FastQCFastQC Report
Thu 31 Jul 2014
13L15B_CAGATC_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 13L15B_CAGATC_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14232016
Filtered Sequences 0
Sequence length 101
%GC 46

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GCGAGTCGGGCTGTTTGGGAATGCAGCCCTAAGTGGGAGGTAAATTCCTT 226275 1.5899012480030938 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 199424 1.4012350744968247 No Hit
GATACCGTCCTAGTCTCAACCATAAACGATGCCGACTAGGGATTGGCGGA 139092 0.9773176196541656 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 83065 0.5836488660496165 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 77593 0.5452003426640329 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 73428 0.5159353390271624 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 67917 0.47721278559551933 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 67850 0.47674201602921185 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 64952 0.45637947568355736 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 64892 0.45595789099731204 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 61205 0.43005151202753006 No Hit
CATGGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAAC 55771 0.39186999227656855 No Hit
CAGACCTCTGATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTAA 49215 0.34580483889281743 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 46548 0.3270653995892079 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 44381 0.3118391660043103 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 44032 0.3093869484126493 No Hit
CAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATTG 38911 0.2734046954416015 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 37745 0.26521189970556525 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 37667 0.2646638396134462 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 37274 0.2619024599185386 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 36656 0.25756013765021063 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 36305 0.25509386723567484 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 35921 0.25239572524370407 No Hit
CAAACGAATAAGGGCTTATGGTGGATACCTAGGCACCCAGAGACGAGGAA 35658 0.25054777903566156 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 35337 0.24829230096424848 No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGT 35248 0.24766695034631778 No Hit
TGGGTCGGGGGGAGCGGTCCGCCCCTTGTGGGTGTGCACTGGTCCACTCG 34843 0.24482125371416108 No Hit
AGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGG 34300 0.2410059123036399 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 33589 0.23601013377163152 No Hit
GATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTAAGCGGAGGAG 33408 0.23473835330145779 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 30955 0.21750256604545695 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 30600 0.21500818998517146 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 30270 0.21268947421082157 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 30056 0.21118582216321288 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 29673 0.20849470658267952 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 28256 0.19853828157584985 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 28002 0.19675357307074415 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 26094 0.18334718004813935 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 24870 0.1747468524487325 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 24250 0.17039047735752966 No Hit
CTGATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTAAGCGGAGG 23437 0.16467800485890405 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 22003 0.15460213085763816 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 21856 0.15356924837633684 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 21652 0.15213586044310237 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 21541 0.1513559287735483 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 21528 0.1512645854248618 No Hit
AAGTGGGAGGTAAATTCCTTCTAAGGCTAAATATTGGCAAGAGACCGATA 21048 0.14789190793489834 No Hit
GAAACATCTTAGTAGCCAGAGGAAAAGAAAGCAAAAGCGATTCCCATAGT 20644 0.14505323771417908 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 20602 0.14475812843380728 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 20412 0.1434231102606967 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 20062 0.14096386625759835 No Hit
GACACTGAGAGACGAAAGCTAGGGGAGCGAATGGGATTAGATACCCCAGT 19747 0.13875054665480985 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 19420 0.1364529101147722 No Hit
CCACGAGGCGCGATCTGCGAGTCGGGCTGTTTGGGAATGCAGCCCTAAGT 19418 0.13643885729189736 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 18694 0.13135173541120246 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 18547 0.13031885292990117 No Hit
TACCATGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTC 18243 0.1281828238529243 No Hit
CAACGCGAAGAACCTTACCAGGGCTTGACATGCCGCGAATCCTTTTGAAA 18214 0.12797905792123898 No Hit
TGGCAAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAGATGAAAAGGA 18012 0.12655972281087935 No Hit
ACATCGGTAGGGGAGCGTTCCGCCTTAGAGGGAAGCATCAGCGCGAGCAG 17946 0.12609597965600938 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 17932 0.12599760989588543 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 17821 0.1252176782263314 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 17726 0.12455016913977612 No Hit
AACACGGACCAAGGAGTCTAACATGTATGCAAGCAGGTGGGCGGCAAACC 17556 0.12335567919541406 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 17512 0.12304651709216739 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 17299 0.12154989145599611 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 17070 0.11994084323682605 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 17066 0.11991273759107633 No Hit
GCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATT 16295 0.11449537437282252 No Hit
GTAGGTGGCTTTTTAAGTCTGCTGTCAAATCCCAGGGCTCAACCCTGGAC 16243 0.11413000097807648 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 15837 0.11127727793448236 No Hit
ATCACTAAGCGGAGGAGAAGAAACTAACCAGGATTTCCCTAGTAACGGCG 15622 0.10976659947543622 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 15509 0.10897261498300734 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 15257 0.10720195930077651 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 14982 0.10526969615548493 No Hit
TAGTAGCCAGAGGAAAAGAAAGCAAAAGCGATTCCCATAGTAGCGGCGAG 14947 0.10502377175517509 No Hit
AAGCGGAGGAGAAGAAACTAACCAGGATTTCCCTAGTAACGGCGAGCGAA 14938 0.10496053405223828 No Hit
ACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCA 14931 0.10491134917217632 No Hit
GTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTGCT 14887 0.10460218706892965 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 14772 0.1037941497536259 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 14720 0.10342877635887986 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 14447 0.10151056603646313 No Hit
ATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGAC 14402 0.10119437752177908 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 14326 0.10066037025253484 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTTCT 4214305 3.2016098 8.190457 45-49
CTGCT 3799770 3.2001557 8.712735 5
ACTTC 3580065 2.9179807 5.899934 60-64
AGCAC 2722130 2.638887 6.7521677 70-74
CTACT 3176680 2.5891962 12.781209 2
TGAAA 4286730 2.524279 5.2408056 65-69
TCTCT 3267445 2.4822798 8.108311 20-24
CTGAA 3239210 2.3627956 5.8116384 80-84
CTGGT 3353800 2.3561206 8.421622 1
TGCTA 3334300 2.266954 5.8982587 4
AGCTG 2985270 2.2500546 5.3732743 3
TCCTT 2960225 2.248885 8.246255 45-49
CAACT 2519540 2.2032402 5.729711 55-59
GCAGC 2195060 2.198774 6.6498327 20-24
TTCAA 3337675 2.1961384 6.2536197 55-59
TCAAC 2499480 2.1856983 5.6430125 55-59
ATCGG 2888090 2.176808 6.100604 85-89
GGTGG 3289615 2.1370924 6.2717133 7
CAAGC 2203015 2.1356466 5.71938 2
CGGTG 2736005 2.130829 8.299354 9
TGGTG 3631805 2.128283 5.8496833 6
CTTCA 2609540 2.126941 5.659631 60-64
ACTGC 2342000 2.116166 7.4001718 2
GAAAG 3239125 2.114513 6.033521 7
GGTTC 2976740 2.0912273 5.8000474 3
TCGGA 2759680 2.080023 5.4784884 85-89
AGATC 2836650 2.0691543 8.087917 95-97
CTTTA 3351900 2.0556903 6.2010565 5
CTCCT 2013215 2.032626 8.520994 70-74
CTCTG 2407415 2.0275185 6.485308 20-24
ACATC 2318450 2.0273948 5.0121922 90-94
AGGAA 3062105 1.9989535 5.6510463 10-14
ATGGT 3503910 1.9871814 6.3259935 1
TATGC 2914865 1.9817847 5.7232804 30-34
GCTTC 2350285 1.9794035 6.854873 15-19
CCGCC 1327125 1.9741282 5.9752007 65-69
TTCCT 2588825 1.966732 7.0640483 45-49
GCTGC 2099830 1.9605113 5.53409 3
TTTCA 3189560 1.9561288 5.6112485 8
GGTGT 3324440 1.9481634 7.071717 9
ACCCA 1672765 1.9440182 27.24064 1
CTGTT 3044130 1.9290887 5.1419086 8
CCAAG 1939030 1.879734 5.171842 75-79
GCTCC 1673145 1.8727176 6.417941 70-74
CATGG 2480810 1.8698335 9.18406 2
CTTTC 2438830 1.8527805 6.53026 7
ACAAC 1971900 1.8500117 5.490506 85-89
GCACC 1530990 1.8384848 7.070698 3
GCTAC 2020715 1.8258619 13.908941 1
AAAGA 2886765 1.823777 5.327403 8
CCTTA 2232875 1.8199352 6.423464 95-97
TGCAG 2382550 1.795773 5.320062 20-24
GATCG 2357285 1.7767303 6.047948 95-97
CAGCT 1956710 1.7680287 5.1691537 65-69
ACTTT 2850845 1.7483977 7.415166 4
CAACA 1835155 1.7217193 5.7270465 2
TTCGG 2396350 1.6834903 7.1455193 7
CCTGT 1998280 1.6829458 6.5680327 1
CTACC 1552470 1.6816653 6.4052215 20-24
TACTT 2736970 1.6785592 6.989157 3
TGTTT 3518510 1.6777333 5.237788 10-14
TGCTG 2369465 1.6646029 5.5191073 6
ACCGC 1376000 1.6523657 5.644426 90-94
CTTGG 2348215 1.6496742 5.3367357 2
CCAGA 1700795 1.6487844 6.2390394 7
CGTGA 2161255 1.6289788 7.3725023 9
GTCTG 2316090 1.6271058 5.186168 90-94
GCTGG 2087825 1.6260198 6.3706107 4
CTCTA 1994465 1.625616 5.0440445 25-29
CACAC 1390535 1.6160222 5.6580486 95-97
GGAAA 2465520 1.6095005 5.1655316 9
TGGGA 2551735 1.6043218 5.6556635 15-19
ATGCC 1766490 1.5961512 7.0727363 9
TATTC 2595860 1.5920178 5.2690697 5
AGCCT 1752645 1.5836413 6.41503 2
CACCC 1087425 1.5654548 7.889614 4
TTCAT 2539525 1.5574679 5.377641 60-64
AGAAA 2431780 1.5363302 5.2131743 9
TTGGT 2893525 1.5295467 13.5690155 7
GCTTG 2165155 1.5210705 5.162024 1
GCTGT 2125140 1.4929593 5.541924 7
GGGAA 2204885 1.4872736 5.6892343 15-19
CAGCC 1236510 1.4848593 6.464427 20-24
TCGGT 2102345 1.476945 8.238307 8
CCCTA 1356980 1.4699067 5.8894353 25-29
GGAAT 2379305 1.447717 5.1350765 15-19
CCCAA 1244565 1.4463818 28.181505 2
GAACA 1841035 1.4407816 7.8297906 80-84
GCGAG 1721065 1.4380634 20.446585 1
TGGTA 2528825 1.434179 6.379881 2
TACTG 2108780 1.4337363 5.3113 3
CCAAT 1600420 1.3995051 20.464228 3
CTGTA 2053870 1.3964037 6.112152 8
CCTCC 1031795 1.3844765 5.2038956 2
ACACG 1405920 1.3629268 5.8743825 3
CATCC 1248015 1.3518738 5.4411707 90-94
ATGGG 2125295 1.3362113 7.5584135 3
ACGGT 1769740 1.3338865 6.751759 4
TGGAG 2079075 1.3071519 5.034066 3
AAGCC 1343960 1.3028616 5.7386484 1
TGGGT 2185475 1.2807156 6.271795 4
ATTGG 2242410 1.2717437 12.734927 6
GGCTG 1632325 1.2712716 22.033457 9
AACAC 1347890 1.2645733 8.337237 2
CCCAG 1050965 1.2620481 5.806976 6
AGCCC 1035500 1.2434771 7.0983686 25-29
GACGG 1473435 1.2311522 7.007005 3
CAGGA 1512750 1.2232792 5.0012736 4
ACATG 1674760 1.22163 8.974454 1
GTGTA 2153705 1.2214361 6.661965 2
TGGTC 1709035 1.2006359 15.263957 8
AGGTA 1931400 1.1751839 5.769252 35-39
GAGTC 1556475 1.1731447 17.927599 3
GCCCT 1029900 1.1527463 6.305841 25-29
AAGAC 1468700 1.1493948 6.308997 9
GCAAC 1183610 1.147415 7.25567 1
TCCTA 1395100 1.1370953 12.145499 8
GGTCG 1451335 1.1303147 7.358957 6
CGAAC 1123615 1.0892547 5.0080347 80-84
TACCG 1202285 1.0863513 14.246201 3
GACTG 1436765 1.082917 5.937211 1
CCTTC 1071520 1.0818514 5.5757804 45-49
GCCAG 1076990 1.0788124 6.3429365 2
CAATT 1626410 1.0701526 15.488483 4
CTGAC 1158820 1.0470775 8.6980715 1
AGACC 1079405 1.0463966 5.891384 2
GGGGA 1500930 1.0461348 5.2753015 8
ACCGT 1151245 1.040233 13.609797 4
GGGTC 1334675 1.0394586 7.208699 5
GGTAC 1371260 1.0335447 6.5588512 3
GTACT 1514475 1.0296749 5.9480233 4
TGGGG 1583640 1.0288088 5.152844 8
GTTCG 1461920 1.027032 5.520125 4
GGGCT 1316035 1.0249417 19.156391 8
GCCTA 1132325 1.0231375 5.525736 3
GTCCT 1203965 1.0139761 12.624943 7
TGACG 1312125 0.98897344 6.006635 2
CCTAG 1077585 0.97367585 13.050487 9
CCGCG 782285 0.9706777 5.4839277 90-94
GGTCA 1267870 0.9556176 16.12422 9
TGGCC 1016860 0.9493937 5.4490657 1
CGGTA 1248975 0.9413761 7.545675 5
TCGGG 1208460 0.94116116 19.008028 6
AATTG 1684050 0.9243091 12.056447 5
CCCCG 619260 0.9211631 7.1650877 1
CGTCC 802365 0.89807105 16.174135 6
TCGTG 1277390 0.8973955 6.55809 8
CGGGG 1032375 0.89133424 8.330502 6
ATTCG 1296770 0.8816597 5.6464734 6
ACCTC 805010 0.8720023 6.366543 4
AGGCA 1036100 0.83783805 6.211509 1
TGCCA 913245 0.82518286 5.8276243 1
CGAGT 1076860 0.81164974 18.020271 2
GTCGG 981945 0.764749 19.03897 5
CACGG 734760 0.73600334 8.613774 4
CCGTC 652305 0.7301119 16.330923 5
CGGGC 705095 0.7298006 24.851755 7
CAGAC 745370 0.7225765 6.354762 1
AGTCG 949800 0.71588224 18.239613 4
CCTCT 701045 0.7078043 6.0520444 5
ATACC 752465 0.65800154 13.052167 2
ACGGG 774195 0.64689106 5.243508 5
GGCAC 624480 0.6255367 5.5111 2
GTCGT 883195 0.6204646 6.5250587 7
CCACG 503955 0.6051729 5.338888 1
GACCT 640775 0.57898647 5.3203163 3
GATAC 792175 0.5778408 10.827409 1
CCCGG 464235 0.5760338 6.1795063 2
Produced by FastQC (version 0.10.1)