FastQCFastQC Report
Thu 31 Jul 2014
B80a_ACAGTG_L006_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename B80a_ACAGTG_L006_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 387699
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTCAGATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 994 0.25638446320470265 No Hit
TGACCATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 770 0.19860768276420623 No Hit
ACAGTGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 673 0.17358827337702704 No Hit
CGATGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 569 0.1467633396010823 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CAGTG 126530 3.933275 163.97636 2
GACCA 99545 3.5506406 222.79326 2
TCAGA 121580 3.285763 209.76965 2
CTCAG 102855 3.2297306 252.30225 1
TGACC 101910 3.200057 193.18152 1
ACCAT 116605 3.1832545 172.15 3
CAGAT 113385 3.064289 211.88159 3
TCTTC 142055 3.0055084 8.278248 7
TTCTT 185410 2.9722228 6.0802736 50-54
ACAGT 102450 2.7687647 148.75719 1
GCAGC 67060 2.7512975 7.956782 8
CACCA 71820 2.5876932 6.176166 40-44
AGCTT 104455 2.4851747 94.83831 2
ATCAG 91570 2.4747272 129.53354 2
GATCA 88835 2.4008121 129.3763 1
AGTGT 101830 2.3984096 129.44289 3
CGATG 74465 2.3147974 141.47276 1
TCTTG 108035 2.2627978 6.7633057 7
CAACA 72925 2.261398 5.1470556 35-39
TGTTG 107605 2.2311752 44.626194 4
TCAGT 92000 2.1888475 114.985954 3
CCATG 69685 2.1881657 95.534035 4
AGATG 81035 2.1680367 97.96242 4
GCGGC 44275 2.0893888 36.036674 8
GATGT 88520 2.0849183 98.80879 2
GGCAG 49615 2.0151477 11.224634 8
TGGCA 63850 1.9848226 15.945933 7
TCAGC 61960 1.9455944 6.1811666 7
CTTTG 90305 1.8914423 44.56064 4
TTTCT 117930 1.8904818 8.922644 5
AGATC 69230 1.8709769 70.41663 4
GCTTT 88920 1.8624334 87.08122 3
TTGGC 67520 1.8477654 22.781807 6
CCATC 57940 1.8378054 65.160286 4
GATGG 58555 1.8019582 34.899113 5
CCTTC 64505 1.8012251 5.618575 9
TAGCT 74940 1.7829589 94.66526 1
CATCG 55695 1.7488682 16.016222 5
CGGCA 42625 1.7487929 19.474224 9
TTTGG 82755 1.7159138 15.461836 5
GTTGG 62860 1.7029766 18.59949 5
CTGGA 53975 1.6778512 5.305263 8
AGCGG 40985 1.6646343 8.310168 8
AGTGG 54040 1.6630145 21.023024 5
ATGTT 91470 1.6488955 76.04886 3
TGTTC 78175 1.6373789 26.860275 4
GGAGG 40675 1.6354655 5.272287 75-79
TGCTG 59650 1.6323936 13.255112 7
CATCA 59490 1.6240454 5.0429263 5
ATGGC 51915 1.6138146 36.594254 6
GCCTT 58225 1.6095482 7.639639 8
ACACC 44645 1.6085708 5.5015755 40-44
TCTGG 57240 1.566441 15.07288 7
TTCTG 74475 1.5598822 6.935942 6
CATGG 49690 1.544649 26.827751 5
GTGTC 55895 1.5296334 52.502975 4
GGTCA 49090 1.5259975 5.755157 60-64
CAGTC 48435 1.5208985 51.44375 4
TCTCT 71730 1.5176172 12.176308 7
CTTTT 94545 1.5156077 6.062424 4
CTTTC 71160 1.5055575 28.260933 4
GTCTT 71140 1.4900305 6.0422926 6
GTGGA 48310 1.4866807 5.028218 6
GTTCT 70925 1.4855273 9.95201 5
TGGCC 40945 1.4788648 40.22463 7
GTGGC 40720 1.4559795 31.638351 6
TGGCG 40690 1.4549068 29.488676 7
GTGTG 52830 1.4312481 70.74636 4
GGCGG 30620 1.4304932 15.085451 8
GCTGG 39740 1.4209387 5.9289403 8
TTCTC 66395 1.4047426 10.258051 6
GTTGT 67195 1.3932792 10.49555 5
GTAGC 44240 1.3752319 7.671531 6
CGGCG 29130 1.3746786 12.7215185 8
TGGTC 50180 1.3732356 5.6788664 7
AGCCT 43020 1.3508629 6.6687813 2
GATGA 50180 1.342532 7.6792574 5
AAAGT 57565 1.3389544 11.3684 1
TGTCC 48290 1.3349091 23.401419 7
GGCCA 32390 1.3288774 8.294945 8
ATCTT 72060 1.3121662 7.2925286 6
GATGC 42185 1.3113507 45.18517 5
ATGGA 48240 1.2906286 5.59081 6
TTTCC 60915 1.2888002 10.278567 5
TTGCT 60870 1.2749249 6.6008234 6
TGTGG 46745 1.2663958 20.096905 5
CATCT 52480 1.2612512 18.433922 5
CAGTT 52825 1.2568029 8.97451 4
GCGGT 34840 1.2457349 6.7264 8
CCGGC 26130 1.2456045 16.502222 9
TTGGG 45945 1.2447225 9.654395 6
CAGTA 45865 1.2395256 15.252132 4
TTGCC 44725 1.2363597 22.744677 6
GTTGC 44850 1.2273738 25.793728 5
AGTAG 45730 1.2234753 5.35732 5
CTCTG 44155 1.220603 6.004489 8
TGCCC 33285 1.2143846 45.709206 7
TTTGC 56920 1.192192 19.934488 5
ATCGG 38300 1.1905828 16.277512 6
ATGGT 50215 1.1827176 8.290739 3
TGTCT 55650 1.1655917 13.7297125 5
CCCTT 41595 1.1614908 5.632114 8
TGGCT 42360 1.159232 12.512081 7
ATCTG 48505 1.1540223 11.869886 6
ATACC 42120 1.1498536 5.281175 6
GAGAT 42970 1.1496334 5.3148346 95-97
GCTGC 31600 1.1413391 5.1309605 8
TTGTC 54410 1.13962 10.32775 6
CCCAG 27485 1.1390685 8.01786 1
GATCT 47425 1.1283271 23.128399 5
TACCG 35785 1.123678 7.307782 7
TGCGG 31425 1.1236286 40.7918 7
GCCCC 23260 1.1200324 16.24926 8
TGCTT 53455 1.1196173 5.34159 7
GCCCA 26950 1.1168964 11.794938 8
AGTTT 61855 1.1150372 8.617882 3
CCATA 40840 1.1149104 18.517204 4
CCATT 46260 1.1117662 13.42344 4
ATGGG 35680 1.098008 13.697042 6
AAGTG 40885 1.0938506 5.526308 2
TGTGC 39910 1.0921848 33.30363 5
TCTGC 39455 1.090678 9.247984 7
TGCAG 34985 1.0875336 11.83134 7
GTTTG 52385 1.0861958 5.820808 4
GCCTC 29545 1.0779327 7.889437 8
TCCAG 34170 1.0729657 16.092346 9
CTGGC 29450 1.0636847 7.354961 8
ATTGC 44580 1.0606394 5.7446094 6
AGGGG 26335 1.0588809 5.322982 75-79
GCGGA 26015 1.0566174 8.704015 9
TTTGT 66550 1.0561271 8.4330015 5
GGGCA 25885 1.0513374 6.4197035 8
TGTCA 43940 1.0454127 6.4713373 7
TGTAG 44360 1.0448143 5.0931435 5
GATCG 33535 1.0424594 19.563158 5
GGGTC 29115 1.0410326 5.0924096 60-64
CCGGA 25340 1.0396343 5.4901795 9
TAGCC 32935 1.0341856 7.475734 1
TGTTT 64955 1.0308149 5.470679 4
ATGTC 43300 1.0301858 17.891344 6
AGATA 44220 1.0285515 21.776749 4
TGCCA 32740 1.0280626 9.439219 7
CTCCA 32270 1.0235758 16.270847 8
TCTCC 36605 1.0221509 25.88606 7
TTGTG 48755 1.0109283 7.027193 6
GGCGA 24805 1.0074724 6.0652413 8
AGTCC 32030 1.0057681 17.569128 5
CATGT 42145 1.0027063 16.818556 5
GTGCC 27745 1.0021029 36.967434 6
GGCCT 27440 0.99108684 11.277606 8
CATTG 41535 0.98819333 5.9983873 5
TGACG 31780 0.9879039 5.4710526 7
GTGTT 47530 0.9855281 8.585441 4
GGCCC 20545 0.97937024 21.67939 8
CCCGG 20495 0.9769869 11.810416 8
CATCC 30695 0.9736181 20.607687 5
TTAGC 40730 0.969041 5.617721 6
GTCTG 35290 0.965753 10.747388 6
TCGGC 26560 0.9593028 26.565418 7
CGGCC 20110 0.958634 10.446785 9
TGGGC 26775 0.9573637 25.414698 7
GTTCC 34565 0.9555008 12.491479 5
TGCCG 26425 0.9544267 29.314772 7
ATGAC 35270 0.95319015 5.1888695 6
GGCGT 26595 0.9509277 7.4545155 8
GATCC 30255 0.95003164 26.155773 5
ATGCC 30225 0.94908947 38.85304 6
CCGCC 19670 0.9471641 8.311403 9
GCCCT 25875 0.9440349 12.754001 8
AGATT 46090 0.94377315 13.412808 4
GATAG 35175 0.9410834 8.75591 5
CCGTC 25775 0.9403864 5.4129324 9
TCCGG 25905 0.93564516 21.907276 7
ATAGC 34445 0.9308941 8.700529 6
TGGGG 26155 0.92581046 5.440391 7
GATTG 39295 0.9255181 6.8860216 5
GTCCC 25350 0.92488056 24.092855 6
GTGCT 33795 0.9248405 10.389141 6
GCCTG 25580 0.92390674 7.792756 8
GTGTA 38825 0.91444814 13.57791 4
CGGCT 25310 0.91415477 9.894174 9
CGCCC 18955 0.9127349 5.399345 70-74
GCGCC 19105 0.91072613 12.133987 8
CCGGT 25155 0.90855646 5.743874 9
GTCTC 32665 0.902978 15.654558 6
GTCCT 32660 0.9028398 6.996301 8
TGCTC 32475 0.8977257 10.052156 7
CCTCG 24370 0.88912576 5.605987 85-89
TCCCC 24080 0.8874508 16.510614 7
CCGGG 18785 0.886486 6.6353245 8
TAGCG 28430 0.88376665 12.419138 7
CATGC 28120 0.8829909 41.791386 5
AGTCT 36885 0.87756133 13.034958 5
ATCTC 36090 0.8673506 19.529238 6
TTTCG 41310 0.8652398 7.8295918 5
TGTGT 41610 0.8627777 12.174437 5
TGCCT 31205 0.8626183 20.157925 7
ATCCT 35635 0.85641557 6.793285 6
GTGCG 23895 0.8543868 25.76142 6
AGTGC 27345 0.85003877 30.535408 5
GTCGG 23690 0.8470568 16.434607 6
GCCGG 17920 0.84566563 16.977278 8
CTTTA 46260 0.84236485 9.402595 4
TTGCG 30575 0.8367213 17.368307 6
CATGA 30935 0.83603454 6.276436 5
ATGCA 30855 0.8338724 7.6260653 6
TTCCC 29380 0.8204013 12.5910225 6
GTGGG 22980 0.81342477 12.3224 6
CCCGC 16720 0.8051136 11.159694 8
GATAC 29600 0.7999554 6.3157454 5
TTCGG 29170 0.79827183 9.606306 6
GCCGC 16585 0.7905989 11.209493 8
CGCTT 28415 0.7854927 5.0666137 2
TCCTG 28360 0.7839724 6.6880355 7
TGTTA 43310 0.7807333 9.133438 4
ATGCT 32655 0.77692187 12.019845 6
GCGTG 21725 0.7767965 5.478202 8
CGAGA 21910 0.773659 5.6105385 95-97
TGCGC 21225 0.76661146 20.909101 7
ATGTG 32470 0.7647683 10.471777 6
CATAG 28270 0.7640115 7.101938 5
TCCCG 20875 0.76161265 24.305128 7
TGTCG 27755 0.75954866 12.697839 5
CCCCG 15760 0.758887 8.708297 8
ATCCG 23935 0.7515784 13.123559 6
TCCCA 23630 0.7495226 6.6590524 7
CCCCT 20275 0.7472203 5.771568 9
ATCCC 23550 0.746985 22.576183 6
GGCGC 15770 0.74420464 7.047172 8
GCGCT 20300 0.733202 6.794583 8
CCCTC 19870 0.7322943 5.235509 9
TCCCT 25915 0.72364545 14.1209 7
GGGCC 15170 0.71588993 16.771355 8
GTTCG 25940 0.70987904 9.035766 5
CCGTG 19645 0.7095445 5.656315 4
TCGCC 19410 0.70816296 12.453282 7
CCCTG 19210 0.7008661 5.9789906 8
AGTCG 22120 0.68761593 13.820814 5
GCCGT 18775 0.6781215 6.09411 8
CCGCA 16360 0.678012 6.731353 9
TTCGC 24525 0.6779591 6.889077 6
CGGGC 14345 0.67695725 7.207335 8
CCCAC 15655 0.65537107 5.8659115 9
ATGCG 20970 0.6518673 26.466026 6
GCCCG 13670 0.6516424 13.590068 8
TGCGT 23755 0.6500839 9.141913 7
TCCGC 17670 0.64468 10.507457 7
TCCGT 23190 0.6410549 5.3746223 3
TGCAC 20275 0.6366515 5.039325 7
TTCCG 22925 0.63372934 8.323185 6
TGCGA 19920 0.61922735 5.049043 7
CTCCC 16770 0.6180461 7.0045033 8
GTGCA 19695 0.6122331 7.4605265 6
TCTCG 22135 0.611891 7.0097036 7
GGCCG 12020 0.5672378 6.887009 8
GTCCG 15660 0.565613 14.779968 6
CGCGG 11720 0.55308044 5.3769007 8
TCGCT 19830 0.5481725 7.1973443 7
ATCGC 16625 0.5220385 12.194863 6
CCCCC 10530 0.512188 5.5892406 9
CCCGT 13860 0.5056743 5.660583 9
TCGGG 13785 0.49289486 11.476487 7
GTCGC 13510 0.48795855 11.750425 6
TCGCG 13500 0.48759738 9.946709 7
GACTA 16120 0.43565142 5.202308 2
Produced by FastQC (version 0.10.1)