FastQCFastQC Report
Thu 31 Jul 2014
A80_CGATGT_L006_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename A80_CGATGT_L006_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 386824
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CGATG 113540 3.6637545 244.68279 1
TGACC 99075 3.1667325 172.81796 1
CTCAG 93705 2.9950912 229.77797 1
GATGT 108115 2.9008458 184.9883 2
TCAGA 118400 2.8259597 170.82158 2
GACCA 98270 2.8208113 156.60931 2
TGGTG 75560 2.7409036 6.895128 7
CAGAT 114120 2.7238054 172.6971 3
AAGAA 169940 2.720086 5.557253 9
TGTTG 89840 2.6841247 52.23887 4
GCTTT 89480 2.6480663 121.13875 3
CAGTG 81975 2.6452022 148.55205 2
ATGTT 112735 2.491315 152.09357 3
CTTTG 78125 2.312027 33.056152 4
ACCAT 97250 2.2991843 130.46715 3
CAAGA 106880 2.2909546 5.4048476 8
TGCTG 62490 2.2453406 8.572179 7
TGAAG 92385 2.2261062 8.704726 7
GATCA 90660 2.1638641 105.14275 1
TTGGT 71135 2.1252809 6.330786 6
TTTGG 70745 2.1136286 8.780779 5
ACAGT 86125 2.0556233 112.48261 1
AGCTT 75110 1.9962108 105.837845 2
TCAAG 83540 1.9939246 14.085079 7
ATCAG 82535 1.9699378 105.028366 2
CCATC 62215 1.9697554 60.962563 4
TTGAA 99020 1.9651628 8.61275 6
TTCCA 74360 1.9575727 5.4889617 6
AGTGT 72930 1.9567931 127.76347 3
TGTTC 65280 1.9318926 71.609695 4
GTTGC 53290 1.9147737 16.39792 5
TCCAA 80810 1.9105097 6.190594 7
AGATC 78930 1.8838941 60.254654 4
CAACA 88575 1.88062 5.394873 8
TGCCA 58765 1.8783047 10.601269 7
TCAGT 69455 1.8459167 117.44003 3
GTGTC 51140 1.8375216 57.701405 4
GTGGT 50650 1.837305 6.6838794 6
ATGGT 65350 1.7534133 19.300524 3
TTGCC 49175 1.7501931 7.36907 6
TTCTT 70325 1.7141302 7.5522804 6
TAGCT 64410 1.7118349 105.978264 1
GTTGA 63790 1.7115568 14.4570675 5
CTTTC 58290 1.7087039 41.017963 4
TTGGC 46810 1.6819396 5.5578346 6
TTGCT 56240 1.6643635 7.318402 6
GTTGG 45870 1.6639129 15.041646 5
ATTGG 61955 1.6623217 5.5293093 6
TGTCG 46040 1.6542726 13.801438 5
TGGTC 45665 1.6407982 8.800728 4
GTCAA 68675 1.6391282 17.46412 6
TCCAG 50955 1.6286738 5.3161335 7
CCATG 50925 1.6277149 45.996033 4
GTTCT 54930 1.6255955 17.151346 5
TCAAA 91895 1.6223443 16.23951 7
TTGTT 65210 1.6046425 6.43126 6
TTCAA 81160 1.5954659 15.813758 6
CAAAA 100495 1.5933139 5.773736 8
TCTTC 54285 1.5913019 9.679976 7
TTTGA 71940 1.5897919 7.7809725 5
TGGTA 58360 1.5658637 5.309509 4
AGATG 64900 1.5638286 42.6105 4
TTCTG 52835 1.5635961 5.6394744 6
TGTCT 52645 1.5579733 11.970061 5
ATCAA 88185 1.556847 16.812641 6
TGCCG 35965 1.5541412 5.133705 7
TTTTG 62865 1.5469384 6.014812 5
GATGA 63845 1.5384071 12.352272 5
CCATT 58340 1.5358361 19.931356 4
TGCTA 57775 1.5354955 5.0260596 7
TGCTT 51835 1.5340021 5.6109195 7
GTGTT 50455 1.50743 18.881344 4
TGGCT 41830 1.5030019 7.1957517 7
TCAAC 63520 1.5017396 12.346797 7
CATTG 55970 1.4875237 8.73908 5
GATGG 45475 1.4814271 15.390606 2
TGTCC 41560 1.4791667 7.9919 5
ATGTC 55520 1.475564 6.1728725 6
TTTCT 59705 1.4552739 9.37421 5
TGGAA 59895 1.443228 7.174097 7
TTGGA 53705 1.4409649 6.1277757 6
TCTTT 58430 1.4241966 8.155255 7
TGTTT 57845 1.4234097 21.876713 4
CATCA 60150 1.4220661 18.689566 5
GTGCT 39235 1.4097605 7.8924727 6
GTCGC 32485 1.4037613 9.575685 6
TGAAA 78535 1.3997308 9.688161 7
GTTGT 46840 1.3994257 11.780153 5
TTGTC 47085 1.3934308 5.4816265 6
TCCCA 43675 1.3827704 6.24836 7
GTTTT 55700 1.370627 5.2748947 5
GTTCC 38435 1.3679445 12.566097 5
TGTCA 51120 1.3586246 18.225754 5
GTTTG 45175 1.3496809 11.200564 5
CATGG 41580 1.34172 10.469621 5
CTTTT 54680 1.3327925 13.949914 4
TCTTG 44895 1.3286202 5.682671 7
TTTCC 45215 1.3254254 6.383319 5
TTTCA 60215 1.318088 13.227726 5
GTCAC 41015 1.3109617 8.291714 6
GATCG 40470 1.305902 17.684113 5
GATTG 48660 1.305602 11.8154955 5
TGTTA 58995 1.3037221 41.297554 4
ATTGA 65155 1.2930739 5.1868963 6
TTTGC 43635 1.2913316 8.539791 5
CATCT 48595 1.2792931 13.354807 5
AGATT 64320 1.2765024 20.339046 4
TCGGT 35335 1.2696288 6.2026334 7
TTGCA 47715 1.2681293 5.5156364 6
TCTGG 35035 1.2588495 5.0004377 7
CCCAG 32615 1.2537283 6.898765 1
TTCTC 42715 1.2521408 7.1069946 6
TGAAC 51950 1.2399377 5.254418 7
TCGCT 34825 1.2394605 9.388472 7
TGCAA 51925 1.2393409 6.7937074 7
ATGGC 38265 1.2347503 5.7266645 6
TCTCT 41930 1.2291293 5.6288843 7
AGTTG 45735 1.2271209 6.909723 5
TGTGG 33710 1.2228146 11.417577 5
GTCCA 37940 1.2126756 5.548474 6
GTTCA 45290 1.2036798 26.371918 5
GCCAT 37610 1.2021279 5.4401956 8
ATGAA 66955 1.1933403 8.51257 6
TCTCC 33645 1.1861292 5.6754985 7
CAGTA 49400 1.1790745 28.801838 4
GATGC 36475 1.1769899 13.7404 5
ATCCA 49025 1.159049 5.8465223 6
ATCTC 43815 1.1534568 7.7100763 6
CAGTC 36000 1.1506674 47.034702 4
GTGCC 26515 1.1457821 8.947083 6
GTCGT 31650 1.1372223 6.219896 6
TTTGT 46150 1.1356273 6.9576097 5
GTCGA 35020 1.1300393 8.496116 6
TTATT 61220 1.11428 5.771942 6
GATTT 49825 1.1010756 5.5624304 5
TCGTT 37085 1.0974914 6.156226 7
CTTTA 50130 1.0973305 25.013273 4
TCGCC 25480 1.0906357 7.8663626 7
ATCGG 33660 1.0861543 5.914424 6
GTGTG 29755 1.0793487 45.11026 4
ATGCT 40185 1.0680032 7.448685 6
GTCTC 30005 1.0679114 8.577114 6
TCACT 40220 1.0588161 6.1911974 7
CATCG 33010 1.055098 13.982386 5
GTGGA 32355 1.0540202 6.4606175 6
GTGAA 43635 1.0514276 9.382168 6
GTGGC 24060 1.0496299 6.7691436 6
GTCTT 35430 1.0485134 8.20809 6
TTCTA 47765 1.0455613 5.2327485 6
ATGGA 43375 1.0451627 6.823395 6
CAGTT 39175 1.0411602 15.777766 4
AGTTT 46880 1.0359945 7.181809 3
AAAGT 58095 1.0354283 7.0370283 1
GTTAC 38905 1.0339845 8.326617 5
GTCAT 38545 1.0244167 5.3223305 6
ATCTG 38445 1.021759 6.3146305 6
CATCC 32240 1.0207331 8.782955 5
TCACA 42940 1.0151874 8.093629 7
AGATA 56530 1.0075353 34.888958 4
TCTCA 37915 0.99813557 6.65075 7
GTCTG 27510 0.9884673 5.505566 6
ATCTT 44585 0.9759522 9.170578 6
TTCAC 36970 0.97325796 8.475978 6
TATTT 53405 0.97203726 5.657356 7
TTCAG 36530 0.9708638 7.0363026 6
TCAAT 49195 0.9670888 9.170117 7
AGTCA 40280 0.96139914 15.106095 5
TAAGA 53725 0.95754176 5.963275 7
ATGCC 29900 0.9556932 7.9662447 6
TCGAA 39955 0.9536421 8.911677 7
ATCGA 39755 0.94886863 7.846703 6
GATCT 35540 0.9445523 17.452381 5
CATGA 39545 0.94385624 10.464299 5
TCAGC 29370 0.93875283 8.493415 7
GTCCC 21930 0.9386829 5.1887593 6
TTCAT 42655 0.9337051 5.3282757 6
TAGAA 51845 0.9240344 9.8091545 7
GTTCG 25540 0.9176829 20.283932 5
ATATT 55970 0.91487557 5.7462955 6
TAACA 51675 0.9122875 5.0935745 7
GTTAT 41100 0.9082631 8.799145 5
GTGTA 33780 0.90635496 34.70701 4
ATCCC 28450 0.90074015 5.3117366 6
TGGGA 27440 0.8939055 5.3392305 7
TATTC 40185 0.8796375 6.2943206 7
GTAGA 36465 0.8786595 8.564295 6
GTCAG 27200 0.8777004 8.934221 6
TTTAG 39640 0.87599874 7.513033 5
CCATA 36870 0.8716805 33.861263 4
TTTCG 29420 0.87065387 9.558826 5
TCTCG 24435 0.86966884 6.1266685 7
TTCGT 29305 0.86725056 6.069968 6
TCGAG 26475 0.8543058 5.038343 7
ATGCA 35140 0.8387181 5.2890015 6
GTCGG 19220 0.8384824 6.007615 6
GTGGG 18845 0.8299784 5.5738387 6
CATGC 25795 0.8244851 11.734663 5
TTCGG 22930 0.8239025 5.7146387 6
TTCGA 30825 0.8192411 7.8224096 6
ATCAC 34500 0.8156491 9.102233 6
TTTAA 49300 0.80584896 7.3170476 5
TTCGC 22610 0.80471516 8.784207 6
TGTGC 22340 0.802703 11.762589 5
GTTAA 40440 0.8025771 13.446197 5
GATCC 24940 0.7971568 11.486638 5
GATAT 40145 0.79672235 8.0754175 5
TAAAG 44525 0.79357 5.315093 7
CATGT 29640 0.787747 9.705792 5
TCGAT 29520 0.78455776 5.245144 7
AGTCT 29500 0.78402627 9.241771 5
CGACG 20200 0.78391236 7.6046834 1
TTAGA 39270 0.7793571 8.016113 6
GTAAC 32505 0.7758263 5.323722 6
ATCGT 29030 0.771535 6.263081 6
GATAA 43270 0.771202 13.276999 5
ATCGC 23615 0.7548059 8.927155 6
TAAAA 56835 0.74926263 8.003446 7
AGTCC 23370 0.7469749 6.2006145 5
GTATT 33415 0.73843336 5.5292053 6
TTAAA 50255 0.7377203 7.103726 6
AGTGG 22560 0.7349312 9.116136 5
GTGAT 26850 0.7204154 5.0219135 6
ATCTA 36020 0.7080912 5.1854634 6
TGTAG 26295 0.70552415 9.993724 5
GTTAG 26020 0.69814557 14.613219 5
TCAGG 21365 0.6894143 5.1635194 7
CATAT 34875 0.68558234 6.2542706 5
TGTGT 22675 0.6774547 7.6940484 5
GATAC 28370 0.6771325 7.894527 5
AGTGC 20890 0.67408687 9.62454 5
TCGCA 21000 0.6712226 7.222502 7
ATAAA 50315 0.6633087 7.338433 6
GTAAA 37020 0.65980816 8.555781 6
TAGAG 27265 0.6569766 5.4214673 7
TCGAC 20530 0.65620005 5.1611443 7
AGTCG 20330 0.6560165 10.219226 5
TAGCC 20380 0.6514056 5.3161335 7
CATAC 27245 0.6441262 5.7329955 5
TTAGC 24155 0.6419714 6.4563875 6
AGTGA 26545 0.6396275 8.706189 5
TAGCA 26160 0.62438434 5.867819 7
GTGCA 19030 0.6140676 6.164769 6
TAAAC 34535 0.6096922 5.521605 7
TGTGA 22100 0.5929676 11.229927 5
TCACG 17980 0.57469445 7.5944767 7
TGTAC 21480 0.5708775 5.67138 5
GATAG 23255 0.5603517 13.263792 5
ATAGC 22545 0.53810203 6.53892 6
AGTAA 29860 0.5321953 8.298124 5
GTGAG 16080 0.52383393 5.22852 6
ATAGA 29325 0.5226601 7.389085 6
CATAA 29595 0.5224798 9.555265 5
AGTAG 20765 0.5003528 8.320546 5
TGTAT 22540 0.49810827 5.6803246 5
TGTAA 23960 0.475513 10.260304 5
GTAGC 14565 0.46998924 7.2600336 6
CATAG 17655 0.42138782 9.306744 5
Produced by FastQC (version 0.10.1)