FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | B80a_ACAGTG_L006_R2_001.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 387699 |
| Filtered Sequences | 0 |
| Sequence length | 101 |
| %GC | 43 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
No overrepresented sequences
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| TGACC | 105700 | 3.3592901 | 192.22531 | 1 |
| CTCAG | 101115 | 3.213573 | 251.27556 | 1 |
| TCAGA | 127015 | 3.0215995 | 187.21288 | 2 |
| GACCA | 105265 | 2.9954715 | 174.06343 | 2 |
| CAGAT | 123250 | 2.9320323 | 189.76273 | 3 |
| CAGTG | 90560 | 2.9224877 | 170.44588 | 2 |
| TGGTG | 74415 | 2.7234027 | 7.27564 | 7 |
| AAGAA | 170255 | 2.7145617 | 5.411841 | 9 |
| GCTTT | 90255 | 2.678156 | 125.43658 | 3 |
| CGATG | 78920 | 2.54685 | 142.99115 | 1 |
| TGTTG | 83440 | 2.5141008 | 31.124495 | 4 |
| ACCAT | 104735 | 2.4537477 | 144.83998 | 3 |
| CTTTG | 80015 | 2.3743021 | 34.19207 | 4 |
| TGCTG | 64825 | 2.336416 | 8.615699 | 7 |
| CAAGA | 107135 | 2.2820327 | 5.845936 | 8 |
| GATCA | 95480 | 2.2714033 | 117.095955 | 1 |
| TGAAG | 93215 | 2.2517045 | 8.491796 | 7 |
| ACAGT | 93465 | 2.2234678 | 128.72594 | 1 |
| AGTGT | 80430 | 2.1698766 | 148.06496 | 3 |
| TTTGG | 70275 | 2.1174307 | 8.883904 | 5 |
| GATGT | 78280 | 2.111873 | 108.71966 | 2 |
| ATCAG | 88075 | 2.0952437 | 116.77441 | 2 |
| CCATC | 65190 | 2.040374 | 68.639435 | 4 |
| TCAAG | 85040 | 2.0230432 | 14.441828 | 7 |
| AGCTT | 76125 | 2.022556 | 109.42729 | 2 |
| TCAGT | 75370 | 2.0024965 | 130.49577 | 3 |
| TTGAA | 98880 | 1.9664865 | 6.711539 | 6 |
| GTGTC | 53520 | 1.9289622 | 67.59214 | 4 |
| AGATC | 79830 | 1.8991005 | 66.02676 | 4 |
| TCCAA | 80365 | 1.8828038 | 6.1456995 | 7 |
| TGCCA | 58665 | 1.8644537 | 11.86586 | 7 |
| GTTGC | 51555 | 1.85814 | 9.071246 | 5 |
| ATGTT | 83540 | 1.8555318 | 89.55176 | 3 |
| GTGGT | 50530 | 1.8492715 | 7.4884887 | 6 |
| TGGTT | 60025 | 1.8085916 | 5.3179674 | 7 |
| TAGCT | 65670 | 1.7447783 | 109.4774 | 1 |
| TTGCC | 49090 | 1.7424362 | 5.438527 | 6 |
| ATGGT | 63875 | 1.7232484 | 11.422827 | 3 |
| TTCTT | 70185 | 1.7146119 | 6.3610106 | 6 |
| GTTGG | 46185 | 1.6902554 | 9.015858 | 5 |
| CTTTC | 57750 | 1.6876129 | 43.59348 | 4 |
| GTCAA | 70790 | 1.6840452 | 18.086655 | 6 |
| GTTGA | 62345 | 1.6819714 | 9.341296 | 5 |
| TGGTC | 46510 | 1.6763086 | 5.453516 | 4 |
| TGTCG | 46265 | 1.6674783 | 16.010138 | 5 |
| ATTGG | 61700 | 1.6645703 | 5.808049 | 6 |
| AGATG | 68720 | 1.6600025 | 47.843803 | 4 |
| CCATG | 52060 | 1.6545378 | 50.986168 | 4 |
| TTGCT | 55450 | 1.6453798 | 5.654412 | 6 |
| TGTTC | 55360 | 1.6427091 | 41.142735 | 4 |
| TTTGA | 73185 | 1.6255338 | 8.04612 | 5 |
| TCAAA | 92265 | 1.6180222 | 15.807486 | 7 |
| TTCTG | 53315 | 1.5820274 | 5.5249214 | 6 |
| CCATT | 60285 | 1.5773889 | 21.902044 | 4 |
| TGTCT | 52970 | 1.5717902 | 14.620098 | 5 |
| TCTTC | 53555 | 1.5650234 | 8.756796 | 7 |
| GTGTT | 51920 | 1.5643828 | 21.655634 | 4 |
| GTTCT | 52675 | 1.5630366 | 10.763616 | 5 |
| TTCAA | 79735 | 1.5616647 | 11.785307 | 6 |
| CAAAA | 99230 | 1.5581133 | 5.66469 | 8 |
| ATCAA | 88050 | 1.544105 | 19.063972 | 6 |
| TTTTG | 61930 | 1.5362656 | 6.676521 | 5 |
| TCAAC | 64775 | 1.5175589 | 11.234929 | 7 |
| GATGA | 62540 | 1.5107182 | 14.596059 | 5 |
| TGTCC | 42105 | 1.4945056 | 9.243365 | 5 |
| TTTCT | 61035 | 1.4910784 | 11.207393 | 5 |
| TGCTT | 50185 | 1.4891503 | 5.5393815 | 7 |
| TGGCT | 41155 | 1.4833043 | 7.2875175 | 7 |
| CATTG | 55505 | 1.4747056 | 9.5602455 | 5 |
| ATGTC | 55225 | 1.4672663 | 5.6941094 | 6 |
| TGGAA | 60305 | 1.4567294 | 8.327816 | 7 |
| TCTTT | 59505 | 1.4537007 | 8.363001 | 7 |
| GATGG | 44295 | 1.4514922 | 16.463104 | 5 |
| GTCGC | 33450 | 1.4421256 | 10.703042 | 6 |
| GTGCT | 39925 | 1.4389727 | 9.052484 | 6 |
| TCTGC | 40310 | 1.4307926 | 5.455808 | 7 |
| CATCA | 60815 | 1.4247832 | 21.893328 | 5 |
| TCCCA | 43875 | 1.3732384 | 6.070503 | 7 |
| GTTGT | 45510 | 1.3712454 | 6.911327 | 5 |
| TGTCA | 51505 | 1.3684301 | 20.460472 | 5 |
| TGAAA | 76545 | 1.3630382 | 9.290507 | 7 |
| CTTTT | 55680 | 1.3602563 | 14.217299 | 4 |
| GTTTG | 44590 | 1.3435253 | 7.042832 | 5 |
| TGTTT | 54125 | 1.3426512 | 13.00485 | 4 |
| ATTGC | 50485 | 1.3413298 | 5.5395184 | 6 |
| GTTCC | 37530 | 1.3321172 | 6.66142 | 5 |
| GATTG | 49300 | 1.3300375 | 12.899885 | 5 |
| TCTTG | 44335 | 1.315562 | 5.366725 | 7 |
| TCGGT | 36485 | 1.3149886 | 7.1651855 | 7 |
| ATTGA | 65980 | 1.3121845 | 5.6218786 | 6 |
| CATGG | 40640 | 1.311505 | 12.081629 | 5 |
| TTTCA | 59915 | 1.3105866 | 13.556667 | 5 |
| CATCT | 49885 | 1.3052672 | 15.087013 | 5 |
| AGATT | 65610 | 1.3048259 | 22.260387 | 4 |
| TTTCC | 44420 | 1.2980738 | 6.561359 | 5 |
| TTTGC | 43435 | 1.288856 | 8.878544 | 5 |
| GATCG | 39910 | 1.287947 | 19.296179 | 5 |
| TCGCT | 36250 | 1.286684 | 9.792917 | 7 |
| GTCAC | 40400 | 1.2839671 | 9.369286 | 6 |
| TGTGG | 34885 | 1.2767037 | 12.423427 | 5 |
| AGTTG | 47110 | 1.2709547 | 7.758247 | 5 |
| TCTGA | 47725 | 1.2679998 | 5.320583 | 7 |
| TCTGG | 35010 | 1.2618268 | 5.8370047 | 7 |
| TGCAA | 52730 | 1.2544103 | 6.528813 | 7 |
| ATGGC | 38600 | 1.2456716 | 6.3372617 | 6 |
| TGAAC | 52215 | 1.2421588 | 5.3637776 | 7 |
| TGCTC | 34895 | 1.2385886 | 5.3525434 | 7 |
| GCCAT | 38825 | 1.2339115 | 6.318348 | 8 |
| CCCAG | 32365 | 1.2304032 | 7.872932 | 1 |
| GTCCA | 38700 | 1.2299387 | 5.516783 | 6 |
| CAGTA | 51505 | 1.2252684 | 31.542398 | 4 |
| TTCTC | 41725 | 1.2193185 | 6.3478866 | 6 |
| CAGTC | 38360 | 1.2191331 | 52.727837 | 4 |
| ATGAA | 66670 | 1.1871939 | 9.221313 | 6 |
| GTCGT | 32875 | 1.1848773 | 7.51461 | 6 |
| TCTCT | 40170 | 1.1738772 | 5.696168 | 7 |
| GATGC | 36355 | 1.1732225 | 15.868876 | 5 |
| TTTGT | 47175 | 1.1702461 | 7.747171 | 5 |
| GTGCC | 27040 | 1.1657721 | 10.075911 | 6 |
| GTGTG | 31800 | 1.1638005 | 51.77286 | 4 |
| TCGTC | 32615 | 1.1576606 | 5.1460147 | 7 |
| TCTCC | 33095 | 1.1568646 | 6.2035055 | 7 |
| ATCTC | 44165 | 1.1556005 | 9.439147 | 6 |
| GTCGA | 35665 | 1.1509554 | 9.842315 | 6 |
| TGTTA | 51815 | 1.1508784 | 24.796684 | 4 |
| GCCTT | 31825 | 1.1296198 | 5.57765 | 3 |
| ATCCA | 47535 | 1.1136575 | 6.3614554 | 6 |
| CTTTA | 50170 | 1.0974234 | 24.780916 | 4 |
| ATCGG | 33980 | 1.0965784 | 6.5406804 | 6 |
| TCGTT | 36890 | 1.0946449 | 6.100514 | 7 |
| GATTT | 49180 | 1.0923516 | 6.1503806 | 5 |
| GTGGA | 33290 | 1.090872 | 7.753721 | 6 |
| GTTCA | 41045 | 1.0905197 | 15.345354 | 5 |
| TCTAC | 41355 | 1.0820754 | 5.1763725 | 7 |
| TCGCC | 25320 | 1.0750457 | 7.905519 | 7 |
| GTGGC | 24480 | 1.0716726 | 6.325536 | 6 |
| ATGCT | 40300 | 1.0707258 | 8.244864 | 6 |
| GTGAA | 44325 | 1.070716 | 10.30715 | 6 |
| GTCTG | 29430 | 1.060713 | 6.5709147 | 6 |
| TCACT | 40395 | 1.0569565 | 5.924917 | 7 |
| GTCTC | 29555 | 1.0490468 | 9.310896 | 6 |
| ATCTG | 39420 | 1.0473453 | 7.3559656 | 6 |
| ATGGA | 43245 | 1.0446275 | 7.671799 | 6 |
| AGATA | 58550 | 1.0426009 | 38.170498 | 4 |
| GTCTT | 34815 | 1.033073 | 8.63269 | 6 |
| AAAGT | 57705 | 1.0275539 | 8.169944 | 1 |
| TCCGT | 28915 | 1.0263301 | 5.9047346 | 3 |
| CAGTT | 38470 | 1.0221047 | 16.41561 | 4 |
| CATCG | 31995 | 1.0168446 | 14.949828 | 5 |
| AGTTT | 45710 | 1.0152785 | 8.003964 | 3 |
| GTCAT | 38205 | 1.015064 | 6.106353 | 6 |
| TCTCA | 38525 | 1.0080268 | 7.5235023 | 7 |
| ATCTT | 46010 | 1.0064274 | 8.994073 | 6 |
| TTCAG | 37875 | 1.0062963 | 5.771405 | 6 |
| GTTAC | 37715 | 1.0020453 | 5.1666555 | 5 |
| GTGTA | 36530 | 0.98552275 | 40.20628 | 4 |
| TATTT | 53810 | 0.9839972 | 5.1516094 | 7 |
| TCACA | 41795 | 0.9791798 | 8.020081 | 7 |
| ATGGG | 29845 | 0.97798365 | 5.036741 | 6 |
| AGTCA | 40875 | 0.972388 | 15.389681 | 5 |
| ATGCC | 30575 | 0.9717152 | 9.477156 | 6 |
| CATCC | 30985 | 0.96979576 | 9.425673 | 5 |
| TAAGA | 54185 | 0.9648732 | 6.0871816 | 7 |
| GATCT | 35950 | 0.9551512 | 18.978764 | 5 |
| TCAGC | 29995 | 0.9532821 | 8.306102 | 7 |
| CATGA | 40065 | 0.9531187 | 11.236544 | 5 |
| TTCAC | 36415 | 0.9528176 | 6.216642 | 6 |
| TCAAT | 48535 | 0.9505914 | 9.154862 | 7 |
| TCGAA | 39775 | 0.9462198 | 9.066514 | 7 |
| ATATT | 56310 | 0.92198753 | 6.1369042 | 6 |
| TAGAA | 51675 | 0.92017764 | 9.402753 | 7 |
| ATCCC | 29285 | 0.9165877 | 5.3419743 | 6 |
| GTCAG | 27995 | 0.90343463 | 8.887814 | 6 |
| TATTC | 41300 | 0.9034003 | 5.7062187 | 7 |
| CCATA | 38425 | 0.90022695 | 37.210354 | 4 |
| GTAGA | 36975 | 0.8931692 | 8.608813 | 6 |
| GTTAT | 39900 | 0.88623077 | 5.2348247 | 5 |
| GTAAG | 36685 | 0.886164 | 5.2355638 | 6 |
| CATGC | 27830 | 0.8844753 | 13.901799 | 5 |
| ATCAT | 45075 | 0.8828249 | 5.73596 | 6 |
| TCTCG | 24870 | 0.88275397 | 6.5400853 | 7 |
| GTCCC | 20705 | 0.8791003 | 5.537909 | 6 |
| ATCGA | 36795 | 0.87532777 | 8.512725 | 6 |
| TTTCG | 29235 | 0.86749643 | 10.288749 | 5 |
| GTCGG | 19795 | 0.866575 | 7.3444133 | 6 |
| TTTAG | 38900 | 0.86401945 | 7.561414 | 5 |
| ATGAT | 42915 | 0.8534767 | 5.1011553 | 6 |
| ATGCA | 35590 | 0.8466615 | 6.251892 | 6 |
| GTTCG | 23255 | 0.8381543 | 11.553167 | 5 |
| TGTGC | 23190 | 0.83581156 | 14.157436 | 5 |
| GTGGG | 18665 | 0.8297027 | 5.5177846 | 6 |
| GTAAC | 34105 | 0.8113344 | 5.8135676 | 6 |
| ATCAC | 34375 | 0.8053429 | 9.621701 | 6 |
| TTTAA | 48855 | 0.7999236 | 7.352625 | 5 |
| AGTCT | 30105 | 0.7998561 | 10.526578 | 5 |
| ATCGT | 30090 | 0.7994576 | 6.6603055 | 6 |
| CATGT | 29995 | 0.79693353 | 10.436387 | 5 |
| GATAT | 39940 | 0.79431105 | 8.622079 | 5 |
| GATCC | 24880 | 0.79072034 | 12.715061 | 5 |
| TTCGA | 29690 | 0.78883004 | 6.518596 | 6 |
| GATAA | 44270 | 0.78831667 | 14.775192 | 5 |
| TCGAG | 24415 | 0.7879034 | 5.195057 | 7 |
| AGTAC | 32870 | 0.7819546 | 5.2260313 | 5 |
| TTCGC | 21905 | 0.777512 | 6.6604743 | 6 |
| GTTAA | 38815 | 0.77193755 | 8.48706 | 5 |
| AGTGG | 23500 | 0.77006584 | 10.3609495 | 5 |
| GTATT | 34430 | 0.764735 | 5.8264165 | 6 |
| TTAGA | 38420 | 0.76408195 | 5.795453 | 6 |
| ATCGC | 24025 | 0.76354736 | 9.677485 | 6 |
| TCGAT | 28490 | 0.75694734 | 5.1531067 | 7 |
| GTCTA | 28445 | 0.7557518 | 6.2867093 | 6 |
| AGTAT | 37930 | 0.75433695 | 5.044013 | 5 |
| AGTCC | 23670 | 0.7522649 | 7.182083 | 5 |
| TAAAA | 56685 | 0.744089 | 8.344405 | 7 |
| AAGTG | 30775 | 0.7434018 | 5.0494637 | 2 |
| TGTAG | 27350 | 0.73786056 | 12.1410675 | 5 |
| GTGAT | 27035 | 0.72936237 | 5.6903186 | 6 |
| AGTCG | 22390 | 0.7225541 | 13.411861 | 5 |
| TTAAA | 48960 | 0.717777 | 5.5446362 | 6 |
| ATCTA | 36545 | 0.715759 | 5.811933 | 6 |
| TGTGT | 23525 | 0.70882326 | 9.263808 | 5 |
| GATAC | 28920 | 0.68798685 | 8.629296 | 5 |
| AGTGC | 21225 | 0.68495804 | 10.67315 | 5 |
| CATAT | 34740 | 0.68040675 | 6.6485786 | 5 |
| ATAAA | 51610 | 0.67747086 | 8.217001 | 6 |
| AGTGA | 27930 | 0.67467797 | 10.156326 | 5 |
| TAGCC | 21175 | 0.6729704 | 6.1501 | 1 |
| CATAC | 28715 | 0.67273945 | 6.6123075 | 5 |
| TCGAC | 20695 | 0.6577153 | 5.624726 | 7 |
| TCGCA | 20685 | 0.6573975 | 6.302775 | 7 |
| GTAAA | 36510 | 0.6501342 | 8.755067 | 6 |
| GTTAG | 23890 | 0.64451516 | 8.333901 | 5 |
| TAGCA | 26400 | 0.62803775 | 5.6752224 | 7 |
| ATAAC | 35715 | 0.6263226 | 5.033841 | 6 |
| GTGCA | 19250 | 0.62122226 | 6.775393 | 6 |
| TCACG | 18930 | 0.6016212 | 7.3660793 | 7 |
| TAAAC | 33435 | 0.586339 | 5.0168996 | 7 |
| TGTGA | 21700 | 0.58543235 | 12.232649 | 5 |
| TGTAC | 21680 | 0.5760133 | 6.08145 | 5 |
| ATAAG | 32190 | 0.57320786 | 5.2236843 | 6 |
| GATAG | 23185 | 0.5600576 | 14.022057 | 5 |
| ATAGC | 23290 | 0.554053 | 6.79403 | 6 |
| AGTAA | 30540 | 0.5438263 | 8.868569 | 5 |
| CATAA | 30865 | 0.54126966 | 10.392274 | 5 |
| GTGAG | 15950 | 0.52266175 | 5.4021826 | 6 |
| ATAGA | 29240 | 0.5206772 | 8.530579 | 6 |
| TGTAA | 25780 | 0.5127026 | 11.785439 | 5 |
| GTAGC | 15485 | 0.49972084 | 8.825224 | 6 |
| AGTAG | 20680 | 0.4995467 | 8.973179 | 5 |
| TGTAT | 22470 | 0.49908784 | 6.5273743 | 5 |
| CATAG | 18095 | 0.43046755 | 10.498208 | 5 |