##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	B80a_ACAGTG_L006_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	387699	
Filtered Sequences	0	
Sequence length	101	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.094547574277982	33.0	30.0	34.0	16.0	34.0
2	29.770115476181264	33.0	30.0	34.0	16.0	34.0
3	30.517981733251826	34.0	31.0	34.0	24.0	34.0
4	33.81241891261004	37.0	35.0	37.0	26.0	37.0
5	33.19398038168786	37.0	35.0	37.0	19.0	37.0
6	32.76113686132799	37.0	33.0	37.0	17.0	37.0
7	34.17771002762452	36.0	35.0	37.0	28.0	37.0
8	34.84718299505544	37.0	35.0	37.0	32.0	37.0
9	36.83812442126495	39.0	37.0	39.0	33.0	39.0
10-14	37.25692147774433	39.2	37.2	39.4	33.0	39.4
15-19	38.36650597499606	40.0	38.2	41.0	33.0	41.0
20-24	38.30483184119639	40.0	38.0	41.0	33.0	41.0
25-29	38.153779091511716	40.0	38.0	41.0	33.0	41.0
30-34	37.92804830551536	40.0	38.0	41.0	32.2	41.0
35-39	37.70897216655188	40.0	38.0	41.0	31.8	41.0
40-44	37.57980185659493	40.0	38.0	41.0	31.6	41.0
45-49	37.38029605441335	40.0	37.0	41.0	31.0	41.0
50-54	36.72794822787781	39.2	36.2	40.2	30.6	40.6
55-59	37.21564357916837	39.8	36.2	41.0	31.4	41.0
60-64	36.61501164563231	38.8	35.0	40.6	31.0	41.0
65-69	35.73533591781253	37.0	35.0	39.6	30.0	41.0
70-74	34.71579240596442	35.6	34.0	38.0	29.2	39.8
75-79	33.78760017436207	35.0	33.8	36.4	29.0	38.2
80-84	33.072154944944394	35.0	33.0	35.4	28.0	36.6
85-89	32.62476766770097	35.0	33.0	35.0	27.2	36.0
90-94	32.35178682431474	35.0	33.0	35.0	26.8	35.0
95-99	32.095848067702	35.0	32.2	35.0	25.4	35.0
100-101	31.450451252131167	34.5	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	12.0
7	281.0
8	798.0
9	669.0
10	469.0
11	414.0
12	496.0
13	594.0
14	774.0
15	818.0
16	836.0
17	910.0
18	1017.0
19	1072.0
20	1323.0
21	1479.0
22	1633.0
23	1799.0
24	2064.0
25	2480.0
26	2952.0
27	3819.0
28	4830.0
29	5074.0
30	6950.0
31	7457.0
32	7823.0
33	11004.0
34	16499.0
35	27563.0
36	51807.0
37	107974.0
38	105635.0
39	8364.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.264950208967546	18.100459212630927	30.889788968577474	35.74480160982406
2	30.826460565316875	28.588307222404236	22.57268967936828	18.012542532910604
3	13.176178596030455	48.70575669309419	15.03272695374883	23.085337757126528
4	17.100378460514467	24.11002443095482	13.266756616609438	45.52284049192127
5	35.50265328287323	14.810916511236144	30.40453212187817	19.281898084012454
6	32.107812790193776	41.4786211023752	25.547369941600117	0.8661961658309085
7	0.6992772501457365	1.1658919847505456	97.46444287387216	0.6703878912315637
8	25.25549026789722	27.050241690440203	13.624428016487569	34.06984002517501
9	26.233478812641227	34.13114804841056	22.56992808576057	17.06544505318765
10-14	19.323333290340887	33.7255620187533	26.48420672487844	20.46689796602737
15-19	21.025599482365497	31.3586306214452	25.501806715729803	22.113963180459503
20-24	20.69634281492427	31.095566429265737	26.468724783460218	21.739365972349773
25-29	21.159793668178025	31.333898395145354	25.5990024922218	21.90730544445482
30-34	21.22155489665683	30.794154111103257	26.299380214430435	21.68491077780948
35-39	21.574855550969872	30.313815517952953	25.91023524556335	22.201093685513825
40-44	21.76017384942646	29.660736682817152	26.27641006300081	22.30267940475558
45-49	21.62333675569979	29.86152314224834	25.839090304040287	22.67604979801159
50-54	22.19869441297084	29.06396468352001	26.328340897317627	22.40900000619153
55-59	22.383589083124228	29.19304013824369	25.906966208417114	22.516404570214966
60-64	21.926924525478906	29.096875048367878	26.346948567537034	22.62925185861619
65-69	22.31654187785831	29.03702801763278	26.14115719262329	22.50527291188562
70-74	22.32243558191681	28.833793038688516	26.34756614133334	22.496205238061336
75-79	22.718334386811314	28.725274441764164	26.268598186297908	22.28779298512661
80-84	22.537887290438867	28.823134506653787	25.900721340778432	22.738256862128914
85-89	22.158074189959816	29.03317351650007	26.318695267206454	22.49005702633366
90-94	22.438373894456102	29.181043424948932	26.232083454178955	22.14849922641601
95-99	22.83745987842259	29.519521534063347	25.670636681700433	21.972381905813627
100-101	23.06763440832471	28.964519866139966	25.960796658143455	22.007049067391872
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	51.00975181879161
2	48.83900309822749
3	36.261516353156985
4	62.62321895243574
5	54.784551366885694
6	32.97400895602468
7	1.3696651413773002
8	59.325330293072234
9	43.29892386582887
10-14	39.79023125636826
15-19	43.139562662825
20-24	42.435708787274045
25-29	43.067099112632846
30-34	42.90646567446631
35-39	43.7759492364837
40-44	44.06285325418204
45-49	44.29938655371138
50-54	44.60769441916237
55-59	44.899993653339195
60-64	44.556176384095096
65-69	44.82181478974393
70-74	44.81864081997814
75-79	45.00612737193793
80-84	45.27614415256778
85-89	44.64813121629347
90-94	44.58687312087211
95-99	44.80984178423622
100-101	45.07468347571658
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.5
2	4.0
3	11.0
4	14.0
5	8.5
6	9.0
7	14.5
8	16.0
9	12.5
10	10.5
11	13.0
12	14.0
13	15.0
14	18.0
15	17.5
16	25.5
17	37.5
18	44.5
19	55.5
20	76.0
21	111.5
22	172.0
23	234.5
24	289.5
25	359.0
26	524.0
27	756.5
28	1097.0
29	1487.0
30	1943.0
31	2619.0
32	3351.5
33	4550.5
34	5966.5
35	7279.0
36	8891.5
37	11034.5
38	13660.5
39	16328.5
40	18622.5
41	20973.5
42	23592.0
43	25692.5
44	26618.5
45	26185.0
46	25626.5
47	24331.0
48	22121.5
49	18693.5
50	14121.5
51	10722.0
52	9228.0
53	8418.5
54	7215.0
55	5898.0
56	4396.5
57	3415.0
58	2704.0
59	2000.0
60	1507.5
61	1054.0
62	747.0
63	543.0
64	440.0
65	352.5
66	284.0
67	240.5
68	204.5
69	161.5
70	121.0
71	95.5
72	83.0
73	63.5
74	42.5
75	27.5
76	16.0
77	11.0
78	9.5
79	9.5
80	9.5
81	5.0
82	2.5
83	2.5
84	2.5
85	2.0
86	1.0
87	2.0
88	2.0
89	1.5
90	1.5
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.020376632387496485
2	0.014960059221199952
3	0.02527734144271716
4	0.019602836220882696
5	0.017023515665503395
6	0.006964165499524116
7	0.0033531167219930922
8	0.0033531167219930922
9	0.003868980833068953
10-14	0.008769689888289627
15-19	0.023110712176198547
20-24	0.01459895434344685
25-29	0.0033015303108855066
30-34	0.012587084310250993
35-39	0.004900709055220674
40-44	0.013721985354617886
45-49	0.0136703989435103
50-54	0.01872586723205373
55-59	0.024142440398350267
60-64	0.011606942499206858
65-69	0.014805299987877192
70-74	0.015321164098953054
75-79	0.024245613220565437
80-84	0.029713772797969558
85-89	0.022852780120660617
90-94	0.03925725885287298
95-99	0.032757371053317136
100-101	0.03353116721993093
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	387699.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	57.737286700055286
#Duplication Level	Relative count
1	100.0
2	29.757547341036965
3	11.53654183556174
4	4.575291911186399
5	2.04715953778208
6	0.9498457256942344
7	0.6012160445277996
8	0.37131707907314415
9	0.25712384294270674
10++	1.1532760602577288
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGACC	105700	3.3592901	192.22531	1
CTCAG	101115	3.213573	251.27556	1
TCAGA	127015	3.0215995	187.21288	2
GACCA	105265	2.9954715	174.06343	2
CAGAT	123250	2.9320323	189.76273	3
CAGTG	90560	2.9224877	170.44588	2
TGGTG	74415	2.7234027	7.27564	7
AAGAA	170255	2.7145617	5.411841	9
GCTTT	90255	2.678156	125.43658	3
CGATG	78920	2.54685	142.99115	1
TGTTG	83440	2.5141008	31.124495	4
ACCAT	104735	2.4537477	144.83998	3
CTTTG	80015	2.3743021	34.19207	4
TGCTG	64825	2.336416	8.615699	7
CAAGA	107135	2.2820327	5.845936	8
GATCA	95480	2.2714033	117.095955	1
TGAAG	93215	2.2517045	8.491796	7
ACAGT	93465	2.2234678	128.72594	1
AGTGT	80430	2.1698766	148.06496	3
TTTGG	70275	2.1174307	8.883904	5
GATGT	78280	2.111873	108.71966	2
ATCAG	88075	2.0952437	116.77441	2
CCATC	65190	2.040374	68.639435	4
TCAAG	85040	2.0230432	14.441828	7
AGCTT	76125	2.022556	109.42729	2
TCAGT	75370	2.0024965	130.49577	3
TTGAA	98880	1.9664865	6.711539	6
GTGTC	53520	1.9289622	67.59214	4
AGATC	79830	1.8991005	66.02676	4
TCCAA	80365	1.8828038	6.1456995	7
TGCCA	58665	1.8644537	11.86586	7
GTTGC	51555	1.85814	9.071246	5
ATGTT	83540	1.8555318	89.55176	3
GTGGT	50530	1.8492715	7.4884887	6
TGGTT	60025	1.8085916	5.3179674	7
TAGCT	65670	1.7447783	109.4774	1
TTGCC	49090	1.7424362	5.438527	6
ATGGT	63875	1.7232484	11.422827	3
TTCTT	70185	1.7146119	6.3610106	6
GTTGG	46185	1.6902554	9.015858	5
CTTTC	57750	1.6876129	43.59348	4
GTCAA	70790	1.6840452	18.086655	6
GTTGA	62345	1.6819714	9.341296	5
TGGTC	46510	1.6763086	5.453516	4
TGTCG	46265	1.6674783	16.010138	5
ATTGG	61700	1.6645703	5.808049	6
AGATG	68720	1.6600025	47.843803	4
CCATG	52060	1.6545378	50.986168	4
TTGCT	55450	1.6453798	5.654412	6
TGTTC	55360	1.6427091	41.142735	4
TTTGA	73185	1.6255338	8.04612	5
TCAAA	92265	1.6180222	15.807486	7
TTCTG	53315	1.5820274	5.5249214	6
CCATT	60285	1.5773889	21.902044	4
TGTCT	52970	1.5717902	14.620098	5
TCTTC	53555	1.5650234	8.756796	7
GTGTT	51920	1.5643828	21.655634	4
GTTCT	52675	1.5630366	10.763616	5
TTCAA	79735	1.5616647	11.785307	6
CAAAA	99230	1.5581133	5.66469	8
ATCAA	88050	1.544105	19.063972	6
TTTTG	61930	1.5362656	6.676521	5
TCAAC	64775	1.5175589	11.234929	7
GATGA	62540	1.5107182	14.596059	5
TGTCC	42105	1.4945056	9.243365	5
TTTCT	61035	1.4910784	11.207393	5
TGCTT	50185	1.4891503	5.5393815	7
TGGCT	41155	1.4833043	7.2875175	7
CATTG	55505	1.4747056	9.5602455	5
ATGTC	55225	1.4672663	5.6941094	6
TGGAA	60305	1.4567294	8.327816	7
TCTTT	59505	1.4537007	8.363001	7
GATGG	44295	1.4514922	16.463104	5
GTCGC	33450	1.4421256	10.703042	6
GTGCT	39925	1.4389727	9.052484	6
TCTGC	40310	1.4307926	5.455808	7
CATCA	60815	1.4247832	21.893328	5
TCCCA	43875	1.3732384	6.070503	7
GTTGT	45510	1.3712454	6.911327	5
TGTCA	51505	1.3684301	20.460472	5
TGAAA	76545	1.3630382	9.290507	7
CTTTT	55680	1.3602563	14.217299	4
GTTTG	44590	1.3435253	7.042832	5
TGTTT	54125	1.3426512	13.00485	4
ATTGC	50485	1.3413298	5.5395184	6
GTTCC	37530	1.3321172	6.66142	5
GATTG	49300	1.3300375	12.899885	5
TCTTG	44335	1.315562	5.366725	7
TCGGT	36485	1.3149886	7.1651855	7
ATTGA	65980	1.3121845	5.6218786	6
CATGG	40640	1.311505	12.081629	5
TTTCA	59915	1.3105866	13.556667	5
CATCT	49885	1.3052672	15.087013	5
AGATT	65610	1.3048259	22.260387	4
TTTCC	44420	1.2980738	6.561359	5
TTTGC	43435	1.288856	8.878544	5
GATCG	39910	1.287947	19.296179	5
TCGCT	36250	1.286684	9.792917	7
GTCAC	40400	1.2839671	9.369286	6
TGTGG	34885	1.2767037	12.423427	5
AGTTG	47110	1.2709547	7.758247	5
TCTGA	47725	1.2679998	5.320583	7
TCTGG	35010	1.2618268	5.8370047	7
TGCAA	52730	1.2544103	6.528813	7
ATGGC	38600	1.2456716	6.3372617	6
TGAAC	52215	1.2421588	5.3637776	7
TGCTC	34895	1.2385886	5.3525434	7
GCCAT	38825	1.2339115	6.318348	8
CCCAG	32365	1.2304032	7.872932	1
GTCCA	38700	1.2299387	5.516783	6
CAGTA	51505	1.2252684	31.542398	4
TTCTC	41725	1.2193185	6.3478866	6
CAGTC	38360	1.2191331	52.727837	4
ATGAA	66670	1.1871939	9.221313	6
GTCGT	32875	1.1848773	7.51461	6
TCTCT	40170	1.1738772	5.696168	7
GATGC	36355	1.1732225	15.868876	5
TTTGT	47175	1.1702461	7.747171	5
GTGCC	27040	1.1657721	10.075911	6
GTGTG	31800	1.1638005	51.77286	4
TCGTC	32615	1.1576606	5.1460147	7
TCTCC	33095	1.1568646	6.2035055	7
ATCTC	44165	1.1556005	9.439147	6
GTCGA	35665	1.1509554	9.842315	6
TGTTA	51815	1.1508784	24.796684	4
GCCTT	31825	1.1296198	5.57765	3
ATCCA	47535	1.1136575	6.3614554	6
CTTTA	50170	1.0974234	24.780916	4
ATCGG	33980	1.0965784	6.5406804	6
TCGTT	36890	1.0946449	6.100514	7
GATTT	49180	1.0923516	6.1503806	5
GTGGA	33290	1.090872	7.753721	6
GTTCA	41045	1.0905197	15.345354	5
TCTAC	41355	1.0820754	5.1763725	7
TCGCC	25320	1.0750457	7.905519	7
GTGGC	24480	1.0716726	6.325536	6
ATGCT	40300	1.0707258	8.244864	6
GTGAA	44325	1.070716	10.30715	6
GTCTG	29430	1.060713	6.5709147	6
TCACT	40395	1.0569565	5.924917	7
GTCTC	29555	1.0490468	9.310896	6
ATCTG	39420	1.0473453	7.3559656	6
ATGGA	43245	1.0446275	7.671799	6
AGATA	58550	1.0426009	38.170498	4
GTCTT	34815	1.033073	8.63269	6
AAAGT	57705	1.0275539	8.169944	1
TCCGT	28915	1.0263301	5.9047346	3
CAGTT	38470	1.0221047	16.41561	4
CATCG	31995	1.0168446	14.949828	5
AGTTT	45710	1.0152785	8.003964	3
GTCAT	38205	1.015064	6.106353	6
TCTCA	38525	1.0080268	7.5235023	7
ATCTT	46010	1.0064274	8.994073	6
TTCAG	37875	1.0062963	5.771405	6
GTTAC	37715	1.0020453	5.1666555	5
GTGTA	36530	0.98552275	40.20628	4
TATTT	53810	0.9839972	5.1516094	7
TCACA	41795	0.9791798	8.020081	7
ATGGG	29845	0.97798365	5.036741	6
AGTCA	40875	0.972388	15.389681	5
ATGCC	30575	0.9717152	9.477156	6
CATCC	30985	0.96979576	9.425673	5
TAAGA	54185	0.9648732	6.0871816	7
GATCT	35950	0.9551512	18.978764	5
TCAGC	29995	0.9532821	8.306102	7
CATGA	40065	0.9531187	11.236544	5
TTCAC	36415	0.9528176	6.216642	6
TCAAT	48535	0.9505914	9.154862	7
TCGAA	39775	0.9462198	9.066514	7
ATATT	56310	0.92198753	6.1369042	6
TAGAA	51675	0.92017764	9.402753	7
ATCCC	29285	0.9165877	5.3419743	6
GTCAG	27995	0.90343463	8.887814	6
TATTC	41300	0.9034003	5.7062187	7
CCATA	38425	0.90022695	37.210354	4
GTAGA	36975	0.8931692	8.608813	6
GTTAT	39900	0.88623077	5.2348247	5
GTAAG	36685	0.886164	5.2355638	6
CATGC	27830	0.8844753	13.901799	5
ATCAT	45075	0.8828249	5.73596	6
TCTCG	24870	0.88275397	6.5400853	7
GTCCC	20705	0.8791003	5.537909	6
ATCGA	36795	0.87532777	8.512725	6
TTTCG	29235	0.86749643	10.288749	5
GTCGG	19795	0.866575	7.3444133	6
TTTAG	38900	0.86401945	7.561414	5
ATGAT	42915	0.8534767	5.1011553	6
ATGCA	35590	0.8466615	6.251892	6
GTTCG	23255	0.8381543	11.553167	5
TGTGC	23190	0.83581156	14.157436	5
GTGGG	18665	0.8297027	5.5177846	6
GTAAC	34105	0.8113344	5.8135676	6
ATCAC	34375	0.8053429	9.621701	6
TTTAA	48855	0.7999236	7.352625	5
AGTCT	30105	0.7998561	10.526578	5
ATCGT	30090	0.7994576	6.6603055	6
CATGT	29995	0.79693353	10.436387	5
GATAT	39940	0.79431105	8.622079	5
GATCC	24880	0.79072034	12.715061	5
TTCGA	29690	0.78883004	6.518596	6
GATAA	44270	0.78831667	14.775192	5
TCGAG	24415	0.7879034	5.195057	7
AGTAC	32870	0.7819546	5.2260313	5
TTCGC	21905	0.777512	6.6604743	6
GTTAA	38815	0.77193755	8.48706	5
AGTGG	23500	0.77006584	10.3609495	5
GTATT	34430	0.764735	5.8264165	6
TTAGA	38420	0.76408195	5.795453	6
ATCGC	24025	0.76354736	9.677485	6
TCGAT	28490	0.75694734	5.1531067	7
GTCTA	28445	0.7557518	6.2867093	6
AGTAT	37930	0.75433695	5.044013	5
AGTCC	23670	0.7522649	7.182083	5
TAAAA	56685	0.744089	8.344405	7
AAGTG	30775	0.7434018	5.0494637	2
TGTAG	27350	0.73786056	12.1410675	5
GTGAT	27035	0.72936237	5.6903186	6
AGTCG	22390	0.7225541	13.411861	5
TTAAA	48960	0.717777	5.5446362	6
ATCTA	36545	0.715759	5.811933	6
TGTGT	23525	0.70882326	9.263808	5
GATAC	28920	0.68798685	8.629296	5
AGTGC	21225	0.68495804	10.67315	5
CATAT	34740	0.68040675	6.6485786	5
ATAAA	51610	0.67747086	8.217001	6
AGTGA	27930	0.67467797	10.156326	5
TAGCC	21175	0.6729704	6.1501	1
CATAC	28715	0.67273945	6.6123075	5
TCGAC	20695	0.6577153	5.624726	7
TCGCA	20685	0.6573975	6.302775	7
GTAAA	36510	0.6501342	8.755067	6
GTTAG	23890	0.64451516	8.333901	5
TAGCA	26400	0.62803775	5.6752224	7
ATAAC	35715	0.6263226	5.033841	6
GTGCA	19250	0.62122226	6.775393	6
TCACG	18930	0.6016212	7.3660793	7
TAAAC	33435	0.586339	5.0168996	7
TGTGA	21700	0.58543235	12.232649	5
TGTAC	21680	0.5760133	6.08145	5
ATAAG	32190	0.57320786	5.2236843	6
GATAG	23185	0.5600576	14.022057	5
ATAGC	23290	0.554053	6.79403	6
AGTAA	30540	0.5438263	8.868569	5
CATAA	30865	0.54126966	10.392274	5
GTGAG	15950	0.52266175	5.4021826	6
ATAGA	29240	0.5206772	8.530579	6
TGTAA	25780	0.5127026	11.785439	5
GTAGC	15485	0.49972084	8.825224	6
AGTAG	20680	0.4995467	8.973179	5
TGTAT	22470	0.49908784	6.5273743	5
CATAG	18095	0.43046755	10.498208	5
>>END_MODULE