FastQCFastQC Report
Thu 31 Jul 2014
13L15A_CAGATC_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 13L15A_CAGATC_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 15165933
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 253602 1.6721819884078348 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 157651 1.0395074276010583 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 117930 0.7775980547982112 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 101564 0.6696851423516113 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 73510 0.48470476560855175 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 70785 0.466736863468934 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 69659 0.4593123284930772 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 62189 0.41005719859107914 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 59891 0.3949048172637978 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 58879 0.38823196700130486 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 55626 0.3667825777682125 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 47109 0.3106238172092676 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 45617 0.30078597867997964 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 42632 0.2811037078958479 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 41991 0.2768771298145653 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 41516 0.27374511017554937 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 41334 0.27254505212438956 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 40232 0.26527876656187255 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 39902 0.26310283712845095 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 38987 0.2570695782448729 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 38646 0.25482111783033723 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 37457 0.24698117814446366 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 36295 0.2393192690485973 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 33188 0.2188325637466551 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 32969 0.21738853784992984 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 32742 0.215891762148758 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 31280 0.20625173538614475 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 28837 0.19014326385326902 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 28449 0.1875848983376097 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 28201 0.18594965439976557 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 27832 0.1835165696696669 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 27350 0.18033839395176018 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 26194 0.17271604720922873 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 26165 0.17252482916810988 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 25872 0.17059286758025372 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 25594 0.1687598118757349 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 25478 0.16799493971125942 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 25347 0.1671311616634466 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 24254 0.15992421963093204 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 24020 0.15838128785086944 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 23440 0.15455692702849208 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 22993 0.15160953170503919 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 22577 0.1488665418738168 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 21969 0.14485755673587639 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 21898 0.14438940222141294 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 21530 0.14196291121687007 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 21109 0.13918695275786858 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 20445 0.13480871898880206 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 20267 0.13363503583986555 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 20032 0.13208551033424717 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 19960 0.1316107620942279 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 19178 0.12645446870957427 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 18711 0.12337519887500492 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 18566 0.12241910866941058 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 17752 0.11705181606697063 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 17320 0.11420332662685508 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 16920 0.11156583640452586 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 16590 0.10938990697110425 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 16052 0.10584248262207145 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 6010355 3.6620088 5.2454457 90-94
CTGCT 4264295 3.2185326 9.217895 4
ACTTC 4327420 3.0490477 6.2094045 60-64
AACTT 4994040 2.8268468 5.475075 60-64
AGCTG 3659595 2.7514472 6.724863 3
CTACT 3662115 2.5802817 9.097566 2
GCTGC 2752340 2.575818 9.031313 3
GGAAG 3431830 2.5702267 5.7608223 15-19
CTTCT 4215300 2.5658817 5.5151777 20-24
TTCAA 4465270 2.5275393 6.9086337 55-59
CTTCA 3571175 2.5162067 6.203179 60-64
CAACT 3008620 2.4537456 7.6922765 9
TGCTA 3921470 2.377796 5.613443 1
TCTCT 3877475 2.3602455 7.650544 20-24
CAGCA 2318720 2.3448384 7.058365 2
CTGGT 3607730 2.3433392 10.00676 1
CAAGC 2314960 2.3410363 7.738808 2
TCAAC 2836815 2.313626 7.01668 8
ACTGC 2561230 2.2376192 5.8064723 2
TGGTG 3995990 2.2336545 7.0435853 6
GGTGG 3218935 2.2310343 7.1113 7
CTGAA 3169235 2.2243705 5.5565734 80-84
GCAGC 2046540 2.2169726 7.199201 1
CGGTG 2728320 2.197348 11.641804 9
AGGAA 3112690 2.1762364 5.6841693 10-14
GGTGT 3890005 2.1744115 10.015256 1
ACAAC 2290470 2.1622894 6.819973 85-89
GAAGC 2479245 2.157618 6.4003563 15-19
ACCCA 1812790 2.130206 36.213024 1
CAACA 2239120 2.1138132 6.62626 85-89
GCTAC 2415475 2.1102805 11.624412 1
AAGCT 2988465 2.0974946 5.1003647 15-19
ACATC 2559025 2.0870686 5.62312 90-94
CAGCT 2376020 2.0758107 6.028398 2
CATGG 2746270 2.0647683 15.678173 2
TCCTT 3362730 2.0469167 6.8143606 40-44
CAAGG 2331295 2.0288615 6.0208783 10-14
TATGC 3324745 2.0159698 6.6495357 30-34
CCAAG 1983870 2.0062165 6.6766763 75-79
GCTCC 1843115 2.0043569 6.531414 65-69
CCAGC 1569120 1.9751757 7.9933014 1
CTCCT 2248525 1.9720498 7.8435745 70-74
GCTGG 2432885 1.9594092 7.8553257 4
CTTGG 3007790 1.9536585 6.452422 2
GCTTC 2554840 1.9282987 7.816573 15-19
AGCTC 2152990 1.8809603 5.589684 65-69
CTTTA 3842330 1.8789614 5.211217 5
TTTCA 3802965 1.8597112 5.9378133 8
CGTGA 2451220 1.8429369 13.202464 9
CCGTG 1960415 1.8346832 5.1278324 60-64
TGGCT 2798725 1.8178641 5.0164123 9
CTCTG 2397010 1.8091744 6.5543637 20-24
TTCCA 2537900 1.7881736 5.1249943 75-79
AAGGA 2556080 1.7870827 5.5311894 10-14
CACAA 1835445 1.7327287 6.0809145 85-89
TATTC 3508785 1.7158526 6.4309998 5
CCTTA 2424540 1.7083015 7.0722003 95-97
CACCC 1148310 1.679648 5.9805646 50-54
CTTTC 2751330 1.6747534 7.2371235 7
CCCAA 1422815 1.6719472 36.615677 2
CCAAT 2031035 1.6564548 25.5271 3
CCGCC 1055740 1.6542134 6.3783326 45-49
TTCGG 2542270 1.6512879 9.30178 7
CCTGT 2184060 1.6484479 6.16636 4
CATCC 1616500 1.6410539 6.453728 90-94
GTGGC 1999010 1.6099728 5.727419 8
GCACC 1268235 1.5964279 5.2983246 50-54
TCGGT 2457550 1.5962595 10.781551 8
CCACA 1355460 1.5927986 8.216717 1
TTGGT 3461120 1.5602918 15.181672 7
AGAAA 2367615 1.5452751 5.6923656 9
AATGA 2707210 1.5264748 5.0793247 75-79
GCTTG 2344000 1.522505 6.427879 1
GCAAG 1740070 1.5143347 5.450748 1
GAACA 1853935 1.5061707 5.741343 80-84
TGTAG 2849610 1.4869678 5.330626 9
TCCAA 1823155 1.4869137 5.3432913 75-79
GCTGA 1968620 1.4800966 5.0290203 80-84
TGAAC 2099520 1.4735767 5.251271 80-84
CATGC 1684135 1.471345 6.0089154 2
TACTT 2986685 1.4605373 7.258945 3
ACATG 2079305 1.4593883 15.4239645 1
GCAAC 1430305 1.4464161 6.2754803 1
CTACC 1418975 1.4405285 5.272789 25-29
ACACA 1512045 1.4274272 5.829069 85-89
GTGTA 2724530 1.4216993 9.121519 2
GCGGC 1215575 1.4105788 5.7857246 7
TGGTA 2689635 1.4034905 7.661428 2
CTGTA 2309880 1.400603 6.348851 8
TTAGG 2649195 1.3823884 5.125148 35-39
ATGGG 2120590 1.3720671 13.543781 3
ACTTT 2803335 1.3708761 7.5884423 4
CGCAG 1254505 1.3589782 5.016368 90-94
GTAGC 1805045 1.3571136 5.496998 1
AACAC 1434365 1.3540941 5.846188 85-89
AGCCT 1546585 1.3511745 5.889682 4
ACGGT 1789335 1.3453021 6.4548364 4
ATGCC 1535395 1.3413984 6.876033 9
GGTCG 1650280 1.329111 13.829584 6
AAGCC 1305025 1.3197252 6.6377463 2
GTATT 3054090 1.2852736 6.1112566 4
CAATT 2255845 1.2769077 17.848719 4
TGGGT 2273430 1.2707882 10.276671 4
GGTAC 1627575 1.2236837 9.089298 3
GGGTC 1510960 1.2169046 13.799327 5
GACGG 1288205 1.2009242 8.173528 3
TGGCC 1276675 1.1947951 9.6257715 1
GCCTG 1275645 1.1938311 6.949723 6
TGGTC 1827090 1.1867551 17.850136 8
GGTCA 1552090 1.1669307 20.455494 9
CACAT 1409245 1.1493404 5.410586 2
TGTAT 2726315 1.1473336 6.0153666 3
GCTCA 1306100 1.1410747 5.9653087 1
TGGGG 1601210 1.1097939 5.4213986 9
GTACT 1817655 1.1021409 7.294272 4
TCGTG 1692855 1.0995649 11.269561 8
CTGAC 1254750 1.0962127 7.593328 1
ATTCG 1791780 1.0864514 7.8225393 6
ATTGG 2041695 1.065386 15.005467 6
TGACG 1402480 1.0544472 6.4650235 2
GGGGT 1506250 1.0439775 5.3847933 8
AATTG 2030215 0.988969 13.577054 5
GGCCA 875995 0.9489464 5.0104003 1
GCCCA 716890 0.9024063 5.3821464 2
GGCCT 950540 0.88957685 6.6724563 5
GCCTA 988540 0.8636382 5.1269903 3
CGGTA 1132385 0.85137767 6.049587 5
CTACA 988200 0.805948 6.856457 3
CCCCG 501835 0.7863131 6.3265343 1
GGCCC 582135 0.78496224 6.3942733 1
GTCGT 1204320 0.7822455 11.061765 7
CCCGG 422360 0.56951857 5.1921473 2
Produced by FastQC (version 0.10.1)