##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	B80a_ACAGTG_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	387699	
Filtered Sequences	0	
Sequence length	101	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.745119280679084	33.0	31.0	34.0	25.0	34.0
2	30.53791214318324	34.0	31.0	34.0	19.0	34.0
3	31.319074849303195	34.0	31.0	34.0	25.0	34.0
4	35.34474166814977	37.0	35.0	37.0	32.0	37.0
5	34.281615376877426	37.0	35.0	37.0	28.0	37.0
6	32.70839749393215	35.0	33.0	37.0	18.0	37.0
7	34.60077534375895	35.0	35.0	37.0	32.0	37.0
8	35.00806037673556	36.0	35.0	37.0	32.0	37.0
9	36.68023131346741	39.0	37.0	39.0	32.0	39.0
10-14	37.84768802602019	39.2	37.6	39.4	34.8	39.4
15-19	39.29315680463453	40.0	39.0	41.0	36.0	41.0
20-24	39.218460197214846	40.0	39.0	41.0	36.0	41.0
25-29	39.06180619501211	40.0	39.0	41.0	36.0	41.0
30-34	38.85567772937253	40.0	38.6	41.0	35.4	41.0
35-39	38.6494956138654	40.0	38.0	41.0	35.0	41.0
40-44	38.3687804198618	40.0	38.0	41.0	34.0	41.0
45-49	38.179245239219085	40.0	38.0	41.0	33.8	41.0
50-54	38.04174320800415	40.0	37.2	41.0	33.6	41.0
55-59	37.50208692826136	39.4	36.2	40.8	33.0	41.0
60-64	36.76902545531457	38.2	35.0	40.0	31.8	41.0
65-69	35.8671201112203	36.6	35.0	39.4	31.0	40.8
70-74	35.18217328391355	35.6	34.4	38.0	31.0	39.6
75-79	34.086485134085976	34.8	33.4	36.2	30.4	38.2
80-84	33.95390908926771	35.0	34.0	35.6	30.8	36.8
85-89	33.56909148592078	35.0	34.0	35.0	30.2	36.0
90-94	33.30880141553112	35.0	34.0	35.0	30.0	35.4
95-99	33.15386317736183	35.0	33.4	35.0	29.8	35.0
100-101	32.864174269214004	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	18.0
9	83.0
10	65.0
11	29.0
12	24.0
13	37.0
14	52.0
15	76.0
16	113.0
17	169.0
18	280.0
19	389.0
20	596.0
21	726.0
22	907.0
23	1215.0
24	1442.0
25	1782.0
26	2219.0
27	2725.0
28	3214.0
29	3980.0
30	4991.0
31	6480.0
32	8074.0
33	11604.0
34	17693.0
35	29881.0
36	59189.0
37	116543.0
38	103947.0
39	9151.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.136976937938007	17.98845994124217	30.988436726997907	35.88612639382192
2	30.883403710878554	28.14809464726534	22.71281576388226	18.25568587797385
3	13.080520311278828	48.67381497120129	15.095269170550774	23.150395546969108
4	17.853283088168915	23.38824706795736	13.728175723950798	45.03029411992293
5	35.67983327228926	14.350602788768576	30.302709841165033	19.666854097777136
6	31.685405430501497	40.115914665758744	27.342087547298288	0.8565923564414663
7	0.6528260325665013	0.6275486911237842	98.0714420207429	0.6481832555668186
8	47.36199990198582	11.670651716924727	8.71320276812682	32.25414561296263
9	29.22009084366996	9.663424460728555	26.203575454153867	34.91290924144762
10-14	26.851707123309577	18.133036195605353	25.97613096758052	29.03912571350455
15-19	21.661110750002322	25.55468889446028	32.739206200341094	20.044994155196306
20-24	21.242210391647063	27.03066320003302	31.36921711856712	20.357909289752797
25-29	21.81188627611229	28.087812821179586	29.770845458185256	20.32945544452287
30-34	22.192247826856338	27.163354263819045	29.926518878967933	20.717879030356684
35-39	21.9735782846527	27.011765920115593	29.657033749612964	21.357622045618744
40-44	21.360187707472335	27.19874875445648	29.589985907491013	21.851077630580175
45-49	21.584004623776327	26.973645507041454	29.547788482875	21.894561386307224
50-54	21.322688579543577	27.021822054889526	29.448524554972096	22.2069648105948
55-59	21.44900327742632	26.464120928826024	29.559175580980714	22.527700212766945
60-64	22.47902791719027	26.29361173972168	28.9953269219356	22.23203342115245
65-69	22.05413757972566	26.86518557666205	28.816793302167344	22.263883541444958
70-74	22.217303503194245	26.073665212720837	28.751185394087425	22.95784588999749
75-79	22.037442416819424	26.610665212458656	28.109259586964342	23.242632783757582
80-84	23.451859975990992	26.163320073523078	28.82293433014916	21.561885620336778
85-89	22.040655024560067	26.560058277601765	28.66426354105563	22.735023156782535
90-94	22.535081917140737	26.1724155366535	29.106924131839286	22.185578414366482
95-99	22.377347471731287	27.294798391631698	28.146445297627587	22.181408839009432
100-101	22.98530369567881	26.626814468024733	28.840868494193057	21.547013342103398
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	51.02310333175992
2	49.1390895888524
3	36.23091585824794
4	62.88357720809184
5	55.34668737006639
6	32.541997786942964
7	1.30100928813332
8	79.61614551494846
9	64.13300008511757
10-14	55.89083283681413
15-19	41.70610490519863
20-24	41.60011968139986
25-29	42.141341720635154
30-34	42.91012685721302
35-39	43.33120033027144
40-44	43.211265338052506
45-49	43.47856601008355
50-54	43.52965339013838
55-59	43.976703490193266
60-64	44.711061338342724
65-69	44.31802112117062
70-74	45.17514939319174
75-79	45.28007520057701
80-84	45.013745596327766
85-89	44.775678181342606
90-94	44.72066033150722
95-99	44.55875631074072
100-101	44.53231703778221
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	4.0
3	12.0
4	14.0
5	8.5
6	7.0
7	9.0
8	7.5
9	6.5
10	5.5
11	3.5
12	4.5
13	7.0
14	7.0
15	9.5
16	22.0
17	48.5
18	147.0
19	293.5
20	297.0
21	271.5
22	358.5
23	462.5
24	612.0
25	699.0
26	788.0
27	1074.0
28	1292.0
29	1442.5
30	1905.5
31	2263.0
32	2537.0
33	3124.5
34	3996.0
35	5099.0
36	6353.5
37	8470.5
38	10884.0
39	12533.0
40	14763.0
41	16806.0
42	18675.0
43	22048.0
44	25452.0
45	29059.0
46	30157.5
47	29042.5
48	26234.0
49	20590.5
50	16442.5
51	14277.5
52	12164.0
53	10264.5
54	8308.5
55	6216.5
56	4895.0
57	4513.5
58	3655.5
59	2322.0
60	1616.0
61	1308.5
62	1077.0
63	655.0
64	442.0
65	357.0
66	302.5
67	229.5
68	175.5
69	148.0
70	131.5
71	95.0
72	63.5
73	39.5
74	23.0
75	21.0
76	20.0
77	13.0
78	6.0
79	4.5
80	3.0
81	0.5
82	0.0
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0015475923332275811
2	0.007222097555062045
3	0.0020634564443034417
4	0.0
5	2.579320555379302E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0010833146332593067
20-24	7.737961666137906E-4
25-29	0.016868756432180634
30-34	0.00897603553271997
35-39	0.0358525557197723
40-44	0.03022963690904542
45-49	0.03533669160869644
50-54	0.03296371669774748
55-59	0.035800969308664714
60-64	0.04126912888606883
65-69	0.04709839334122605
70-74	0.016559237965535118
75-79	0.010936319154808239
80-84	0.0028888390220248184
85-89	0.009595072466011004
90-94	0.016352892321104776
95-99	0.006860992677308943
100-101	0.004255878916375849
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	387699.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	63.661926482425535
#Duplication Level	Relative count
1	100.0
2	25.937001594896334
3	10.333599149388624
4	4.470139996455786
5	2.136127355425601
6	1.2109398074310356
7	0.7273022623899816
8	0.5272018429913167
9	0.3588516746411483
10++	1.9744225884576763
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCAGATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA	994	0.25638446320470265	No Hit
TGACCATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA	770	0.19860768276420623	No Hit
ACAGTGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA	673	0.17358827337702704	No Hit
CGATGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA	569	0.1467633396010823	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CAGTG	126530	3.933275	163.97636	2
GACCA	99545	3.5506406	222.79326	2
TCAGA	121580	3.285763	209.76965	2
CTCAG	102855	3.2297306	252.30225	1
TGACC	101910	3.200057	193.18152	1
ACCAT	116605	3.1832545	172.15	3
CAGAT	113385	3.064289	211.88159	3
TCTTC	142055	3.0055084	8.278248	7
TTCTT	185410	2.9722228	6.0802736	50-54
ACAGT	102450	2.7687647	148.75719	1
GCAGC	67060	2.7512975	7.956782	8
CACCA	71820	2.5876932	6.176166	40-44
AGCTT	104455	2.4851747	94.83831	2
ATCAG	91570	2.4747272	129.53354	2
GATCA	88835	2.4008121	129.3763	1
AGTGT	101830	2.3984096	129.44289	3
CGATG	74465	2.3147974	141.47276	1
TCTTG	108035	2.2627978	6.7633057	7
CAACA	72925	2.261398	5.1470556	35-39
TGTTG	107605	2.2311752	44.626194	4
TCAGT	92000	2.1888475	114.985954	3
CCATG	69685	2.1881657	95.534035	4
AGATG	81035	2.1680367	97.96242	4
GCGGC	44275	2.0893888	36.036674	8
GATGT	88520	2.0849183	98.80879	2
GGCAG	49615	2.0151477	11.224634	8
TGGCA	63850	1.9848226	15.945933	7
TCAGC	61960	1.9455944	6.1811666	7
CTTTG	90305	1.8914423	44.56064	4
TTTCT	117930	1.8904818	8.922644	5
AGATC	69230	1.8709769	70.41663	4
GCTTT	88920	1.8624334	87.08122	3
TTGGC	67520	1.8477654	22.781807	6
CCATC	57940	1.8378054	65.160286	4
GATGG	58555	1.8019582	34.899113	5
CCTTC	64505	1.8012251	5.618575	9
TAGCT	74940	1.7829589	94.66526	1
CATCG	55695	1.7488682	16.016222	5
CGGCA	42625	1.7487929	19.474224	9
TTTGG	82755	1.7159138	15.461836	5
GTTGG	62860	1.7029766	18.59949	5
CTGGA	53975	1.6778512	5.305263	8
AGCGG	40985	1.6646343	8.310168	8
AGTGG	54040	1.6630145	21.023024	5
ATGTT	91470	1.6488955	76.04886	3
TGTTC	78175	1.6373789	26.860275	4
GGAGG	40675	1.6354655	5.272287	75-79
TGCTG	59650	1.6323936	13.255112	7
CATCA	59490	1.6240454	5.0429263	5
ATGGC	51915	1.6138146	36.594254	6
GCCTT	58225	1.6095482	7.639639	8
ACACC	44645	1.6085708	5.5015755	40-44
TCTGG	57240	1.566441	15.07288	7
TTCTG	74475	1.5598822	6.935942	6
CATGG	49690	1.544649	26.827751	5
GTGTC	55895	1.5296334	52.502975	4
GGTCA	49090	1.5259975	5.755157	60-64
CAGTC	48435	1.5208985	51.44375	4
TCTCT	71730	1.5176172	12.176308	7
CTTTT	94545	1.5156077	6.062424	4
CTTTC	71160	1.5055575	28.260933	4
GTCTT	71140	1.4900305	6.0422926	6
GTGGA	48310	1.4866807	5.028218	6
GTTCT	70925	1.4855273	9.95201	5
TGGCC	40945	1.4788648	40.22463	7
GTGGC	40720	1.4559795	31.638351	6
TGGCG	40690	1.4549068	29.488676	7
GTGTG	52830	1.4312481	70.74636	4
GGCGG	30620	1.4304932	15.085451	8
GCTGG	39740	1.4209387	5.9289403	8
TTCTC	66395	1.4047426	10.258051	6
GTTGT	67195	1.3932792	10.49555	5
GTAGC	44240	1.3752319	7.671531	6
CGGCG	29130	1.3746786	12.7215185	8
TGGTC	50180	1.3732356	5.6788664	7
AGCCT	43020	1.3508629	6.6687813	2
GATGA	50180	1.342532	7.6792574	5
AAAGT	57565	1.3389544	11.3684	1
TGTCC	48290	1.3349091	23.401419	7
GGCCA	32390	1.3288774	8.294945	8
ATCTT	72060	1.3121662	7.2925286	6
GATGC	42185	1.3113507	45.18517	5
ATGGA	48240	1.2906286	5.59081	6
TTTCC	60915	1.2888002	10.278567	5
TTGCT	60870	1.2749249	6.6008234	6
TGTGG	46745	1.2663958	20.096905	5
CATCT	52480	1.2612512	18.433922	5
CAGTT	52825	1.2568029	8.97451	4
GCGGT	34840	1.2457349	6.7264	8
CCGGC	26130	1.2456045	16.502222	9
TTGGG	45945	1.2447225	9.654395	6
CAGTA	45865	1.2395256	15.252132	4
TTGCC	44725	1.2363597	22.744677	6
GTTGC	44850	1.2273738	25.793728	5
AGTAG	45730	1.2234753	5.35732	5
CTCTG	44155	1.220603	6.004489	8
TGCCC	33285	1.2143846	45.709206	7
TTTGC	56920	1.192192	19.934488	5
ATCGG	38300	1.1905828	16.277512	6
ATGGT	50215	1.1827176	8.290739	3
TGTCT	55650	1.1655917	13.7297125	5
CCCTT	41595	1.1614908	5.632114	8
TGGCT	42360	1.159232	12.512081	7
ATCTG	48505	1.1540223	11.869886	6
ATACC	42120	1.1498536	5.281175	6
GAGAT	42970	1.1496334	5.3148346	95-97
GCTGC	31600	1.1413391	5.1309605	8
TTGTC	54410	1.13962	10.32775	6
CCCAG	27485	1.1390685	8.01786	1
GATCT	47425	1.1283271	23.128399	5
TACCG	35785	1.123678	7.307782	7
TGCGG	31425	1.1236286	40.7918	7
GCCCC	23260	1.1200324	16.24926	8
TGCTT	53455	1.1196173	5.34159	7
GCCCA	26950	1.1168964	11.794938	8
AGTTT	61855	1.1150372	8.617882	3
CCATA	40840	1.1149104	18.517204	4
CCATT	46260	1.1117662	13.42344	4
ATGGG	35680	1.098008	13.697042	6
AAGTG	40885	1.0938506	5.526308	2
TGTGC	39910	1.0921848	33.30363	5
TCTGC	39455	1.090678	9.247984	7
TGCAG	34985	1.0875336	11.83134	7
GTTTG	52385	1.0861958	5.820808	4
GCCTC	29545	1.0779327	7.889437	8
TCCAG	34170	1.0729657	16.092346	9
CTGGC	29450	1.0636847	7.354961	8
ATTGC	44580	1.0606394	5.7446094	6
AGGGG	26335	1.0588809	5.322982	75-79
GCGGA	26015	1.0566174	8.704015	9
TTTGT	66550	1.0561271	8.4330015	5
GGGCA	25885	1.0513374	6.4197035	8
TGTCA	43940	1.0454127	6.4713373	7
TGTAG	44360	1.0448143	5.0931435	5
GATCG	33535	1.0424594	19.563158	5
GGGTC	29115	1.0410326	5.0924096	60-64
CCGGA	25340	1.0396343	5.4901795	9
TAGCC	32935	1.0341856	7.475734	1
TGTTT	64955	1.0308149	5.470679	4
ATGTC	43300	1.0301858	17.891344	6
AGATA	44220	1.0285515	21.776749	4
TGCCA	32740	1.0280626	9.439219	7
CTCCA	32270	1.0235758	16.270847	8
TCTCC	36605	1.0221509	25.88606	7
TTGTG	48755	1.0109283	7.027193	6
GGCGA	24805	1.0074724	6.0652413	8
AGTCC	32030	1.0057681	17.569128	5
CATGT	42145	1.0027063	16.818556	5
GTGCC	27745	1.0021029	36.967434	6
GGCCT	27440	0.99108684	11.277606	8
CATTG	41535	0.98819333	5.9983873	5
TGACG	31780	0.9879039	5.4710526	7
GTGTT	47530	0.9855281	8.585441	4
GGCCC	20545	0.97937024	21.67939	8
CCCGG	20495	0.9769869	11.810416	8
CATCC	30695	0.9736181	20.607687	5
TTAGC	40730	0.969041	5.617721	6
GTCTG	35290	0.965753	10.747388	6
TCGGC	26560	0.9593028	26.565418	7
CGGCC	20110	0.958634	10.446785	9
TGGGC	26775	0.9573637	25.414698	7
GTTCC	34565	0.9555008	12.491479	5
TGCCG	26425	0.9544267	29.314772	7
ATGAC	35270	0.95319015	5.1888695	6
GGCGT	26595	0.9509277	7.4545155	8
GATCC	30255	0.95003164	26.155773	5
ATGCC	30225	0.94908947	38.85304	6
CCGCC	19670	0.9471641	8.311403	9
GCCCT	25875	0.9440349	12.754001	8
AGATT	46090	0.94377315	13.412808	4
GATAG	35175	0.9410834	8.75591	5
CCGTC	25775	0.9403864	5.4129324	9
TCCGG	25905	0.93564516	21.907276	7
ATAGC	34445	0.9308941	8.700529	6
TGGGG	26155	0.92581046	5.440391	7
GATTG	39295	0.9255181	6.8860216	5
GTCCC	25350	0.92488056	24.092855	6
GTGCT	33795	0.9248405	10.389141	6
GCCTG	25580	0.92390674	7.792756	8
GTGTA	38825	0.91444814	13.57791	4
CGGCT	25310	0.91415477	9.894174	9
CGCCC	18955	0.9127349	5.399345	70-74
GCGCC	19105	0.91072613	12.133987	8
CCGGT	25155	0.90855646	5.743874	9
GTCTC	32665	0.902978	15.654558	6
GTCCT	32660	0.9028398	6.996301	8
TGCTC	32475	0.8977257	10.052156	7
CCTCG	24370	0.88912576	5.605987	85-89
TCCCC	24080	0.8874508	16.510614	7
CCGGG	18785	0.886486	6.6353245	8
TAGCG	28430	0.88376665	12.419138	7
CATGC	28120	0.8829909	41.791386	5
AGTCT	36885	0.87756133	13.034958	5
ATCTC	36090	0.8673506	19.529238	6
TTTCG	41310	0.8652398	7.8295918	5
TGTGT	41610	0.8627777	12.174437	5
TGCCT	31205	0.8626183	20.157925	7
ATCCT	35635	0.85641557	6.793285	6
GTGCG	23895	0.8543868	25.76142	6
AGTGC	27345	0.85003877	30.535408	5
GTCGG	23690	0.8470568	16.434607	6
GCCGG	17920	0.84566563	16.977278	8
CTTTA	46260	0.84236485	9.402595	4
TTGCG	30575	0.8367213	17.368307	6
CATGA	30935	0.83603454	6.276436	5
ATGCA	30855	0.8338724	7.6260653	6
TTCCC	29380	0.8204013	12.5910225	6
GTGGG	22980	0.81342477	12.3224	6
CCCGC	16720	0.8051136	11.159694	8
GATAC	29600	0.7999554	6.3157454	5
TTCGG	29170	0.79827183	9.606306	6
GCCGC	16585	0.7905989	11.209493	8
CGCTT	28415	0.7854927	5.0666137	2
TCCTG	28360	0.7839724	6.6880355	7
TGTTA	43310	0.7807333	9.133438	4
ATGCT	32655	0.77692187	12.019845	6
GCGTG	21725	0.7767965	5.478202	8
CGAGA	21910	0.773659	5.6105385	95-97
TGCGC	21225	0.76661146	20.909101	7
ATGTG	32470	0.7647683	10.471777	6
CATAG	28270	0.7640115	7.101938	5
TCCCG	20875	0.76161265	24.305128	7
TGTCG	27755	0.75954866	12.697839	5
CCCCG	15760	0.758887	8.708297	8
ATCCG	23935	0.7515784	13.123559	6
TCCCA	23630	0.7495226	6.6590524	7
CCCCT	20275	0.7472203	5.771568	9
ATCCC	23550	0.746985	22.576183	6
GGCGC	15770	0.74420464	7.047172	8
GCGCT	20300	0.733202	6.794583	8
CCCTC	19870	0.7322943	5.235509	9
TCCCT	25915	0.72364545	14.1209	7
GGGCC	15170	0.71588993	16.771355	8
GTTCG	25940	0.70987904	9.035766	5
CCGTG	19645	0.7095445	5.656315	4
TCGCC	19410	0.70816296	12.453282	7
CCCTG	19210	0.7008661	5.9789906	8
AGTCG	22120	0.68761593	13.820814	5
GCCGT	18775	0.6781215	6.09411	8
CCGCA	16360	0.678012	6.731353	9
TTCGC	24525	0.6779591	6.889077	6
CGGGC	14345	0.67695725	7.207335	8
CCCAC	15655	0.65537107	5.8659115	9
ATGCG	20970	0.6518673	26.466026	6
GCCCG	13670	0.6516424	13.590068	8
TGCGT	23755	0.6500839	9.141913	7
TCCGC	17670	0.64468	10.507457	7
TCCGT	23190	0.6410549	5.3746223	3
TGCAC	20275	0.6366515	5.039325	7
TTCCG	22925	0.63372934	8.323185	6
TGCGA	19920	0.61922735	5.049043	7
CTCCC	16770	0.6180461	7.0045033	8
GTGCA	19695	0.6122331	7.4605265	6
TCTCG	22135	0.611891	7.0097036	7
GGCCG	12020	0.5672378	6.887009	8
GTCCG	15660	0.565613	14.779968	6
CGCGG	11720	0.55308044	5.3769007	8
TCGCT	19830	0.5481725	7.1973443	7
ATCGC	16625	0.5220385	12.194863	6
CCCCC	10530	0.512188	5.5892406	9
CCCGT	13860	0.5056743	5.660583	9
TCGGG	13785	0.49289486	11.476487	7
GTCGC	13510	0.48795855	11.750425	6
TCGCG	13500	0.48759738	9.946709	7
GACTA	16120	0.43565142	5.202308	2
>>END_MODULE