FastQCFastQC Report
Thu 31 Jul 2014
B18a_CTCAGA_L006_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename B18a_CTCAGA_L006_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 409234
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
ACAGTGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 912 0.2228553834725365 No Hit
TGACCATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 776 0.1896225631301407 No Hit
CTCAGATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 705 0.17227307603962524 No Hit
CGATGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 627 0.15321307613736884 No Hit
TAGCTTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 542 0.13244256342337146 No Hit
GATCAGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 506 0.12364564039156081 No Hit
ACAGTGTGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAAT 441 0.10776230713968046 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CAGTG 155455 4.557986 218.70602 2
ACAGT 127560 3.2758222 199.28963 1
GACCA 93840 3.1856189 184.05539 2
TCTTC 151915 3.0464973 7.807507 7
TTCTT 194270 2.9471748 5.8454595 50-54
ACCAT 111725 2.9132605 142.78374 3
TGACC 96320 2.8675296 159.07292 1
GCAGC 73460 2.8472083 7.6482024 8
AGTGT 127900 2.8368661 171.81677 3
AGCTT 124490 2.8036635 126.71335 2
CACCA 76185 2.626022 6.269809 40-44
ATCAG 99670 2.5595894 139.13544 2
GATCA 97205 2.4962866 139.02336 1
TCAGA 96995 2.4908936 129.98343 2
CGATG 83175 2.4387152 152.05928 1
CAACA 75570 2.2469466 5.0680056 35-39
CTCAG 75300 2.2417457 155.70956 1
TCAGT 99315 2.2366924 122.841736 3
CAGAT 87095 2.2366555 131.25954 3
TGTTG 114100 2.2194207 47.77725 4
TCTTG 111155 2.1953614 6.061462 7
GCTTT 108555 2.1440103 115.95442 3
TAGCT 94300 2.1237488 126.59323 1
GATGT 93375 2.0710897 105.88913 2
GCGGC 46800 2.0709832 37.82507 8
GGCAG 53410 2.0387678 9.42019 8
CTTTG 101725 2.0091145 58.72891 4
CCATG 67370 2.005663 78.132454 4
TCAGC 65245 1.9424001 5.9035254 7
TTTCT 127550 1.9349984 12.658522 5
TGGCA 65315 1.9150549 14.1303005 7
TTGGC 72050 1.8526264 26.82828 6
CCTTC 67305 1.784216 5.385312 9
AGATG 70350 1.7792906 60.258728 4
CGGCA 45510 1.7639047 20.388943 9
CATCG 58320 1.7362366 13.293919 5
TTTGG 88635 1.724087 20.380306 5
CCATC 56820 1.7175751 53.37728 4
GTGTC 66310 1.7050335 67.25853 4
GTGTG 66615 1.6869524 93.73136 4
ATGTT 98295 1.6746457 81.69393 3
GTTGG 66110 1.6741637 18.527721 5
AGTGG 57210 1.6520269 22.96101 5
GGAGG 43835 1.6479462 5.476439 75-79
TGTTC 83130 1.6418552 27.865835 4
TGGTG 64365 1.6299735 5.5374436 4
TGCTG 63215 1.6254516 12.566408 7
AGCGG 42545 1.6240287 8.0136385 8
ACACC 46955 1.6184927 5.6511874 40-44
CTTTC 80410 1.6125389 39.281086 4
GATGG 55555 1.6042361 21.126928 5
TTCTG 79810 1.5762836 7.8425555 6
GCCTT 60300 1.5743247 7.1645703 8
TCTGG 60935 1.5668257 13.962673 7
AGATC 60475 1.5530369 43.03102 4
CAGTC 51790 1.5418329 53.262283 4
GGTCA 51780 1.5182047 5.7988434 60-64
CTTTT 99790 1.513865 7.6716084 4
GTGGA 52340 1.5113983 5.9221888 6
TCTCT 75245 1.50896 11.677229 7
GTTCT 74840 1.4781238 9.54716 5
ATGGC 50380 1.4771564 25.190033 6
GTCTT 74775 1.47684 6.454069 6
TGGCG 43740 1.4642308 29.70151 7
GTGGC 43690 1.4625571 37.508305 6
CATGG 49790 1.4598571 21.977573 5
TGGCC 42520 1.4452639 40.358902 7
GCTGG 43100 1.4428062 5.1774764 8
GGCGG 32945 1.4358106 15.2348995 8
TGTCC 54285 1.417284 24.519333 5
GTTTC 71510 1.412355 5.7168055 4
TTCTC 70365 1.4110968 12.737029 6
AATAA 72215 1.4011519 5.171669 20-24
CGGCG 31435 1.3910546 12.05768 8
GTAGC 46830 1.3730694 9.112196 6
TTTCC 68300 1.3696854 13.796995 5
AAAGT 61575 1.3640324 15.552925 1
GTTGT 69300 1.3479915 11.109672 5
GGCCA 34245 1.3272889 8.625368 8
AGCCT 44420 1.3224217 7.0153747 2
TGTGG 51855 1.3131714 26.471682 5
GCGGT 38535 1.2899892 6.703128 8
ACCGG 33265 1.2893054 5.130121 8
TTGCC 49350 1.2884398 25.911438 6
TTGCT 64490 1.2737066 8.005343 6
CCGGC 28225 1.2682002 18.320889 9
TTTGC 63610 1.2563262 26.38157 5
GTTGC 48735 1.2531264 26.728876 5
CAGTA 48380 1.2424294 15.6012335 4
CAGTT 55115 1.2412556 10.100321 4
TGCCC 35920 1.2396907 47.555172 7
TTGGG 48715 1.2336541 10.853747 6
CTCTG 47230 1.2330905 5.7341876 8
TTGCA 54685 1.2315714 5.3831315 6
CATCT 53445 1.2221417 14.379921 5
AGTAG 47845 1.2100945 5.640824 5
TGTGC 46220 1.188458 43.92063 5
TGTCT 60150 1.1879897 17.48557 5
CCCTT 44790 1.1873565 5.6680717 9
GATGC 40320 1.1821941 28.929085 5
AAGTT 59745 1.1606665 6.9139333 2
TGCGG 34485 1.1544124 43.205147 7
TTGTC 58295 1.1513525 12.429349 6
ATGGT 51880 1.1507164 9.474281 3
TGCAG 38765 1.1366011 12.196979 7
GTGAC 38700 1.1346953 5.728884 6
GCCCA 28615 1.1261213 12.783886 8
AGTTT 66010 1.1246079 11.613917 3
TGGCT 43720 1.1241753 12.354475 7
GCCCC 24585 1.1216236 15.749024 8
CCATT 48890 1.1179813 11.275543 4
TCTGC 42780 1.1169089 9.088623 7
CCATA 42755 1.1148485 16.194967 4
TACCG 37135 1.1055411 7.736649 7
ATCGG 37665 1.1043488 12.68031 6
TGCTT 55775 1.1015815 5.467765 7
GCTGC 32190 1.0941449 5.042803 8
GAGAT 43260 1.0941308 5.163106 95-97
GTTTG 56000 1.0892862 7.761681 4
AAGTG 42935 1.085911 6.6221614 2
GTGCC 31935 1.0854775 44.758995 6
CCCAG 27530 1.0834218 5.247117 9
TCCAG 36320 1.0812776 16.324905 9
ATCTG 47945 1.0797787 7.839937 6
TTTGT 71885 1.0740273 11.329632 5
TGCCA 35995 1.0716022 9.410998 7
GCCTC 31030 1.0709243 7.1115227 8
CCGGA 27600 1.0697379 5.731454 9
TGTCA 47290 1.0650274 6.092876 7
AGGGG 28260 1.062415 5.3415337 75-79
TGTAG 47740 1.0588896 6.409388 5
CTGGC 31040 1.0550562 6.229345 8
GGGTC 31315 1.0482942 5.125598 60-64
TAGCC 35080 1.044362 7.520598 1
GCGGA 27245 1.0399966 8.60587 9
GGGCA 27215 1.0388515 7.32887 8
ATGGG 35815 1.0342134 10.416332 6
GGCGA 27010 1.0310262 6.514551 8
AGTCC 34630 1.030965 18.41807 5
CCCGG 22855 1.0269163 12.090479 8
AGTGA 40520 1.0248308 5.138055 5
GATCT 45240 1.0188589 13.4306965 5
ATGTC 44995 1.0133413 12.535165 6
TGTTT 67520 1.0088103 6.135677 4
TGACG 34055 0.99850255 5.8426094 7
GTGTT 51315 0.9981556 12.5526085 4
TCTCC 37645 0.99794674 25.551308 7
TTGTG 51290 0.9976692 8.044511 6
GTCCC 28785 0.9934436 27.023787 6
TCGGC 29065 0.9879255 28.279247 7
CTCCA 32655 0.9871069 15.886949 8
CGCCA 25020 0.9846428 5.3043585 9
CCGCC 21515 0.98156327 8.692931 9
GGCCC 21730 0.9763681 21.806433 8
GTCTG 37770 0.9711826 11.893208 6
CGGCC 21575 0.9694037 10.042723 9
GCCCT 28060 0.96842206 14.741769 8
TCCGG 28480 0.9680412 22.560774 7
AGATA 43635 0.96661884 13.876656 4
CATTG 42915 0.966497 5.1429734 5
CATGT 42860 0.9652583 13.441614 5
GGCCT 28380 0.9646422 10.44816 8
TGGGC 28750 0.9624288 26.1633 7
GTGTA 43340 0.96129626 18.088242 4
GGCGT 28710 0.96108973 7.254959 8
CCGTC 27735 0.9572055 5.4884224 9
GTTCC 36550 0.9542549 13.088792 5
TTAGC 42300 0.95264655 5.7543826 6
CATCC 31470 0.9512863 17.587242 5
CCGGG 21460 0.9496432 7.165952 8
TGCCG 27910 0.9486668 30.059061 7
TCCCC 26880 0.94195324 17.092075 7
GTGCT 36405 0.9360843 12.005407 6
CGCCC 20480 0.93434423 5.4075313 70-74
GATCG 31845 0.9337047 12.1402645 5
ATAGC 36285 0.93182206 6.399789 6
GTCTC 35670 0.9312797 19.000034 6
GTGCG 27700 0.9272792 31.860144 6
CCGGT 27100 0.92113477 5.8832703 9
GATAG 36195 0.915443 5.2974696 5
GCCGG 20670 0.9146843 18.687258 8
CCTCG 26400 0.9111312 5.948694 85-89
CGGCT 26670 0.90651894 10.9755125 9
GCGCC 20110 0.9035786 12.918297 8
TGGGG 27400 0.9033545 5.2749496 7
GTCCT 34565 0.90243006 7.1898866 8
TGCTC 34365 0.8972085 9.26584 7
TTTCG 45240 0.89351034 10.504747 5
TGTGT 45865 0.89214474 16.145805 5
AGTGC 30360 0.890164 32.88107 5
AGATT 45810 0.8899511 8.514836 4
GTCGG 26570 0.88945156 19.070642 6
AGTCT 39330 0.8857586 13.3761 5
TGTGA 39655 0.87956166 6.1190305 5
TTGCG 34180 0.87887263 21.068676 6
GCCTG 25790 0.87660754 7.2181296 8
GTACC 29435 0.8763054 5.5282397 6
TAGCG 29845 0.87506413 11.898453 7
GATCC 29135 0.86737406 16.44058 5
ATCCT 37825 0.86495495 5.066726 6
GTGGG 26195 0.8636267 15.089552 6
TTCCC 32295 0.85612154 15.20483 6
CCCGC 18690 0.85268027 10.5067215 8
ATGCC 28560 0.8502559 28.622715 6
ATGCA 33030 0.84823155 5.839496 6
CTTTA 48935 0.8465139 11.82594 4
TTCGG 32695 0.84068877 11.930608 6
TGCCT 32160 0.83963984 18.632946 7
CATGC 27480 0.81810325 34.74317 5
CGCTT 31120 0.8124873 6.8358727 2
CGAGA 24135 0.8069191 5.814601 95-97
TGTTA 47200 0.8041433 9.879265 4
GCCGC 17855 0.80225736 10.391277 8
TGTCG 31160 0.8012192 17.254087 5
ATCTC 35025 0.80092657 13.903008 6
GCGTG 23885 0.799569 5.631925 9
CCCCT 22675 0.7945978 5.2329607 9
TCCTG 30415 0.79408103 6.6202655 7
CCCCG 17395 0.7935994 7.7860336 8
CATAG 30565 0.7849287 5.739958 5
TGCGC 22970 0.78075516 21.852146 7
TCCCG 22425 0.77394384 24.195911 7
TCCCA 25135 0.7597897 6.404609 7
ATGCT 33585 0.75637436 8.025562 6
GGCGC 16970 0.7509527 6.822673 8
ATCCG 25095 0.7470999 10.435816 6
CCCTG 21570 0.74443555 6.3920035 9
GTTCG 28530 0.7335938 9.62439 5
GCGCT 21580 0.73350877 6.8390956 8
TCGCC 21215 0.73218364 12.047756 7
CCCTC 20870 0.73134536 5.640724 9
TCCCT 27440 0.72741824 13.328324 7
TGTAC 32265 0.72664636 5.186651 5
ATGTG 32590 0.72285753 7.32339 6
GCCCG 15880 0.71351707 12.7440195 8
GGGCC 16090 0.7120111 16.498854 8
TTCGC 27140 0.70857674 7.7088747 6
CCCAC 17685 0.70667475 7.0326304 9
ATCCC 23045 0.6966125 16.224035 6
CCGTG 20485 0.6962895 6.2953405 4
AGTCG 23650 0.6934249 14.187335 5
CGGGC 15585 0.689664 7.7881455 8
GCCGT 20270 0.6889816 6.163426 8
CCGCA 17170 0.6757121 6.25838 9
TTCCG 25660 0.66993654 9.746853 6
TGCGT 25915 0.6663542 9.67414 7
TCCGC 19255 0.66453904 10.993577 7
GTGCA 22350 0.65530854 9.026902 6
TCCGT 24950 0.6513997 5.721599 3
CTCCC 18190 0.6374304 7.0678954 8
TGCAC 21200 0.63114226 5.0374823 7
TCTCG 23890 0.62372494 7.6708994 7
GGCCG 14080 0.62306505 7.9168754 8
ATGCG 21070 0.61777854 20.370947 6
GTCCG 17330 0.58905035 16.809343 6
TCGCT 21425 0.55936825 7.519001 7
CCCGT 15410 0.5318383 5.672485 8
CCCCC 11200 0.51882166 5.2554827 9
GTCGC 14975 0.5090034 14.106665 6
TCGCG 14665 0.49846646 9.311058 7
TCGGG 14690 0.49175924 11.150239 7
ATCGC 16155 0.48094833 8.602692 6
Produced by FastQC (version 0.10.1)