##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	B18a_CTCAGA_L006_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	409234	
Filtered Sequences	0	
Sequence length	101	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.03199392034875	31.0	30.0	34.0	16.0	34.0
2	29.73515152699922	33.0	30.0	34.0	16.0	34.0
3	30.395785785149815	34.0	31.0	34.0	22.0	34.0
4	33.75843893713621	37.0	35.0	37.0	26.0	37.0
5	33.19117912978882	37.0	35.0	37.0	19.0	37.0
6	32.66164101711979	37.0	33.0	37.0	17.0	37.0
7	34.008242228162864	36.0	35.0	37.0	28.0	37.0
8	34.739066157748375	37.0	35.0	37.0	32.0	37.0
9	36.753884085877516	39.0	37.0	39.0	32.0	39.0
10-14	37.1899446282567	39.2	37.2	39.4	33.0	39.4
15-19	38.29646021591559	40.0	38.0	41.0	33.0	41.0
20-24	38.236004828533304	40.0	38.0	41.0	33.0	41.0
25-29	38.08190570675946	40.0	38.0	41.0	32.6	41.0
30-34	37.852250301783336	40.0	38.0	41.0	32.0	41.0
35-39	37.62465728654023	40.0	37.8	41.0	31.4	41.0
40-44	37.50228280152675	40.0	37.6	41.0	31.0	41.0
45-49	37.30312583998397	40.0	37.0	41.0	30.8	41.0
50-54	36.65134128640338	39.2	36.0	40.2	30.4	40.6
55-59	37.14789191513902	39.8	36.0	41.0	31.0	41.0
60-64	36.540086112102124	38.8	35.0	40.6	30.8	41.0
65-69	35.653324503829104	36.8	35.0	39.6	30.0	41.0
70-74	34.628560676776615	35.6	34.0	38.0	29.0	39.6
75-79	33.72184911322128	35.0	33.8	36.4	28.8	38.2
80-84	33.00017593846063	35.0	33.0	35.4	27.8	36.6
85-89	32.556504591505096	35.0	33.0	35.0	27.0	35.8
90-94	32.2885190380076	35.0	33.0	35.0	26.4	35.0
95-99	32.03977773107807	35.0	32.0	35.0	25.2	35.0
100-101	31.40782046457528	34.5	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	11.0
7	233.0
8	807.0
9	754.0
10	558.0
11	494.0
12	552.0
13	708.0
14	845.0
15	923.0
16	981.0
17	1171.0
18	1131.0
19	1225.0
20	1362.0
21	1559.0
22	1799.0
23	2019.0
24	2275.0
25	2640.0
26	3191.0
27	4193.0
28	5164.0
29	5463.0
30	7436.0
31	8051.0
32	8534.0
33	11615.0
34	17635.0
35	29164.0
36	55019.0
37	114163.0
38	109078.0
39	8478.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.247882862736184	23.359508848595052	32.204437861868556	28.18817042680021
2	29.290860567194237	33.77332759817387	14.136353416167285	22.799458418464607
3	16.898781823159048	52.505035000586595	16.163818388017674	14.432364788236674
4	22.33394353701427	15.450699371628707	14.302718054126451	47.91263903723057
5	28.00466316684752	15.689063553978356	39.55235553470003	16.753917744474098
6	38.06594770810576	30.497088242266063	30.539365547173407	0.8975985024547719
7	0.700857499639552	1.1241089603702716	97.52353908600166	0.6514944539885097
8	25.7386110934621	26.882022609197143	13.609505051146833	33.76986124619392
9	26.24287655298476	34.16981906688953	22.409655630822165	17.17764874930354
10-14	19.327983280228704	33.71393687367452	26.41066960709417	20.547410239002602
15-19	21.05891698339179	31.276815720972294	25.415278575531303	22.248988720104613
20-24	20.66612377531728	31.04735667961655	26.416193673393174	21.870325871673
25-29	21.203586135979535	31.34877928861807	25.51529689135732	21.932337684045077
30-34	21.204592433501947	30.878000593385657	26.154034900265756	21.763372072846643
35-39	21.658371080531047	30.32377223044395	25.83335451216675	22.18450217685825
40-44	21.78446874809067	29.692381010570053	26.287334270177798	22.235815971161482
45-49	21.683753644475185	29.901730530009946	25.718202946862146	22.696312878652726
50-54	22.249943175846923	29.072703143292184	26.283553745872613	22.39379993498828
55-59	22.310402712450347	29.23016232271159	25.912142575713283	22.547292389124777
60-64	21.915275279580822	29.139604676624696	26.266813951669665	22.678306092124814
65-69	22.293651166064475	29.013087920166424	26.1274987095866	22.565762204182505
70-74	22.41296298559261	28.756125279532707	26.29384233744333	22.537069397431353
75-79	22.666581282984335	28.64961469798618	26.22502771710757	22.458776301921915
80-84	22.681217729169948	28.689641754066574	25.792612161452656	22.836528355310822
85-89	22.27447748404357	28.958018347511665	26.217818897633947	22.54968527081082
90-94	22.426151188821514	29.04197603534515	26.260310184318303	22.271562591515032
95-99	22.699762604080707	29.644082114740055	25.62243098114521	22.033724300034024
100-101	22.95166719220926	29.028650626617875	25.934426950801882	22.085255230370983
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	44.43605328953639
2	52.09031898565885
3	31.33114661139572
4	70.24658257424484
5	44.75858091132162
6	38.96354621056053
7	1.3523519536280617
8	59.508472339656024
9	43.4205253022883
10-14	39.87539351923131
15-19	43.3079057034964
20-24	42.53644964699028
25-29	43.135923820024615
30-34	42.96796450634859
35-39	43.8428732573893
40-44	44.02028471925215
45-49	44.38006652312791
50-54	44.6437431108352
55-59	44.857695101575125
60-64	44.59358137170564
65-69	44.859413370246976
70-74	44.95003238302397
75-79	45.12535758490625
80-84	45.51774608448077
85-89	44.824162754854385
90-94	44.69771378033654
95-99	44.733486904114734
100-101	45.03692242258025
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.5
2	3.5
3	14.0
4	20.0
5	16.0
6	14.5
7	12.5
8	14.0
9	11.0
10	7.5
11	11.5
12	15.5
13	20.5
14	19.5
15	23.5
16	31.0
17	38.5
18	39.5
19	41.5
20	68.5
21	121.5
22	185.0
23	232.5
24	326.0
25	428.5
26	564.5
27	784.0
28	1172.5
29	1643.0
30	2024.0
31	2679.0
32	3610.5
33	4875.0
34	6448.5
35	7820.0
36	9342.0
37	11667.0
38	14321.0
39	16892.5
40	19377.5
41	21938.5
42	24800.5
43	26875.5
44	27800.0
45	27773.5
46	27051.0
47	25741.0
48	23760.0
49	19922.0
50	14693.0
51	11232.0
52	9981.0
53	9036.0
54	7554.0
55	6229.0
56	4717.5
57	3570.5
58	2830.5
59	2143.5
60	1658.0
61	1153.0
62	820.5
63	563.0
64	468.5
65	397.5
66	315.0
67	267.5
68	239.0
69	190.5
70	126.5
71	108.0
72	88.0
73	59.0
74	50.0
75	36.0
76	18.5
77	17.0
78	17.5
79	13.0
80	8.0
81	4.5
82	3.5
83	4.0
84	4.0
85	3.5
86	3.0
87	2.5
88	3.0
89	2.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017838205036727155
2	0.015150256332562787
3	0.02394717936437344
4	0.019793076821573965
5	0.0171051281174096
6	0.007575128166281394
7	0.00513153843522288
8	0.004887179462117028
9	0.005375897408328731
10-14	0.01021420507582459
15-19	0.02419153833747929
20-24	0.016323179403470875
25-29	0.0033232820342395794
30-34	0.013830717877791191
35-39	0.004984923051359369
40-44	0.014417179413245234
45-49	0.012951025574610125
50-54	0.01881564092915056
55-59	0.02404492295361578
60-64	0.01221794865529257
65-69	0.015345743511047468
70-74	0.01686076914430375
75-79	0.024533640899827482
80-84	0.02893210241573281
85-89	0.021650205017178435
90-94	0.036213999814287176
95-99	0.03137569214679132
100-101	0.03249974342307824
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	409234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	59.33781931071434
#Duplication Level	Relative count
1	100.0
2	30.93051195597411
3	12.542727589815138
4	5.333488354067357
5	2.3718172305545866
6	1.1789326822462505
7	0.667364259969771
8	0.42863232957408054
9	0.27748711390148434
10++	1.289772507072821
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CAGTG	115570	3.515169	226.46571	2
GCTTT	109985	3.0828965	167.44026	3
TGACC	101475	3.0603805	158.30087	1
AAGAA	182110	2.7584388	5.361739	9
GACCA	99910	2.7034874	143.54178	2
ACAGT	118955	2.6841462	172.23936	1
TGGTG	78145	2.6717021	6.6808934	7
CGATG	87810	2.6708229	153.0849	1
AGTGT	104880	2.660124	195.92084	3
TGTTG	88495	2.5016637	33.645184	4
CTTTG	86855	2.4345589	45.704754	4
AGCTT	96120	2.4173427	146.03465	2
GATCA	101565	2.2917516	126.164406	1
TCAGA	101055	2.2802436	115.49525	2
TGCTG	67140	2.2760587	8.646304	7
CAAGA	111815	2.263719	5.497414	8
ACCAT	99410	2.2241743	120.35777	3
CTCAG	73540	2.2178903	154.61472	1
TGAAG	97255	2.2131972	7.900668	7
ATCAG	97100	2.191002	125.97991	2
GTGTC	64595	2.1897829	92.41743	4
CAGAT	96090	2.1682115	116.84139	3
GATGT	84625	2.1463864	115.78594	2
TTGGT	75340	2.129785	6.003663	6
TAGCT	84460	2.124103	146.28903	1
TTTGG	74560	2.1077354	12.269409	5
TCAGT	82865	2.0839899	140.56993	3
TCAAG	87160	1.9667116	14.05948	7
CCATC	64810	1.938089	56.432735	4
TTGAA	102830	1.9348682	7.6637774	6
AAAAA	171745	1.9299054	5.1001277	9
ATGTT	90480	1.8975149	95.74705	3
CTTTC	67350	1.8718816	58.259094	4
TCCAA	83170	1.860825	6.574369	7
GTTGC	54245	1.8389158	11.26116	5
GTGGT	53610	1.8328742	7.990352	6
TGCCA	60610	1.8279347	10.251256	7
TGGTT	64390	1.8202399	5.112834	7
TGGCC	43415	1.764956	5.440588	7
TTCTT	76065	1.7629247	7.619221	6
TTGCC	51560	1.7331268	7.464744	6
TGTCG	50910	1.7258587	21.272905	5
GTCAA	76150	1.7182779	22.779085	6
ATGGT	67000	1.6993545	13.027764	3
GTTGA	65950	1.6727229	8.831288	5
TTGCT	59630	1.671438	7.1081924	6
TGTTC	59430	1.665832	43.448063	4
GTTGG	48600	1.6615871	9.632819	5
AGATC	72515	1.6362562	40.99486	4
TGGTC	48025	1.6280566	6.1491046	4
GTGTT	57525	1.6261734	28.119919	4
TGTCT	57950	1.6243474	19.533167	5
TTTGA	77380	1.6227862	11.339603	5
TCAAA	96565	1.6164623	16.144451	7
TTCTG	57115	1.6009421	6.0208974	6
CCATG	52930	1.5963138	41.877037	4
CAAAA	106185	1.5948064	5.673228	8
GTTCT	56885	1.5944954	11.037531	5
TTCAA	84265	1.5721503	14.691458	6
TTGTT	67120	1.5688648	6.290115	6
TCTTC	55915	1.5540648	8.840713	7
TTTTG	65810	1.5382448	8.818681	5
CCATT	61000	1.5211414	18.346935	4
TGTCC	45235	1.5205197	13.252518	5
TTTCT	65420	1.5162102	13.9929285	5
TCAAC	67560	1.5115706	10.751154	7
TGCTA	60085	1.5110909	5.1461163	7
ATCAA	89455	1.4974437	13.11654	6
TGGCT	43815	1.4853369	7.3805904	7
GATGA	65145	1.4824815	9.027176	5
TGGAA	64455	1.4667796	7.8454976	7
CATTG	57675	1.450481	7.915158	5
GTCGC	35665	1.4498942	11.846787	6
TGTCA	57480	1.4455771	28.4653	5
GTGCT	42635	1.4453347	10.552835	6
AGATG	62775	1.4285482	29.72232	4
TCTTT	61515	1.4257058	8.271215	7
TTGGA	56000	1.420356	6.247481	6
AAGTT	74385	1.3996418	6.059884	2
TCCCA	46700	1.3965247	6.307593	7
TGAAA	82585	1.3942212	9.577651	7
CATCA	62080	1.3889624	17.338278	5
TTTCA	66130	1.3751382	18.706053	5
CTTTT	59285	1.3740221	18.468203	4
TTGTC	48860	1.3695533	5.3005643	6
GTCAC	44950	1.3556454	12.766232	6
GTGTG	39600	1.3538858	67.685875	4
GATGG	44065	1.3516978	10.234454	5
TGTTT	57740	1.3496165	13.728272	4
GTTCC	40110	1.348249	7.058229	5
GTTGT	47645	1.3468758	7.6358223	5
TTTCC	48295	1.3422796	8.868642	5
TCTTG	47360	1.3275081	5.5317497	7
GTTTG	46670	1.3193135	7.1148863	5
TGTGG	38400	1.3128589	16.380766	5
CATCT	52625	1.3122962	12.685444	5
GTTTC	46815	1.3122317	5.30186	4
CATGG	43075	1.3101662	9.54324	5
TTTGC	46290	1.2975157	11.540473	5
TCGGT	38060	1.2902412	7.00252	7
GATTG	50795	1.2883389	7.3307066	5
TCGCT	37700	1.2672397	8.899236	7
TCTGA	50080	1.2594728	5.0607543	7
TCTGG	37140	1.2590531	5.572428	7
CAGTA	55595	1.2544669	34.538116	4
AGTTG	49350	1.2516886	7.9702983	5
CAGTC	41495	1.251446	57.644913	4
TTGCA	49560	1.2463951	5.0240474	6
TGCAA	54550	1.2308872	6.9586225	7
TCTCT	44225	1.2291607	5.79498	7
TGCTC	36430	1.2245502	5.4438553	7
TTCTC	44040	1.2240188	7.317667	6
TGAAC	53555	1.2084355	5.25179	7
AGATT	64150	1.2070581	12.958599	4
GTCCA	39670	1.196406	7.4286885	6
GCCAT	39580	1.1936918	5.1622505	8
CTTTA	57220	1.1898595	33.634254	4
GATCG	39080	1.1886545	11.873735	5
GTCGT	34615	1.1734551	8.514593	6
TGTTA	55770	1.1695888	26.5264	4
ATGAA	69260	1.1692653	7.195342	6
TCTCC	35060	1.1685429	5.8988624	7
ATCTC	46860	1.1685361	6.093999	6
TTTGT	49745	1.1627411	9.895512	5
GTCGA	38185	1.1614324	11.827896	6
CGCTT	34335	1.1541293	6.9127045	2
GCCTT	34285	1.1524487	5.738849	3
GTGCC	28065	1.1409303	11.314569	6
TCACC	38015	1.1368068	5.0895743	7
TCGCC	27705	1.1167797	7.368742	7
GATGC	36615	1.1136793	9.51374	5
GTGGA	35910	1.1015425	9.191923	6
GTGGC	26840	1.1004272	8.568171	6
GTTCA	43565	1.0956258	15.708358	5
TCGTT	38800	1.08757	5.6676655	7
GTCTC	32135	1.0801791	11.017833	6
AGTTT	51410	1.0781523	11.229652	3
GTGTA	42475	1.0773147	51.935654	4
AAAGT	63270	1.0681405	10.849403	1
TCACT	42480	1.0593129	6.178788	7
GTGAA	46520	1.0586392	11.530723	6
TCCGT	31490	1.0584981	6.2442594	3
ATGCT	41970	1.0555127	5.828871	6
GTCTG	31035	1.0520924	8.28447	6
CAGTT	41585	1.0458301	18.417877	4
GTCAT	41260	1.0376568	6.7678313	6
ATGGA	45435	1.0339482	5.1198616	6
GTCTT	36760	1.0303885	10.628312	6
ATCGG	33850	1.0295794	5.161801	6
ATCTG	39950	1.0047113	5.1459904	6
CATCC	33535	1.0028362	7.569943	5
TCTCA	39955	0.9963478	6.6261764	7
AGTCA	44115	0.9954277	17.81428	5
CATCG	32635	0.98423773	12.373062	5
TCACA	43980	0.9839975	8.136595	7
TTTAC	47150	0.9804593	6.2219057	5
TAAGA	57870	0.9769762	6.131334	7
GTTAC	38740	0.9742808	5.5247564	5
TTCAG	38675	0.97264594	6.572722	6
TATTT	55790	0.96741384	5.2887034	7
TTTAT	55630	0.96463937	6.079735	5
CATGA	42315	0.9548119	9.837247	5
TCAAT	51090	0.9531972	9.725186	7
TCAGC	31555	0.95166606	8.569523	7
ATCTT	45550	0.94718814	6.856284	6
TCGAA	41615	0.9390169	8.785808	7
TTCAT	45035	0.9364789	5.011137	6
ATGCC	31050	0.9364359	6.653649	6
TTCAC	37400	0.93263423	6.8799305	6
GTCCC	23085	0.93054897	7.153564	6
GTCAG	30540	0.9289026	11.223229	6
TTTCG	33060	0.92667687	13.497868	5
GTAGA	40475	0.9210751	10.581782	6
TAGAA	54305	0.9167911	9.733187	7
TGGGA	29500	0.90491515	5.399271	7
TCTCG	26850	0.9025302	6.340298	7
GTCGG	21910	0.89829946	8.707329	6
GTAAG	38990	0.8872815	6.686715	6
GTTAT	42220	0.885423	5.2477446	5
TTTAG	42160	0.8841647	10.220898	5
AGATA	51945	0.8769489	23.52363	4
TTCGT	31145	0.87299913	5.3005643	6
TGTGC	25610	0.86818385	18.182253	5
CATGC	28710	0.8658638	10.852024	5
TATTC	41545	0.86390626	5.838067	7
TTCGG	25405	0.86123425	5.440792	6
GATCT	34175	0.8594745	11.561741	5
ATCGA	37960	0.8565441	6.5645776	6
GTGGG	20615	0.8524066	6.696409	6
GTCCG	20895	0.84944725	5.8938255	6
CCATA	37850	0.84684646	31.414165	4
GTTCG	24540	0.83191067	12.411935	5
TTTAA	52595	0.8182752	10.01944	5
GTAAC	36210	0.8170563	6.3676395	6
AGTAC	35485	0.8006971	5.372734	5
TTCGA	31745	0.79836184	7.304379	6
TAAAG	47055	0.7943946	5.3700337	7
TTCGC	23595	0.7931172	7.7907157	6
CATGT	31100	0.7821406	8.415191	5
ATCAC	34825	0.7791659	7.6156673	6
TGTAG	30690	0.77840585	14.919711	5
TCGAT	30940	0.7781168	5.804624	7
AGTCT	30800	0.77459586	11.208058	5
GTTAA	41065	0.7726866	8.823654	5
GATAT	40735	0.7664772	5.438363	5
TAAAA	60665	0.75978565	8.787475	7
AGTAT	40135	0.7551875	5.5113616	5
GTCTA	29865	0.7510814	7.1214657	6
GATCC	24830	0.748847	7.57594	5
AAGTG	32905	0.7488073	6.1148057	2
AGTCC	24800	0.7479422	8.117079	5
TTAGA	39630	0.7456854	7.2897124	6
TGTGT	26310	0.74375695	12.598421	5
AGCCT	24635	0.74296606	5.1636386	2
GTATT	35420	0.74281573	7.4434733	6
AGTGG	24140	0.74049664	10.799729	5
GTGAT	29160	0.7395997	6.579532	6
ATCGC	24365	0.73482317	6.2734404	6
TTAAA	52530	0.7332656	7.0052924	6
GATAA	43415	0.73294324	9.300349	5
GTGAC	23750	0.72237843	5.147053	6
AGTCG	23545	0.7161431	13.201234	5
AGTGC	23155	0.7042809	11.504988	5
TCAGG	23100	0.702608	5.029191	7
GTAAA	39830	0.6724203	10.952637	6
CATAT	36035	0.6723128	5.464799	5
TCGCA	22225	0.67028296	6.098108	7
TAGAG	29260	0.66585946	5.1530905	7
GTTAG	26130	0.6627483	9.397081	5
TCGAC	21940	0.6616877	5.9372473	7
CATAC	29360	0.6568933	5.2405434	5
ATAAA	52440	0.65677345	6.054525	6
AGTGA	28535	0.6493608	9.954172	5
TAGCA	28695	0.647485	5.8754406	7
TAGCC	21375	0.6446479	6.0266094	1
TTAGC	25535	0.64218533	6.158117	6
TGTGA	24965	0.6331998	15.915998	5
GTGCA	20430	0.6213975	8.435857	6
TGTAC	23920	0.6015693	8.866441	5
TCACG	19860	0.598957	7.8968306	7
TAAAC	35555	0.59517753	5.2110295	7
AGTAA	32375	0.5465631	10.04536	5
CATAA	32540	0.54470754	8.921419	5
GTGAG	17615	0.54034173	6.49979	6
TGTAA	28255	0.5316513	15.466487	5
ATAGA	30695	0.5182009	6.6796346	6
TGTAT	24555	0.51495886	8.2987585	5
GATAG	22450	0.5108866	8.960962	5
AGTAG	21700	0.49381915	9.876923	5
GTAGC	16175	0.4919777	10.043388	6
GTAAT	24905	0.46861702	5.3099027	6
CATAG	18820	0.42466167	8.939967	5
>>END_MODULE