FastQCFastQC Report
Thu 31 Jul 2014
B18a_CTCAGA_L006_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename B18a_CTCAGA_L006_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 409234
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CAGTG 115570 3.515169 226.46571 2
GCTTT 109985 3.0828965 167.44026 3
TGACC 101475 3.0603805 158.30087 1
AAGAA 182110 2.7584388 5.361739 9
GACCA 99910 2.7034874 143.54178 2
ACAGT 118955 2.6841462 172.23936 1
TGGTG 78145 2.6717021 6.6808934 7
CGATG 87810 2.6708229 153.0849 1
AGTGT 104880 2.660124 195.92084 3
TGTTG 88495 2.5016637 33.645184 4
CTTTG 86855 2.4345589 45.704754 4
AGCTT 96120 2.4173427 146.03465 2
GATCA 101565 2.2917516 126.164406 1
TCAGA 101055 2.2802436 115.49525 2
TGCTG 67140 2.2760587 8.646304 7
CAAGA 111815 2.263719 5.497414 8
ACCAT 99410 2.2241743 120.35777 3
CTCAG 73540 2.2178903 154.61472 1
TGAAG 97255 2.2131972 7.900668 7
ATCAG 97100 2.191002 125.97991 2
GTGTC 64595 2.1897829 92.41743 4
CAGAT 96090 2.1682115 116.84139 3
GATGT 84625 2.1463864 115.78594 2
TTGGT 75340 2.129785 6.003663 6
TAGCT 84460 2.124103 146.28903 1
TTTGG 74560 2.1077354 12.269409 5
TCAGT 82865 2.0839899 140.56993 3
TCAAG 87160 1.9667116 14.05948 7
CCATC 64810 1.938089 56.432735 4
TTGAA 102830 1.9348682 7.6637774 6
AAAAA 171745 1.9299054 5.1001277 9
ATGTT 90480 1.8975149 95.74705 3
CTTTC 67350 1.8718816 58.259094 4
TCCAA 83170 1.860825 6.574369 7
GTTGC 54245 1.8389158 11.26116 5
GTGGT 53610 1.8328742 7.990352 6
TGCCA 60610 1.8279347 10.251256 7
TGGTT 64390 1.8202399 5.112834 7
TGGCC 43415 1.764956 5.440588 7
TTCTT 76065 1.7629247 7.619221 6
TTGCC 51560 1.7331268 7.464744 6
TGTCG 50910 1.7258587 21.272905 5
GTCAA 76150 1.7182779 22.779085 6
ATGGT 67000 1.6993545 13.027764 3
GTTGA 65950 1.6727229 8.831288 5
TTGCT 59630 1.671438 7.1081924 6
TGTTC 59430 1.665832 43.448063 4
GTTGG 48600 1.6615871 9.632819 5
AGATC 72515 1.6362562 40.99486 4
TGGTC 48025 1.6280566 6.1491046 4
GTGTT 57525 1.6261734 28.119919 4
TGTCT 57950 1.6243474 19.533167 5
TTTGA 77380 1.6227862 11.339603 5
TCAAA 96565 1.6164623 16.144451 7
TTCTG 57115 1.6009421 6.0208974 6
CCATG 52930 1.5963138 41.877037 4
CAAAA 106185 1.5948064 5.673228 8
GTTCT 56885 1.5944954 11.037531 5
TTCAA 84265 1.5721503 14.691458 6
TTGTT 67120 1.5688648 6.290115 6
TCTTC 55915 1.5540648 8.840713 7
TTTTG 65810 1.5382448 8.818681 5
CCATT 61000 1.5211414 18.346935 4
TGTCC 45235 1.5205197 13.252518 5
TTTCT 65420 1.5162102 13.9929285 5
TCAAC 67560 1.5115706 10.751154 7
TGCTA 60085 1.5110909 5.1461163 7
ATCAA 89455 1.4974437 13.11654 6
TGGCT 43815 1.4853369 7.3805904 7
GATGA 65145 1.4824815 9.027176 5
TGGAA 64455 1.4667796 7.8454976 7
CATTG 57675 1.450481 7.915158 5
GTCGC 35665 1.4498942 11.846787 6
TGTCA 57480 1.4455771 28.4653 5
GTGCT 42635 1.4453347 10.552835 6
AGATG 62775 1.4285482 29.72232 4
TCTTT 61515 1.4257058 8.271215 7
TTGGA 56000 1.420356 6.247481 6
AAGTT 74385 1.3996418 6.059884 2
TCCCA 46700 1.3965247 6.307593 7
TGAAA 82585 1.3942212 9.577651 7
CATCA 62080 1.3889624 17.338278 5
TTTCA 66130 1.3751382 18.706053 5
CTTTT 59285 1.3740221 18.468203 4
TTGTC 48860 1.3695533 5.3005643 6
GTCAC 44950 1.3556454 12.766232 6
GTGTG 39600 1.3538858 67.685875 4
GATGG 44065 1.3516978 10.234454 5
TGTTT 57740 1.3496165 13.728272 4
GTTCC 40110 1.348249 7.058229 5
GTTGT 47645 1.3468758 7.6358223 5
TTTCC 48295 1.3422796 8.868642 5
TCTTG 47360 1.3275081 5.5317497 7
GTTTG 46670 1.3193135 7.1148863 5
TGTGG 38400 1.3128589 16.380766 5
CATCT 52625 1.3122962 12.685444 5
GTTTC 46815 1.3122317 5.30186 4
CATGG 43075 1.3101662 9.54324 5
TTTGC 46290 1.2975157 11.540473 5
TCGGT 38060 1.2902412 7.00252 7
GATTG 50795 1.2883389 7.3307066 5
TCGCT 37700 1.2672397 8.899236 7
TCTGA 50080 1.2594728 5.0607543 7
TCTGG 37140 1.2590531 5.572428 7
CAGTA 55595 1.2544669 34.538116 4
AGTTG 49350 1.2516886 7.9702983 5
CAGTC 41495 1.251446 57.644913 4
TTGCA 49560 1.2463951 5.0240474 6
TGCAA 54550 1.2308872 6.9586225 7
TCTCT 44225 1.2291607 5.79498 7
TGCTC 36430 1.2245502 5.4438553 7
TTCTC 44040 1.2240188 7.317667 6
TGAAC 53555 1.2084355 5.25179 7
AGATT 64150 1.2070581 12.958599 4
GTCCA 39670 1.196406 7.4286885 6
GCCAT 39580 1.1936918 5.1622505 8
CTTTA 57220 1.1898595 33.634254 4
GATCG 39080 1.1886545 11.873735 5
GTCGT 34615 1.1734551 8.514593 6
TGTTA 55770 1.1695888 26.5264 4
ATGAA 69260 1.1692653 7.195342 6
TCTCC 35060 1.1685429 5.8988624 7
ATCTC 46860 1.1685361 6.093999 6
TTTGT 49745 1.1627411 9.895512 5
GTCGA 38185 1.1614324 11.827896 6
CGCTT 34335 1.1541293 6.9127045 2
GCCTT 34285 1.1524487 5.738849 3
GTGCC 28065 1.1409303 11.314569 6
TCACC 38015 1.1368068 5.0895743 7
TCGCC 27705 1.1167797 7.368742 7
GATGC 36615 1.1136793 9.51374 5
GTGGA 35910 1.1015425 9.191923 6
GTGGC 26840 1.1004272 8.568171 6
GTTCA 43565 1.0956258 15.708358 5
TCGTT 38800 1.08757 5.6676655 7
GTCTC 32135 1.0801791 11.017833 6
AGTTT 51410 1.0781523 11.229652 3
GTGTA 42475 1.0773147 51.935654 4
AAAGT 63270 1.0681405 10.849403 1
TCACT 42480 1.0593129 6.178788 7
GTGAA 46520 1.0586392 11.530723 6
TCCGT 31490 1.0584981 6.2442594 3
ATGCT 41970 1.0555127 5.828871 6
GTCTG 31035 1.0520924 8.28447 6
CAGTT 41585 1.0458301 18.417877 4
GTCAT 41260 1.0376568 6.7678313 6
ATGGA 45435 1.0339482 5.1198616 6
GTCTT 36760 1.0303885 10.628312 6
ATCGG 33850 1.0295794 5.161801 6
ATCTG 39950 1.0047113 5.1459904 6
CATCC 33535 1.0028362 7.569943 5
TCTCA 39955 0.9963478 6.6261764 7
AGTCA 44115 0.9954277 17.81428 5
CATCG 32635 0.98423773 12.373062 5
TCACA 43980 0.9839975 8.136595 7
TTTAC 47150 0.9804593 6.2219057 5
TAAGA 57870 0.9769762 6.131334 7
GTTAC 38740 0.9742808 5.5247564 5
TTCAG 38675 0.97264594 6.572722 6
TATTT 55790 0.96741384 5.2887034 7
TTTAT 55630 0.96463937 6.079735 5
CATGA 42315 0.9548119 9.837247 5
TCAAT 51090 0.9531972 9.725186 7
TCAGC 31555 0.95166606 8.569523 7
ATCTT 45550 0.94718814 6.856284 6
TCGAA 41615 0.9390169 8.785808 7
TTCAT 45035 0.9364789 5.011137 6
ATGCC 31050 0.9364359 6.653649 6
TTCAC 37400 0.93263423 6.8799305 6
GTCCC 23085 0.93054897 7.153564 6
GTCAG 30540 0.9289026 11.223229 6
TTTCG 33060 0.92667687 13.497868 5
GTAGA 40475 0.9210751 10.581782 6
TAGAA 54305 0.9167911 9.733187 7
TGGGA 29500 0.90491515 5.399271 7
TCTCG 26850 0.9025302 6.340298 7
GTCGG 21910 0.89829946 8.707329 6
GTAAG 38990 0.8872815 6.686715 6
GTTAT 42220 0.885423 5.2477446 5
TTTAG 42160 0.8841647 10.220898 5
AGATA 51945 0.8769489 23.52363 4
TTCGT 31145 0.87299913 5.3005643 6
TGTGC 25610 0.86818385 18.182253 5
CATGC 28710 0.8658638 10.852024 5
TATTC 41545 0.86390626 5.838067 7
TTCGG 25405 0.86123425 5.440792 6
GATCT 34175 0.8594745 11.561741 5
ATCGA 37960 0.8565441 6.5645776 6
GTGGG 20615 0.8524066 6.696409 6
GTCCG 20895 0.84944725 5.8938255 6
CCATA 37850 0.84684646 31.414165 4
GTTCG 24540 0.83191067 12.411935 5
TTTAA 52595 0.8182752 10.01944 5
GTAAC 36210 0.8170563 6.3676395 6
AGTAC 35485 0.8006971 5.372734 5
TTCGA 31745 0.79836184 7.304379 6
TAAAG 47055 0.7943946 5.3700337 7
TTCGC 23595 0.7931172 7.7907157 6
CATGT 31100 0.7821406 8.415191 5
ATCAC 34825 0.7791659 7.6156673 6
TGTAG 30690 0.77840585 14.919711 5
TCGAT 30940 0.7781168 5.804624 7
AGTCT 30800 0.77459586 11.208058 5
GTTAA 41065 0.7726866 8.823654 5
GATAT 40735 0.7664772 5.438363 5
TAAAA 60665 0.75978565 8.787475 7
AGTAT 40135 0.7551875 5.5113616 5
GTCTA 29865 0.7510814 7.1214657 6
GATCC 24830 0.748847 7.57594 5
AAGTG 32905 0.7488073 6.1148057 2
AGTCC 24800 0.7479422 8.117079 5
TTAGA 39630 0.7456854 7.2897124 6
TGTGT 26310 0.74375695 12.598421 5
AGCCT 24635 0.74296606 5.1636386 2
GTATT 35420 0.74281573 7.4434733 6
AGTGG 24140 0.74049664 10.799729 5
GTGAT 29160 0.7395997 6.579532 6
ATCGC 24365 0.73482317 6.2734404 6
TTAAA 52530 0.7332656 7.0052924 6
GATAA 43415 0.73294324 9.300349 5
GTGAC 23750 0.72237843 5.147053 6
AGTCG 23545 0.7161431 13.201234 5
AGTGC 23155 0.7042809 11.504988 5
TCAGG 23100 0.702608 5.029191 7
GTAAA 39830 0.6724203 10.952637 6
CATAT 36035 0.6723128 5.464799 5
TCGCA 22225 0.67028296 6.098108 7
TAGAG 29260 0.66585946 5.1530905 7
GTTAG 26130 0.6627483 9.397081 5
TCGAC 21940 0.6616877 5.9372473 7
CATAC 29360 0.6568933 5.2405434 5
ATAAA 52440 0.65677345 6.054525 6
AGTGA 28535 0.6493608 9.954172 5
TAGCA 28695 0.647485 5.8754406 7
TAGCC 21375 0.6446479 6.0266094 1
TTAGC 25535 0.64218533 6.158117 6
TGTGA 24965 0.6331998 15.915998 5
GTGCA 20430 0.6213975 8.435857 6
TGTAC 23920 0.6015693 8.866441 5
TCACG 19860 0.598957 7.8968306 7
TAAAC 35555 0.59517753 5.2110295 7
AGTAA 32375 0.5465631 10.04536 5
CATAA 32540 0.54470754 8.921419 5
GTGAG 17615 0.54034173 6.49979 6
TGTAA 28255 0.5316513 15.466487 5
ATAGA 30695 0.5182009 6.6796346 6
TGTAT 24555 0.51495886 8.2987585 5
GATAG 22450 0.5108866 8.960962 5
AGTAG 21700 0.49381915 9.876923 5
GTAGC 16175 0.4919777 10.043388 6
GTAAT 24905 0.46861702 5.3099027 6
CATAG 18820 0.42466167 8.939967 5
Produced by FastQC (version 0.10.1)