##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	B80b_GATCAG_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	400514	
Filtered Sequences	0	
Sequence length	101	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.69309687052138	33.0	31.0	34.0	25.0	34.0
2	30.452241369839754	34.0	31.0	34.0	16.0	34.0
3	31.20432244565733	34.0	31.0	34.0	25.0	34.0
4	35.26691950843166	37.0	35.0	37.0	32.0	37.0
5	34.28782015110583	37.0	35.0	37.0	28.0	37.0
6	32.76546637570721	35.0	33.0	37.0	17.0	37.0
7	34.626871969519165	35.0	35.0	37.0	32.0	37.0
8	34.99753316987671	36.0	35.0	37.0	32.0	37.0
9	36.64036463144859	39.0	37.0	39.0	32.0	39.0
10-14	37.81516750974997	39.2	37.6	39.4	34.8	39.4
15-19	39.25646294511552	40.0	39.0	41.0	36.0	41.0
20-24	39.17866092071688	40.0	39.0	41.0	36.0	41.0
25-29	39.019259751219685	40.0	39.0	41.0	36.0	41.0
30-34	38.81629506084681	40.0	38.6	41.0	35.2	41.0
35-39	38.59652396670278	40.0	38.0	41.0	34.8	41.0
40-44	38.30428699121629	40.0	38.0	41.0	34.0	41.0
45-49	38.11726581342974	40.0	38.0	41.0	33.4	41.0
50-54	37.97735809484812	40.0	37.2	41.0	33.4	41.0
55-59	37.441393808955496	39.4	36.2	40.8	32.8	41.0
60-64	36.69722456643213	38.2	35.0	40.0	31.8	41.0
65-69	35.787213430741495	36.6	34.8	39.4	31.0	40.8
70-74	35.105726641265974	35.6	34.0	38.0	31.0	39.6
75-79	34.0231202904268	34.8	33.4	36.2	30.0	38.2
80-84	33.8966787677834	35.0	34.0	35.6	30.8	36.8
85-89	33.513900138322256	35.0	34.0	35.0	30.0	36.0
90-94	33.253137218674006	35.0	33.8	35.0	30.0	35.4
95-99	33.09566656845953	35.0	33.4	35.0	29.2	35.0
100-101	32.80446501245899	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	21.0
9	79.0
10	53.0
11	27.0
12	26.0
13	30.0
14	49.0
15	77.0
16	107.0
17	194.0
18	361.0
19	494.0
20	601.0
21	795.0
22	1036.0
23	1355.0
24	1633.0
25	2007.0
26	2443.0
27	3022.0
28	3440.0
29	4284.0
30	5313.0
31	6637.0
32	8784.0
33	12085.0
34	18327.0
35	31095.0
36	61303.0
37	119755.0
38	105859.0
39	9219.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.80352354271846	22.189180686431666	32.73234358536452	30.27495218548536
2	28.489990786079737	31.135809189449688	18.288258369310903	22.085941655159672
3	16.036223633748804	50.44343240651965	14.877956216043465	18.64238774368808
4	22.320817749192287	18.838542472922295	12.636761761136938	46.203878016748476
5	30.039125017789775	13.944186301999196	37.717316128645656	18.299372551565376
6	35.00910082818785	34.48352487934224	29.585057164186928	0.9223171282829771
7	0.6926099961549409	0.6541594051643638	97.9908317811612	0.6623988175194874
8	47.157402737482336	11.690228056946824	8.891324647827542	32.261044557743304
9	29.26714172288609	9.620637480837125	26.14190764867146	34.970313147605324
10-14	26.756617746196138	18.117369180602925	26.036992464682886	29.089020608518055
15-19	21.632440271094623	25.422861274735986	32.7679536270791	20.17674482709029
20-24	21.248558088437242	26.935357419290405	31.416593842850364	20.399490649421985
25-29	21.76947296290303	28.00252920270181	29.80483585154819	20.42316198284697
30-34	22.145741203614687	27.111369691642718	29.941594648381802	20.80129445636079
35-39	21.953709938795452	27.070124467232688	29.581199433313415	21.394966160658445
40-44	21.285003546559835	27.175289968730333	29.66702298770193	21.872683497007902
45-49	21.589825976968775	27.02961928340637	29.47546313225471	21.905091607370153
50-54	21.261514990905898	27.073847302016592	29.453199969827864	22.21143773724965
55-59	21.4816631706307	26.38502221260861	29.56742237326474	22.56589224349595
60-64	22.508430376332	26.274298218005786	29.02542351712803	22.191847888534188
65-69	22.11198631471558	26.943453276084828	28.67524728137868	22.269313127820915
70-74	22.222860382013547	26.054332924633606	28.768232294603415	22.95457439874943
75-79	21.943650379852535	26.850270381490603	28.05639956131677	23.149679677340092
80-84	23.43781522844262	26.173024335386085	28.781642693491587	21.607517742679708
85-89	21.959642348466538	26.464395654006502	28.77567673926636	22.8002852582606
90-94	22.423203009351283	26.1809965779217	29.153290329282644	22.242510083444373
95-99	22.293185115271005	27.363011902889205	28.08078084151704	22.263022140322754
100-101	22.827369322558454	26.830745107047576	28.853469230298668	21.488416340095306
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	45.07847572820382
2	50.57593244123942
3	34.67861137743688
4	68.52469576594076
5	48.33849756935515
6	35.931417956470824
7	1.3550088136744285
8	79.41844729522563
9	64.23745487049142
10-14	55.84563835471419
15-19	41.80918509818491
20-24	41.64804873785923
25-29	42.19263494575
30-34	42.94703565997548
35-39	43.34867609945389
40-44	43.15768704356774
45-49	43.49491758433893
50-54	43.47295272815555
55-59	44.04755541412665
60-64	44.70027826486619
65-69	44.381299442536495
70-74	45.17743478076298
75-79	45.093330057192624
80-84	45.04533297112233
85-89	44.75992760672714
90-94	44.665713092795656
95-99	44.55620725559376
100-101	44.31578566265376
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	3.5
3	10.0
4	12.5
5	8.5
6	5.0
7	3.0
8	6.0
9	9.0
10	9.0
11	7.0
12	6.0
13	6.5
14	7.5
15	17.0
16	29.5
17	56.5
18	148.5
19	306.0
20	310.0
21	257.0
22	342.5
23	446.5
24	617.5
25	762.0
26	813.5
27	1014.0
28	1273.0
29	1530.0
30	2093.0
31	2463.5
32	2702.5
33	3369.5
34	4190.5
35	5281.0
36	6714.5
37	8790.5
38	11236.0
39	13086.0
40	15423.5
41	17638.5
42	19541.5
43	22842.5
44	26226.0
45	29616.0
46	31015.0
47	30053.0
48	27006.5
49	21268.0
50	16790.0
51	14587.5
52	12706.5
53	10601.5
54	8383.0
55	6410.5
56	5042.5
57	4484.5
58	3603.0
59	2381.0
60	1693.0
61	1321.0
62	1015.0
63	619.0
64	491.0
65	385.0
66	316.5
67	256.0
68	212.5
69	167.5
70	126.5
71	101.5
72	86.0
73	56.5
74	34.0
75	33.5
76	17.5
77	5.5
78	5.5
79	4.0
80	1.0
81	1.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001997433298211798
2	0.008239412355123666
3	4.993583245529495E-4
4	0.0
5	7.490374868294241E-4
6	2.4967916227647475E-4
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.001098588314016489
20-24	9.98716649105899E-4
25-29	0.01787702801899559
30-34	0.009238129004229566
35-39	0.03785136100111357
40-44	0.031459574446835815
45-49	0.03825084766075593
50-54	0.036103606865178245
55-59	0.03890001348267476
60-64	0.043943532560659554
65-69	0.05013557578511613
70-74	0.01423171224975906
75-79	0.01068626814543312
80-84	0.0026965349525859272
85-89	0.00868883484722132
90-94	0.013882161422571995
95-99	0.005742620732358919
100-101	0.003620347853008884
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	400514.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	63.85926585214553
#Duplication Level	Relative count
1	100.0
2	26.581541653295922
3	10.742617482220016
4	4.632870394555184
5	2.3007634385331235
6	1.2559795222352406
7	0.7932895021604639
8	0.5268696035045037
9	0.3582116284449884
10++	2.0627019604623915
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACAGTGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA	851	0.21247696709727998	No Hit
CTCAGATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA	849	0.21197760877272706	No Hit
TGACCATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA	715	0.17852060102767942	No Hit
CGATGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA	553	0.1380725767388905	No Hit
TAGCTTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA	445	0.11110722721303125	No Hit
ACAGTGTGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAAT	432	0.10786139810343709	No Hit
GATCAGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA	412	0.10286781485790758	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CAGTG	143885	4.330026	207.58833	2
GACCA	97020	3.352573	200.8001	2
ACAGT	120470	3.1556423	188.0603	1
TCTTC	147970	3.01921	7.8848963	7
TGACC	99315	3.0154684	173.92046	1
ACCAT	113585	3.0018897	155.15831	3
TTCTT	192365	2.9753468	6.0316834	50-54
TCAGA	110145	2.8851852	167.64673	2
GCAGC	71595	2.8422735	7.372248	8
AGTGT	119440	2.7246878	163.63698	3
AGCTT	117930	2.7142894	119.382034	2
CTCAG	89355	2.713056	201.02515	1
CAGAT	101315	2.6538885	169.11375	3
CACCA	75460	2.6308658	6.108144	40-44
ATCAG	92885	2.4330692	125.92239	2
GATCA	90485	2.3702028	125.66992	1
TCTTG	109155	2.2074883	6.295175	7
CGATG	73115	2.200298	132.10464	1
TGTTG	109715	2.1991549	41.13002	4
TCAGT	93035	2.1413033	111.454025	3
GCTTT	103560	2.0943382	109.57996	3
CCATG	68205	2.0708857	86.60883	4
TAGCT	88140	2.0286396	119.294235	1
GCGGC	44240	2.0177362	35.31606	8
GGCAG	51080	2.0098753	10.11142	8
CTTTG	99005	2.0022204	55.784576	4
TGGCA	66005	1.986332	14.780954	7
TCAGC	65115	1.9770651	6.0947833	7
AGATG	75625	1.9634029	78.22376	4
GATGT	85725	1.9555749	92.30781	2
TTTCT	125495	1.9410558	11.42181	5
TTGGC	71555	1.8920734	24.295465	6
CCTTC	65940	1.7749116	5.16822	9
CCATC	57335	1.7564049	57.23047	4
TTTGG	86960	1.7430483	18.87394	5
CGGCA	43490	1.7265236	19.364182	9
AGATC	65545	1.7169137	55.40105	4
CATCG	56265	1.7083554	14.38328	5
GATGG	57060	1.7019309	28.374786	5
GTTGG	64770	1.697489	16.913542	5
GTGTC	63765	1.6860882	66.451164	4
GGAGG	43000	1.6769565	5.367307	75-79
AGCGG	41520	1.6337123	8.890418	8
TGCTG	61495	1.6260645	13.154166	7
AGTGG	54065	1.6125989	18.800827	5
ACACC	46245	1.6123029	5.5264153	40-44
GTGTG	61430	1.6099544	86.74068	4
TGTTC	79185	1.6013919	25.661491	4
CTTTC	78315	1.5979552	35.95241	4
ATGTT	90025	1.5706769	70.94266	3
TCTGG	59330	1.5688171	14.551637	7
TCCTT	76575	1.562452	5.569883	7
ATGGC	51890	1.56156	31.911102	6
GCCTT	58230	1.5534942	6.76523	8
TTCTG	76565	1.5484067	7.1972876	6
CTTTT	99910	1.5453273	7.319557	4
GGTCA	51010	1.5350777	5.6242385	60-64
TCTCT	74665	1.5234798	11.763038	7
CATGG	49820	1.4992661	25.111887	5
CAGTC	49370	1.4990048	49.33303	4
GTGGA	50085	1.4938872	5.2352414	6
GTCTT	73515	1.4867252	6.569731	6
TGGCG	42935	1.4844058	29.754835	7
GTTCT	73130	1.478939	8.276002	5
GTGGC	42360	1.464526	33.409233	6
GGCGG	31650	1.4307314	15.60567	8
TGGCC	41005	1.4303519	40.574608	7
TTCTC	69980	1.4278862	11.080406	6
TGTCC	53200	1.4193007	24.137812	5
GTTTC	69485	1.4052247	5.6480775	4
GTAGC	46455	1.398001	9.6886015	6
TGGTC	51610	1.364683	5.0001216	7
GTTGT	67705	1.357096	9.213436	5
CGGCG	29565	1.3484261	13.577996	8
AAAGT	59475	1.3440404	14.749371	1
TTTCC	65280	1.3319865	12.999855	5
ACCGG	33455	1.3281409	5.639866	8
AGCCT	43655	1.3254822	6.0509963	2
GATGA	50580	1.3131756	6.48298	5
GGCCA	33000	1.3100777	8.796651	8
TGTGG	49335	1.2929692	24.576101	5
ATCTT	73185	1.2882812	5.7697015	6
GCGGT	37015	1.2797318	7.627296	8
TTGCT	62875	1.271548	8.217067	6
CAGTT	54880	1.2631239	9.340728	4
CCGGC	27345	1.2583228	17.893963	9
TTTGC	61970	1.253246	25.230045	5
TTGCC	46715	1.2462903	23.063873	6
GATGC	41100	1.2368494	36.113842	5
TTGGG	47105	1.2345256	10.127667	6
CATCT	53140	1.2340088	15.301698	5
CTCTG	45995	1.2270818	5.8209443	8
TTGCA	53230	1.2251472	5.133431	6
TGCCC	34735	1.2224702	46.38082	7
CAGTA	46630	1.2214462	14.402749	4
GTTGC	46040	1.2173998	23.129055	5
AGTAG	46555	1.2086773	5.224149	5
GTCAG	39755	1.1963735	5.0048046	6
TGTCT	58635	1.1858006	17.650242	5
CCCTT	43910	1.1819285	5.5858536	9
AAGTT	59425	1.1799675	6.633843	2
TGGCT	44300	1.1713904	11.65413	7
ATCGG	38605	1.1617658	14.693406	6
TTGTC	56990	1.1525333	12.168712	6
TGTGC	43105	1.139792	39.61684	5
GTGAC	37815	1.1379918	5.1507173	6
TGCTT	55940	1.1312985	5.618591	7
ATGGT	49570	1.1308001	7.57681	3
TGCGG	32675	1.1296835	41.23769	7
TGCAG	37525	1.1292646	11.118546	7
GCTGC	32310	1.1270497	5.5305953	8
AGTTT	64500	1.1253393	11.192122	3
TACCG	36925	1.1211414	7.9202743	7
CCCAG	27915	1.1181129	6.4287505	1
GCCCC	23995	1.1140376	16.973385	8
GAGAT	42820	1.1117078	5.0903816	95-97
CCATT	47575	1.1047792	12.38548	4
CCATA	41700	1.1020716	17.132812	4
ATCTG	47850	1.1013206	9.641922	6
TCTGC	41230	1.0999582	9.248833	7
GTTTG	54840	1.0992267	7.230798	4
GCCTC	31100	1.0945393	7.883716	8
GCCCA	27295	1.0932794	11.924342	8
AAGTG	41680	1.0821108	6.432948	2
TTTGT	70530	1.0812362	11.030725	5
TCCAG	35595	1.0807592	15.428341	9
GCGGA	27445	1.0798948	10.512061	9
GATCT	46770	1.0764632	18.279705	5
AGGGG	27510	1.0728623	5.401349	75-79
ATGGG	35610	1.0621408	12.683168	6
TGTAG	46525	1.0613369	6.3378944	5
ATTGC	46090	1.0608124	5.5909758	6
CTGGC	30285	1.0564129	6.883646	8
GCCAG	26565	1.0546126	5.3511357	9
GGGCA	26690	1.0501873	7.3260098	8
TGTCA	45550	1.0483835	6.3498297	7
GGGTC	30320	1.0482633	5.091957	60-64
TAGCC	34475	1.0467529	6.713598	1
GTGCC	29830	1.0405413	41.910744	6
TGCCA	33760	1.0250436	9.053845	7
GGCGA	25920	1.0198897	5.551742	8
TTGTG	50745	1.0171456	7.0557723	6
TCTCC	37670	1.013966	25.149391	7
AGATA	44785	1.0120697	17.400103	4
AGTCC	33270	1.0101659	16.4149	5
GTGTT	50365	1.0095286	12.012454	4
CCCGG	21790	1.0027009	12.338356	8
ATGTC	43550	1.0023514	14.753034	6
CTCCA	32565	0.99759877	15.18007	8
GATCG	33145	0.9974544	15.569078	5
CCGCC	21290	0.98845005	7.991447	9
GGCCT	28275	0.9862992	10.858232	8
CATTG	42715	0.98313296	5.6356845	5
GGCCC	21310	0.98061293	22.423233	8
TGACG	32485	0.9775926	5.3549953	7
GTCTG	36940	0.9767757	11.615667	6
GTCCC	27680	0.97417516	25.11868	6
TCCGG	27865	0.97199744	22.44373	7
TTAGC	42105	0.96909314	6.160117	6
GTGTA	42470	0.9688335	17.785923	4
CGCCA	24155	0.96750915	5.1659203	9
CATGT	41870	0.9636843	14.775537	5
CATCC	31435	0.9629823	19.101578	5
TGGGC	27775	0.9602741	26.016623	7
GGCGT	27730	0.95871836	7.7614026	8
GCCCT	27235	0.95851374	13.412556	8
CGGCC	20775	0.95599407	9.348592	9
GTTCC	35630	0.950558	13.039075	5
CCTCC	26755	0.95003766	5.1134067	9
TGCCG	27170	0.9477542	28.887632	7
TCGGC	27060	0.9439172	28.10963	7
CCGGG	20625	0.9406829	7.29762	8
GATAG	36205	0.9399669	7.45228	5
CCGGT	26935	0.93955684	6.20712	9
ATAGC	35785	0.93736744	7.912522	6
GTCTC	34845	0.92961544	17.734474	6
GCGCC	20045	0.92240196	12.8738365	8
GTGCT	34855	0.9216436	10.538718	6
CGCCC	19710	0.9150941	5.111326	70-74
GTCCT	34050	0.90840596	7.179164	6
GATTG	39795	0.90781105	5.795911	5
GCCTG	25990	0.90659297	6.7821665	8
AGATT	45635	0.9061475	11.32222	4
TCCCC	25400	0.90192324	16.338799	7
CCTCG	25540	0.89885956	5.7486696	85-89
CGGCT	25665	0.8952562	10.012575	9
TGTGT	44650	0.8949758	15.5209465	5
GTGCG	25775	0.89112747	28.832853	6
ATGCC	29275	0.8888671	32.358295	6
TGGGG	25840	0.88545966	5.2668815	7
TGCTC	33135	0.88399506	10.012023	7
GCCGG	19345	0.8823036	17.492176	8
TGTGA	38675	0.8822613	5.4530225	5
GTCGG	25420	0.878854	19.043095	6
GATCC	28890	0.8771775	19.786211	5
TAGCG	29125	0.87647784	12.898681	7
AGTCT	38035	0.8754176	12.0190735	5
TTTCG	43195	0.873551	10.011612	5
TTGCG	32900	0.8699491	18.769688	6
CATGC	28590	0.86806864	38.644943	5
ATCCT	37065	0.8607177	6.0575504	6
GTACC	28330	0.86017436	5.1525946	6
CTTTA	48555	0.85471743	11.821207	4
ATGCA	32510	0.8515808	6.6932573	6
AGTGC	28235	0.8496945	30.75878	5
TTCCC	31120	0.8376591	14.499727	6
CCCGC	17985	0.83500594	10.872871	8
TGCCT	31165	0.83143824	18.782246	7
TTCGG	31400	0.8302858	11.33361	6
GTGGG	24155	0.82771975	13.59088	6
ATCTC	35570	0.82600105	16.100924	6
CGCTT	30510	0.8139637	6.455183	2
TGTCG	30505	0.80661994	16.398039	5
GCCGC	17300	0.7960866	10.464176	8
GCGTG	22900	0.7917292	5.3307257	8
TGCGC	22400	0.78136533	21.53042	7
CATAG	29815	0.78098685	6.0455985	5
CGAGA	22650	0.7757474	5.585845	95-97
ATGCT	33655	0.774607	10.222223	6
TCCCG	21830	0.76828915	25.067486	7
TGTTA	43800	0.7641837	8.281897	4
TCCTG	28530	0.76114017	6.6100054	7
CCCCT	21345	0.7579351	5.802081	9
CCCCG	16240	0.75398916	8.104003	8
TCGGT	28490	0.753339	5.2821803	7
GGCGC	16510	0.7530024	7.010138	8
TCCCA	24410	0.7477779	6.0304384	7
GCGCT	21335	0.7442155	6.545383	8
ATCCG	24500	0.74388534	11.2326565	6
ATGTG	32485	0.74105394	8.892851	6
GGGCC	16035	0.73133814	16.740297	8
TCCCT	27025	0.7274338	13.285978	7
TCGCC	20635	0.7262321	12.525211	7
CCCTG	20485	0.720953	6.0407696	8
ATCCC	23130	0.70856625	18.492355	6
CCGTG	20040	0.6990428	5.15857	4
CGGGC	15155	0.69120234	7.29762	8
GTTCG	26020	0.6880266	9.025973	5
CCGCA	17175	0.68793076	7.107995	9
TTCGC	25725	0.6863067	7.1015515	6
AGTCG	22710	0.68342704	13.686781	5
GCCCG	14815	0.6817354	12.717655	8
CCCAC	16790	0.6785215	5.62358	9
GCCGT	19430	0.67776465	6.021076	8
TGCGT	25255	0.6677984	9.92332	7
TTCCG	24820	0.66216254	9.1841545	6
TCCGC	18575	0.65373206	10.562815	7
ATGCG	21600	0.65002304	24.017227	6
TCTCG	23805	0.63508373	7.321453	7
CTCCC	17240	0.61217153	6.9728274	8
GTGCA	20245	0.60924613	7.310225	6
GTCCG	16910	0.589861	15.932172	6
GGCCG	12515	0.57079494	6.678428	8
TCGCT	20935	0.55851626	7.269712	7
TCGGG	14845	0.51324105	11.834882	7
CCCCC	10930	0.51199317	5.5418124	9
GTCGC	14600	0.50928277	13.310637	6
TCGCG	14300	0.49881798	9.505181	7
ATCGC	16395	0.49779594	10.172694	6
CCCGT	14130	0.4972939	6.0919623	8
>>END_MODULE