FastQCFastQC Report
Thu 31 Jul 2014
B80b_GATCAG_L006_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename B80b_GATCAG_L006_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 400514
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CAGTG 107580 3.3610375 214.94926 2
TGACC 103640 3.203343 173.98749 1
GCTTT 105165 3.026468 157.30902 3
GAAGA 137870 2.864985 5.0585837 9
GACCA 102730 2.8384464 157.45235 2
AAGAA 177590 2.7164946 5.526107 9
TGGTG 76345 2.6969988 6.9889393 7
CTCAG 86925 2.6867096 200.32697 1
TCAGA 113895 2.6192937 148.49788 2
ACAGT 112055 2.5769787 162.04843 1
AGTGT 98260 2.55514 185.84587 3
CAGAT 108895 2.5043068 150.50569 3
CGATG 79975 2.4985962 133.19566 1
TGTTG 84685 2.463412 27.791899 4
CTTTG 84260 2.4248576 42.749718 4
AGCTT 90420 2.3261445 136.75638 2
ACCAT 101835 2.3169193 131.16393 3
TGCTG 66175 2.3127487 9.083437 7
CAAGA 110530 2.2723076 5.5129585 8
TGAAG 95250 2.2141664 7.7888665 7
GATCA 96060 2.2091343 113.538734 1
TTGGT 73640 2.1421227 5.0780253 6
TTTGG 73305 2.1323779 11.328091 5
GTGTC 60755 2.123325 86.052414 4
ATCAG 89395 2.0558565 113.43976 2
TAGCT 78980 2.0318391 136.9044 1
GATGT 77080 2.004378 101.21568 2
TCAAG 86485 1.9889338 14.195767 7
CCATC 64080 1.9594439 61.42453 4
TCAGT 75115 1.9324081 126.88646 3
TTGAA 100370 1.9212309 6.7106547 7
TCCAA 83180 1.8924862 6.575231 7
TGCCA 61090 1.8881923 10.401274 7
CAACA 92145 1.874101 5.148306 8
CTTTC 65065 1.85245 55.63112 4
GTGGT 52100 1.8405088 7.55441 6
GTTGC 52320 1.8285306 8.627061 5
TGGTT 62045 1.8048345 5.2330565 7
ATGTT 83235 1.7822733 83.27204 3
AGATC 77430 1.780692 52.654434 4
TGGCC 42400 1.7803451 5.802754 7
TTGCC 50480 1.7453722 6.3878613 6
TTCTT 73035 1.7307179 6.871606 6
TGTCG 48585 1.6979961 19.93207 5
GTCAA 73340 1.6866326 20.496838 6
ATGGT 64750 1.6837504 10.241387 3
TTGCT 58160 1.6737448 5.9308314 6
TTGGC 47740 1.6684641 5.016351 6
GTTGA 63830 1.6598269 7.894468 5
TTTGA 77225 1.6535839 9.958583 5
TGTTC 57035 1.6413692 39.414043 4
GTTGG 46390 1.6387948 7.7608447 5
TCAAA 96610 1.6354579 15.924652 7
GTGTT 55795 1.6230274 27.227596 4
CCATG 52380 1.6189802 45.749043 4
TGTCT 55865 1.6076988 18.617926 5
TCTTC 55685 1.5853943 8.959726 7
CAAAA 104390 1.5797325 5.738575 8
GTTCT 54890 1.5796399 9.99283 5
TTCTG 54815 1.5774814 5.205177 6
TTTTG 65720 1.574195 8.096631 5
TTCAA 82555 1.5633397 13.76491 6
TTGTT 65235 1.5625778 5.7146273 6
CCATT 61300 1.5601519 18.909786 4
TGCTT 53480 1.5390625 5.232956 7
ATCAA 90550 1.5328714 15.92505 6
AGATG 65725 1.5278329 37.981113 4
TCAAC 67130 1.5273215 11.804525 7
TGTCC 44145 1.5263363 11.888458 5
TGGCT 43600 1.5237755 7.710753 7
TTTCT 63485 1.5044105 13.078204 5
GATGA 64635 1.5024949 11.341064 5
TGGAA 63295 1.4713457 7.4619827 7
ATGTC 56970 1.4656099 5.2893195 6
TCTTT 61410 1.4552388 8.49162 7
TGTCA 55915 1.4384692 26.686623 5
TTGGA 55190 1.4351535 5.2960124 6
GTCGC 34150 1.4339336 11.66688 6
CATTG 55705 1.4330667 8.5337305 5
TCTGC 41230 1.4255487 5.331468 7
GTGCT 40665 1.4212002 9.744602 6
TCCCA 46360 1.4176003 6.331246 7
GATGG 44425 1.4029266 13.247509 5
CATCA 61100 1.3901289 19.055344 5
TGAAA 80895 1.3842186 9.350995 7
CTTTT 57945 1.3731284 17.158386 4
AAGTT 71395 1.3666064 5.5797524 2
TTTCA 64260 1.3612671 17.834581 5
GTTCC 39265 1.3576077 6.4388833 5
TCTTG 46550 1.3396288 5.135273 7
GTTGT 46015 1.3385359 6.235388 5
TTTCC 46985 1.3376988 8.436279 5
TGTGG 37710 1.3321611 15.624482 5
GTCAC 43040 1.3302962 11.150922 6
TGTTT 55520 1.3298739 12.580875 4
GTTTG 45570 1.3255912 6.630389 5
GTGTG 37360 1.3197967 65.44628 4
TCGGT 37705 1.3177512 7.761593 7
GATTG 50630 1.3165756 10.277941 5
CATGG 42065 1.3142039 11.924143 5
CATCT 51445 1.3093318 13.848732 5
TTTGC 45430 1.3073972 11.514084 5
AGATT 66130 1.2658265 17.118196 4
TCGCT 36455 1.2604506 8.785186 7
GTCCA 40745 1.2593615 7.0742416 6
TTCTC 44200 1.2584078 6.833861 6
ATTGA 65410 1.2520446 5.0493603 6
TTGCA 48635 1.2511837 5.0897226 6
GATCG 39960 1.2484388 15.575624 5
AGTTG 47875 1.244935 6.3937616 5
ATGGC 39725 1.2410969 5.8932266 6
TCTGG 35485 1.2401644 5.829668 7
TGCAA 53345 1.2267988 6.735463 7
GCCAT 39535 1.2219623 5.4706836 8
TGAAC 52870 1.2158748 5.0069914 7
TCTCT 42665 1.2147051 6.1710286 7
CCCAG 32535 1.2081774 6.483982 1
CAGTA 52450 1.206216 31.64174 4
CTTTA 56335 1.1933858 31.509228 4
TCTCC 34875 1.1929361 5.755504 7
GTGCC 28095 1.1796886 10.70991 6
ATGAA 68785 1.1770008 7.6502514 6
CAGTC 38065 1.176527 51.685028 4
GTCGT 33580 1.1735867 7.575369 6
ATCTC 45660 1.1620969 7.182758 6
GTCGA 36790 1.1494011 10.710828 6
CGCTT 33060 1.1430666 6.640928 2
TTTGT 47635 1.141004 9.281505 5
TGTTA 53020 1.1352932 22.93958 4
GCCTT 32735 1.1318296 5.114856 3
GATGC 36000 1.1247196 12.151411 5
TCACC 36455 1.1147242 5.085755 7
TCGCC 26505 1.1010332 7.654329 7
GTGGA 34660 1.0945512 8.391698 6
TCTAC 42955 1.0932517 5.282034 7
GTGGC 25685 1.0901434 7.779607 6
GTTCA 42235 1.0865375 15.008885 5
GTCTC 31310 1.0825595 10.897926 6
GTGAA 46535 1.0817454 10.877633 6
TCGTT 37360 1.0751566 5.58182 7
ATCGG 34070 1.0644222 5.8629274 6
AGTTT 49705 1.0643107 10.281781 3
AAAGT 61975 1.060473 10.581631 1
ATGCT 41140 1.0583674 6.848671 6
TCACT 41575 1.0581292 5.6152463 7
CAGTT 41060 1.0563093 15.782804 4
GTGTA 40620 1.0562772 49.940693 4
ATGGA 44620 1.0372294 6.098237 6
GTCTT 35985 1.0355865 9.517241 6
GTCTG 29590 1.0341402 7.0161138 6
ATCTG 39790 1.0236373 6.4120526 6
GTCAT 39605 1.018878 7.0233183 6
TCTCA 39955 1.0168984 6.9357543 7
CATCG 32400 1.0014312 13.595463 5
ATCTT 47095 0.9976482 7.9301553 6
TCACA 43580 0.991519 8.329361 7
CATCC 32425 0.99149466 8.141293 5
TGGAT 37910 0.98580664 5.2328343 7
ATGCC 31660 0.97855884 7.1192045 6
TATTT 55430 0.9773354 5.215303 7
TTTAC 45920 0.9727573 5.835278 5
TAAGA 56580 0.96815735 5.949125 7
AGTCA 41845 0.962328 15.558378 5
TTCAC 37725 0.96014255 6.997636 6
TTCAG 37150 0.95572084 6.1750317 6
AGATA 55675 0.95267165 30.115591 4
CATGA 41305 0.94990945 9.725382 5
TTTAT 53755 0.94780195 5.6093583 5
TCAAT 49615 0.93955654 9.274323 7
TCGAA 40380 0.9286367 8.43048 7
TAGAA 54255 0.9283736 10.388151 7
GATCT 36020 0.92665035 15.02136 5
TCAGC 29915 0.9246237 8.587793 7
GTCCC 22155 0.92033166 5.9423256 6
GTAGA 39540 0.9191406 9.998404 6
TTTCG 31805 0.9152932 12.965556 5
CCATA 39640 0.9018773 35.827583 4
GTCAG 28690 0.8963392 9.907894 6
TTTAG 41580 0.89033365 9.636668 5
TCTCG 25750 0.8903195 6.3541703 7
ATATT 55935 0.8816368 5.480081 6
TATTC 41595 0.8811376 5.885836 7
TGTGC 25165 0.87949103 17.338867 5
ATCGA 38180 0.87804234 7.672374 6
GTCGG 20505 0.8702897 8.129483 6
GTAAG 37415 0.86974317 6.030604 6
CATGC 28095 0.86837065 12.0065775 5
ATCAT 45830 0.8678802 5.022952 6
TTCGG 24650 0.8614922 5.3891883 6
GTGGG 20060 0.86059874 6.344997 6
GTTCG 24040 0.8401734 10.694843 5
TTTAA 53075 0.83655804 9.577844 5
ATGCA 36140 0.8311276 5.16324 6
GTAAC 35960 0.82698804 6.111135 6
TCTAA 43115 0.8164664 5.142199 7
ATCAC 35215 0.80120105 8.46196 6
TGTAG 30595 0.7955883 14.918778 5
TAAAG 46390 0.79379326 5.0364285 7
TTCGA 30715 0.79017395 6.387103 6
TCGAG 25225 0.7880848 5.4386005 7
GATAT 41115 0.7870022 6.8693867 5
CATGT 30570 0.78644377 9.082684 5
AGTCT 30515 0.78502876 10.367733 5
TTCGC 22700 0.7848643 6.9076085 6
TTAGA 40505 0.7753259 6.5159025 6
TCGAT 29965 0.77087945 5.5012546 7
GTATT 35915 0.7690316 6.5517826 6
TAAAA 60560 0.7627929 8.575092 7
AGTCC 24665 0.7623548 7.0600457 5
GATCC 24650 0.7618912 9.053647 5
GTCTA 29550 0.76020324 7.035794 6
ATCGT 29465 0.7580164 5.651089 6
GTTAA 39410 0.75436604 7.6120243 5
ATCGC 24270 0.75014603 7.7636795 6
AAGTG 32225 0.7490972 5.9192867 2
GATAA 43770 0.7489616 11.236013 5
GTGAC 23885 0.7462203 5.196342 6
AGTGG 23585 0.7448064 9.587214 5
GTGAT 27940 0.7265481 6.582187 6
TGTGT 24950 0.7257736 11.991131 5
TTAAA 51335 0.72331417 5.9032197 6
ATCTA 36785 0.69659555 5.2433376 6
AGTCG 22225 0.69435817 12.105957 5
CATAT 36280 0.6870325 6.612308 5
AGTGC 21910 0.6845169 10.39385 5
TCGCA 21905 0.6770478 6.564492 7
GATAC 29420 0.6765848 6.4352574 5
GTAAA 39390 0.6740141 10.288841 6
ATAAA 53040 0.66807353 6.6574674 6
TAGAG 28525 0.6630876 5.3090534 7
TCGAC 21440 0.6626753 5.35051 7
TAGCC 21175 0.6544846 5.8001328 7
GTTAG 25130 0.6534772 8.373685 5
CATAC 28610 0.6509262 6.0244455 5
AGTGA 27890 0.6483265 9.683871 5
TAGCA 27705 0.63714415 6.2894053 7
TTAGC 24680 0.63491756 6.0253334 6
TGTGA 24260 0.63085383 15.536716 5
GTGCA 20105 0.6281247 7.6808906 6
TCACG 19295 0.5963769 7.973309 7
TGTAC 22385 0.5758765 7.9473486 5
GATAG 24035 0.5587138 12.0512905 5
GTAGG 17690 0.5586443 5.1912146 6
AGTAA 32180 0.55064166 9.1199255 5
ATAGC 23795 0.54722416 6.133438 6
GTGAG 17005 0.5370122 6.278461 6
CATAA 31565 0.5343467 9.6710415 5
ATAGA 30650 0.5244613 8.040232 6
TGTAA 27115 0.51902145 14.277186 5
TGTAT 23685 0.5071562 8.473622 5
AGTAG 21565 0.5012966 9.402036 5
GTAGC 16015 0.50034404 9.695797 6
CATAG 18970 0.43626153 10.439171 5
Produced by FastQC (version 0.10.1)