##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Undetermined_Undetermined_L006_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	100925331	
Filtered Sequences	0	
Sequence length	101	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.008442707014755	33.0	30.0	34.0	16.0	34.0
2	29.696490854213796	33.0	30.0	34.0	16.0	34.0
3	30.38044965143587	34.0	31.0	34.0	23.0	34.0
4	33.688109816553386	37.0	35.0	37.0	26.0	37.0
5	33.11635532807913	37.0	35.0	37.0	19.0	37.0
6	32.567679148855106	37.0	33.0	37.0	17.0	37.0
7	34.00234939036266	36.0	35.0	37.0	28.0	37.0
8	34.712960852216575	37.0	35.0	37.0	32.0	37.0
9	36.69954957095955	39.0	37.0	39.0	32.0	39.0
10-14	37.12642325047217	39.2	37.2	39.4	33.0	39.4
15-19	38.22991769033683	40.0	38.0	41.0	33.0	41.0
20-24	38.16425677514003	40.0	38.0	41.0	33.0	41.0
25-29	38.01308959987459	40.0	38.0	41.0	32.6	41.0
30-34	37.78458973198711	40.0	38.0	41.0	32.0	41.0
35-39	37.562833885578236	40.0	38.0	41.0	31.4	41.0
40-44	37.438978436444266	40.0	37.6	41.0	31.0	41.0
45-49	37.23796425299808	40.0	37.0	41.0	30.6	41.0
50-54	36.58864347147893	39.2	36.0	40.2	30.4	40.6
55-59	37.07776934216842	39.8	36.0	41.0	31.0	41.0
60-64	36.47460013978057	38.8	35.0	40.6	30.6	41.0
65-69	35.599235575457264	36.8	35.0	39.6	29.8	41.0
70-74	34.57595069764993	35.6	34.0	38.0	29.0	39.6
75-79	33.65983877526248	35.0	33.6	36.4	28.6	38.2
80-84	32.94473755453921	35.0	33.0	35.4	27.8	36.6
85-89	32.50240213133411	35.0	33.0	35.0	27.0	35.8
90-94	32.234235415091185	35.0	33.0	35.0	26.2	35.0
95-99	31.983195005820686	35.0	32.0	35.0	25.2	35.0
100-101	31.342029713036066	34.5	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	214268.0
3	8870.0
4	5757.0
5	5539.0
6	14184.0
7	84283.0
8	207096.0
9	176254.0
10	132400.0
11	120351.0
12	134311.0
13	167864.0
14	199120.0
15	219720.0
16	233605.0
17	253163.0
18	276853.0
19	305273.0
20	338640.0
21	377935.0
22	423725.0
23	479755.0
24	548804.0
25	642223.0
26	777205.0
27	1029035.0
28	1285087.0
29	1314985.0
30	1817146.0
31	1970568.0
32	2064328.0
33	2882464.0
34	4340657.0
35	7163511.0
36	1.3541868E7
37	2.8120186E7
38	2.6970605E7
39	2076503.0
40	1190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.738436931420349	19.68907048214775	32.40366623333161	33.16882635310029
2	32.779984299570266	30.679775399273794	17.04408598850841	19.496154312647533
3	15.688911541705819	52.30067815457756	14.588182736199643	17.42222756751697
4	18.650592698467065	18.47137165690399	16.342243884518734	46.53579176011021
5	32.97924719747929	14.260278036917438	34.60468660829761	18.155788157305658
6	33.29477373160149	34.97226787748816	30.785528465756475	0.9474299251538811
7	0.7558231204972381	1.2036002578182121	97.33572320228335	0.704853419401193
8	25.54264423690702	26.97771395769994	13.636518632831537	33.843123172561505
9	26.1744255192487	34.1561846395346	22.565494289188546	17.103895552028156
10-14	19.335627257408287	33.764657808222516	26.407000014466785	20.492714919902415
15-19	21.099298320094412	31.289305065622138	25.47602258415793	22.135374030125522
20-24	20.70201195254013	31.061120386855656	26.47665033068296	21.760217329921254
25-29	21.179429079018423	31.35410538321762	25.557460275611437	21.90900526215252
30-34	21.19582849370259	30.797790470857926	26.24301591796629	21.763365117473192
35-39	21.56218340907874	30.365464857269092	25.89172223210438	22.180629501547784
40-44	21.753018623471945	29.669493734325563	26.314042677227455	22.263444964975037
45-49	21.658708426660326	29.820823004760154	25.82844296053105	22.692025608048475
50-54	22.203165417689917	29.11197555485784	26.237788555065055	22.44707047238719
55-59	22.344288016371074	29.214116999935225	25.932972831677958	22.508622152015747
60-64	21.905410013097057	29.178717126778224	26.322530182075095	22.593342678049623
65-69	22.24176052386855	29.044303468810483	26.15185799438323	22.562078012937736
70-74	22.373128976529593	28.849323003313028	26.269081874724403	22.50846614543298
75-79	22.68028701145371	28.71102223820799	26.22580565509137	22.382885095246927
80-84	22.582901803394837	28.817255459039625	25.809941408777316	22.789901328788225
85-89	22.224380085747537	28.990389185827603	26.22342913953312	22.561801588891743
90-94	22.400346990077935	29.122957030726447	26.29441169047213	22.18228428872349
95-99	22.727155438774922	29.570377836378825	25.70141237342159	22.001054351424667
100-101	22.934321975216594	29.064984124906466	25.92340892061581	22.077284979261126
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	47.907263284520646
2	52.27613861221779
3	33.11113910922279
4	65.18638445857728
5	51.135035354784954
6	34.24220365675538
7	1.460676539898431
8	59.38576740946853
9	43.27832107127686
10-14	39.8283421773107
15-19	43.23467235021993
20-24	42.46222928246139
25-29	43.08843434117094
30-34	42.959193611175785
35-39	43.742812910626526
40-44	44.01646358844698
45-49	44.3507340347088
50-54	44.650235890077106
55-59	44.85291016838681
60-64	44.49875269114668
65-69	44.80383853680628
70-74	44.88159512196257
75-79	45.06317210670064
80-84	45.372803132183066
85-89	44.78618167463927
90-94	44.58263127880142
95-99	44.72820979019959
100-101	45.01160695447772
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	197952.0
1	103640.0
2	8771.5
3	8503.0
4	7734.0
5	6290.0
6	5797.5
7	5942.0
8	6372.0
9	6184.0
10	6045.0
11	6491.5
12	6770.0
13	7321.5
14	8055.0
15	8796.0
16	9799.5
17	11208.5
18	12926.5
19	15703.5
20	21606.5
21	31604.0
22	47387.0
23	61600.0
24	80028.5
25	108462.5
26	144274.5
27	200068.0
28	296901.0
29	410573.0
30	515954.5
31	675087.5
32	904725.5
33	1204350.5
34	1547692.0
35	1895772.0
36	2295514.0
37	2843491.5
38	3519987.5
39	4205003.0
40	4805809.0
41	5406139.5
42	6092879.0
43	6636694.0
44	6870079.5
45	6807150.0
46	6673915.5
47	6345399.5
48	5803715.5
49	4896507.0
50	3631542.5
51	2757241.0
52	2429503.5
53	2201840.5
54	1856491.5
55	1524200.0
56	1140103.5
57	865596.5
58	692576.0
59	520223.5
60	395179.0
61	276829.5
62	198490.0
63	144867.0
64	114640.0
65	94276.0
66	78848.0
67	65829.5
68	53827.0
69	43114.5
70	34000.5
71	26216.0
72	20017.0
73	15266.5
74	11166.0
75	8046.0
76	5901.0
77	4308.0
78	3159.5
79	2354.5
80	1788.5
81	1363.0
82	1101.5
83	918.0
84	781.5
85	675.5
86	563.5
87	474.0
88	440.5
89	392.5
90	316.0
91	247.0
92	209.5
93	176.5
94	149.5
95	120.5
96	73.5
97	57.5
98	53.0
99	29.5
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2625327035092905
2	0.2619679295379274
3	0.26529415097979714
4	0.2711360936730542
5	0.2639535559214564
6	0.2434423524456908
7	0.23976735830571616
8	0.2462711764601495
9	0.24958748958673221
10-14	0.2510333109534216
15-19	0.2489176874733262
20-24	0.25231148362545375
25-29	0.243367049249509
30-34	0.2652317285934886
35-39	0.24444765011471697
40-44	0.2616472964552378
45-49	0.2625794707574454
50-54	0.2724969016945805
55-59	0.2754038032335014
60-64	0.2639382971159144
65-69	0.2603570356385554
70-74	0.26099057331801073
75-79	0.27548148442535203
80-84	0.27396610668534743
85-89	0.26773912686003476
90-94	0.2780360462726647
95-99	0.27738407912677543
100-101	0.2828343461167346
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.00925331E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	89.90366164088506
#Duplication Level	Relative count
1	100.0
2	64.87723517318956
3	48.15561847872464
4	35.277917846476086
5	27.13238431402971
6	22.77258446142508
7	18.490361118653272
8	15.837244482370739
9	14.146076568015205
10++	429.0756758853419
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	199476	0.19764711002037733	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGACC	26419450	3.2418346	178.11005	1
CAGTG	24761495	3.0697157	187.71373	2
GCTTT	25871135	2.9461267	150.9761	3
CGATG	23708535	2.9391787	177.42957	1
GACCA	26244770	2.8896773	161.70708	2
AAGAA	44496080	2.735421	5.370185	9
TGGTG	19395315	2.7072773	6.801635	7
CTCAG	20846550	2.5580041	187.64311	1
TGTTG	22187155	2.5526528	37.479603	4
TCAGA	27352700	2.5079288	139.65166	2
CAGAT	26367470	2.4175944	141.50386	3
CTTTG	21189450	2.4129903	40.23279	4
ACAGT	25883295	2.3732011	142.19025	1
GATGT	22798635	2.3536315	134.31598	2
ACCAT	25931055	2.35332	134.79927	3
TGCTG	16745660	2.3135774	8.318487	7
AGTGT	22331095	2.3053646	162.2338	3
CAAGA	27706160	2.279454	5.533038	8
AGCTT	22247070	2.2732556	132.11409	2
TGAAG	24097105	2.2322047	8.192987	7
GATCA	23628910	2.1664999	112.225815	1
TTGGT	18593275	2.1391735	5.742917	6
TTTGG	18316795	2.1073642	10.768336	5
ATGTT	24267480	2.064945	110.610245	3
ATCAG	22092255	2.0256064	111.94866	2
GTGTC	14389730	1.9880823	74.67898	4
CCATC	16354440	1.9863169	61.9723	4
TCAAG	21634385	1.9836249	13.787529	7
TAGCT	19391410	1.9814578	132.27246	1
TTGAA	25386610	1.9383316	7.5603843	6
TTCCA	19106780	1.9324523	5.004672	6
TCAGT	18758365	1.9167719	124.8548	3
CAACA	23250390	1.8933485	5.0109816	8
TGCCA	15252585	1.8715892	10.849487	7
TCCAA	20615675	1.8709337	6.285258	7
GTTGC	13448120	1.8579897	11.743615	5
GTGGT	13090340	1.8272029	7.527871	6
CTTTC	16017795	1.8054458	53.26202	4
TGGTT	15631225	1.7983869	5.037378	7
TGGCC	10727350	1.7797778	5.269875	7
TTGCC	12967815	1.7733495	7.060593	6
TGTTC	15286815	1.7408162	50.853756	4
AGATC	18881930	1.7312564	49.50476	4
TTCTT	18433445	1.7302012	7.384216	6
ATGGT	16721935	1.7262995	14.233323	3
GTTGA	16357570	1.6886841	10.539224	5
TTGGC	12156345	1.6795183	5.4491844	6
GTCAA	18269485	1.6751021	19.197924	6
GTTGG	11995635	1.6743997	10.743874	5
TGTCG	12077440	1.6686169	17.537298	5
TTGCT	14601310	1.6627531	6.715682	6
TGGTC	11889715	1.6426808	6.624097	4
CCATG	13353300	1.6385348	47.427864	4
TTTGA	19184100	1.6323948	9.438898	5
TCAAA	23860320	1.6180241	15.839358	7
GTTCT	14035335	1.5983015	12.781206	5
TTCTG	13945580	1.5880805	5.758421	6
TTGTT	16742735	1.5877085	6.112738	6
CAAAA	26027245	1.5837128	5.670093	8
TGTCT	13826150	1.5744802	15.996114	5
GTGTT	13638235	1.5690916	23.773767	4
TTCAA	20731775	1.5667715	14.681015	6
TCTTC	13822215	1.557971	9.009939	7
CCATT	15320315	1.5494907	20.436655	4
TTTTG	16300335	1.5457556	7.752784	5
ATCAA	22595210	1.5322341	15.44457	6
TGCTT	13381385	1.5238317	5.3621793	7
TCAAC	16781270	1.52295	11.588974	7
AGATG	16333865	1.5130669	35.752445	4
GATGA	16324135	1.5121655	11.205836	5
TTTCT	15978920	1.4998143	12.414132	5
TGGCT	10816010	1.4943379	7.5823703	7
TGTCC	10902315	1.4908923	10.415979	5
TGGAA	15793590	1.4630191	7.778444	7
CATTG	14241900	1.4552693	8.781849	5
ATGTC	14234050	1.4544673	5.160859	6
TTGGA	13937880	1.4388858	5.829828	6
GTGCT	10396260	1.4363453	9.153478	6
TCTTT	15298030	1.4359045	8.236224	7
GTCGC	8622510	1.4305631	10.937722	6
GATGG	11313850	1.4170521	12.079972	5
TCTGC	10329760	1.4125953	5.2824516	7
CATCA	15532960	1.409662	19.465328	5
TGTCA	13636860	1.393445	23.319714	5
TCCCA	11409705	1.3857577	6.095592	7
CTTTT	14668990	1.3768616	16.957943	4
TGTTT	14482130	1.373336	16.421944	4
TGAAA	20005365	1.3705957	9.350331	7
TTGTC	12000725	1.3666062	5.029928	6
GTTGT	11833825	1.3614925	8.398963	5
TTTCA	15978025	1.3457139	17.270292	5
GTTCC	9788435	1.3385692	8.570578	5
TTTCC	11861950	1.3370197	8.004644	5
GTTTG	11601295	1.3347397	8.321741	5
GTCAC	10862195	1.3328604	10.1459	6
TCTTG	11679260	1.3299987	5.3194947	7
CATGG	10663270	1.3219398	11.465214	5
TTTGC	11573005	1.3178988	10.635375	5
CATCT	12837205	1.2983499	13.725153	5
GATTG	12532140	1.2937634	9.285824	5
TGTGG	9177230	1.2809952	14.071291	5
TCGGT	9253990	1.2785295	6.6990047	7
TCTGG	9208525	1.272248	5.36116	7
TCGCT	9237285	1.2631992	9.405355	7
TTGCA	12280560	1.2548553	5.15645	6
TTCTC	11128510	1.2543502	7.3651013	6
AGTTG	12097120	1.2488538	7.306168	5
TGCAA	13480670	1.2360228	6.577816	7
GATCG	9962125	1.235018	14.694429	5
AGATT	16119025	1.230728	15.791097	4
ATGGC	9913695	1.229014	5.503867	6
TGAAC	13399730	1.2286015	5.2586694	7
TCTGA	12016150	1.2278372	5.092735	7
TGCTC	8967275	1.2262754	5.129742	7
GCCAT	9976180	1.2241408	5.6939373	8
TCTCT	10805010	1.2178868	5.905879	7
GTCCA	9915565	1.2167029	6.175087	6
GTGTG	8697760	1.2140689	56.50998	4
CCCAG	8223390	1.2117404	6.029328	1
TGTTA	14160755	1.2049533	30.903782	4
CAGTA	13124560	1.2033715	30.22025	4
ATGAA	17277225	1.183687	7.9534287	6
CAGTC	9581360	1.1756938	50.58326	4
TCTCC	8636600	1.1690044	5.9605513	7
CTTTA	13757495	1.1586946	30.218401	4
GTCGT	8379810	1.157753	7.286793	6
ATCTC	11426600	1.1556818	7.4157166	6
TTTGT	12155295	1.1526828	8.534418	5
GTGCC	6929640	1.1496986	9.699617	6
GATGC	9257000	1.1476027	11.71448	5
CGCTT	8386100	1.1467998	5.9098105	2
GTCGA	9241210	1.145645	9.98275	6
GCCTT	8258265	1.1293184	5.249904	3
ATCCA	12383650	1.1238528	5.170463	6
GTTCA	10997255	1.1237242	18.853516	5
TTATT	15838855	1.1108664	5.066848	6
TCGCC	6669485	1.0952455	7.5508714	7
TCGTT	9503660	1.082248	5.68631	7
GTGGA	8622415	1.0799518	7.9682226	6
GTGGC	6438705	1.0792599	7.4489675	6
ATGCT	10430575	1.0658196	6.6617994	6
GTGAA	11380810	1.0542469	10.44011	6
TCACT	10408440	1.0527055	5.779047	7
GTCTC	7677455	1.0498925	9.854527	6
ATCGG	8467595	1.0497391	5.3536453	6
TCCGT	7646090	1.0456033	5.1143913	3
CAGTT	10196030	1.0418533	16.262608	4
AAAGT	15190430	1.0407177	8.914279	1
AGTTT	12227325	1.0404358	8.868363	3
ATGGA	11201130	1.0376024	5.9677277	6
CATCG	8380870	1.028386	13.3833	5
GTCTT	9026005	1.0278542	9.104418	6
ATCTG	10021870	1.0240573	5.972892	6
GTCTG	7403365	1.0228475	7.3721423	6
GTCAT	9939055	1.015595	6.173609	6
CATCC	8320040	1.0105046	8.570502	5
TCTCA	9881940	0.9994556	6.945116	7
TCACA	10945445	0.9933315	8.239804	7
GTTAC	9704910	0.99166954	6.220001	5
GTGTA	9592340	0.9902713	43.777637	4
TATTT	13968010	0.97965366	5.3411274	7
ATCTT	11577630	0.9751003	7.7984543	6
TTCAG	9515230	0.9722876	7.144936	6
TAAGA	14174945	0.97114533	6.2026362	7
AGTCA	10569825	0.9691317	15.28481	5
TTTAT	13705435	0.9612378	5.6559577	5
TTTAC	11402155	0.96032125	5.438348	5
ATGCC	7782565	0.95497024	7.4098186	6
CATGA	10410965	0.95456606	10.594365	5
TTCAC	9411670	0.9518926	7.8739147	6
TCAAT	12583495	0.950978	9.232019	7
TCAGC	7683970	0.942872	8.559115	7
AGATA	13681175	0.93731654	28.754644	4
TCGAA	10211920	0.9363159	8.781966	7
TAGAA	13380285	0.9167021	9.943952	7
GTCCC	5538300	0.90948534	5.8700037	6
ATATT	14329320	0.90178514	5.294715	6
TTTCG	7916715	0.9015315	12.295211	5
GATCT	8809885	0.90021384	14.09078	5
CCATA	9901915	0.89862806	35.900616	4
GTAGA	9675390	0.8962675	9.484986	6
GTCAG	7214190	0.8943528	9.801991	6
TGGGA	7100605	0.88934606	5.240731	7
GTTAT	10444985	0.88877463	6.112788	5
TCTCG	6480505	0.8862094	6.3662086	7
GTAAG	9542770	0.88398236	5.8520656	6
ATCGA	9638035	0.8836972	7.299382	6
TATTC	10457885	0.8807923	5.9366083	7
TTTAG	10324820	0.8785496	9.063952	5
TTCGT	7635565	0.86951506	5.4702373	6
CATGC	7077125	0.86840826	12.102259	5
GTCGG	5109545	0.8564653	7.3980885	6
GTTCG	6185510	0.8545889	14.031433	5
TTCGG	6111770	0.84440106	5.310041	6
TGTGC	6088515	0.8411881	14.903409	5
ATGCA	9170675	0.8408457	5.1814847	6
TTTAA	13114605	0.8253397	8.940947	5
GTGGG	4871755	0.82502496	5.9864955	6
GTAAC	8840355	0.81055915	5.7159276	6
TAAAG	11603230	0.7949535	5.182794	7
TTCGC	5793880	0.79231346	7.879975	6
TCGAG	6388375	0.7919753	5.015033	7
ATCAC	8711960	0.7906362	8.398835	6
TTCGA	7730040	0.7898729	7.2357073	6
CATGT	7725240	0.7893824	9.653819	5
GTTAA	10296770	0.7861843	10.633393	5
ATCGT	7626160	0.7792582	5.5894446	6
GATAT	10172855	0.776723	6.5756273	5
AGTCT	7579400	0.77448016	10.332277	5
TTAGA	10134160	0.77376866	7.2454314	6
CGACG	5157425	0.7677959	5.4226832	1
TCGAT	7503795	0.7667546	5.197082	7
GATCC	6196530	0.7603536	9.243943	5
TAAAA	14996060	0.7598602	8.556302	7
GATAA	11052905	0.7572501	11.153832	5
GTCTA	7391060	0.75523514	5.9670453	6
GTATT	8860905	0.7539836	6.3999243	6
AGTCC	6101675	0.7487143	6.762257	5
TGTAG	7241115	0.74754107	12.808862	5
ATCGC	6040300	0.7411832	7.6982126	6
AGTGG	5916415	0.74102706	10.052288	5
AAGTG	7907080	0.73246235	5.1545105	2
TTAAA	12952515	0.7314273	6.897596	6
GTGAT	7030235	0.7257708	5.9043646	6
TGTGT	6175515	0.71049875	10.345795	5
AGTGC	5526840	0.68516976	10.0865555	5
CATAT	9043255	0.68342984	6.46008	5
AGTCG	5504225	0.68236613	11.849093	5
GTTAG	6543655	0.67553836	10.667192	5
TCGCA	5427690	0.6660121	6.4453974	7
ATAAA	13123345	0.66496855	6.970068	6
GATAC	7240975	0.6639143	6.4273286	5
TCGAC	5392795	0.66173023	5.4893603	7
GTAAA	9610855	0.65845317	9.379459	6
CATAC	7246830	0.65767133	6.068537	5
TAGAG	7099250	0.65763	5.0876193	7
TAGCC	5351010	0.656603	5.6934433	7
AGTGA	6976145	0.64622635	9.375259	5
TTAGC	6295835	0.6433226	6.175095	6
TAGCA	6938510	0.6361818	5.928357	7
GTGCA	4968205	0.615915	6.9702578	6
TGTGA	5873120	0.60631526	13.376609	5
TCACG	4840000	0.5938988	8.003034	7
TAAAC	8709590	0.5906176	5.250422	7
TGTAC	5609810	0.573223	7.2777042	5
CATAA	8027580	0.544369	10.181514	5
AGTAA	7913985	0.54219824	8.695522	5
GATAG	5838290	0.54082257	10.963829	5
ATAGC	5851905	0.5365525	5.815782	6
GTGAG	4244930	0.5316747	5.5384383	6
ATAGA	7550045	0.5172641	7.3439612	6
TGTAA	6612870	0.5049093	12.757569	5
TGTAT	5885000	0.5007608	7.2609425	5
GTAGC	3963595	0.49137217	8.736372	6
AGTAG	5269170	0.48810288	8.632481	5
CATAG	4636765	0.42513815	9.901586	5
>>END_MODULE