##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	A80_CGATGT_L006_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	386824	
Filtered Sequences	0	
Sequence length	101	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.189874464872915	33.0	30.0	34.0	22.0	34.0
2	29.855427791450374	33.0	30.0	34.0	16.0	34.0
3	30.54198291729572	34.0	31.0	34.0	25.0	34.0
4	33.779106260211364	37.0	35.0	37.0	26.0	37.0
5	33.225174756478395	37.0	35.0	37.0	19.0	37.0
6	32.50422414327963	37.0	33.0	37.0	17.0	37.0
7	34.164576137985236	36.0	35.0	37.0	28.0	37.0
8	34.8725001551093	37.0	35.0	37.0	32.0	37.0
9	36.846963993961076	39.0	37.0	39.0	33.0	39.0
10-14	37.26246665150042	39.2	37.2	39.4	33.0	39.4
15-19	38.364356916840734	40.0	38.0	41.0	33.0	41.0
20-24	38.30247864661965	40.0	38.0	41.0	33.0	41.0
25-29	38.1624108121523	40.0	38.0	41.0	33.0	41.0
30-34	37.945396872996504	40.0	38.0	41.0	32.0	41.0
35-39	37.72566335077451	40.0	38.0	41.0	32.0	41.0
40-44	37.60428101668976	40.0	37.8	41.0	31.8	41.0
45-49	37.40093996236014	40.0	37.0	41.0	31.2	41.0
50-54	36.74987798068372	39.2	36.0	40.2	30.8	40.6
55-59	37.24044733522222	39.8	36.2	41.0	31.4	41.0
60-64	36.63609962153331	38.8	35.0	40.6	31.0	41.0
65-69	35.75499193431638	37.0	35.0	39.6	30.0	41.0
70-74	34.7301759973528	35.6	34.0	38.0	29.2	39.6
75-79	33.80705643910409	35.0	33.8	36.4	29.0	38.2
80-84	33.08661923768949	35.0	33.0	35.4	28.0	36.6
85-89	32.640158831923564	35.0	33.0	35.0	27.2	36.0
90-94	32.36852160155523	35.0	33.0	35.0	26.8	35.0
95-99	32.10681136640953	35.0	32.2	35.0	25.6	35.0
100-101	31.4593432672223	34.5	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	12.0
7	188.0
8	500.0
9	449.0
10	363.0
11	382.0
12	475.0
13	622.0
14	698.0
15	846.0
16	861.0
17	975.0
18	1030.0
19	1202.0
20	1316.0
21	1430.0
22	1653.0
23	1820.0
24	2143.0
25	2515.0
26	2947.0
27	4098.0
28	5021.0
29	5075.0
30	6981.0
31	7668.0
32	7912.0
33	11039.0
34	16662.0
35	27569.0
36	52347.0
37	108269.0
38	103694.0
39	8059.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.50690251665835	15.486511714497741	28.575565685739623	42.43102008310428
2	37.69246481659121	26.365773000757574	20.003154419160253	15.938607763490959
3	12.63928215869568	53.575372044011736	13.248774937615556	20.536570859677024
4	14.655355194365294	21.42439740811799	21.947996876502923	41.97225052101379
5	42.23386321091334	13.00716730962157	27.55690926579032	17.202060213674773
6	29.287865665274886	37.37638800915214	32.66226295582931	0.6734833697436626
7	0.3849425171466168	0.6972397372360145	98.54425029277864	0.37356745283872483
8	25.496243594980427	26.944773348913927	13.545291438085242	34.0136916180204
9	26.209360522832558	34.21497559563203	22.492347782925215	17.08331609861019
10-14	19.23958552555717	33.753792170199475	26.504109013872867	20.502513290370487
15-19	21.09471441389493	31.237578575288765	25.457591969611954	22.21011504120435
20-24	20.61728471670638	31.06228901906275	26.51983841342335	21.800587850807517
25-29	21.12941646075087	31.376344786543008	25.532731175056604	21.961507577649513
30-34	21.140550552838448	30.79001446863698	26.29935909833192	21.770075880192653
35-39	21.50939493903889	30.406510790994922	25.897353698513975	22.18674057145221
40-44	21.751089020440077	29.639247758872468	26.343332050916104	22.26633116977136
45-49	21.65271187632636	29.867819040015224	25.817637641530116	22.6618314421283
50-54	22.191912443063583	29.035762896279532	26.29573013069002	22.47659452996686
55-59	22.348838628467643	29.216481474011484	25.895063356470775	22.53961654105009
60-64	21.900484777971688	29.15664145821214	26.307026048736343	22.635847715079827
65-69	22.122861867790803	29.168058561991998	26.144293091269375	22.56478647894782
70-74	22.376488705462943	28.800487741563163	26.333390388202364	22.48963316477153
75-79	22.683426836130145	28.80930807930361	26.253237430907227	22.25402765365902
80-84	22.56105381838493	28.883033193003506	25.77474476866266	22.781168219948903
85-89	22.280935832195606	28.96632327996359	26.295302221670596	22.457438666170205
90-94	22.42420637486261	29.205922286157627	26.232624296890155	22.137247042089612
95-99	22.79471470079865	29.606095430392536	25.69037514080581	21.908814728003005
100-101	23.0089605503045	29.036449915307934	25.8697423033657	22.084847231021865
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	55.937922599762636
2	53.631072580082176
3	33.175853018372706
4	56.62760571537908
5	59.43592342458811
6	29.961349035018547
7	0.7585099699853417
8	59.50993521300083
9	43.29267662144275
10-14	39.742098815927655
15-19	43.30482945509928
20-24	42.417872567513896
25-29	43.09092403840039
30-34	42.910626433031105
35-39	43.696135510491104
40-44	44.01742019021143
45-49	44.31454331845466
50-54	44.668506973030446
55-59	44.888455169517734
60-64	44.53633249305152
65-69	44.68764834673863
70-74	44.86612187023448
75-79	44.93745448978916
80-84	45.34222203833384
85-89	44.738374498365815
90-94	44.561453416952226
95-99	44.70352942880165
100-101	45.09380778132637
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.5
3	7.0
4	10.5
5	6.5
6	8.0
7	16.5
8	16.5
9	13.0
10	12.0
11	18.5
12	19.5
13	13.0
14	16.0
15	22.0
16	27.0
17	31.0
18	44.5
19	64.5
20	79.0
21	106.0
22	168.5
23	230.5
24	300.0
25	400.5
26	531.5
27	753.0
28	1117.0
29	1610.5
30	2023.0
31	2638.5
32	3530.0
33	4721.0
34	6002.0
35	7286.5
36	8885.5
37	11016.0
38	13569.0
39	15946.5
40	18329.0
41	20832.5
42	23360.5
43	25325.5
44	26140.5
45	26032.5
46	25435.5
47	23963.5
48	22311.0
49	19227.0
50	14121.0
51	10571.0
52	9394.0
53	8509.0
54	7222.0
55	5940.5
56	4421.5
57	3393.5
58	2738.5
59	2060.5
60	1545.5
61	1064.5
62	756.5
63	589.0
64	464.0
65	342.0
66	293.5
67	257.5
68	215.5
69	172.0
70	130.0
71	96.0
72	71.5
73	55.0
74	47.5
75	42.5
76	23.5
77	18.0
78	17.0
79	9.5
80	7.0
81	4.0
82	1.5
83	2.0
84	3.0
85	2.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.020939755547742644
2	0.016803507538311998
3	0.028178189564246274
4	0.020164209045974397
5	0.017579054040080245
6	0.007496949517093045
7	0.0033607015076623998
8	0.004653279010609477
9	0.0020681240047153225
10-14	0.010392323123694498
15-19	0.024041941554815625
20-24	0.015304117634893389
25-29	0.003981138709076996
30-34	0.014580274233243025
35-39	0.005170310011788307
40-44	0.014063243232064196
45-49	0.013235993630178066
50-54	0.0198539904452671
55-59	0.023680019853990444
60-64	0.01266725952888135
65-69	0.01582114863607222
70-74	0.015976257936425868
75-79	0.02523111285752694
80-84	0.02998779806837218
85-89	0.022749364051868552
90-94	0.03851880958782289
95-99	0.03133207867143714
100-101	0.03334849957603458
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	386824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	58.16774554032046
#Duplication Level	Relative count
1	100.0
2	29.873944159007944
3	11.670600377939849
4	4.741703157846805
5	2.0505892977756193
6	1.0480621096329126
7	0.5987842175962115
8	0.35517238762360837
9	0.26562037535953614
10++	1.1785955512381705
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CGATG	113540	3.6637545	244.68279	1
TGACC	99075	3.1667325	172.81796	1
CTCAG	93705	2.9950912	229.77797	1
GATGT	108115	2.9008458	184.9883	2
TCAGA	118400	2.8259597	170.82158	2
GACCA	98270	2.8208113	156.60931	2
TGGTG	75560	2.7409036	6.895128	7
CAGAT	114120	2.7238054	172.6971	3
AAGAA	169940	2.720086	5.557253	9
TGTTG	89840	2.6841247	52.23887	4
GCTTT	89480	2.6480663	121.13875	3
CAGTG	81975	2.6452022	148.55205	2
ATGTT	112735	2.491315	152.09357	3
CTTTG	78125	2.312027	33.056152	4
ACCAT	97250	2.2991843	130.46715	3
CAAGA	106880	2.2909546	5.4048476	8
TGCTG	62490	2.2453406	8.572179	7
TGAAG	92385	2.2261062	8.704726	7
GATCA	90660	2.1638641	105.14275	1
TTGGT	71135	2.1252809	6.330786	6
TTTGG	70745	2.1136286	8.780779	5
ACAGT	86125	2.0556233	112.48261	1
AGCTT	75110	1.9962108	105.837845	2
TCAAG	83540	1.9939246	14.085079	7
ATCAG	82535	1.9699378	105.028366	2
CCATC	62215	1.9697554	60.962563	4
TTGAA	99020	1.9651628	8.61275	6
TTCCA	74360	1.9575727	5.4889617	6
AGTGT	72930	1.9567931	127.76347	3
TGTTC	65280	1.9318926	71.609695	4
GTTGC	53290	1.9147737	16.39792	5
TCCAA	80810	1.9105097	6.190594	7
AGATC	78930	1.8838941	60.254654	4
CAACA	88575	1.88062	5.394873	8
TGCCA	58765	1.8783047	10.601269	7
TCAGT	69455	1.8459167	117.44003	3
GTGTC	51140	1.8375216	57.701405	4
GTGGT	50650	1.837305	6.6838794	6
ATGGT	65350	1.7534133	19.300524	3
TTGCC	49175	1.7501931	7.36907	6
TTCTT	70325	1.7141302	7.5522804	6
TAGCT	64410	1.7118349	105.978264	1
GTTGA	63790	1.7115568	14.4570675	5
CTTTC	58290	1.7087039	41.017963	4
TTGGC	46810	1.6819396	5.5578346	6
TTGCT	56240	1.6643635	7.318402	6
GTTGG	45870	1.6639129	15.041646	5
ATTGG	61955	1.6623217	5.5293093	6
TGTCG	46040	1.6542726	13.801438	5
TGGTC	45665	1.6407982	8.800728	4
GTCAA	68675	1.6391282	17.46412	6
TCCAG	50955	1.6286738	5.3161335	7
CCATG	50925	1.6277149	45.996033	4
GTTCT	54930	1.6255955	17.151346	5
TCAAA	91895	1.6223443	16.23951	7
TTGTT	65210	1.6046425	6.43126	6
TTCAA	81160	1.5954659	15.813758	6
CAAAA	100495	1.5933139	5.773736	8
TCTTC	54285	1.5913019	9.679976	7
TTTGA	71940	1.5897919	7.7809725	5
TGGTA	58360	1.5658637	5.309509	4
AGATG	64900	1.5638286	42.6105	4
TTCTG	52835	1.5635961	5.6394744	6
TGTCT	52645	1.5579733	11.970061	5
ATCAA	88185	1.556847	16.812641	6
TGCCG	35965	1.5541412	5.133705	7
TTTTG	62865	1.5469384	6.014812	5
GATGA	63845	1.5384071	12.352272	5
CCATT	58340	1.5358361	19.931356	4
TGCTA	57775	1.5354955	5.0260596	7
TGCTT	51835	1.5340021	5.6109195	7
GTGTT	50455	1.50743	18.881344	4
TGGCT	41830	1.5030019	7.1957517	7
TCAAC	63520	1.5017396	12.346797	7
CATTG	55970	1.4875237	8.73908	5
GATGG	45475	1.4814271	15.390606	2
TGTCC	41560	1.4791667	7.9919	5
ATGTC	55520	1.475564	6.1728725	6
TTTCT	59705	1.4552739	9.37421	5
TGGAA	59895	1.443228	7.174097	7
TTGGA	53705	1.4409649	6.1277757	6
TCTTT	58430	1.4241966	8.155255	7
TGTTT	57845	1.4234097	21.876713	4
CATCA	60150	1.4220661	18.689566	5
GTGCT	39235	1.4097605	7.8924727	6
GTCGC	32485	1.4037613	9.575685	6
TGAAA	78535	1.3997308	9.688161	7
GTTGT	46840	1.3994257	11.780153	5
TTGTC	47085	1.3934308	5.4816265	6
TCCCA	43675	1.3827704	6.24836	7
GTTTT	55700	1.370627	5.2748947	5
GTTCC	38435	1.3679445	12.566097	5
TGTCA	51120	1.3586246	18.225754	5
GTTTG	45175	1.3496809	11.200564	5
CATGG	41580	1.34172	10.469621	5
CTTTT	54680	1.3327925	13.949914	4
TCTTG	44895	1.3286202	5.682671	7
TTTCC	45215	1.3254254	6.383319	5
TTTCA	60215	1.318088	13.227726	5
GTCAC	41015	1.3109617	8.291714	6
GATCG	40470	1.305902	17.684113	5
GATTG	48660	1.305602	11.8154955	5
TGTTA	58995	1.3037221	41.297554	4
ATTGA	65155	1.2930739	5.1868963	6
TTTGC	43635	1.2913316	8.539791	5
CATCT	48595	1.2792931	13.354807	5
AGATT	64320	1.2765024	20.339046	4
TCGGT	35335	1.2696288	6.2026334	7
TTGCA	47715	1.2681293	5.5156364	6
TCTGG	35035	1.2588495	5.0004377	7
CCCAG	32615	1.2537283	6.898765	1
TTCTC	42715	1.2521408	7.1069946	6
TGAAC	51950	1.2399377	5.254418	7
TCGCT	34825	1.2394605	9.388472	7
TGCAA	51925	1.2393409	6.7937074	7
ATGGC	38265	1.2347503	5.7266645	6
TCTCT	41930	1.2291293	5.6288843	7
AGTTG	45735	1.2271209	6.909723	5
TGTGG	33710	1.2228146	11.417577	5
GTCCA	37940	1.2126756	5.548474	6
GTTCA	45290	1.2036798	26.371918	5
GCCAT	37610	1.2021279	5.4401956	8
ATGAA	66955	1.1933403	8.51257	6
TCTCC	33645	1.1861292	5.6754985	7
CAGTA	49400	1.1790745	28.801838	4
GATGC	36475	1.1769899	13.7404	5
ATCCA	49025	1.159049	5.8465223	6
ATCTC	43815	1.1534568	7.7100763	6
CAGTC	36000	1.1506674	47.034702	4
GTGCC	26515	1.1457821	8.947083	6
GTCGT	31650	1.1372223	6.219896	6
TTTGT	46150	1.1356273	6.9576097	5
GTCGA	35020	1.1300393	8.496116	6
TTATT	61220	1.11428	5.771942	6
GATTT	49825	1.1010756	5.5624304	5
TCGTT	37085	1.0974914	6.156226	7
CTTTA	50130	1.0973305	25.013273	4
TCGCC	25480	1.0906357	7.8663626	7
ATCGG	33660	1.0861543	5.914424	6
GTGTG	29755	1.0793487	45.11026	4
ATGCT	40185	1.0680032	7.448685	6
GTCTC	30005	1.0679114	8.577114	6
TCACT	40220	1.0588161	6.1911974	7
CATCG	33010	1.055098	13.982386	5
GTGGA	32355	1.0540202	6.4606175	6
GTGAA	43635	1.0514276	9.382168	6
GTGGC	24060	1.0496299	6.7691436	6
GTCTT	35430	1.0485134	8.20809	6
TTCTA	47765	1.0455613	5.2327485	6
ATGGA	43375	1.0451627	6.823395	6
CAGTT	39175	1.0411602	15.777766	4
AGTTT	46880	1.0359945	7.181809	3
AAAGT	58095	1.0354283	7.0370283	1
GTTAC	38905	1.0339845	8.326617	5
GTCAT	38545	1.0244167	5.3223305	6
ATCTG	38445	1.021759	6.3146305	6
CATCC	32240	1.0207331	8.782955	5
TCACA	42940	1.0151874	8.093629	7
AGATA	56530	1.0075353	34.888958	4
TCTCA	37915	0.99813557	6.65075	7
GTCTG	27510	0.9884673	5.505566	6
ATCTT	44585	0.9759522	9.170578	6
TTCAC	36970	0.97325796	8.475978	6
TATTT	53405	0.97203726	5.657356	7
TTCAG	36530	0.9708638	7.0363026	6
TCAAT	49195	0.9670888	9.170117	7
AGTCA	40280	0.96139914	15.106095	5
TAAGA	53725	0.95754176	5.963275	7
ATGCC	29900	0.9556932	7.9662447	6
TCGAA	39955	0.9536421	8.911677	7
ATCGA	39755	0.94886863	7.846703	6
GATCT	35540	0.9445523	17.452381	5
CATGA	39545	0.94385624	10.464299	5
TCAGC	29370	0.93875283	8.493415	7
GTCCC	21930	0.9386829	5.1887593	6
TTCAT	42655	0.9337051	5.3282757	6
TAGAA	51845	0.9240344	9.8091545	7
GTTCG	25540	0.9176829	20.283932	5
ATATT	55970	0.91487557	5.7462955	6
TAACA	51675	0.9122875	5.0935745	7
GTTAT	41100	0.9082631	8.799145	5
GTGTA	33780	0.90635496	34.70701	4
ATCCC	28450	0.90074015	5.3117366	6
TGGGA	27440	0.8939055	5.3392305	7
TATTC	40185	0.8796375	6.2943206	7
GTAGA	36465	0.8786595	8.564295	6
GTCAG	27200	0.8777004	8.934221	6
TTTAG	39640	0.87599874	7.513033	5
CCATA	36870	0.8716805	33.861263	4
TTTCG	29420	0.87065387	9.558826	5
TCTCG	24435	0.86966884	6.1266685	7
TTCGT	29305	0.86725056	6.069968	6
TCGAG	26475	0.8543058	5.038343	7
ATGCA	35140	0.8387181	5.2890015	6
GTCGG	19220	0.8384824	6.007615	6
GTGGG	18845	0.8299784	5.5738387	6
CATGC	25795	0.8244851	11.734663	5
TTCGG	22930	0.8239025	5.7146387	6
TTCGA	30825	0.8192411	7.8224096	6
ATCAC	34500	0.8156491	9.102233	6
TTTAA	49300	0.80584896	7.3170476	5
TTCGC	22610	0.80471516	8.784207	6
TGTGC	22340	0.802703	11.762589	5
GTTAA	40440	0.8025771	13.446197	5
GATCC	24940	0.7971568	11.486638	5
GATAT	40145	0.79672235	8.0754175	5
TAAAG	44525	0.79357	5.315093	7
CATGT	29640	0.787747	9.705792	5
TCGAT	29520	0.78455776	5.245144	7
AGTCT	29500	0.78402627	9.241771	5
CGACG	20200	0.78391236	7.6046834	1
TTAGA	39270	0.7793571	8.016113	6
GTAAC	32505	0.7758263	5.323722	6
ATCGT	29030	0.771535	6.263081	6
GATAA	43270	0.771202	13.276999	5
ATCGC	23615	0.7548059	8.927155	6
TAAAA	56835	0.74926263	8.003446	7
AGTCC	23370	0.7469749	6.2006145	5
GTATT	33415	0.73843336	5.5292053	6
TTAAA	50255	0.7377203	7.103726	6
AGTGG	22560	0.7349312	9.116136	5
GTGAT	26850	0.7204154	5.0219135	6
ATCTA	36020	0.7080912	5.1854634	6
TGTAG	26295	0.70552415	9.993724	5
GTTAG	26020	0.69814557	14.613219	5
TCAGG	21365	0.6894143	5.1635194	7
CATAT	34875	0.68558234	6.2542706	5
TGTGT	22675	0.6774547	7.6940484	5
GATAC	28370	0.6771325	7.894527	5
AGTGC	20890	0.67408687	9.62454	5
TCGCA	21000	0.6712226	7.222502	7
ATAAA	50315	0.6633087	7.338433	6
GTAAA	37020	0.65980816	8.555781	6
TAGAG	27265	0.6569766	5.4214673	7
TCGAC	20530	0.65620005	5.1611443	7
AGTCG	20330	0.6560165	10.219226	5
TAGCC	20380	0.6514056	5.3161335	7
CATAC	27245	0.6441262	5.7329955	5
TTAGC	24155	0.6419714	6.4563875	6
AGTGA	26545	0.6396275	8.706189	5
TAGCA	26160	0.62438434	5.867819	7
GTGCA	19030	0.6140676	6.164769	6
TAAAC	34535	0.6096922	5.521605	7
TGTGA	22100	0.5929676	11.229927	5
TCACG	17980	0.57469445	7.5944767	7
TGTAC	21480	0.5708775	5.67138	5
GATAG	23255	0.5603517	13.263792	5
ATAGC	22545	0.53810203	6.53892	6
AGTAA	29860	0.5321953	8.298124	5
GTGAG	16080	0.52383393	5.22852	6
ATAGA	29325	0.5226601	7.389085	6
CATAA	29595	0.5224798	9.555265	5
AGTAG	20765	0.5003528	8.320546	5
TGTAT	22540	0.49810827	5.6803246	5
TGTAA	23960	0.475513	10.260304	5
GTAGC	14565	0.46998924	7.2600336	6
CATAG	17655	0.42138782	9.306744	5
>>END_MODULE