FastQCFastQC Report
Thu 31 Jul 2014
13L15A_CAGATC_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 13L15A_CAGATC_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 15165933
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 171055 1.1278897249513102 No Hit
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 167783 1.1063150549326572 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 119801 0.7899349153131561 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 108871 0.7178654949880103 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 94087 0.6203838563707225 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 78595 0.5182338600599119 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 78045 0.5146073110042093 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 60387 0.398175305139486 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 59607 0.393032199205944 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 58165 0.38352404695444714 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 57610 0.3798645292709654 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 49747 0.3280180652255288 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 46716 0.3080324830658292 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 44020 0.2902557989673303 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 42493 0.28018718004358845 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 40759 0.26875365992979133 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 38841 0.25610689431372274 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 36684 0.24188422828981243 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 36651 0.2416666353464703 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 35944 0.23700487137850337 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 33758 0.22259098731347424 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 32879 0.21679510254990578 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 30760 0.20282299809711674 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 30458 0.20083169297925818 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 28542 0.18819811481430124 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 27907 0.1840110990863536 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 27012 0.178109714713892 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 26172 0.17257098524700062 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 25448 0.16779712794458473 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 25034 0.165067325564474 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 22406 0.14773901480377105 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 21987 0.1449762437958812 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21292 0.1403936045345842 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21077 0.13897595354008224 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 20089 0.13246135269092907 No Hit
TGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 19729 0.13008761149083278 No Hit
AGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 19563 0.12899305304856615 No Hit
GGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 19205 0.1266324992995815 No Hit
TGACGAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 19151 0.12627643811956704 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 18883 0.1245093196706065 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 17826 0.11753975175810152 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 17823 0.11751997058143406 No Hit
TGGCTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 17495 0.11535722859912409 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 16495 0.10876350304330107 No Hit
TTGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 16419 0.10826237990105851 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 16408 0.10818984891994446 No Hit
GTCTGGAATTGTAATGCAAAGCGCCCACGCCGCCATGTTCCGGCAACGAG 15421 0.10168184179634712 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 16286005 7.3353906 12.636388 7
AGCAG 5887640 4.7466974 52.388474 20-24
TAGCA 6739015 4.127146 32.77448 20-24
CACGG 3836865 4.0995083 42.141247 45-49
GCAGA 4879195 3.9336746 34.42087 25-29
ACTTG 5723080 3.8086312 88.935165 8
CCTGC 3431875 3.6914313 43.328445 15-19
CTGCG 2972875 3.4515774 45.948063 15-19
TCACG 4112240 3.3376198 32.08583 45-49
GAAGT 5023080 3.3204818 26.286154 60-64
AGAGA 5395490 3.2822843 22.531675 90-94
GTTTC 4532720 3.2778096 32.072998 40-44
GAACC 4254565 3.177809 29.34038 70-74
CGGAT 3616465 3.1682558 32.123047 45-49
CTTGA 4528650 3.0137544 26.834223 55-59
GCAGC 2790310 2.9813137 18.16437 20-24
ACGGA 3693610 2.9778395 31.289274 45-49
AGTAG 4471470 2.9558427 25.751682 9
AGAGC 3629360 2.9260404 51.371754 95-97
AACTT 6232625 2.8995297 62.36371 7
CCATG 3404590 2.7632692 32.61932 75-79
GCGTT 2859295 2.7219534 35.14504 15-19
AACCA 5191265 2.710587 19.616049 70-74
TTCTC 4034690 2.7030709 25.577532 30-34
GAGCC 2529780 2.70295 61.096394 95-97
ACCTG 3314230 2.6899304 24.954151 15-19
CATGC 3279195 2.6614947 32.034958 75-79
CTTGT 3652070 2.6409724 96.83474 9
TGCGT 2773050 2.6398509 37.265957 15-19
ATAGC 4284565 2.6239774 23.931313 80-84
TGAAG 3963570 2.6200979 20.197987 60-64
GAGAG 3001525 2.6119797 41.555042 95-97
AGGAG 2959560 2.5754611 18.966467 90-94
AGAAG 4218065 2.566011 17.31365 6
ACCAT 4490435 2.5477934 27.086605 75-79
CACTG 3096345 2.5130882 31.520061 85-89
CTCAG 3089535 2.507561 32.12726 35-39
AGCAC 3355900 2.5065808 27.377907 80-84
TTCAC 4044705 2.4937239 19.774807 45-49
TTGAA 4941735 2.481496 19.095345 55-59
TGCAT 3689620 2.4553916 24.838478 75-79
AAGTT 4881435 2.4512165 20.760786 60-64
GGAAC 2979795 2.4023523 31.794945 70-74
TTTCA 4712760 2.3824158 19.460089 40-44
GCACT 2924225 2.3733902 23.811014 85-89
GCTGC 2042620 2.3715293 29.157879 1
GACTC 2871140 2.3303049 31.737791 25-29
AAACT 5363940 2.296438 34.085587 6
AAGGA 3743545 2.2773426 18.433357 70-74
GGATT 3141265 2.2564273 21.602081 50-54
ATGCA 3663315 2.243508 24.37008 75-79
GAAGA 3639580 2.2140965 8.707842 50-54
CAGAC 2964205 2.2140167 29.311436 25-29
CTGAA 3598310 2.2036974 23.729841 85-89
AGGAA 3618625 2.2013488 24.174313 70-74
ACAGG 2729150 2.2002785 12.433551 90-94
CAGCA 2911165 2.1744 14.49429 20-24
TTGTA 3963945 2.1629548 28.065329 9
GGAGG 1724605 2.146842 16.263649 90-94
GTAGT 2979015 2.1398802 21.582415 40-44
CAGTA 3481355 2.132071 24.08794 35-39
TCTCA 3439225 2.120421 18.766844 35-39
CATTC 3391850 2.0912123 24.63847 30-34
TTAGC 3141970 2.0909379 26.564068 20-24
TCAGT 3135055 2.0863361 26.51847 35-39
AGTTT 3812270 2.080192 21.97202 40-44
GATGA 3140375 2.075929 9.355041 15-19
GTTAC 3098370 2.0619226 24.462511 60-64
TAGAG 3112115 2.0572479 25.202583 90-94
CTGCA 2523085 2.0478127 16.452309 3
GTTAG 2831110 2.033637 28.590242 20-24
CATAG 3313985 2.0295694 24.041954 80-84
CAGAA 3593890 2.025501 12.688367 5
AACCT 3565585 2.02305 21.290567 10-14
GCATA 3296160 2.018653 18.315928 80-84
CTCAT 3271105 2.0167685 18.652191 30-34
AAGAG 3298275 2.0064676 9.595779 50-54
TAACC 3519780 1.997061 22.233965 10-14
ACCAG 2659275 1.9862593 11.955006 55-59
TGTAA 3910390 1.9636054 20.613903 10-14
TAAGG 2945590 1.9471674 24.338745 65-69
GTTGT 2493325 1.9461724 11.773249 35-39
GGAGC 1683735 1.9418093 18.421196 95-97
GTAAC 3127405 1.9153032 24.276701 10-14
CTAAG 3118100 1.9096044 23.89201 65-69
GGCTC 1640570 1.9047402 17.252754 65-69
GTAGG 1997565 1.8889242 17.835814 7
CGTTA 2830640 1.883752 20.137138 20-24
TGAAT 3749300 1.8827139 18.946968 85-89
CAGGC 1753740 1.8737879 13.847831 65-69
ACTGA 3055745 1.8714167 23.976095 85-89
TGTTG 2397055 1.8710285 10.648945 35-39
CCAGA 2504775 1.8708605 14.792811 55-59
GGAAG 2146290 1.8677394 8.66731 6
TCATT 3684175 1.862441 20.2023 30-34
AGACT 3029360 1.8552577 24.24071 25-29
AGGGA 2128110 1.8519187 16.592379 9
TTTCC 2758110 1.8478165 9.803258 40-44
TGCAG 2108550 1.8472254 18.74803 4
ATTCT 3643650 1.8419546 20.282652 30-34
GGGGG 1025550 1.8262064 8.144851 1
GAATA 3937335 1.8194945 13.907107 90-94
CCATC 2413630 1.814895 11.158861 75-79
ACTCA 3154555 1.7898388 21.12858 25-29
AATAG 3858775 1.7831911 18.315176 90-94
TTAAA 5048585 1.7722347 14.356331 50-54
CCAAA 3379385 1.7645249 28.525309 4
AGTTA 3502360 1.7587128 19.579462 60-64
TAGTT 3202085 1.7472404 21.584265 40-44
CCTCC 1729850 1.7238292 6.158109 1
TCCTC 2104050 1.7191849 8.467777 1
CAGAG 2107625 1.6991965 12.346196 25-29
GGATG 1785575 1.6884637 14.611229 10-14
TGATG 2339035 1.6801709 11.248669 30-34
TAGGG 1772555 1.6761514 17.867458 8
AAGAA 3910960 1.6632062 8.831526 45-49
CAGGA 2025990 1.6333811 11.491714 90-94
TTCCG 1851165 1.6326354 12.794248 40-44
TTGTT 2728130 1.617598 9.545695 35-39
ATTAA 4597840 1.614007 14.295212 50-54
TAAAC 3763915 1.611427 16.174423 50-54
AGCTG 1834280 1.6069472 7.0465803 1
TTACT 3176080 1.6055864 15.121723 65-69
GAGGA 1839365 1.6006478 16.736006 95-97
GATGT 2206695 1.5851084 13.289484 30-34
ATGAT 3150245 1.5818979 7.8919535 15-19
CATCA 2756455 1.5639639 7.7363515 80-84
GAGCT 1784030 1.562925 8.565791 95-97
ACCAA 2978860 1.5553932 14.638793 3
GAGAT 2340590 1.5472353 12.392242 25-29
GCTCA 1883645 1.5288237 14.950521 65-69
TGACC 1882140 1.5276023 41.673515 1
TGCTG 1600455 1.5235796 6.181664 1
AGGCT 1736585 1.52136 12.672763 65-69
GAGCG 1317210 1.5191053 7.2037406 90-94
GATTA 3021055 1.5170251 19.763645 50-54
TGTTT 2543475 1.50811 11.306081 35-39
ATAGA 3250745 1.5022122 18.13603 90-94
AACAG 2661770 1.5001622 12.665562 60-64
TACTA 3223090 1.4994395 18.322231 65-69
GAGAA 2426570 1.4761759 11.660907 50-54
AAAGA 3423305 1.455822 7.112911 45-49
ACTAA 3399625 1.4554652 17.068375 65-69
TCCGT 1636570 1.4433733 13.167021 40-44
GGGAT 1525290 1.4423345 17.260002 10-14
CAAAC 2759910 1.4410698 33.31795 5
GAAAC 2549225 1.4367323 8.440081 60-64
AGAAA 3351920 1.4254643 6.208025 60-64
AGCCA 1908330 1.4253652 12.734475 95-97
CTCAC 1887190 1.4190459 10.792861 65-69
CTACA 2489065 1.4122515 8.951174 85-89
TACAG 2296870 1.4066621 8.844791 90-94
TACCA 2413985 1.3696524 8.633168 75-79
GACCA 1809370 1.3514503 18.523209 2
ATACC 2365095 1.3419131 8.228846 75-79
GCTCC 1245365 1.3395532 5.790997 1
CAACA 2553305 1.3331923 5.953767 8
TAAAG 2878885 1.3303709 7.596366 45-49
GGCCA 1243415 1.32853 17.325384 2
ATGTT 2431820 1.3269398 8.120235 30-34
CGGAA 1629945 1.3140843 7.349374 5
CTCTT 1959905 1.313053 5.858662 3
AGATG 1983825 1.3113976 9.700247 25-29
GCCAA 1734350 1.2954165 14.269355 3
TCTTG 1789710 1.2942181 5.288892 4
GCTGG 1023335 1.2824379 6.5833845 1
GTAAA 2771910 1.2809364 6.8381996 45-49
AGCGT 1445040 1.2659479 5.197251 90-94
AAGTA 2667190 1.2325438 18.949005 8
TGGCC 1059490 1.2300926 28.909428 1
CCTCT 1501995 1.2272556 6.842702 2
AGAAC 2166240 1.2208835 9.049263 50-54
AAACA 3090015 1.2174348 6.4474826 60-64
TCTAC 1959850 1.2083266 10.699021 85-89
TCAAT 2576920 1.1988297 6.9165754 80-84
CAATA 2786325 1.192896 7.8249493 80-84
GCCCA 1202805 1.1906192 7.2677145 2
GATAC 1936395 1.185898 7.700524 75-79
ATCAA 2747655 1.1763405 6.4113774 80-84
CCGTA 1441685 1.1701156 14.659911 40-44
CCCAA 1605730 1.1111369 7.5026445 3
TGATA 2188020 1.098716 7.950157 15-19
GATAG 1661020 1.0980089 12.129059 15-19
TCAAC 1903500 1.0800122 6.615462 7
CGTAA 1678695 1.0280759 8.670726 40-44
CCGAA 1371175 1.0241548 28.145838 4
GGATA 1543570 1.0203693 9.250954 70-74
TATCT 2006585 1.014378 7.150097 85-89
TTGAC 1505085 1.0016134 5.13658 6
GGTGC 796005 0.9975493 8.1784 2
GTTGC 1031270 0.9817345 5.4961467 1
CTCCG 911660 0.9806099 5.64241 2
ATCTA 2106525 0.97999334 6.8585906 85-89
GTCCA 1204690 0.9777633 5.2660193 2
GTGCA 1106135 0.9690453 6.4615507 3
AATAT 2746005 0.9639464 5.115582 80-84
GAACT 1551975 0.9504693 23.238285 6
GGGGT 698090 0.9442947 6.2533827 1
GGGTG 697735 0.9438146 9.852344 1
TCCCC 941525 0.93824804 5.282387 1
GGCTG 736105 0.92248297 8.342645 1
GGCCT 785905 0.9124541 6.142736 2
CTAAA 2130080 0.911941 5.677078 4
TGTCC 1029865 0.90828973 8.397074 1
GACCT 1094265 0.8881389 9.025829 2
GCTGT 925090 0.88065463 6.816367 1
TGGGG 649855 0.87904805 5.1158667 1
CGAAC 1176840 0.8790024 26.340294 5
TGCCC 813635 0.8751712 7.616245 1
ATATC 1872625 0.87117887 5.910009 85-89
CTGTC 975660 0.8604835 7.7977686 4
CCCCC 703915 0.8555105 5.6008534 1
AAGTC 1387630 0.8498203 6.15616 6
CCTAA 1495285 0.8483982 8.880199 4
TGGCT 883315 0.84088624 6.244487 1
TGTCA 1251790 0.83304906 6.4258547 5
GTCTG 872695 0.8307764 5.1205907 1
GTCAA 1339210 0.82016665 6.914243 6
ACCTA 1445100 0.8199242 6.370823 3
GGGGC 487785 0.8047183 5.464424 1
GGGCC 521460 0.79700106 9.883264 1
ACCGA 1043760 0.7796027 16.731775 3
CCGTC 710270 0.7639885 5.7920165 4
AGTCT 1131335 0.7528879 7.810963 7
CCCCG 537225 0.7047561 6.3646984 2
GGCCG 460995 0.7045861 17.07464 2
TCTAA 1476070 0.6866943 5.841535 9
TGCGG 537955 0.6741624 10.76623 3
GCCCC 512650 0.6725175 6.4656286 1
GGCCC 472710 0.66935277 8.098904 1
GCCGA 608335 0.6499771 12.30706 3
GACCG 607085 0.6486415 23.448002 2
GTCCC 599075 0.6443837 5.269051 1
TGACT 960160 0.63897336 10.237695 1
CTAAC 1121690 0.6364271 7.9030986 5
GACTG 715305 0.62665313 6.9507008 2
TCCCG 580765 0.6246889 5.056355 2
TAACT 1309435 0.60917276 6.7381973 6
GACTT 915030 0.6089399 7.241503 6
CCCGA 613560 0.6073439 5.7761045 3
GACCC 596555 0.5905112 5.414887 2
GTCTA 876200 0.58309907 7.8164506 8
GCGGA 493140 0.56872606 9.295862 4
GCCCG 395150 0.5595285 6.3966374 2
CCCGT 496420 0.53396475 6.2066154 3
GTCCG 457590 0.5312727 6.1821666 2
Produced by FastQC (version 0.10.1)