FastQCFastQC Report
Thu 31 Jul 2014
B80b_GATCAG_L006_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename B80b_GATCAG_L006_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 400514
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
ACAGTGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 851 0.21247696709727998 No Hit
CTCAGATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 849 0.21197760877272706 No Hit
TGACCATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 715 0.17852060102767942 No Hit
CGATGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 553 0.1380725767388905 No Hit
TAGCTTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 445 0.11110722721303125 No Hit
ACAGTGTGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAAT 432 0.10786139810343709 No Hit
GATCAGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 412 0.10286781485790758 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CAGTG 143885 4.330026 207.58833 2
GACCA 97020 3.352573 200.8001 2
ACAGT 120470 3.1556423 188.0603 1
TCTTC 147970 3.01921 7.8848963 7
TGACC 99315 3.0154684 173.92046 1
ACCAT 113585 3.0018897 155.15831 3
TTCTT 192365 2.9753468 6.0316834 50-54
TCAGA 110145 2.8851852 167.64673 2
GCAGC 71595 2.8422735 7.372248 8
AGTGT 119440 2.7246878 163.63698 3
AGCTT 117930 2.7142894 119.382034 2
CTCAG 89355 2.713056 201.02515 1
CAGAT 101315 2.6538885 169.11375 3
CACCA 75460 2.6308658 6.108144 40-44
ATCAG 92885 2.4330692 125.92239 2
GATCA 90485 2.3702028 125.66992 1
TCTTG 109155 2.2074883 6.295175 7
CGATG 73115 2.200298 132.10464 1
TGTTG 109715 2.1991549 41.13002 4
TCAGT 93035 2.1413033 111.454025 3
GCTTT 103560 2.0943382 109.57996 3
CCATG 68205 2.0708857 86.60883 4
TAGCT 88140 2.0286396 119.294235 1
GCGGC 44240 2.0177362 35.31606 8
GGCAG 51080 2.0098753 10.11142 8
CTTTG 99005 2.0022204 55.784576 4
TGGCA 66005 1.986332 14.780954 7
TCAGC 65115 1.9770651 6.0947833 7
AGATG 75625 1.9634029 78.22376 4
GATGT 85725 1.9555749 92.30781 2
TTTCT 125495 1.9410558 11.42181 5
TTGGC 71555 1.8920734 24.295465 6
CCTTC 65940 1.7749116 5.16822 9
CCATC 57335 1.7564049 57.23047 4
TTTGG 86960 1.7430483 18.87394 5
CGGCA 43490 1.7265236 19.364182 9
AGATC 65545 1.7169137 55.40105 4
CATCG 56265 1.7083554 14.38328 5
GATGG 57060 1.7019309 28.374786 5
GTTGG 64770 1.697489 16.913542 5
GTGTC 63765 1.6860882 66.451164 4
GGAGG 43000 1.6769565 5.367307 75-79
AGCGG 41520 1.6337123 8.890418 8
TGCTG 61495 1.6260645 13.154166 7
AGTGG 54065 1.6125989 18.800827 5
ACACC 46245 1.6123029 5.5264153 40-44
GTGTG 61430 1.6099544 86.74068 4
TGTTC 79185 1.6013919 25.661491 4
CTTTC 78315 1.5979552 35.95241 4
ATGTT 90025 1.5706769 70.94266 3
TCTGG 59330 1.5688171 14.551637 7
TCCTT 76575 1.562452 5.569883 7
ATGGC 51890 1.56156 31.911102 6
GCCTT 58230 1.5534942 6.76523 8
TTCTG 76565 1.5484067 7.1972876 6
CTTTT 99910 1.5453273 7.319557 4
GGTCA 51010 1.5350777 5.6242385 60-64
TCTCT 74665 1.5234798 11.763038 7
CATGG 49820 1.4992661 25.111887 5
CAGTC 49370 1.4990048 49.33303 4
GTGGA 50085 1.4938872 5.2352414 6
GTCTT 73515 1.4867252 6.569731 6
TGGCG 42935 1.4844058 29.754835 7
GTTCT 73130 1.478939 8.276002 5
GTGGC 42360 1.464526 33.409233 6
GGCGG 31650 1.4307314 15.60567 8
TGGCC 41005 1.4303519 40.574608 7
TTCTC 69980 1.4278862 11.080406 6
TGTCC 53200 1.4193007 24.137812 5
GTTTC 69485 1.4052247 5.6480775 4
GTAGC 46455 1.398001 9.6886015 6
TGGTC 51610 1.364683 5.0001216 7
GTTGT 67705 1.357096 9.213436 5
CGGCG 29565 1.3484261 13.577996 8
AAAGT 59475 1.3440404 14.749371 1
TTTCC 65280 1.3319865 12.999855 5
ACCGG 33455 1.3281409 5.639866 8
AGCCT 43655 1.3254822 6.0509963 2
GATGA 50580 1.3131756 6.48298 5
GGCCA 33000 1.3100777 8.796651 8
TGTGG 49335 1.2929692 24.576101 5
ATCTT 73185 1.2882812 5.7697015 6
GCGGT 37015 1.2797318 7.627296 8
TTGCT 62875 1.271548 8.217067 6
CAGTT 54880 1.2631239 9.340728 4
CCGGC 27345 1.2583228 17.893963 9
TTTGC 61970 1.253246 25.230045 5
TTGCC 46715 1.2462903 23.063873 6
GATGC 41100 1.2368494 36.113842 5
TTGGG 47105 1.2345256 10.127667 6
CATCT 53140 1.2340088 15.301698 5
CTCTG 45995 1.2270818 5.8209443 8
TTGCA 53230 1.2251472 5.133431 6
TGCCC 34735 1.2224702 46.38082 7
CAGTA 46630 1.2214462 14.402749 4
GTTGC 46040 1.2173998 23.129055 5
AGTAG 46555 1.2086773 5.224149 5
GTCAG 39755 1.1963735 5.0048046 6
TGTCT 58635 1.1858006 17.650242 5
CCCTT 43910 1.1819285 5.5858536 9
AAGTT 59425 1.1799675 6.633843 2
TGGCT 44300 1.1713904 11.65413 7
ATCGG 38605 1.1617658 14.693406 6
TTGTC 56990 1.1525333 12.168712 6
TGTGC 43105 1.139792 39.61684 5
GTGAC 37815 1.1379918 5.1507173 6
TGCTT 55940 1.1312985 5.618591 7
ATGGT 49570 1.1308001 7.57681 3
TGCGG 32675 1.1296835 41.23769 7
TGCAG 37525 1.1292646 11.118546 7
GCTGC 32310 1.1270497 5.5305953 8
AGTTT 64500 1.1253393 11.192122 3
TACCG 36925 1.1211414 7.9202743 7
CCCAG 27915 1.1181129 6.4287505 1
GCCCC 23995 1.1140376 16.973385 8
GAGAT 42820 1.1117078 5.0903816 95-97
CCATT 47575 1.1047792 12.38548 4
CCATA 41700 1.1020716 17.132812 4
ATCTG 47850 1.1013206 9.641922 6
TCTGC 41230 1.0999582 9.248833 7
GTTTG 54840 1.0992267 7.230798 4
GCCTC 31100 1.0945393 7.883716 8
GCCCA 27295 1.0932794 11.924342 8
AAGTG 41680 1.0821108 6.432948 2
TTTGT 70530 1.0812362 11.030725 5
TCCAG 35595 1.0807592 15.428341 9
GCGGA 27445 1.0798948 10.512061 9
GATCT 46770 1.0764632 18.279705 5
AGGGG 27510 1.0728623 5.401349 75-79
ATGGG 35610 1.0621408 12.683168 6
TGTAG 46525 1.0613369 6.3378944 5
ATTGC 46090 1.0608124 5.5909758 6
CTGGC 30285 1.0564129 6.883646 8
GCCAG 26565 1.0546126 5.3511357 9
GGGCA 26690 1.0501873 7.3260098 8
TGTCA 45550 1.0483835 6.3498297 7
GGGTC 30320 1.0482633 5.091957 60-64
TAGCC 34475 1.0467529 6.713598 1
GTGCC 29830 1.0405413 41.910744 6
TGCCA 33760 1.0250436 9.053845 7
GGCGA 25920 1.0198897 5.551742 8
TTGTG 50745 1.0171456 7.0557723 6
TCTCC 37670 1.013966 25.149391 7
AGATA 44785 1.0120697 17.400103 4
AGTCC 33270 1.0101659 16.4149 5
GTGTT 50365 1.0095286 12.012454 4
CCCGG 21790 1.0027009 12.338356 8
ATGTC 43550 1.0023514 14.753034 6
CTCCA 32565 0.99759877 15.18007 8
GATCG 33145 0.9974544 15.569078 5
CCGCC 21290 0.98845005 7.991447 9
GGCCT 28275 0.9862992 10.858232 8
CATTG 42715 0.98313296 5.6356845 5
GGCCC 21310 0.98061293 22.423233 8
TGACG 32485 0.9775926 5.3549953 7
GTCTG 36940 0.9767757 11.615667 6
GTCCC 27680 0.97417516 25.11868 6
TCCGG 27865 0.97199744 22.44373 7
TTAGC 42105 0.96909314 6.160117 6
GTGTA 42470 0.9688335 17.785923 4
CGCCA 24155 0.96750915 5.1659203 9
CATGT 41870 0.9636843 14.775537 5
CATCC 31435 0.9629823 19.101578 5
TGGGC 27775 0.9602741 26.016623 7
GGCGT 27730 0.95871836 7.7614026 8
GCCCT 27235 0.95851374 13.412556 8
CGGCC 20775 0.95599407 9.348592 9
GTTCC 35630 0.950558 13.039075 5
CCTCC 26755 0.95003766 5.1134067 9
TGCCG 27170 0.9477542 28.887632 7
TCGGC 27060 0.9439172 28.10963 7
CCGGG 20625 0.9406829 7.29762 8
GATAG 36205 0.9399669 7.45228 5
CCGGT 26935 0.93955684 6.20712 9
ATAGC 35785 0.93736744 7.912522 6
GTCTC 34845 0.92961544 17.734474 6
GCGCC 20045 0.92240196 12.8738365 8
GTGCT 34855 0.9216436 10.538718 6
CGCCC 19710 0.9150941 5.111326 70-74
GTCCT 34050 0.90840596 7.179164 6
GATTG 39795 0.90781105 5.795911 5
GCCTG 25990 0.90659297 6.7821665 8
AGATT 45635 0.9061475 11.32222 4
TCCCC 25400 0.90192324 16.338799 7
CCTCG 25540 0.89885956 5.7486696 85-89
CGGCT 25665 0.8952562 10.012575 9
TGTGT 44650 0.8949758 15.5209465 5
GTGCG 25775 0.89112747 28.832853 6
ATGCC 29275 0.8888671 32.358295 6
TGGGG 25840 0.88545966 5.2668815 7
TGCTC 33135 0.88399506 10.012023 7
GCCGG 19345 0.8823036 17.492176 8
TGTGA 38675 0.8822613 5.4530225 5
GTCGG 25420 0.878854 19.043095 6
GATCC 28890 0.8771775 19.786211 5
TAGCG 29125 0.87647784 12.898681 7
AGTCT 38035 0.8754176 12.0190735 5
TTTCG 43195 0.873551 10.011612 5
TTGCG 32900 0.8699491 18.769688 6
CATGC 28590 0.86806864 38.644943 5
ATCCT 37065 0.8607177 6.0575504 6
GTACC 28330 0.86017436 5.1525946 6
CTTTA 48555 0.85471743 11.821207 4
ATGCA 32510 0.8515808 6.6932573 6
AGTGC 28235 0.8496945 30.75878 5
TTCCC 31120 0.8376591 14.499727 6
CCCGC 17985 0.83500594 10.872871 8
TGCCT 31165 0.83143824 18.782246 7
TTCGG 31400 0.8302858 11.33361 6
GTGGG 24155 0.82771975 13.59088 6
ATCTC 35570 0.82600105 16.100924 6
CGCTT 30510 0.8139637 6.455183 2
TGTCG 30505 0.80661994 16.398039 5
GCCGC 17300 0.7960866 10.464176 8
GCGTG 22900 0.7917292 5.3307257 8
TGCGC 22400 0.78136533 21.53042 7
CATAG 29815 0.78098685 6.0455985 5
CGAGA 22650 0.7757474 5.585845 95-97
ATGCT 33655 0.774607 10.222223 6
TCCCG 21830 0.76828915 25.067486 7
TGTTA 43800 0.7641837 8.281897 4
TCCTG 28530 0.76114017 6.6100054 7
CCCCT 21345 0.7579351 5.802081 9
CCCCG 16240 0.75398916 8.104003 8
TCGGT 28490 0.753339 5.2821803 7
GGCGC 16510 0.7530024 7.010138 8
TCCCA 24410 0.7477779 6.0304384 7
GCGCT 21335 0.7442155 6.545383 8
ATCCG 24500 0.74388534 11.2326565 6
ATGTG 32485 0.74105394 8.892851 6
GGGCC 16035 0.73133814 16.740297 8
TCCCT 27025 0.7274338 13.285978 7
TCGCC 20635 0.7262321 12.525211 7
CCCTG 20485 0.720953 6.0407696 8
ATCCC 23130 0.70856625 18.492355 6
CCGTG 20040 0.6990428 5.15857 4
CGGGC 15155 0.69120234 7.29762 8
GTTCG 26020 0.6880266 9.025973 5
CCGCA 17175 0.68793076 7.107995 9
TTCGC 25725 0.6863067 7.1015515 6
AGTCG 22710 0.68342704 13.686781 5
GCCCG 14815 0.6817354 12.717655 8
CCCAC 16790 0.6785215 5.62358 9
GCCGT 19430 0.67776465 6.021076 8
TGCGT 25255 0.6677984 9.92332 7
TTCCG 24820 0.66216254 9.1841545 6
TCCGC 18575 0.65373206 10.562815 7
ATGCG 21600 0.65002304 24.017227 6
TCTCG 23805 0.63508373 7.321453 7
CTCCC 17240 0.61217153 6.9728274 8
GTGCA 20245 0.60924613 7.310225 6
GTCCG 16910 0.589861 15.932172 6
GGCCG 12515 0.57079494 6.678428 8
TCGCT 20935 0.55851626 7.269712 7
TCGGG 14845 0.51324105 11.834882 7
CCCCC 10930 0.51199317 5.5418124 9
GTCGC 14600 0.50928277 13.310637 6
TCGCG 14300 0.49881798 9.505181 7
ATCGC 16395 0.49779594 10.172694 6
CCCGT 14130 0.4972939 6.0919623 8
Produced by FastQC (version 0.10.1)