141091400726 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 27241 0.2909141781282004 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 24820 0.2650596491003243 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 24489 0.26152480849386955 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 23442 0.2503436057296456 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 22503 0.24031576485514095 No Hit
AACAAGTTTATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATT 22201 0.23709062327462935 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 21732 0.23208204247575537 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 21375 0.22826954067362742 No Hit
ATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGAC 21351 0.22801323803146753 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 20718 0.22125325584450117 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 20647 0.22049502719477826 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 20488 0.21879702219046918 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 20062 0.2142476502921316 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 19945 0.21299817491160228 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 19838 0.21185549229863956 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 19353 0.206676043071659 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 17947 0.19166097995179374 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 17914 0.19130856381882394 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 17877 0.19091343057882748 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 17764 0.18970667230532479 No Hit
ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC 17744 0.18949308677019158 No Hit
TACCATGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTC 17530 0.18720772154426613 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 16886 0.1803302673129765 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 16728 0.17864294158542407 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 16718 0.17853614881785745 No Hit
ACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGACTG 16211 0.17312175550223036 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 15514 0.1656782996028377 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 15325 0.16365991629582877 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 15177 0.16207938333584299 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 14994 0.160125075689374 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 14809 0.15814940948939174 No Hit
CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA 13981 0.14930696833487647 No Hit
AGTATGCTTCAAAAATACCTTTGGTATTACATTGACAATCTAACGAGGAT 13601 0.14524884316734532 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 13341 0.1424722312106135 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 12886 0.13761316028633278 No Hit
GTATGCTTCAAAAATACCTTTGGTATTACATTGACAATCTAACGAGGATT 12226 0.13056483762693655 No Hit
TATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGA 12185 0.13012698727991345 No Hit
CTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGC 11809 0.12611157921940894 No Hit
ACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGAT 11418 0.12193598200755451 No Hit
GCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCAAT 11389 0.12162628298161135 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 11042 0.11792057394705001 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 10958 0.11702351469949049 No Hit
CCTACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGC 10622 0.11343527770925242 No Hit
ATGCACCCATTCCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCT 10400 0.11106447826927368 No Hit
GGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGACTGCTACTT 10213 0.1090674535157781 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 10174 0.10865096172226832 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 10104 0.10790341234930205 No Hit
CATCTTTTCAAGAGTTATTAATTGAGTTTTAGGGAGGGATTTATGTCACC 9784 0.10448604378717055 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 9728 0.10388800428879756 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 9658 0.10314045491583129 No Hit
GCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCT 9432 0.1007269383688259 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 9414 0.10053471138720602 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 9387 0.10024637091477617 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 4075830 3.9417162 8.559979 70-74
CTGCT 3024335 3.622073 20.22536 3
AGCTG 2673265 3.4584587 8.325286 80-84
ACTTC 3134775 3.3207705 8.038807 60-64
CAAGC 1906000 3.2541046 12.964316 30-34
CTTCT 3428015 3.0414767 6.677297 20-24
AACTT 3532410 2.9945483 6.6989603 60-64
AGCAC 1706915 2.9142077 9.445506 65-69
GGAAG 2077450 2.9032493 8.895328 15-19
CTGGT 2635295 2.85548 14.870412 1
GCTAC 1965775 2.810941 21.737694 5
ACATC 2220790 2.8088632 8.867564 90-94
CTACT 2640360 2.7970204 17.018982 6
CGGTG 1912200 2.7968469 8.933788 9
CTGAA 2395475 2.7411785 7.9554935 80-84
ACCCA 1431575 2.7014697 54.18557 1
ACAAC 1783360 2.693102 10.687246 85-89
GGTGT 2744725 2.6907377 9.471973 9
TGCTA 2779700 2.6641152 15.461665 4
CAACT 2103330 2.6602995 11.959997 9
TCTCT 2989635 2.6525278 9.008868 20-24
GCTGC 1638360 2.6486351 9.719981 3
TGGTG 2677780 2.6251094 8.551072 8
CTTCA 2467575 2.6139836 7.8864717 60-64
AGGAA 2096335 2.5913115 8.220389 10-14
ACTGC 1803545 2.5789618 23.006222 2
TCAAG 2247120 2.5714138 7.1147876 10-14
CAGCA 1503930 2.5676525 10.361673 60-64
GGTTC 2369330 2.5672932 6.2309885 90-94
GGTGG 1883460 2.4923809 5.059371 10-14
TTCAA 2936400 2.4892898 10.59594 9
CTTTA 3503190 2.4873292 10.437792 9
AAGCT 2165450 2.4779575 7.2860312 75-79
CCAAG 1442790 2.4632685 9.952393 75-79
CAACA 1631155 2.4632528 10.108342 85-89
GCAGC 1235995 2.3857238 7.158359 1
TATGC 2488620 2.3851385 7.9134865 8
GAAAG 1927140 2.382167 7.9955745 10-14
CCAGC 1110280 2.3687181 11.06412 60-64
GCTTC 1961500 2.3491764 9.452648 15-19
TCTGC 1954790 2.34114 6.8280387 45-49
TCAAC 1839850 2.327049 9.435348 8
GAAGC 1484445 2.292954 9.763808 15-19
CCGCC 854975 2.2793264 12.910467 75-79
TGAAA 2480190 2.2712157 6.926921 70-74
ATGGT 2617615 2.2697783 5.534302 90-94
CTTGG 2034310 2.2042816 9.872671 6
GCTGG 1501965 2.1968238 7.0643435 2
GATGG 1865265 2.1832511 6.825293 90-94
ATCGG 1674550 2.1664002 8.616243 85-89
CAGCT 1489165 2.1294172 6.5286427 2
CCGTG 1310745 2.1190002 5.2196817 60-64
TCGGA 1627220 2.105168 8.981207 85-89
ATGCA 1837645 2.1028454 6.21704 1
TCCTT 2365830 2.0990622 5.6611967 90-94
CTCTG 1751725 2.0979407 9.4008045 20-24
CAAGG 1356600 2.0954776 9.334795 10-14
GTTCC 1743140 2.0876591 6.748238 70-74
TTCCA 1957240 2.0733688 7.03463 75-79
CGTGA 1600740 2.0709107 10.184081 9
CTCCT 1551375 2.0536265 6.1544333 70-74
ACTGA 1767905 2.0230408 6.0262556 65-69
CATGG 1561575 2.020242 13.075287 2
TTCGG 1859545 2.0149145 8.386239 95-97
GCCTT 1674900 2.0059319 6.7948346 35-39
TCGGT 1850490 2.0051026 8.565383 8
ACCAG 1171690 2.0004206 9.069945 60-64
GCTCC 1109355 1.9822606 5.0237904 65-69
GAAAA 1808600 1.977455 6.575477 70-74
CACAA 1304150 1.9694333 8.766597 85-89
CGGAA 1272645 1.9657961 5.290521 95-97
CCTTA 1854430 1.9644588 10.139608 95-97
AAAGA 1772700 1.9382033 6.203229 15-19
CTCTC 1449975 1.9193988 5.6184936 95-97
AGCCT 1331415 1.9038441 7.6085577 30-34
AGAAA 1737445 1.8996569 12.562643 9
AAACC 1254825 1.8949466 8.234654 70-74
GCAAG 1225050 1.8922783 9.201755 25-29
TTTCA 2644700 1.8777857 9.075611 8
GGTAT 2164290 1.8766924 6.8670897 6
CATCC 1187155 1.8763021 9.142664 90-94
AAGGA 1516150 1.8741361 8.026252 10-14
GGCTC 1159240 1.8740715 6.4039884 90-94
TTGGT 2570990 1.8671854 23.23769 7
CTATG 1936025 1.8555218 7.185237 35-39
TGCTT 2309020 1.853498 6.9087796 4
ACCGC 866750 1.849161 10.132623 75-79
CCCAA 979320 1.8480368 53.886135 2
CACCC 783150 1.8467289 9.210771 50-54
GCACT 1291075 1.8461603 7.200849 65-69
CCACA 971870 1.8339784 9.211301 1
CACTG 1279195 1.8291726 7.7760897 65-69
CCTGT 1525850 1.8274232 6.1543436 1
TACTT 2559790 1.817498 12.256915 3
GGTCG 1242140 1.8167951 11.275556 6
GACTG 1398965 1.8098701 19.252409 1
TCTGG 1655690 1.7940269 6.4642944 45-49
GCACC 840665 1.7935101 8.442838 50-54
GTGTA 2060855 1.7870023 7.287404 2
CTTTC 2010325 1.7836436 11.143085 7
AAGCC 1041775 1.7786171 8.605877 30-34
TGGTA 2036275 1.7656887 11.675148 2
GCTTG 1617530 1.7526785 6.7942533 5
AAAAC 1443435 1.7443706 7.0068192 70-74
CCTTT 1955995 1.7354398 5.6034293 75-79
ACACA 1146435 1.7312635 8.576216 85-89
CATGC 1205720 1.7241077 8.802368 2
TCCAA 1361570 1.7221187 7.4410343 75-79
ATGGG 1471195 1.722001 10.616325 3
TGAAC 1492860 1.7083024 7.5413795 80-84
CCAAT 1347880 1.7048037 36.222343 3
TTACA 2007970 1.7022269 5.5761423 80-84
GAACA 1243400 1.6988194 8.100704 80-84
AAAGC 1243160 1.6984915 8.069462 20-24
ACTTT 2390500 1.697299 12.6300125 4
GCTGA 1310585 1.6955311 7.685111 80-84
GGATG 1446150 1.6926863 7.0217633 85-89
GGGTC 1154805 1.689056 11.279695 5
CTGCG 1043820 1.6874789 9.133689 45-49
TTTAG 2615310 1.6800234 8.527095 6
ACGGT 1294070 1.6741652 10.256115 4
CATGA 1458370 1.6688352 6.48826 65-69
AACAC 1103845 1.6669471 8.659691 85-89
GCGAC 861755 1.6633638 9.341377 50-54
CTCTA 1544165 1.635785 6.815377 95-97
TACCA 1265000 1.5999768 6.2762465 60-64
AGCTT 1668610 1.5992264 5.970854 15-19
ATGCC 1115625 1.5952773 10.651293 9
AACAT 1566305 1.5853574 6.190222 90-94
CTGGG 1082485 1.5832783 7.244956 55-59
GACGC 819975 1.5827198 9.840126 15-19
ACATG 1370985 1.5688392 14.03064 1
GCGCA 812205 1.5677222 9.870825 25-29
TGGGT 1593475 1.5621321 8.178522 8
GGTAC 1197910 1.549761 15.756946 3
CATCG 1081530 1.5465235 6.989131 85-89
CTGTA 1599315 1.5328125 7.015008 8
GACGG 877335 1.5321155 13.57416 3
AGCGC 792065 1.5288478 9.349228 25-29
CGCCT 851260 1.5210813 9.05127 75-79
ACTGG 1173315 1.5179421 7.3431306 50-54
TTAGG 1739610 1.5084453 6.685113 35-39
TGCAC 1053515 1.5064636 5.8808117 50-54
TGGGG 1132085 1.4980869 6.9016395 35-39
TTCAT 2088220 1.4826746 5.1476827 60-64
AACCG 865005 1.4768189 9.293239 70-74
CGCAA 861630 1.4710567 8.692459 25-29
GTGTT 2017215 1.4650054 5.40284 70-74
GCGAA 948305 1.4648031 7.657297 20-24
CGCGA 747300 1.4424422 9.13119 20-24
GCAAC 843870 1.4407352 10.046258 1
GGGGT 1080050 1.4292289 7.347615 35-39
ATCTC 1340110 1.4196228 6.7634373 45-49
TCGCT 1178910 1.411913 6.262184 40-44
ATGCT 1456185 1.3956343 6.9619617 3
AGACG 903120 1.3950077 8.079003 15-19
AAGCG 899355 1.3891921 7.544104 25-29
TCATG 1444760 1.3846844 5.590278 60-64
AAGAC 1012640 1.3835391 6.982684 15-19
GTCGC 852550 1.3782648 8.120732 40-44
GTCAA 1189375 1.3610201 6.700545 10-14
TGGTC 1246265 1.3503934 28.325956 8
GGAAT 1296575 1.3423506 6.1723013 40-44
GGTCA 1036175 1.3405212 33.49208 9
ACGCG 692830 1.3373039 10.421896 15-19
GCGGC 610580 1.3324189 6.8321867 7
TTCTC 1499575 1.330485 5.78753 20-24
CGACT 916435 1.3104475 7.261488 50-54
GTATG 1497040 1.2981088 6.9288764 7
CGGAT 1002630 1.2971233 6.4332323 85-89
TTACC 1222280 1.2948015 5.1787887 60-64
GCCTA 904830 1.2938529 7.169811 30-34
GTTAC 1349610 1.2934909 5.6391835 55-59
CGAAA 946040 1.2925454 7.100093 20-24
ACAAG 942205 1.2873058 5.172232 2
GGCTG 876475 1.2819612 5.2251425 9
CACAT 1012165 1.28019 6.216766 2
TGCGA 986900 1.2767731 6.3757963 50-54
GTACT 1329425 1.2741452 11.773945 4
GAATC 1113415 1.2740977 6.5002737 40-44
CAATT 1498600 1.270416 24.647093 4
GGGTT 1295480 1.2699986 5.4132285 55-59
TCTGA 1322660 1.2676616 5.76976 20-24
CTGAC 886265 1.2673061 11.452567 1
TGCCT 1053870 1.2621598 6.700137 30-34
ATCCT 1170105 1.2395308 6.0336714 90-94
GATTG 1408455 1.2212952 5.081245 65-69
TGTAT 1898110 1.2193084 5.2762947 9
TAGAA 1322410 1.2109872 10.360256 8
TGACG 929480 1.2024876 10.034128 2
CTTAT 1693080 1.2021179 6.6404862 95-97
CACGG 605705 1.1691349 5.862537 4
CGCTT 970680 1.1625279 6.741719 40-44
GTTCG 1057825 1.1462088 5.9456983 90-94
TAGGA 1100745 1.1396068 5.828698 40-44
ATTCG 1184235 1.1349925 5.248713 6
ATTGG 1296065 1.1238399 23.189983 6
CGGTA 836935 1.0827601 9.8221655 5
TGGCC 668125 1.0801164 7.5088296 1
TTATG 1641190 1.054268 6.08804 95-97
GCTCA 731085 1.045408 5.4694486 1
CCTAT 973305 1.0310541 5.876105 30-34
TTAGA 1339240 1.0271673 9.216648 7
AATTG 1309870 1.0046414 20.284155 5
TCGTG 919630 0.9964672 8.502356 8
CTACA 708200 0.895734 6.345945 3
TGACT 771860 0.73976475 8.158371 1
GTCGT 657535 0.71247363 8.315902 7
Produced by FastQC (version 0.10.1)