FastQCFastQC Report
Wed 6 Aug 2014
13L15C_CAGATC_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 13L15C_CAGATC_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14844847
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 271296 1.8275432545717716 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 138242 0.9312457043174646 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 108043 0.7278148437636306 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 91294 0.6149878136164016 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 89856 0.6053009505588033 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 81995 0.5523465482668835 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 64595 0.4351341580010895 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 63075 0.42489491471350294 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 62327 0.4198561292009274 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 61932 0.4171952732150086 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 46257 0.3116030768117718 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 43748 0.29470158904298577 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 41561 0.2799692041285437 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 39910 0.2688474997418296 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 38235 0.2575641230926799 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 37992 0.2559271914355197 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 37765 0.25439804128664983 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 37752 0.25431046881116387 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 35334 0.23802198837077942 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 34026 0.22921085006804043 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 32628 0.21979344078116803 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 31302 0.21086104828160235 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 30773 0.20729752216375152 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 29191 0.1966406255315397 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 29126 0.19620276315410998 No Hit
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 27813 0.18735794313003024 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 27250 0.1835653813070623 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 27121 0.18269639289647108 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 26279 0.17702439102268955 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 25667 0.17290174833058233 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 24633 0.16593636835731618 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 24047 0.1619888706161808 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 23530 0.15850618062954774 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 23337 0.157206066185795 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 23207 0.1563303414309356 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 23180 0.15614846013569555 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 23009 0.15499654526584208 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 22802 0.15360212200233522 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 21819 0.14698029558674466 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21411 0.14423186712533986 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 19690 0.1326386186398553 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 19197 0.1293176009156578 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 18559 0.1250198132725787 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 17805 0.1199406096943943 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 17724 0.11939496580867422 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 17649 0.11888973998856305 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 17425 0.11738079887249765 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 16966 0.11428881685341721 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 16832 0.11338614672148524 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 16281 0.1096744210297351 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 16188 0.10904794101279723 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 16173 0.108946895848775 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 15986 0.10768719947063113 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 15749 0.1060906858790798 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 15225 0.10256084148256966 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGCT 4372665 3.3717904 7.5411506 85-89
ACTTC 4720035 3.2289996 7.57718 60-64
AAAAA 5321380 3.1339116 4.782763 45-49
AACTT 5656135 3.0573192 7.8934093 7
AGCTG 3774535 3.0439627 7.210001 3
GGAAG 3493415 2.9463713 7.1814246 15-19
AGCAC 2695405 2.866036 6.9356127 80-84
CTGGT 4032010 2.7690184 14.690033 1
GAAGA 3559100 2.6630905 5.1621184 95-97
TCTCT 4566130 2.6601088 8.801743 20-24
CTTCA 3863240 2.6428616 7.8031573 60-64
TCAAG 3689450 2.6396468 6.13736 10-14
CTACT 3853895 2.6364686 9.264231 2
GCTGC 2531775 2.5840604 8.298818 3
CTTCT 4407675 2.567797 6.3746657 20-24
TTCAA 4694270 2.5374005 8.753932 9
CGGTG 2776575 2.5239275 12.252425 9
GGTGG 3109200 2.5171323 10.00168 7
CAACT 3130710 2.5149953 10.097841 9
AGGAA 3344865 2.5027895 7.0153694 40-44
GGTGT 4089665 2.5013926 10.71133 1
CTGAA 3495660 2.5009983 6.8667445 80-84
TGGTG 4050705 2.4775631 8.479314 6
CAAGC 2315795 2.4623947 8.761262 75-79
ACAAC 2518690 2.3759685 7.908199 85-89
ACCCA 1985600 2.3706067 39.19915 1
CAACA 2498630 2.3570452 7.729767 85-89
GCAGC 1924525 2.3066025 6.3902287 1
TCAAC 2869865 2.3054504 9.023403 8
AAGCT 3200785 2.2900274 6.120269 15-19
TATGC 3723610 2.2686985 7.8673477 30-34
CCAAG 2126080 2.26067 8.522101 75-79
CAGCA 2125980 2.2605636 5.848626 2
GAAGC 2354705 2.2298927 8.076394 15-19
CGGAA 2349970 2.2254086 6.2995324 95-97
ATGCA 3109905 2.2250068 5.285946 1
TCTGC 2883755 2.2236822 5.4971614 85-89
CCGTG 2172695 2.217565 5.999998 60-64
ACATC 2757470 2.2151604 6.482561 90-94
GCTGG 2396560 2.178491 8.564945 4
AGAGC 2262880 2.142935 9.376518 95-97
ACTGC 2360880 2.137765 7.025376 4
CATGG 2619490 2.1124802 11.043372 2
TCCTT 3619680 2.1087315 6.3132954 40-44
CGTGA 2613685 2.1077986 8.969283 9
GTTCC 2732830 2.1073031 5.8169293 70-74
CAAGG 2220205 2.102522 7.80815 10-14
CTCTG 2723710 2.1002705 7.849289 45-49
CAGCT 2260535 2.0469027 5.1320524 2
TCGGA 2517490 2.0302224 5.057109 95-97
ATCGG 2513710 2.027174 5.2116976 95-97
GGCTC 1943450 1.9835855 5.397024 90-94
CTTGG 2879925 1.9778138 6.6107316 2
GCTAC 2169470 1.9644438 11.588053 1
AAGGA 2606030 1.9499574 6.6641345 10-14
GAGCA 2046885 1.938389 5.236886 90-94
CCAGC 1439875 1.9376892 7.2820697 1
CATCC 1898655 1.9303771 8.009377 90-94
CCTTA 2810940 1.9229779 8.4690485 95-97
TGGCT 2788515 1.9150372 7.341152 9
TTTCA 4141775 1.9064959 7.590386 8
TTCCA 2786045 1.9059471 6.023554 75-79
GATCG 2362130 1.9049329 5.474212 95-97
TGCTG 2754535 1.8917013 5.62196 6
GTGGC 2061035 1.8734964 8.961601 8
GCTTC 2413630 1.8611658 8.686666 15-19
TAGCT 3011425 1.8347828 5.1713347 2
CACAA 1935020 1.8253721 7.4476614 85-89
CTCCT 2106460 1.823802 5.600087 70-74
AGATC 2548200 1.8231305 5.70478 95-97
GCACA 1713070 1.8215147 5.5598416 95-97
TTCGG 2620355 1.7995518 9.189491 7
CTCTA 2598760 1.7778245 6.010731 95-97
AAACT 2792255 1.7723427 5.069927 6
TCTGG 2576455 1.7694031 5.663136 45-49
TATTC 3814665 1.7559242 5.6561503 5
TCGGT 2535695 1.7414109 10.090704 8
CCCAA 1452975 1.734706 38.435146 2
CACCC 1146325 1.7321172 8.035242 50-54
GCTCT 2238810 1.7263609 5.477158 10-14
GCAGG 1610050 1.7186142 5.374678 90-94
GGTAT 3162875 1.7162656 5.609495 30-34
TTGGT 3694850 1.7073702 18.110977 7
CATGC 1871410 1.6945522 5.240041 2
CCTGT 2189000 1.6879522 7.123975 1
TGAAC 2353555 1.68387 6.5114064 80-84
CTTTC 2882885 1.679494 9.323527 7
GCACC 1244385 1.6746116 7.3556633 50-54
CACAC 1399780 1.6711963 6.171882 95-97
GCCTT 2128215 1.6410804 5.85899 35-39
GCTGA 2022705 1.6312045 6.476733 80-84
CCACA 1358910 1.6224018 6.6968746 60-64
AATGA 2866705 1.6205612 5.203795 75-79
GTGTA 2983400 1.6188774 9.097692 2
TGTAG 2947715 1.5995138 5.9311433 3
TGGTA 2946860 1.5990497 10.409049 2
CCGCC 936845 1.5956175 6.7580857 45-49
TCCAA 1978320 1.5892451 6.4050555 75-79
GAACA 1887190 1.5855191 6.7857704 80-84
GCTTG 2305175 1.5830992 6.4079747 1
AGCTT 2590910 1.578574 5.2697654 15-19
ACACA 1670165 1.575525 7.209752 85-89
GTAGC 1941535 1.5657451 6.1196547 1
CCAAT 1946755 1.563888 26.230793 3
AACAC 1619625 1.527849 7.234824 85-89
TACTT 3272220 1.5062319 7.848054 5
ATGCC 1660585 1.5036511 6.820551 30-34
CGCAG 1252130 1.5007164 6.1241894 90-94
CATGA 2081890 1.4895053 5.5498037 65-69
CCTCC 1151920 1.4822456 5.171373 1
AACAT 2327450 1.4773145 5.0339036 90-94
ACGGT 1829275 1.4752134 6.428523 4
GTCTG 2133315 1.465073 5.648514 90-94
GCAAC 1362610 1.4488691 5.3944893 1
GCTGT 2104190 1.445071 5.6609106 7
GGTAC 1788840 1.4426048 12.939924 3
TTCTC 2447525 1.4258646 5.534954 20-24
TTAGG 2626875 1.4254168 5.438305 35-39
ACCAA 1510605 1.4250065 5.021069 3
GAGCC 1188905 1.4249393 6.9418263 95-97
ATCTC 2078000 1.4215701 6.01503 45-49
GACGG 1326165 1.4155872 8.35886 3
AGCCT 1555915 1.408873 5.8309956 4
CTGTA 2293590 1.3974246 5.0239444 5
GTCAA 1933105 1.3830557 5.7400327 10-14
GGTCA 1713495 1.3818431 27.336712 9
TGCAC 1518250 1.3747675 5.1561446 50-54
ACTTT 2962840 1.3638217 7.7916555 6
ACATG 1899700 1.3591559 9.654482 1
GTATT 3301915 1.3536462 5.6704116 4
CTGCG 1320355 1.3476226 5.9358516 15-19
TGGTC 1953270 1.3414253 23.529217 8
GGAAT 2077595 1.3238384 5.193661 40-44
ATGGG 1826500 1.3118536 9.530855 3
GCCTG 1274425 1.3007442 6.079269 3
GCGGC 946715 1.278969 5.375379 7
GGTCG 1388050 1.261748 9.46262 6
ACACG 1164215 1.2379148 5.258915 95-97
TGGGT 2019085 1.2349482 7.3590846 4
TGCCT 1584560 1.2218646 5.743127 30-34
CAATT 2255010 1.2189039 17.774458 4
CTGAC 1344690 1.2176098 7.3418508 1
ATCCT 1767715 1.2093025 5.2406516 90-94
GAATC 1687515 1.2073463 5.5203753 40-44
AAGCC 1135435 1.207313 5.59965 1
GTACT 1972875 1.2020214 9.76376 4
TGTAT 2911320 1.1935188 5.3403187 3
ATTCG 1923100 1.1716946 7.250657 6
AACGC 1101345 1.1710649 5.036356 95-97
TGACG 1445950 1.1660821 6.257272 2
GGGTC 1261865 1.1470448 9.457265 5
ACTTG 1874030 1.1417978 8.300354 8
ATTGG 2082985 1.1302867 18.172606 6
TGGCC 1090035 1.1125462 11.092957 1
GCTCA 1217195 1.1021638 5.166293 1
CTTAT 2367390 1.0897306 5.7570615 95-97
TGGGG 1323300 1.0713112 5.172542 8
CCAAA 1106180 1.0434983 6.188993 4
TTATG 2453560 1.0058562 5.125468 95-97
CGGTA 1225650 0.9884218 5.9840307 5
CAAAC 1035825 0.97712994 6.0339713 5
TCGTG 1420525 0.97555804 7.204649 8
AATTG 2018680 0.9718032 14.88809 5
CCCAG 684385 0.9210004 5.832441 2
GACCA 835045 0.88790697 5.1676784 2
GCCCA 653825 0.8798748 5.305303 1
GGCCT 789235 0.80553406 6.485859 2
GCCTA 888210 0.80426955 5.719064 3
GGCCC 500850 0.7597288 5.871909 1
CTTGT 1405965 0.72948354 6.9882345 9
GTCGT 1018015 0.6991308 6.94397 7
TGACC 733050 0.663773 7.9349065 1
Produced by FastQC (version 0.10.1)