FastQCFastQC Report
Wed 6 Aug 2014
13L15C_CAGATC_L004_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 13L15C_CAGATC_L004_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14844847
Filtered Sequences 0
Sequence length 101
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 282327 1.9018518681937242 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 247823 1.6694210455655085 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 134394 0.905324251573627 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 117291 0.7901125555554732 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 100970 0.6801686807550121 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 83986 0.5657586097047682 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 79248 0.533841810562278 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 71012 0.4783612791698022 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 70944 0.47790320775956796 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 64327 0.4333288177372256 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 56880 0.3831632619723194 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 55878 0.376413445015634 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 50100 0.3374908478342687 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 47741 0.321599811705705 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 38685 0.260595478013347 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 36255 0.24422616144174472 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 35084 0.2363379023037422 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 34161 0.23012025654424056 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 32027 0.21574489787601045 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 31962 0.21530703549858077 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 31012 0.20890750844383912 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 30037 0.20233957278239378 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 29877 0.20126175769948992 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 28935 0.1949161213988935 No Hit
GCTGCTGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 27136 0.1827974380604933 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 27027 0.18206317653526508 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 26611 0.17926085731971506 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 25756 0.1735012829704476 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 24044 0.16196866158337636 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 23961 0.16140954500912 No Hit
GCTGCCGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 22950 0.1545991009540213 No Hit
GCAGAGGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 20720 0.13957705323604885 No Hit
ACCCAGTTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 20458 0.1378121310377938 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 19713 0.13279355455802272 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 18456 0.12432596981295935 No Hit
GCAGATGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 17963 0.12100495208876183 No Hit
GCAGTAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 17756 0.119610528825255 No Hit
ACAGGGGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 16446 0.1107859178339797 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 16167 0.10890647778316612 No Hit
GCTGTGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 16137 0.10870438745512163 No Hit
GACCACACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 15776 0.10627256717431982 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 15415 0.10384074689351801 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 15363 0.10349045699157425 No Hit
GCAGACGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 15102 0.10173227113758733 No Hit
TAGATGAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 14961 0.10078244659577833 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AGCAG 6181000 4.907683 54.48278 20-24
CACGG 4049290 4.457119 44.833595 45-49
TAGCA 7081610 4.344902 34.00666 20-24
TTTTT 8485375 4.189676 7.645769 7
ACTTG 5905285 4.0949807 101.881454 8
CCTGC 3381045 3.9867055 48.92252 15-19
GCAGA 4960870 3.9389055 36.101986 25-29
CTGCG 2985195 3.7137356 50.873756 15-19
GTTTC 4734895 3.7109423 35.536125 40-44
TCACG 4265205 3.6278186 34.71996 45-49
CGGAT 3825630 3.4330773 34.257076 45-49
GAACC 4512090 3.3956306 30.82248 70-74
GAAGT 5154665 3.3367484 27.630106 7
AGAGA 5681230 3.2538872 21.817734 90-94
CTTGA 4558325 3.1609397 28.971607 55-59
AACTT 6629260 3.1429918 69.795654 7
ACGGA 3917695 3.11063 32.06847 45-49
GCAGC 2815675 3.0992587 18.345583 20-24
AGAGC 3901465 3.0977433 52.20403 95-97
AGTAG 4710015 3.0489147 27.591469 9
GCGTT 2914440 2.9559608 39.0846 15-19
TTCTC 3962860 2.9437947 29.260109 30-34
GAGCC 2621220 2.885219 64.60871 95-97
CCATG 3375585 2.8711417 34.806458 75-79
TGCGT 2824715 2.8649578 41.22065 15-19
ACCTG 3359635 2.8575754 28.22649 15-19
CATGC 3336265 2.837698 34.672718 75-79
AACCA 5472490 2.8157554 20.125475 70-74
CTTGT 3579470 2.8053858 115.36859 9
ATAGC 4461365 2.7372577 24.60407 80-84
ACCAT 4637760 2.6970015 29.999214 75-79
TTCAC 4088420 2.6871467 22.723423 45-49
TGCAT 3832120 2.6573577 27.007627 75-79
CACTG 3105685 2.6415753 33.526016 85-89
GAGAG 3113510 2.6082127 42.95139 95-97
TGAAG 4028800 2.6079466 21.321024 60-64
TTTCA 4865555 2.6071951 21.425999 40-44
AGCAC 3464100 2.6069524 28.538448 80-84
CTCAG 3041495 2.5869777 34.623783 35-39
GCACT 2998100 2.5500677 26.659765 85-89
AAGTT 5086980 2.5445635 21.229977 60-64
GCTGC 2031640 2.5274644 35.930817 1
GGAAC 3154635 2.504764 32.351276 70-74
TTGAA 4950290 2.4761896 19.75072 55-59
GACTC 2905605 2.4713953 34.509 25-29
AGGAG 2910600 2.4382334 18.974592 90-94
AGAAG 4212610 2.4127448 15.125436 6
GGATT 3234165 2.366182 23.747238 50-54
AGGAA 4076805 2.3349633 23.545568 70-74
CTGAA 3796415 2.3292797 24.32884 85-89
AAGGA 3976420 2.2774682 18.771807 70-74
ATGCA 3707655 2.2748215 25.389896 75-79
AAACT 5411945 2.2702236 30.550964 6
TTGTA 4010630 2.2674034 24.59898 9
GTTAC 3256840 2.2584338 26.590769 60-64
TTAGC 3256410 2.2581356 28.664148 20-24
GTAGT 3073750 2.2488189 23.694902 40-44
CAGAC 2976560 2.2400477 30.492704 25-29
ACAGG 2815775 2.235711 13.149684 60-64
CATTC 3394150 2.2308323 27.132715 30-34
GAAGA 3888320 2.2270098 8.323315 50-54
AGTTT 3926025 2.219572 23.505505 40-44
TCTCA 3347115 2.199918 21.52645 35-39
CAGTA 3570600 2.1907313 25.041233 35-39
TCAGT 3140405 2.177693 28.561916 35-39
GCATA 3519145 2.1591613 19.882332 80-84
GTAGG 2277000 2.155851 25.294514 7
GTTAG 2938970 2.150211 30.164333 20-24
ACCAG 2853250 2.1472495 12.213034 55-59
CTCAT 3264435 2.1455758 21.481176 30-34
GTTGT 2566200 2.121971 12.582318 35-39
CAGCA 2816365 2.1194909 15.7412815 20-24
TAACC 3618190 2.10409 23.635405 10-14
GCTTC 2175075 2.0909457 5.170732 15-19
TAGAG 3225955 2.0882447 25.257246 90-94
CATAG 3388445 2.078971 24.84058 80-84
GATGA 3210375 2.0781589 9.280066 15-19
GGAAG 2468380 2.0677822 10.894461 6
AACCT 3540135 2.0586982 22.648026 10-14
GGCTC 1649500 2.0520623 18.21381 65-69
AAGAG 3578305 2.0494504 9.166157 50-54
TGTTG 2470415 2.0427668 11.579899 35-39
TAAGG 3150535 2.0394235 24.813988 65-69
CGTTA 2918270 2.0236547 22.705647 20-24
TCATT 3767380 2.0187411 22.094337 30-34
TGTAA 4019265 2.0104806 20.895771 10-14
GGAGG 1640360 2.0098546 16.33993 90-94
CTAAG 3274990 2.0093608 24.861713 65-69
GTAAC 3251840 1.9951572 25.119574 10-14
CTGCA 2344110 1.9938092 16.04127 3
TTCCG 2022970 1.9447241 13.932046 40-44
TTTCC 2613615 1.9415134 10.927167 40-44
CAGGC 1761790 1.9392304 14.397797 65-69
ATTCT 3604605 1.9315186 22.254871 30-34
ACTGA 3136005 1.9240872 24.516571 85-89
CAGAA 3536165 1.9196299 11.338395 5
CCAGA 2539270 1.9109598 16.335852 55-59
TGAAT 3813695 1.9076523 19.09745 85-89
CCATC 2346220 1.8914691 13.322451 75-79
AGGGA 2254275 1.8884246 22.660652 9
AGTTA 3766720 1.8841549 20.260874 60-64
TAGTT 3325580 1.8801117 23.094296 40-44
AGACT 3055140 1.8744726 25.100595 25-29
GAATA 4208250 1.8624811 14.242783 90-94
ACTCA 3191360 1.8558751 22.522491 25-29
GGAGC 1595410 1.8527751 16.230669 95-97
TAGGG 1948565 1.8448906 25.396917 8
TTAAA 5383830 1.8412465 14.549424 50-54
AGCTG 2017845 1.8107916 8.056556 95-97
TGCAG 2005730 1.7999197 17.468098 4
TGACC 2112005 1.7963896 68.39344 1
TTACT 3330075 1.7844121 17.390402 65-69
AATAG 4015745 1.7772824 17.934418 90-94
CTTCC 1937705 1.7655544 5.050206 10-14
GGATG 1845990 1.7477732 16.70711 10-14
CCAAA 3374700 1.7363815 29.369373 4
ATGAT 3439435 1.7204435 8.0408125 15-19
GCGTC 1377225 1.7133384 5.9013395 95-97
GATGT 2336455 1.7093986 14.707774 30-34
TTGTT 2671205 1.7068146 10.198435 35-39
ATTAA 4985840 1.705136 14.433014 50-54
CAGAG 2133775 1.6942062 12.162085 25-29
TGATG 2309870 1.6899486 11.314125 30-34
GAGCG 1450185 1.6841229 7.7449355 90-94
ACCAA 3272125 1.6836036 19.089968 3
GATCG 1836150 1.6477405 5.8732567 95-97
CGTCG 1321500 1.6440138 6.1204076 95-97
TGTTT 2571830 1.6433172 13.241567 35-39
TAAAC 3904155 1.63773 16.472649 50-54
CCTCC 1452910 1.6237788 8.2666 1
GATTA 3231380 1.6163719 20.283611 50-54
TCCGT 1679135 1.6141882 14.250935 40-44
TACTA 3396675 1.6103944 19.300076 65-69
GAGAT 2480870 1.6059314 13.168455 25-29
CCACC 1611850 1.5938588 6.3285403 1
CAGGA 1998360 1.5866877 10.428941 90-94
AAGAA 4041645 1.5826524 8.787215 45-49
TCCTC 1735285 1.581118 6.7568064 1
ATCGG 1757555 1.5772103 5.761668 95-97
AGGCT 1753845 1.5738809 12.936301 65-69
GCTCA 1818005 1.5463247 16.779924 65-69
TCGGA 1720335 1.5438094 5.531091 95-97
ATAGA 3461830 1.5321317 17.734642 90-94
GAGCT 1690125 1.5166993 9.147915 95-97
CATCA 2602785 1.5136005 7.642248 80-84
CTACA 2590335 1.5063604 9.418007 85-89
ACTAA 3576580 1.5003177 17.284805 65-69
GGGGG 835945 1.4980829 7.2761955 1
CGGAA 1875495 1.4891335 9.084782 5
AGCGT 1650285 1.4809474 5.6968784 90-94
CTCAC 1830245 1.4755019 12.892955 65-69
GGGAT 1555330 1.4725779 18.78767 10-14
GAGGA 1745695 1.462383 14.618478 95-97
TACCA 2511595 1.4605707 8.825168 75-79
CTGCT 1513720 1.4551712 6.15886 2
TGCTG 1432380 1.4527867 5.665678 1
GTCGT 1431325 1.4517167 5.149077 95-97
AAAGA 3616850 1.4163086 6.554543 45-49
TAAAG 3186795 1.410407 7.4319882 45-49
TACAG 2294260 1.4076368 8.162369 90-94
AACAG 2590880 1.4064759 12.698154 60-64
GAAAC 2580435 1.4008058 8.093254 60-64
ATACC 2383145 1.385873 8.457086 75-79
GAGAA 2407720 1.3790058 11.929206 50-54
ATGTT 2431260 1.3745092 9.439769 30-34
GACCA 1824185 1.3728136 25.69304 2
AGCCA 1794065 1.3501464 11.174409 95-97
GTAAA 3050145 1.3499286 6.773347 45-49
AGAAA 3413690 1.336754 5.778648 60-64
TCTAC 2011010 1.3217523 11.6760025 85-89
GTGCT 1286945 1.3052796 18.348969 9
GCTCC 1102965 1.3005437 6.1549582 1
CAAAC 2517235 1.2951908 31.378294 5
AGATG 1981215 1.282492 10.6996975 25-29
CCGTA 1499535 1.2754463 16.702513 40-44
AAGTA 2796545 1.2376907 19.670755 8
GCTGG 928185 1.2182821 6.065743 1
AGAAC 2240170 1.2160908 10.0274315 50-54
TTGGT 1465315 1.2116576 13.065471 7
CCTTT 1617165 1.2013046 5.365788 4
GGCCA 1086010 1.1953887 15.403811 2
AAACA 3189825 1.1839113 6.040889 60-64
GCCAA 1572795 1.1836268 12.154105 3
CCCAA 1657685 1.1824142 10.743646 4
TGGCC 950250 1.1821597 31.549505 1
GATAC 1913860 1.1742433 7.6590285 75-79
CAATA 2794460 1.172231 7.807411 80-84
CCGAA 1552310 1.1682106 33.738224 4
CCTCT 1279400 1.1657349 5.9267488 2
TCAAT 2437975 1.155866 6.6347556 80-84
ATCAA 2645445 1.1097215 6.070975 80-84
CCAAC 1534145 1.0942941 8.665083 5
GCCCA 1048775 1.0941645 6.734029 2
TGATA 2186245 1.0935838 7.811382 15-19
TCAAC 1877965 1.0920951 5.0574636 7
GATAG 1681660 1.0885822 12.910497 15-19
CGTAA 1747575 1.0722197 9.8819065 40-44
CTCCC 950635 1.0624341 5.0325165 2
CCAAT 1825850 1.0617884 6.455976 3
CAACT 1806125 1.0503178 7.1697683 6
ATCTA 2201025 1.0435259 6.5751786 85-89
TATCT 1943660 1.0415053 7.137592 85-89
TGTGC 1025325 1.0399325 18.10636 8
GGGTG 743665 1.0298305 10.853549 1
ACCCA 1433735 1.0226723 8.122648 1
GGATA 1566480 1.0140233 10.053878 70-74
CCCTT 1110520 1.0118587 5.2033095 3
GTTGC 991450 1.0055748 5.3459167 1
TGGTC 989495 1.003592 15.330943 8
AATAT 2864590 0.9796773 5.3305225 80-84
GGTGT 915400 0.9795566 13.213977 6
GGTGC 735055 0.96479076 9.231518 2
ATTGG 1297430 0.9492267 11.242132 6
ACCGA 1255275 0.9446731 23.440582 3
CTGCC 789185 0.930555 6.062782 2
GAACT 1507645 0.9250114 28.025484 6
GTGTG 857520 0.9176201 19.202122 7
GTGCA 1011005 0.90726453 6.8730965 3
CTCCG 760700 0.89696735 6.2778187 2
CCCAG 855515 0.89254045 6.79222 2
GCTGT 878170 0.890681 7.645738 1
CCTAA 1528640 0.88895166 12.66882 4
GACCT 1043965 0.8879562 13.347776 2
GGCCT 711790 0.88550323 7.899016 2
CGGTG 673770 0.8843516 6.9226856 5
GGCTG 673770 0.8843516 7.1505475 1
CCAGT 1034805 0.8801651 5.4921665 3
ATATC 1845100 0.87477857 5.759669 85-89
CGAAC 1158265 0.8716669 31.571907 5
CCGCC 596075 0.8621023 5.8722444 1
GCAGG 741860 0.86153376 6.6815495 1
GGGGT 616240 0.85337174 6.0562387 4
ACCTA 1463105 0.850841 9.36228 3
CCGTT 882545 0.8484092 6.395583 4
TGCCC 709365 0.8364365 7.7437406 1
TGTCC 853240 0.82023776 6.3976903 1
GTCAA 1319710 0.8097043 5.359055 6
TCCCC 722015 0.80692726 5.8061347 1
CCCCC 586720 0.8042921 7.6569085 1
CTGTC 833945 0.801689 6.2254014 4
CAATT 1664795 0.78929436 5.0724363 4
CCCCT 696115 0.7779813 5.044987 3
CCGTC 639830 0.7544453 6.706603 4
GGTCA 828475 0.7434642 13.414533 9
GGGCC 460440 0.741278 9.736628 1
GACCG 643510 0.7083218 32.903072 2
CGCCC 483975 0.6999723 5.7200193 1
AGTCT 990135 0.6866024 5.9305377 7
TGCGG 512070 0.6721136 14.452389 3
GGCCG 411720 0.6628419 18.033827 2
CAGGG 565610 0.6568518 6.4493766 2
TAACT 1379860 0.65420413 9.939807 6
CTAAC 1124150 0.6537282 11.540079 5
GGCCC 414385 0.6323201 9.0376 1
GCCCC 430170 0.6221542 6.5618176 1
GACCC 594505 0.6202343 10.837293 2
CCCCG 425745 0.6157543 8.215819 2
GCCGT 491340 0.6112521 5.8758435 3
GGTAG 642310 0.6081355 5.8503466 6
TGACT 864745 0.5996516 10.074759 1
GCCGA 541365 0.5958892 11.9058895 3
GACTG 654710 0.5875294 6.723365 2
GACTT 825490 0.57243055 7.534403 6
TCCCG 483915 0.5706007 5.4045177 2
GCGGA 464745 0.5397157 11.421064 4
CCCGA 514165 0.5364173 6.8802714 3
GTCTA 771135 0.53473836 5.9990196 8
GCCCG 346535 0.5287861 8.453508 2
CCCGT 438945 0.517575 7.8111053 3
GTCCG 370165 0.46050423 5.572333 2
Produced by FastQC (version 0.10.1)