FastQCFastQC Report
Thu 31 Jul 2014
Undetermined_Undetermined_L006_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename Undetermined_Undetermined_L006_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 100925331
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 200291 0.1984546377162736 No Hit
CTCAGATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 199541 0.1977115140697433 No Hit
ACAGTGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 189790 0.1880499158333204 No Hit
CGATGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 174286 0.17268806381224552 No Hit
TAGCTTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 109923 0.10891517413007049 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CAGTG 34032150 4.060983 181.32185 2
GACCA 24769075 3.410898 206.83307 2
ACCAT 29061460 3.0643396 159.89021 3
TGACC 25256450 3.0499177 178.66792 1
TCTTC 37451550 3.0367084 8.038016 7
TTCTT 48495485 2.975234 6.020792 50-54
ACAGT 27985660 2.9159565 164.69962 1
GCAGC 17918910 2.8259652 8.055082 8
TCAGA 26512355 2.7624462 157.43806 2
AGCTT 29330280 2.6799002 115.39357 2
CGATG 22267710 2.657158 176.11542 1
CACCA 18844850 2.6261866 6.2008505 40-44
CTCAG 21441705 2.5892565 188.75282 1
CAGAT 24302735 2.5322154 158.94293 3
AGTGT 27846355 2.514182 142.30667 3
ATCAG 22878430 2.3838103 124.02254 2
TGTTG 29484995 2.3344595 55.103832 4
GATCA 22396650 2.3336115 123.73323 1
GATGT 25138315 2.2696795 122.81149 2
CAACA 18736305 2.252924 5.090407 35-39
TCTTG 27647795 2.2152348 6.312429 7
TCAGT 23084645 2.109238 109.64282 3
CCATG 17459120 2.1083276 88.19407 4
TGGAG 17623845 2.07811 5.069792 7
GCGGC 11446450 2.0673897 36.92825 8
GCTTT 25554565 2.0475183 105.47821 3
GGCAG 12981855 2.0231032 10.456888 8
TAGCT 21929745 2.0037148 115.24184 1
CTTTG 24591085 1.9703209 54.11873 4
TCAGC 16168035 1.952419 6.2785873 7
TGGCA 16243390 1.9382888 14.889785 7
AGATG 18711775 1.9265776 73.676476 4
TTTCT 31265855 1.9181834 10.884274 5
TTGGC 17982880 1.8817326 26.883682 6
ATGTT 26205970 1.8117121 94.50051 3
CCTTC 16719230 1.7916903 5.516329 9
CCATC 14567210 1.7801892 60.32857 4
CATCG 14484230 1.7490859 15.19398 5
CGGCA 11083210 1.7479169 19.006638 9
GTTGG 16746510 1.7316056 22.31907 5
TTTGG 21614230 1.7112956 18.055904 5
GATGG 14366010 1.6939632 26.026587 5
TGTTC 21056100 1.6870861 33.400276 4
AGATC 15948240 1.6617215 52.163578 4
GGAGG 10705935 1.6486628 5.3798594 75-79
TGGTG 15860350 1.6399759 6.099986 4
AGTGG 13855585 1.6337765 19.721897 5
TGCTG 15594190 1.6317798 13.050722 7
AGCGG 10464975 1.6308703 8.480157 8
ACACC 11567970 1.6120929 5.5549874 40-44
GCCTT 14943255 1.5824059 7.297834 8
GTGTC 14993415 1.5689143 56.51696 4
CTTTC 19337115 1.5679239 34.96881 4
TCTGG 14962575 1.5656874 14.193415 7
TTCTG 19510905 1.5632795 7.865441 6
TCCTT 19224265 1.5587736 5.202809 7
GGTCA 12865030 1.5351564 5.734031 60-64
ATGGC 12761705 1.5228267 30.086617 6
CTTTT 24781075 1.5203375 6.988878 4
TCTCT 18696355 1.5159687 11.506725 7
GTTCT 18836555 1.5092486 11.611148 5
CATGG 12627800 1.5068481 25.016867 5
GTGGA 12727600 1.5007708 5.2029295 6
GTGTG 14428775 1.4919496 76.75318 4
CAGTC 12331730 1.4891546 47.84217 4
GTCTT 18427885 1.4765046 6.0067687 6
TGGCG 10641445 1.4542488 29.341682 7
TGGCC 10391380 1.4370946 40.13117 7
GGCGG 8023860 1.432059 15.514329 8
GTGGC 10451580 1.428302 31.817095 6
TTCTC 17510345 1.4198025 12.150211 6
GCTGG 10353245 1.4148636 5.5569196 8
GTTGT 17506215 1.3860457 12.468211 5
AATAA 17586880 1.3805995 5.051133 20-24
TGTCC 13035615 1.3803977 23.33 7
CGGCG 7580515 1.3691475 13.195141 8
GTAGC 11438635 1.3649478 8.144326 6
AAAGT 14988050 1.3474737 12.55378 1
AGCCT 11091970 1.3394438 6.19486 2
GTTGA 14834610 1.3393823 5.139295 5
TTTCC 16512415 1.3388867 12.651309 5
TGGTC 12764640 1.3356948 5.431655 7
GGCCA 8410565 1.3264179 8.333218 8
GATGA 12771930 1.3150069 6.0750093 5
ATCTT 18567455 1.2990184 5.8506994 6
TTGCC 12235000 1.295617 26.87658 6
GTTGC 12309240 1.2880416 30.926239 5
TTGCT 15928740 1.2762645 7.97244 6
GCGGT 9262975 1.2658685 6.7676744 8
TGTGG 12187635 1.2602135 21.673376 5
CCGGC 6852470 1.2524853 17.263878 9
TTGGG 12109665 1.2521513 10.964201 6
CATCT 13472715 1.2457516 16.539942 5
CAGTT 13556665 1.2386689 9.229213 4
TTTGC 15454255 1.2382472 23.73788 5
TTGCA 13525420 1.235814 5.6071115 6
CTCTG 11629945 1.2315452 5.7026405 8
GATGC 10301925 1.2293065 34.156174 5
TGCCC 8765030 1.2267033 46.40446 7
CAGTA 11651840 1.2140596 13.901529 4
AGTAG 11726395 1.2073579 5.1286464 5
ATGGT 13085720 1.181479 10.677562 3
CCCTT 11015045 1.1804101 5.2435565 8
TTGTC 14588300 1.1688639 12.934995 6
TGTCT 14532020 1.1643547 14.906952 5
ATCGG 9726440 1.160635 14.512768 6
AAGTT 14658260 1.1556185 5.6406646 2
TGGCT 11032700 1.1544644 12.100987 7
TGCGG 8396740 1.1474898 42.157898 7
CCCAG 7122040 1.136668 6.1346426 1
GAGAT 10935455 1.1259222 5.2246923 95-97
AGTTT 16122710 1.1146204 9.51619 3
GCCCA 6981750 1.1142778 11.94113 8
TACCG 9225090 1.114003 7.496097 7
TGCAG 9319210 1.1120414 11.877344 7
GCCCC 6000895 1.109981 16.33043 8
CCATA 10495875 1.1067209 17.855469 4
CCATT 11965440 1.1063818 12.018593 4
TGTGC 10559695 1.1049689 35.692413 5
TGCTT 13761030 1.1025804 5.605356 7
TCTGC 10376290 1.0987903 9.132976 7
GCTGC 7933365 1.0971588 5.1049027 8
AAGTG 10637285 1.0952224 5.2568226 2
GTTTG 13815025 1.0937977 6.3053155 4
GCCTC 7799865 1.0916243 7.60775 8
ATCTG 11943795 1.0913013 9.641591 6
TCCAG 8964200 1.0824986 16.011066 9
TTTGT 17700555 1.0730815 10.697671 5
GCGGA 6849500 1.0674317 9.542233 9
GCCAG 6766710 1.067168 5.066405 8
GATCT 11678835 1.067092 16.920555 5
AGGGG 6898295 1.0623045 5.302782 75-79
ATGGG 8973370 1.0580919 11.81528 6
CCGGA 6688445 1.0548248 5.3669987 9
GGGTC 7694140 1.0514731 5.0734878 60-64
TGCCA 8700820 1.0506934 9.46991 7
TGTAG 11601305 1.0474547 5.557777 5
CTGGC 7552635 1.0445052 6.7745624 8
TAGCC 8613255 1.040119 6.8231497 1
TGTCA 11376225 1.0394427 6.1365113 7
GGGCA 6653415 1.0368737 7.438612 8
TGTTT 16870410 1.0227548 6.959466 4
GGCGA 6553500 1.0213028 5.9045267 8
CCCGG 5570735 1.0182115 12.205449 8
TTGTG 12811800 1.0143677 8.044884 6
GTGCC 7304380 1.0101724 38.287636 6
AGTCC 8347980 1.008085 16.59475 5
TCTCC 9383955 1.005617 25.466427 7
ATGTC 10954790 1.0009362 14.601663 6
CTCCA 8153430 0.9963917 15.960738 8
AGATA 11069275 0.9951633 16.20693 4
GTGTT 12525960 0.99173653 10.393514 4
TGACG 8272150 0.9870979 5.7888856 7
CGCCA 6182240 0.9866771 5.074634 9
GGCCC 5377515 0.982895 21.769688 8
TTAGC 10735480 0.980898 6.329521 6
CCGCC 5291480 0.978761 8.354752 9
GATCG 8188395 0.9771035 14.665046 5
TCGGC 7053905 0.9755325 27.592012 7
CATTG 10673270 0.97521377 5.401461 5
GGCCT 7049810 0.9749661 11.055186 8
CATGT 10642440 0.97239685 15.028204 5
GTCCC 6946005 0.97212297 23.942278 6
GTTCC 9171610 0.9712215 15.55473 5
TCCGG 7000325 0.9681225 21.887104 7
CGGCC 5296630 0.968111 10.069055 9
CATCC 7918130 0.96763676 19.430275 5
GTCTG 9135840 0.9559765 10.382419 6
TGGGC 6995325 0.95597386 25.797789 7
TGCCG 6904175 0.9548253 29.127897 7
CCGGG 5283715 0.9543131 6.701852 8
GGCGT 6971885 0.9527706 7.427695 8
GCCCT 6784890 0.94957423 13.124038 8
CCGTC 6735420 0.9426507 5.67329 9
ATAGC 8992725 0.93699396 7.567047 6
GTGTA 10359810 0.93536305 15.263325 4
CCGGT 6734435 0.9313507 6.135235 9
GTGCT 8888495 0.93009424 10.776697 6
GATAG 9017300 0.9284276 6.763936 5
GATTG 10125005 0.91416293 5.294766 5
CGCCC 4939975 0.91374344 5.321252 70-74
CGGCT 6594835 0.9120445 11.047549 9
TCCCC 6424725 0.90994394 16.468054 7
GCGCC 4973670 0.90908074 13.326279 8
AGATT 11514895 0.90780395 10.42441 4
TGGGG 6701075 0.90491676 5.036249 7
GTCTC 8538125 0.90413904 16.492731 6
CCTCG 6453105 0.9031395 5.70434 85-89
ATGCC 7429015 0.89711267 32.16663 6
GCCGG 4955745 0.8950771 17.725447 8
GTCCT 8437575 0.89349127 6.8495383 8
TGCTC 8427905 0.8924674 9.497716 7
GCCTG 6436600 0.89016116 7.0371523 8
GATCC 7354680 0.8881361 19.24304 5
TTGCG 8406030 0.87960887 20.824797 6
TAGCG 7371035 0.87956965 12.349853 7
TTTCG 10952685 0.8775661 9.365535 5
GTGCG 6374410 0.8711203 27.480658 6
TGTGT 10988640 0.87002003 13.498871 5
TGTGA 9603325 0.8670618 5.057558 5
GTCGG 6341850 0.86667067 17.093288 6
TTCCC 8069425 0.86474735 15.689725 6
AGTCT 9461745 0.8645171 11.979501 5
CATGC 7154705 0.8639875 38.805275 5
ATCCT 9320225 0.86179256 5.863898 6
GTACC 7102355 0.85766596 5.00873 6
CTTTA 12222825 0.8551348 10.929042 4
CCCGC 4576805 0.8465681 10.920281 8
ATGCA 8121090 0.84617424 6.461822 6
AGTGC 7079880 0.84482676 30.16067 5
TGCCT 7947280 0.8415718 19.073753 7
TTCGG 8041570 0.8414717 11.883404 6
CATGA 8056760 0.8394714 5.629296 5
ATCTC 9027430 0.8347193 16.144026 6
GTGGG 6152920 0.83089364 12.758048 6
CGCTT 7570970 0.80172276 5.9971213 2
GCCGC 4373405 0.7993651 10.508444 8
TGTTA 11557850 0.79903543 10.916681 4
CGAGA 5857465 0.79706657 5.762216 95-97
GCGTG 5796950 0.79220515 5.4486265 8
TCCCG 5635180 0.78866744 24.725712 7
TCCTG 7428850 0.78667307 6.8581047 7
CATAG 7459170 0.7772057 6.3076034 5
TGTCG 7424265 0.77687687 14.134968 5
CCCCG 4172030 0.7716972 7.9527416 8
TGCGC 5551130 0.7677035 21.422077 7
ATGCT 8389025 0.7665029 9.733999 6
CCCCT 5406560 0.7657396 5.7464967 9
TCCCA 6198520 0.7574915 6.214435 7
ATCCG 6146155 0.7421971 11.031886 6
GGCGC 4082895 0.73742825 6.608506 8
ATGTG 8160180 0.73676354 9.034449 6
GTTCG 7026890 0.7352955 11.147231 5
TCCCT 6854225 0.7345224 13.753215 7
TCGCC 5195845 0.7271806 11.985095 7
GGGCC 4001130 0.72266036 16.51886 8
ATCCC 5885275 0.7192113 18.63071 6
CCCTG 5134360 0.71857554 6.3845444 8
GCGCT 5195240 0.71848506 6.2220507 8
GCCCG 3814760 0.69725674 13.806306 8
CGGGC 3845800 0.6946055 7.8087087 8
TTCGC 6526310 0.6910991 7.704537 6
CCGCA 4294265 0.6853589 6.6683617 9
CCGTG 4949020 0.6844336 5.302843 4
AGTCG 5650440 0.6742548 12.534733 5
GCCGT 4852305 0.6710582 6.217361 8
CCCAC 4132480 0.6674426 6.0923843 9
TGCGT 6358260 0.6653299 9.654981 7
TTCCG 6216355 0.6582767 9.56989 6
TCCGC 4653000 0.65120715 10.391552 7
ATGCG 5360380 0.63964254 23.27335 6
TGCGA 5342850 0.63755083 5.054835 7
TCTCG 5971950 0.6323956 7.4418125 7
GTTAG 6864430 0.6197734 5.300889 5
GTGCA 5181510 0.6182985 7.4551277 6
CTCCC 4337620 0.61434394 6.745351 8
GGCCG 3254865 0.5878744 7.060102 8
CGCGG 3153035 0.5694824 5.1912537 8
GTCCG 4099470 0.5669436 14.505646 6
TCGCT 5204080 0.55108255 7.214176 7
CCCGT 3754090 0.5254009 5.8682547 9
TCGGG 3734245 0.51031804 11.728187 7
ATCGC 4158715 0.5021979 10.382507 6
CCCCC 2678375 0.5013544 5.450506 9
GTCGC 3532085 0.48847604 11.839348 6
TCGCG 3507140 0.4850262 9.46484 7
Produced by FastQC (version 0.10.1)