FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | Undetermined_Undetermined_L006_R2_001.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 100925331 |
| Filtered Sequences | 0 |
| Sequence length | 101 |
| %GC | 43 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content
Per base GC content
![[WARN]](Icons/warning.png)
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | 199476 | 0.19764711002037733 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| TGACC | 26419450 | 3.2418346 | 178.11005 | 1 |
| CAGTG | 24761495 | 3.0697157 | 187.71373 | 2 |
| GCTTT | 25871135 | 2.9461267 | 150.9761 | 3 |
| CGATG | 23708535 | 2.9391787 | 177.42957 | 1 |
| GACCA | 26244770 | 2.8896773 | 161.70708 | 2 |
| AAGAA | 44496080 | 2.735421 | 5.370185 | 9 |
| TGGTG | 19395315 | 2.7072773 | 6.801635 | 7 |
| CTCAG | 20846550 | 2.5580041 | 187.64311 | 1 |
| TGTTG | 22187155 | 2.5526528 | 37.479603 | 4 |
| TCAGA | 27352700 | 2.5079288 | 139.65166 | 2 |
| CAGAT | 26367470 | 2.4175944 | 141.50386 | 3 |
| CTTTG | 21189450 | 2.4129903 | 40.23279 | 4 |
| ACAGT | 25883295 | 2.3732011 | 142.19025 | 1 |
| GATGT | 22798635 | 2.3536315 | 134.31598 | 2 |
| ACCAT | 25931055 | 2.35332 | 134.79927 | 3 |
| TGCTG | 16745660 | 2.3135774 | 8.318487 | 7 |
| AGTGT | 22331095 | 2.3053646 | 162.2338 | 3 |
| CAAGA | 27706160 | 2.279454 | 5.533038 | 8 |
| AGCTT | 22247070 | 2.2732556 | 132.11409 | 2 |
| TGAAG | 24097105 | 2.2322047 | 8.192987 | 7 |
| GATCA | 23628910 | 2.1664999 | 112.225815 | 1 |
| TTGGT | 18593275 | 2.1391735 | 5.742917 | 6 |
| TTTGG | 18316795 | 2.1073642 | 10.768336 | 5 |
| ATGTT | 24267480 | 2.064945 | 110.610245 | 3 |
| ATCAG | 22092255 | 2.0256064 | 111.94866 | 2 |
| GTGTC | 14389730 | 1.9880823 | 74.67898 | 4 |
| CCATC | 16354440 | 1.9863169 | 61.9723 | 4 |
| TCAAG | 21634385 | 1.9836249 | 13.787529 | 7 |
| TAGCT | 19391410 | 1.9814578 | 132.27246 | 1 |
| TTGAA | 25386610 | 1.9383316 | 7.5603843 | 6 |
| TTCCA | 19106780 | 1.9324523 | 5.004672 | 6 |
| TCAGT | 18758365 | 1.9167719 | 124.8548 | 3 |
| CAACA | 23250390 | 1.8933485 | 5.0109816 | 8 |
| TGCCA | 15252585 | 1.8715892 | 10.849487 | 7 |
| TCCAA | 20615675 | 1.8709337 | 6.285258 | 7 |
| GTTGC | 13448120 | 1.8579897 | 11.743615 | 5 |
| GTGGT | 13090340 | 1.8272029 | 7.527871 | 6 |
| CTTTC | 16017795 | 1.8054458 | 53.26202 | 4 |
| TGGTT | 15631225 | 1.7983869 | 5.037378 | 7 |
| TGGCC | 10727350 | 1.7797778 | 5.269875 | 7 |
| TTGCC | 12967815 | 1.7733495 | 7.060593 | 6 |
| TGTTC | 15286815 | 1.7408162 | 50.853756 | 4 |
| AGATC | 18881930 | 1.7312564 | 49.50476 | 4 |
| TTCTT | 18433445 | 1.7302012 | 7.384216 | 6 |
| ATGGT | 16721935 | 1.7262995 | 14.233323 | 3 |
| GTTGA | 16357570 | 1.6886841 | 10.539224 | 5 |
| TTGGC | 12156345 | 1.6795183 | 5.4491844 | 6 |
| GTCAA | 18269485 | 1.6751021 | 19.197924 | 6 |
| GTTGG | 11995635 | 1.6743997 | 10.743874 | 5 |
| TGTCG | 12077440 | 1.6686169 | 17.537298 | 5 |
| TTGCT | 14601310 | 1.6627531 | 6.715682 | 6 |
| TGGTC | 11889715 | 1.6426808 | 6.624097 | 4 |
| CCATG | 13353300 | 1.6385348 | 47.427864 | 4 |
| TTTGA | 19184100 | 1.6323948 | 9.438898 | 5 |
| TCAAA | 23860320 | 1.6180241 | 15.839358 | 7 |
| GTTCT | 14035335 | 1.5983015 | 12.781206 | 5 |
| TTCTG | 13945580 | 1.5880805 | 5.758421 | 6 |
| TTGTT | 16742735 | 1.5877085 | 6.112738 | 6 |
| CAAAA | 26027245 | 1.5837128 | 5.670093 | 8 |
| TGTCT | 13826150 | 1.5744802 | 15.996114 | 5 |
| GTGTT | 13638235 | 1.5690916 | 23.773767 | 4 |
| TTCAA | 20731775 | 1.5667715 | 14.681015 | 6 |
| TCTTC | 13822215 | 1.557971 | 9.009939 | 7 |
| CCATT | 15320315 | 1.5494907 | 20.436655 | 4 |
| TTTTG | 16300335 | 1.5457556 | 7.752784 | 5 |
| ATCAA | 22595210 | 1.5322341 | 15.44457 | 6 |
| TGCTT | 13381385 | 1.5238317 | 5.3621793 | 7 |
| TCAAC | 16781270 | 1.52295 | 11.588974 | 7 |
| AGATG | 16333865 | 1.5130669 | 35.752445 | 4 |
| GATGA | 16324135 | 1.5121655 | 11.205836 | 5 |
| TTTCT | 15978920 | 1.4998143 | 12.414132 | 5 |
| TGGCT | 10816010 | 1.4943379 | 7.5823703 | 7 |
| TGTCC | 10902315 | 1.4908923 | 10.415979 | 5 |
| TGGAA | 15793590 | 1.4630191 | 7.778444 | 7 |
| CATTG | 14241900 | 1.4552693 | 8.781849 | 5 |
| ATGTC | 14234050 | 1.4544673 | 5.160859 | 6 |
| TTGGA | 13937880 | 1.4388858 | 5.829828 | 6 |
| GTGCT | 10396260 | 1.4363453 | 9.153478 | 6 |
| TCTTT | 15298030 | 1.4359045 | 8.236224 | 7 |
| GTCGC | 8622510 | 1.4305631 | 10.937722 | 6 |
| GATGG | 11313850 | 1.4170521 | 12.079972 | 5 |
| TCTGC | 10329760 | 1.4125953 | 5.2824516 | 7 |
| CATCA | 15532960 | 1.409662 | 19.465328 | 5 |
| TGTCA | 13636860 | 1.393445 | 23.319714 | 5 |
| TCCCA | 11409705 | 1.3857577 | 6.095592 | 7 |
| CTTTT | 14668990 | 1.3768616 | 16.957943 | 4 |
| TGTTT | 14482130 | 1.373336 | 16.421944 | 4 |
| TGAAA | 20005365 | 1.3705957 | 9.350331 | 7 |
| TTGTC | 12000725 | 1.3666062 | 5.029928 | 6 |
| GTTGT | 11833825 | 1.3614925 | 8.398963 | 5 |
| TTTCA | 15978025 | 1.3457139 | 17.270292 | 5 |
| GTTCC | 9788435 | 1.3385692 | 8.570578 | 5 |
| TTTCC | 11861950 | 1.3370197 | 8.004644 | 5 |
| GTTTG | 11601295 | 1.3347397 | 8.321741 | 5 |
| GTCAC | 10862195 | 1.3328604 | 10.1459 | 6 |
| TCTTG | 11679260 | 1.3299987 | 5.3194947 | 7 |
| CATGG | 10663270 | 1.3219398 | 11.465214 | 5 |
| TTTGC | 11573005 | 1.3178988 | 10.635375 | 5 |
| CATCT | 12837205 | 1.2983499 | 13.725153 | 5 |
| GATTG | 12532140 | 1.2937634 | 9.285824 | 5 |
| TGTGG | 9177230 | 1.2809952 | 14.071291 | 5 |
| TCGGT | 9253990 | 1.2785295 | 6.6990047 | 7 |
| TCTGG | 9208525 | 1.272248 | 5.36116 | 7 |
| TCGCT | 9237285 | 1.2631992 | 9.405355 | 7 |
| TTGCA | 12280560 | 1.2548553 | 5.15645 | 6 |
| TTCTC | 11128510 | 1.2543502 | 7.3651013 | 6 |
| AGTTG | 12097120 | 1.2488538 | 7.306168 | 5 |
| TGCAA | 13480670 | 1.2360228 | 6.577816 | 7 |
| GATCG | 9962125 | 1.235018 | 14.694429 | 5 |
| AGATT | 16119025 | 1.230728 | 15.791097 | 4 |
| ATGGC | 9913695 | 1.229014 | 5.503867 | 6 |
| TGAAC | 13399730 | 1.2286015 | 5.2586694 | 7 |
| TCTGA | 12016150 | 1.2278372 | 5.092735 | 7 |
| TGCTC | 8967275 | 1.2262754 | 5.129742 | 7 |
| GCCAT | 9976180 | 1.2241408 | 5.6939373 | 8 |
| TCTCT | 10805010 | 1.2178868 | 5.905879 | 7 |
| GTCCA | 9915565 | 1.2167029 | 6.175087 | 6 |
| GTGTG | 8697760 | 1.2140689 | 56.50998 | 4 |
| CCCAG | 8223390 | 1.2117404 | 6.029328 | 1 |
| TGTTA | 14160755 | 1.2049533 | 30.903782 | 4 |
| CAGTA | 13124560 | 1.2033715 | 30.22025 | 4 |
| ATGAA | 17277225 | 1.183687 | 7.9534287 | 6 |
| CAGTC | 9581360 | 1.1756938 | 50.58326 | 4 |
| TCTCC | 8636600 | 1.1690044 | 5.9605513 | 7 |
| CTTTA | 13757495 | 1.1586946 | 30.218401 | 4 |
| GTCGT | 8379810 | 1.157753 | 7.286793 | 6 |
| ATCTC | 11426600 | 1.1556818 | 7.4157166 | 6 |
| TTTGT | 12155295 | 1.1526828 | 8.534418 | 5 |
| GTGCC | 6929640 | 1.1496986 | 9.699617 | 6 |
| GATGC | 9257000 | 1.1476027 | 11.71448 | 5 |
| CGCTT | 8386100 | 1.1467998 | 5.9098105 | 2 |
| GTCGA | 9241210 | 1.145645 | 9.98275 | 6 |
| GCCTT | 8258265 | 1.1293184 | 5.249904 | 3 |
| ATCCA | 12383650 | 1.1238528 | 5.170463 | 6 |
| GTTCA | 10997255 | 1.1237242 | 18.853516 | 5 |
| TTATT | 15838855 | 1.1108664 | 5.066848 | 6 |
| TCGCC | 6669485 | 1.0952455 | 7.5508714 | 7 |
| TCGTT | 9503660 | 1.082248 | 5.68631 | 7 |
| GTGGA | 8622415 | 1.0799518 | 7.9682226 | 6 |
| GTGGC | 6438705 | 1.0792599 | 7.4489675 | 6 |
| ATGCT | 10430575 | 1.0658196 | 6.6617994 | 6 |
| GTGAA | 11380810 | 1.0542469 | 10.44011 | 6 |
| TCACT | 10408440 | 1.0527055 | 5.779047 | 7 |
| GTCTC | 7677455 | 1.0498925 | 9.854527 | 6 |
| ATCGG | 8467595 | 1.0497391 | 5.3536453 | 6 |
| TCCGT | 7646090 | 1.0456033 | 5.1143913 | 3 |
| CAGTT | 10196030 | 1.0418533 | 16.262608 | 4 |
| AAAGT | 15190430 | 1.0407177 | 8.914279 | 1 |
| AGTTT | 12227325 | 1.0404358 | 8.868363 | 3 |
| ATGGA | 11201130 | 1.0376024 | 5.9677277 | 6 |
| CATCG | 8380870 | 1.028386 | 13.3833 | 5 |
| GTCTT | 9026005 | 1.0278542 | 9.104418 | 6 |
| ATCTG | 10021870 | 1.0240573 | 5.972892 | 6 |
| GTCTG | 7403365 | 1.0228475 | 7.3721423 | 6 |
| GTCAT | 9939055 | 1.015595 | 6.173609 | 6 |
| CATCC | 8320040 | 1.0105046 | 8.570502 | 5 |
| TCTCA | 9881940 | 0.9994556 | 6.945116 | 7 |
| TCACA | 10945445 | 0.9933315 | 8.239804 | 7 |
| GTTAC | 9704910 | 0.99166954 | 6.220001 | 5 |
| GTGTA | 9592340 | 0.9902713 | 43.777637 | 4 |
| TATTT | 13968010 | 0.97965366 | 5.3411274 | 7 |
| ATCTT | 11577630 | 0.9751003 | 7.7984543 | 6 |
| TTCAG | 9515230 | 0.9722876 | 7.144936 | 6 |
| TAAGA | 14174945 | 0.97114533 | 6.2026362 | 7 |
| AGTCA | 10569825 | 0.9691317 | 15.28481 | 5 |
| TTTAT | 13705435 | 0.9612378 | 5.6559577 | 5 |
| TTTAC | 11402155 | 0.96032125 | 5.438348 | 5 |
| ATGCC | 7782565 | 0.95497024 | 7.4098186 | 6 |
| CATGA | 10410965 | 0.95456606 | 10.594365 | 5 |
| TTCAC | 9411670 | 0.9518926 | 7.8739147 | 6 |
| TCAAT | 12583495 | 0.950978 | 9.232019 | 7 |
| TCAGC | 7683970 | 0.942872 | 8.559115 | 7 |
| AGATA | 13681175 | 0.93731654 | 28.754644 | 4 |
| TCGAA | 10211920 | 0.9363159 | 8.781966 | 7 |
| TAGAA | 13380285 | 0.9167021 | 9.943952 | 7 |
| GTCCC | 5538300 | 0.90948534 | 5.8700037 | 6 |
| ATATT | 14329320 | 0.90178514 | 5.294715 | 6 |
| TTTCG | 7916715 | 0.9015315 | 12.295211 | 5 |
| GATCT | 8809885 | 0.90021384 | 14.09078 | 5 |
| CCATA | 9901915 | 0.89862806 | 35.900616 | 4 |
| GTAGA | 9675390 | 0.8962675 | 9.484986 | 6 |
| GTCAG | 7214190 | 0.8943528 | 9.801991 | 6 |
| TGGGA | 7100605 | 0.88934606 | 5.240731 | 7 |
| GTTAT | 10444985 | 0.88877463 | 6.112788 | 5 |
| TCTCG | 6480505 | 0.8862094 | 6.3662086 | 7 |
| GTAAG | 9542770 | 0.88398236 | 5.8520656 | 6 |
| ATCGA | 9638035 | 0.8836972 | 7.299382 | 6 |
| TATTC | 10457885 | 0.8807923 | 5.9366083 | 7 |
| TTTAG | 10324820 | 0.8785496 | 9.063952 | 5 |
| TTCGT | 7635565 | 0.86951506 | 5.4702373 | 6 |
| CATGC | 7077125 | 0.86840826 | 12.102259 | 5 |
| GTCGG | 5109545 | 0.8564653 | 7.3980885 | 6 |
| GTTCG | 6185510 | 0.8545889 | 14.031433 | 5 |
| TTCGG | 6111770 | 0.84440106 | 5.310041 | 6 |
| TGTGC | 6088515 | 0.8411881 | 14.903409 | 5 |
| ATGCA | 9170675 | 0.8408457 | 5.1814847 | 6 |
| TTTAA | 13114605 | 0.8253397 | 8.940947 | 5 |
| GTGGG | 4871755 | 0.82502496 | 5.9864955 | 6 |
| GTAAC | 8840355 | 0.81055915 | 5.7159276 | 6 |
| TAAAG | 11603230 | 0.7949535 | 5.182794 | 7 |
| TTCGC | 5793880 | 0.79231346 | 7.879975 | 6 |
| TCGAG | 6388375 | 0.7919753 | 5.015033 | 7 |
| ATCAC | 8711960 | 0.7906362 | 8.398835 | 6 |
| TTCGA | 7730040 | 0.7898729 | 7.2357073 | 6 |
| CATGT | 7725240 | 0.7893824 | 9.653819 | 5 |
| GTTAA | 10296770 | 0.7861843 | 10.633393 | 5 |
| ATCGT | 7626160 | 0.7792582 | 5.5894446 | 6 |
| GATAT | 10172855 | 0.776723 | 6.5756273 | 5 |
| AGTCT | 7579400 | 0.77448016 | 10.332277 | 5 |
| TTAGA | 10134160 | 0.77376866 | 7.2454314 | 6 |
| CGACG | 5157425 | 0.7677959 | 5.4226832 | 1 |
| TCGAT | 7503795 | 0.7667546 | 5.197082 | 7 |
| GATCC | 6196530 | 0.7603536 | 9.243943 | 5 |
| TAAAA | 14996060 | 0.7598602 | 8.556302 | 7 |
| GATAA | 11052905 | 0.7572501 | 11.153832 | 5 |
| GTCTA | 7391060 | 0.75523514 | 5.9670453 | 6 |
| GTATT | 8860905 | 0.7539836 | 6.3999243 | 6 |
| AGTCC | 6101675 | 0.7487143 | 6.762257 | 5 |
| TGTAG | 7241115 | 0.74754107 | 12.808862 | 5 |
| ATCGC | 6040300 | 0.7411832 | 7.6982126 | 6 |
| AGTGG | 5916415 | 0.74102706 | 10.052288 | 5 |
| AAGTG | 7907080 | 0.73246235 | 5.1545105 | 2 |
| TTAAA | 12952515 | 0.7314273 | 6.897596 | 6 |
| GTGAT | 7030235 | 0.7257708 | 5.9043646 | 6 |
| TGTGT | 6175515 | 0.71049875 | 10.345795 | 5 |
| AGTGC | 5526840 | 0.68516976 | 10.0865555 | 5 |
| CATAT | 9043255 | 0.68342984 | 6.46008 | 5 |
| AGTCG | 5504225 | 0.68236613 | 11.849093 | 5 |
| GTTAG | 6543655 | 0.67553836 | 10.667192 | 5 |
| TCGCA | 5427690 | 0.6660121 | 6.4453974 | 7 |
| ATAAA | 13123345 | 0.66496855 | 6.970068 | 6 |
| GATAC | 7240975 | 0.6639143 | 6.4273286 | 5 |
| TCGAC | 5392795 | 0.66173023 | 5.4893603 | 7 |
| GTAAA | 9610855 | 0.65845317 | 9.379459 | 6 |
| CATAC | 7246830 | 0.65767133 | 6.068537 | 5 |
| TAGAG | 7099250 | 0.65763 | 5.0876193 | 7 |
| TAGCC | 5351010 | 0.656603 | 5.6934433 | 7 |
| AGTGA | 6976145 | 0.64622635 | 9.375259 | 5 |
| TTAGC | 6295835 | 0.6433226 | 6.175095 | 6 |
| TAGCA | 6938510 | 0.6361818 | 5.928357 | 7 |
| GTGCA | 4968205 | 0.615915 | 6.9702578 | 6 |
| TGTGA | 5873120 | 0.60631526 | 13.376609 | 5 |
| TCACG | 4840000 | 0.5938988 | 8.003034 | 7 |
| TAAAC | 8709590 | 0.5906176 | 5.250422 | 7 |
| TGTAC | 5609810 | 0.573223 | 7.2777042 | 5 |
| CATAA | 8027580 | 0.544369 | 10.181514 | 5 |
| AGTAA | 7913985 | 0.54219824 | 8.695522 | 5 |
| GATAG | 5838290 | 0.54082257 | 10.963829 | 5 |
| ATAGC | 5851905 | 0.5365525 | 5.815782 | 6 |
| GTGAG | 4244930 | 0.5316747 | 5.5384383 | 6 |
| ATAGA | 7550045 | 0.5172641 | 7.3439612 | 6 |
| TGTAA | 6612870 | 0.5049093 | 12.757569 | 5 |
| TGTAT | 5885000 | 0.5007608 | 7.2609425 | 5 |
| GTAGC | 3963595 | 0.49137217 | 8.736372 | 6 |
| AGTAG | 5269170 | 0.48810288 | 8.632481 | 5 |
| CATAG | 4636765 | 0.42513815 | 9.901586 | 5 |