##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	B80b_GATCAG_L006_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	400514	
Filtered Sequences	0	
Sequence length	101	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.061086503842564	33.0	30.0	34.0	16.0	34.0
2	29.73058369994557	33.0	30.0	34.0	16.0	34.0
3	30.433200836924552	34.0	31.0	34.0	23.0	34.0
4	33.78895868808581	37.0	35.0	37.0	26.0	37.0
5	33.22444408934519	37.0	35.0	37.0	19.0	37.0
6	32.75095502279571	37.0	33.0	37.0	17.0	37.0
7	34.09366713772802	36.0	35.0	37.0	28.0	37.0
8	34.78836195488797	37.0	35.0	37.0	32.0	37.0
9	36.785665419935384	39.0	37.0	39.0	33.0	39.0
10-14	37.22677409528755	39.2	37.2	39.4	33.0	39.4
15-19	38.331259831117016	40.0	38.2	41.0	33.0	41.0
20-24	38.2678333341656	40.0	38.0	41.0	33.0	41.0
25-29	38.11413583545145	40.0	38.0	41.0	33.0	41.0
30-34	37.87644127296424	40.0	38.0	41.0	32.0	41.0
35-39	37.64779058909302	40.0	38.0	41.0	31.8	41.0
40-44	37.52942818478255	40.0	37.6	41.0	31.2	41.0
45-49	37.330653610111014	40.0	37.0	41.0	31.0	41.0
50-54	36.68440853503248	39.2	36.0	40.2	30.4	40.6
55-59	37.16916612153383	39.8	36.2	41.0	31.0	41.0
60-64	36.5753971147076	38.8	35.0	40.6	31.0	41.0
65-69	35.6864509105799	37.0	35.0	39.6	30.0	41.0
70-74	34.661915438661325	35.6	34.0	38.0	29.0	39.6
75-79	33.74339573647862	35.0	33.8	36.4	28.8	38.2
80-84	33.02185142092411	35.0	33.0	35.4	27.8	36.6
85-89	32.57568873996914	35.0	33.0	35.0	27.0	35.8
90-94	32.301281353460794	35.0	33.0	35.0	26.6	35.0
95-99	32.04652821124854	35.0	32.2	35.0	25.2	35.0
100-101	31.404474000908834	34.5	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	19.0
7	285.0
8	794.0
9	695.0
10	535.0
11	523.0
12	539.0
13	664.0
14	810.0
15	896.0
16	931.0
17	1022.0
18	1101.0
19	1219.0
20	1356.0
21	1439.0
22	1696.0
23	1986.0
24	2201.0
25	2522.0
26	3178.0
27	4043.0
28	4969.0
29	5130.0
30	7112.0
31	7858.0
32	8186.0
33	11471.0
34	17228.0
35	28466.0
36	53490.0
37	111830.0
38	108121.0
39	8193.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.910896232069366	22.368342190434333	32.61078035721421	30.109981220282094
2	28.433299288555713	31.585978878919622	18.174167755017443	21.806554077507222
3	16.162771531464934	50.474009400270724	14.795686464509233	18.56753260375511
4	21.30561708524894	19.55054327875157	12.243312964021987	46.9005266719775
5	29.903132280998797	14.395307197279013	37.77566345272611	17.925897068996086
6	35.51347261654169	35.66728842211452	27.89259861316074	0.9266403481830507
7	0.7623028387228868	1.205751853322247	97.3487941233018	0.6831511846530686
8	25.416665626303054	27.046624103310602	13.525559237055587	34.011151033330755
9	26.309310681478266	34.29014449546439	22.52501267175534	16.875532151302007
10-14	19.36122630805127	33.7923398695944	26.43175480231406	20.41467902004027
15-19	21.10290801258086	31.336219318921387	25.46265425762791	22.098218410869848
20-24	20.732069715883352	31.1134873374384	26.41595092698631	21.738492019691936
25-29	21.148895603838607	31.355003278406908	25.587803627171475	21.908297490583006
30-34	21.213437872538282	30.814244140895198	26.317240428684656	21.655077557881867
35-39	21.578393840394796	30.32855489181839	25.889028827455746	22.204022440331073
40-44	21.7773322885312	29.614447036472686	26.418511176935645	22.189709498060473
45-49	21.687428737809576	29.783063284071382	25.874336821507782	22.65517115661126
50-54	22.173375806740005	29.041141867371493	26.334111479706763	22.45137084618174
55-59	22.336695954116383	29.183376129871053	25.960974152755917	22.518953763256647
60-64	21.905500572086662	29.240845239944285	26.26524544790316	22.588408740065894
65-69	22.283763361058476	29.123686368598907	26.064564590061103	22.52798568028152
70-74	22.39292437069641	28.91277975360756	26.23754340735162	22.45675246834441
75-79	22.713014899430096	28.749356922016496	26.146526879411013	22.391101299142395
80-84	22.563040299938113	28.809003737737964	25.84099104251571	22.786964919808213
85-89	22.252772637483112	29.031168915493556	26.27054348939018	22.44551495763315
90-94	22.374477353221803	29.14877886733639	26.313273354880284	22.163470424561527
95-99	22.783134534203626	29.59044165725495	25.665592873302206	21.96083093523922
100-101	22.9923611882929	29.03984744854698	25.92987578345586	22.03791557970426
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	45.020877452351456
2	50.23985336606294
3	34.730304135220045
4	68.20614375722644
5	47.829029349994876
6	36.44011296472474
7	1.4454540233759554
8	59.427816659633805
9	43.18484283278027
10-14	39.77590532809154
15-19	43.2011264234507
20-24	42.47056173557529
25-29	43.05719309442161
30-34	42.86851543042015
35-39	43.78241628072587
40-44	43.96704178659167
45-49	44.342599894420836
50-54	44.62474665292174
55-59	44.85564971737303
60-64	44.49390931215256
65-69	44.81174904134
70-74	44.84967683904082
75-79	45.10411619857249
80-84	45.35000521974632
85-89	44.69828759511626
90-94	44.53794777778333
95-99	44.74396546944284
100-101	45.030276767997165
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	4.0
3	13.0
4	15.0
5	10.5
6	8.0
7	8.0
8	13.0
9	12.0
10	10.0
11	16.5
12	21.0
13	18.5
14	17.5
15	22.5
16	31.0
17	35.5
18	35.0
19	48.0
20	76.0
21	119.5
22	170.0
23	240.0
24	322.5
25	430.0
26	550.5
27	775.0
28	1166.5
29	1607.0
30	1970.0
31	2606.5
32	3647.5
33	4880.5
34	6248.5
35	7570.0
36	9065.0
37	11266.5
38	14066.5
39	17004.5
40	19522.5
41	21760.5
42	24330.5
43	26683.0
44	27711.5
45	26964.5
46	26180.5
47	25172.5
48	23250.0
49	19349.0
50	14208.5
51	10887.0
52	9594.5
53	8773.5
54	7338.5
55	5930.0
56	4348.0
57	3284.5
58	2716.5
59	2060.0
60	1553.0
61	1109.0
62	761.5
63	547.0
64	450.5
65	356.0
66	309.5
67	286.5
68	231.5
69	168.5
70	142.5
71	118.5
72	72.0
73	44.0
74	45.0
75	36.0
76	19.5
77	17.0
78	12.0
79	7.0
80	5.5
81	4.5
82	3.5
83	3.0
84	4.5
85	3.0
86	2.0
87	1.5
88	1.0
89	2.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.020473691306670927
2	0.015230428898864957
3	0.024967916227647474
4	0.018725937170735604
5	0.017727220521629703
6	0.008738770679676615
7	0.00424454575870007
8	0.003245829109594172
9	0.00424454575870007
10-14	0.010087038155969579
15-19	0.02401913541099687
20-24	0.01642888887779204
25-29	0.003645315769236531
30-34	0.01293338060592139
35-39	0.005193326575350674
40-44	0.0138322255901167
45-49	0.01403196891993788
50-54	0.0187758730031909
55-59	0.02302041876189097
60-64	0.012533893946279032
65-69	0.014781006406767305
70-74	0.015829658888328497
75-79	0.023969199578541572
80-84	0.027814258677599287
85-89	0.020723370468947403
90-94	0.03695251601691826
95-99	0.0316093819442017
100-101	0.031085055703421103
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	400514.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	58.21676813192081
#Duplication Level	Relative count
1	100.0
2	30.60590763770976
3	11.91623453107552
4	4.794792957337127
5	2.09566640662047
6	1.0876049745307697
7	0.6026953023419455
8	0.3931286616798984
9	0.2493384118825815
10++	1.222981965031435
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CAGTG	107580	3.3610375	214.94926	2
TGACC	103640	3.203343	173.98749	1
GCTTT	105165	3.026468	157.30902	3
GAAGA	137870	2.864985	5.0585837	9
GACCA	102730	2.8384464	157.45235	2
AAGAA	177590	2.7164946	5.526107	9
TGGTG	76345	2.6969988	6.9889393	7
CTCAG	86925	2.6867096	200.32697	1
TCAGA	113895	2.6192937	148.49788	2
ACAGT	112055	2.5769787	162.04843	1
AGTGT	98260	2.55514	185.84587	3
CAGAT	108895	2.5043068	150.50569	3
CGATG	79975	2.4985962	133.19566	1
TGTTG	84685	2.463412	27.791899	4
CTTTG	84260	2.4248576	42.749718	4
AGCTT	90420	2.3261445	136.75638	2
ACCAT	101835	2.3169193	131.16393	3
TGCTG	66175	2.3127487	9.083437	7
CAAGA	110530	2.2723076	5.5129585	8
TGAAG	95250	2.2141664	7.7888665	7
GATCA	96060	2.2091343	113.538734	1
TTGGT	73640	2.1421227	5.0780253	6
TTTGG	73305	2.1323779	11.328091	5
GTGTC	60755	2.123325	86.052414	4
ATCAG	89395	2.0558565	113.43976	2
TAGCT	78980	2.0318391	136.9044	1
GATGT	77080	2.004378	101.21568	2
TCAAG	86485	1.9889338	14.195767	7
CCATC	64080	1.9594439	61.42453	4
TCAGT	75115	1.9324081	126.88646	3
TTGAA	100370	1.9212309	6.7106547	7
TCCAA	83180	1.8924862	6.575231	7
TGCCA	61090	1.8881923	10.401274	7
CAACA	92145	1.874101	5.148306	8
CTTTC	65065	1.85245	55.63112	4
GTGGT	52100	1.8405088	7.55441	6
GTTGC	52320	1.8285306	8.627061	5
TGGTT	62045	1.8048345	5.2330565	7
ATGTT	83235	1.7822733	83.27204	3
AGATC	77430	1.780692	52.654434	4
TGGCC	42400	1.7803451	5.802754	7
TTGCC	50480	1.7453722	6.3878613	6
TTCTT	73035	1.7307179	6.871606	6
TGTCG	48585	1.6979961	19.93207	5
GTCAA	73340	1.6866326	20.496838	6
ATGGT	64750	1.6837504	10.241387	3
TTGCT	58160	1.6737448	5.9308314	6
TTGGC	47740	1.6684641	5.016351	6
GTTGA	63830	1.6598269	7.894468	5
TTTGA	77225	1.6535839	9.958583	5
TGTTC	57035	1.6413692	39.414043	4
GTTGG	46390	1.6387948	7.7608447	5
TCAAA	96610	1.6354579	15.924652	7
GTGTT	55795	1.6230274	27.227596	4
CCATG	52380	1.6189802	45.749043	4
TGTCT	55865	1.6076988	18.617926	5
TCTTC	55685	1.5853943	8.959726	7
CAAAA	104390	1.5797325	5.738575	8
GTTCT	54890	1.5796399	9.99283	5
TTCTG	54815	1.5774814	5.205177	6
TTTTG	65720	1.574195	8.096631	5
TTCAA	82555	1.5633397	13.76491	6
TTGTT	65235	1.5625778	5.7146273	6
CCATT	61300	1.5601519	18.909786	4
TGCTT	53480	1.5390625	5.232956	7
ATCAA	90550	1.5328714	15.92505	6
AGATG	65725	1.5278329	37.981113	4
TCAAC	67130	1.5273215	11.804525	7
TGTCC	44145	1.5263363	11.888458	5
TGGCT	43600	1.5237755	7.710753	7
TTTCT	63485	1.5044105	13.078204	5
GATGA	64635	1.5024949	11.341064	5
TGGAA	63295	1.4713457	7.4619827	7
ATGTC	56970	1.4656099	5.2893195	6
TCTTT	61410	1.4552388	8.49162	7
TGTCA	55915	1.4384692	26.686623	5
TTGGA	55190	1.4351535	5.2960124	6
GTCGC	34150	1.4339336	11.66688	6
CATTG	55705	1.4330667	8.5337305	5
TCTGC	41230	1.4255487	5.331468	7
GTGCT	40665	1.4212002	9.744602	6
TCCCA	46360	1.4176003	6.331246	7
GATGG	44425	1.4029266	13.247509	5
CATCA	61100	1.3901289	19.055344	5
TGAAA	80895	1.3842186	9.350995	7
CTTTT	57945	1.3731284	17.158386	4
AAGTT	71395	1.3666064	5.5797524	2
TTTCA	64260	1.3612671	17.834581	5
GTTCC	39265	1.3576077	6.4388833	5
TCTTG	46550	1.3396288	5.135273	7
GTTGT	46015	1.3385359	6.235388	5
TTTCC	46985	1.3376988	8.436279	5
TGTGG	37710	1.3321611	15.624482	5
GTCAC	43040	1.3302962	11.150922	6
TGTTT	55520	1.3298739	12.580875	4
GTTTG	45570	1.3255912	6.630389	5
GTGTG	37360	1.3197967	65.44628	4
TCGGT	37705	1.3177512	7.761593	7
GATTG	50630	1.3165756	10.277941	5
CATGG	42065	1.3142039	11.924143	5
CATCT	51445	1.3093318	13.848732	5
TTTGC	45430	1.3073972	11.514084	5
AGATT	66130	1.2658265	17.118196	4
TCGCT	36455	1.2604506	8.785186	7
GTCCA	40745	1.2593615	7.0742416	6
TTCTC	44200	1.2584078	6.833861	6
ATTGA	65410	1.2520446	5.0493603	6
TTGCA	48635	1.2511837	5.0897226	6
GATCG	39960	1.2484388	15.575624	5
AGTTG	47875	1.244935	6.3937616	5
ATGGC	39725	1.2410969	5.8932266	6
TCTGG	35485	1.2401644	5.829668	7
TGCAA	53345	1.2267988	6.735463	7
GCCAT	39535	1.2219623	5.4706836	8
TGAAC	52870	1.2158748	5.0069914	7
TCTCT	42665	1.2147051	6.1710286	7
CCCAG	32535	1.2081774	6.483982	1
CAGTA	52450	1.206216	31.64174	4
CTTTA	56335	1.1933858	31.509228	4
TCTCC	34875	1.1929361	5.755504	7
GTGCC	28095	1.1796886	10.70991	6
ATGAA	68785	1.1770008	7.6502514	6
CAGTC	38065	1.176527	51.685028	4
GTCGT	33580	1.1735867	7.575369	6
ATCTC	45660	1.1620969	7.182758	6
GTCGA	36790	1.1494011	10.710828	6
CGCTT	33060	1.1430666	6.640928	2
TTTGT	47635	1.141004	9.281505	5
TGTTA	53020	1.1352932	22.93958	4
GCCTT	32735	1.1318296	5.114856	3
GATGC	36000	1.1247196	12.151411	5
TCACC	36455	1.1147242	5.085755	7
TCGCC	26505	1.1010332	7.654329	7
GTGGA	34660	1.0945512	8.391698	6
TCTAC	42955	1.0932517	5.282034	7
GTGGC	25685	1.0901434	7.779607	6
GTTCA	42235	1.0865375	15.008885	5
GTCTC	31310	1.0825595	10.897926	6
GTGAA	46535	1.0817454	10.877633	6
TCGTT	37360	1.0751566	5.58182	7
ATCGG	34070	1.0644222	5.8629274	6
AGTTT	49705	1.0643107	10.281781	3
AAAGT	61975	1.060473	10.581631	1
ATGCT	41140	1.0583674	6.848671	6
TCACT	41575	1.0581292	5.6152463	7
CAGTT	41060	1.0563093	15.782804	4
GTGTA	40620	1.0562772	49.940693	4
ATGGA	44620	1.0372294	6.098237	6
GTCTT	35985	1.0355865	9.517241	6
GTCTG	29590	1.0341402	7.0161138	6
ATCTG	39790	1.0236373	6.4120526	6
GTCAT	39605	1.018878	7.0233183	6
TCTCA	39955	1.0168984	6.9357543	7
CATCG	32400	1.0014312	13.595463	5
ATCTT	47095	0.9976482	7.9301553	6
TCACA	43580	0.991519	8.329361	7
CATCC	32425	0.99149466	8.141293	5
TGGAT	37910	0.98580664	5.2328343	7
ATGCC	31660	0.97855884	7.1192045	6
TATTT	55430	0.9773354	5.215303	7
TTTAC	45920	0.9727573	5.835278	5
TAAGA	56580	0.96815735	5.949125	7
AGTCA	41845	0.962328	15.558378	5
TTCAC	37725	0.96014255	6.997636	6
TTCAG	37150	0.95572084	6.1750317	6
AGATA	55675	0.95267165	30.115591	4
CATGA	41305	0.94990945	9.725382	5
TTTAT	53755	0.94780195	5.6093583	5
TCAAT	49615	0.93955654	9.274323	7
TCGAA	40380	0.9286367	8.43048	7
TAGAA	54255	0.9283736	10.388151	7
GATCT	36020	0.92665035	15.02136	5
TCAGC	29915	0.9246237	8.587793	7
GTCCC	22155	0.92033166	5.9423256	6
GTAGA	39540	0.9191406	9.998404	6
TTTCG	31805	0.9152932	12.965556	5
CCATA	39640	0.9018773	35.827583	4
GTCAG	28690	0.8963392	9.907894	6
TTTAG	41580	0.89033365	9.636668	5
TCTCG	25750	0.8903195	6.3541703	7
ATATT	55935	0.8816368	5.480081	6
TATTC	41595	0.8811376	5.885836	7
TGTGC	25165	0.87949103	17.338867	5
ATCGA	38180	0.87804234	7.672374	6
GTCGG	20505	0.8702897	8.129483	6
GTAAG	37415	0.86974317	6.030604	6
CATGC	28095	0.86837065	12.0065775	5
ATCAT	45830	0.8678802	5.022952	6
TTCGG	24650	0.8614922	5.3891883	6
GTGGG	20060	0.86059874	6.344997	6
GTTCG	24040	0.8401734	10.694843	5
TTTAA	53075	0.83655804	9.577844	5
ATGCA	36140	0.8311276	5.16324	6
GTAAC	35960	0.82698804	6.111135	6
TCTAA	43115	0.8164664	5.142199	7
ATCAC	35215	0.80120105	8.46196	6
TGTAG	30595	0.7955883	14.918778	5
TAAAG	46390	0.79379326	5.0364285	7
TTCGA	30715	0.79017395	6.387103	6
TCGAG	25225	0.7880848	5.4386005	7
GATAT	41115	0.7870022	6.8693867	5
CATGT	30570	0.78644377	9.082684	5
AGTCT	30515	0.78502876	10.367733	5
TTCGC	22700	0.7848643	6.9076085	6
TTAGA	40505	0.7753259	6.5159025	6
TCGAT	29965	0.77087945	5.5012546	7
GTATT	35915	0.7690316	6.5517826	6
TAAAA	60560	0.7627929	8.575092	7
AGTCC	24665	0.7623548	7.0600457	5
GATCC	24650	0.7618912	9.053647	5
GTCTA	29550	0.76020324	7.035794	6
ATCGT	29465	0.7580164	5.651089	6
GTTAA	39410	0.75436604	7.6120243	5
ATCGC	24270	0.75014603	7.7636795	6
AAGTG	32225	0.7490972	5.9192867	2
GATAA	43770	0.7489616	11.236013	5
GTGAC	23885	0.7462203	5.196342	6
AGTGG	23585	0.7448064	9.587214	5
GTGAT	27940	0.7265481	6.582187	6
TGTGT	24950	0.7257736	11.991131	5
TTAAA	51335	0.72331417	5.9032197	6
ATCTA	36785	0.69659555	5.2433376	6
AGTCG	22225	0.69435817	12.105957	5
CATAT	36280	0.6870325	6.612308	5
AGTGC	21910	0.6845169	10.39385	5
TCGCA	21905	0.6770478	6.564492	7
GATAC	29420	0.6765848	6.4352574	5
GTAAA	39390	0.6740141	10.288841	6
ATAAA	53040	0.66807353	6.6574674	6
TAGAG	28525	0.6630876	5.3090534	7
TCGAC	21440	0.6626753	5.35051	7
TAGCC	21175	0.6544846	5.8001328	7
GTTAG	25130	0.6534772	8.373685	5
CATAC	28610	0.6509262	6.0244455	5
AGTGA	27890	0.6483265	9.683871	5
TAGCA	27705	0.63714415	6.2894053	7
TTAGC	24680	0.63491756	6.0253334	6
TGTGA	24260	0.63085383	15.536716	5
GTGCA	20105	0.6281247	7.6808906	6
TCACG	19295	0.5963769	7.973309	7
TGTAC	22385	0.5758765	7.9473486	5
GATAG	24035	0.5587138	12.0512905	5
GTAGG	17690	0.5586443	5.1912146	6
AGTAA	32180	0.55064166	9.1199255	5
ATAGC	23795	0.54722416	6.133438	6
GTGAG	17005	0.5370122	6.278461	6
CATAA	31565	0.5343467	9.6710415	5
ATAGA	30650	0.5244613	8.040232	6
TGTAA	27115	0.51902145	14.277186	5
TGTAT	23685	0.5071562	8.473622	5
AGTAG	21565	0.5012966	9.402036	5
GTAGC	16015	0.50034404	9.695797	6
CATAG	18970	0.43626153	10.439171	5
>>END_MODULE