FastQCFastQC Report
Thu 31 Jul 2014
A80_CGATGT_L006_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename A80_CGATGT_L006_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 386824
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTCAGATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 931 0.24067793104874569 No Hit
CGATGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 921 0.23809277604285153 No Hit
TGACCATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 742 0.1918185014373462 No Hit
ACAGTGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 549 0.14192500982358902 No Hit
CGATGTTGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAAT 447 0.11555642876346867 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CAGTG 116000 3.608793 143.40459 2
CGATG 107735 3.3516667 243.53148 1
GACCA 92850 3.3438864 200.3236 2
TCAGA 114165 3.1088393 192.4708 2
TCTTC 144025 3.0243533 8.083573 7
CTCAG 95360 2.9993267 229.81586 1
TGACC 94445 2.970548 171.85858 1
ACCAT 107850 2.969197 154.63335 3
TTCTT 185345 2.9428706 5.9163346 50-54
CAGAT 105215 2.865121 194.19437 3
GCAGC 68545 2.8202276 7.8794107 8
GATGT 119135 2.802457 169.50388 2
CACCA 71420 2.6004171 6.1673083 40-44
ACAGT 94135 2.5634003 130.45032 1
TGTTG 121820 2.5026848 75.091286 4
AGCTT 102000 2.425791 92.4426 2
GATCA 83905 2.284826 116.22873 1
ATCAG 83900 2.28469 116.49133 2
CAACA 71215 2.2449281 5.055074 35-39
TCTTG 106605 2.2142093 6.1829777 7
AGTGT 93885 2.208492 112.645256 3
ATGTT 121010 2.1760561 130.13092 3
TGGAG 67745 2.0846238 5.117205 7
CCATG 66265 2.0842113 86.279724 4
AGATG 76950 2.0726233 89.73939 4
TCAGT 85580 2.035286 102.63138 3
GCGGC 43260 2.0334504 36.75951 8
GGCAG 49490 2.0140595 10.417824 8
TCAGC 61360 1.9299358 6.816372 7
TGGCA 62035 1.9299266 14.721161 7
TTTCT 120310 1.9102579 8.875807 5
CTTTG 90250 1.8745123 43.713856 4
TTGGC 68665 1.8656378 28.40069 6
GCTTT 88200 1.8319336 84.07441 3
TGTTC 87070 1.8084631 45.637222 4
CATCG 57455 1.8071134 15.17291 5
GTTGG 67195 1.8058237 30.801659 5
AGATC 66135 1.8009292 63.939312 4
GATGG 57780 1.7779846 31.344746 5
TAGCT 74230 1.7653577 92.3746 1
CCTTC 63175 1.7544632 5.2645836 9
CCATC 54770 1.7416219 58.02952 4
CGGCA 42150 1.7342271 19.269648 9
TTTGG 81930 1.6831797 14.352903 5
TGGTG 62570 1.6815298 8.273711 4
GGAGG 41755 1.6807758 5.3561435 75-79
ACACC 44485 1.6197083 5.5703588 40-44
AGCGG 39740 1.6172706 8.503943 8
AGTGG 52255 1.6079714 17.828163 5
TGCTG 58820 1.5981477 13.199207 7
TCTGG 58110 1.578857 14.279382 7
TTCTG 75925 1.5769792 8.368168 6
TCCTT 73895 1.551707 5.579091 7
GTTCT 74605 1.5495625 15.819967 5
GCCTT 56045 1.5395095 7.098408 8
ATGGC 49185 1.5301595 32.44387 6
GGTCA 48910 1.5216042 5.590968 60-64
TCTCT 71970 1.5112841 11.616319 7
CTTTC 71450 1.5003648 27.86491 4
CTTTT 94365 1.4983084 5.2962317 4
GTCTT 71940 1.4942098 5.206189 6
CATGG 47405 1.4747832 24.479963 5
GGCGG 31280 1.454321 16.50034 8
TTCTC 68985 1.4486028 12.909284 6
GTGTC 53235 1.4464025 44.484753 4
TGGCG 40350 1.4341227 29.811037 7
TGGCC 39845 1.4317597 39.529293 7
GTTGT 69660 1.4311035 17.15177 5
CAGTC 45360 1.4266932 45.564472 4
GTTCA 59745 1.4208714 5.2347665 5
GCTGG 39510 1.4042673 5.8760486 8
AATAA 66950 1.3816084 5.0601482 20-24
GTTGC 50650 1.3761677 41.507687 5
GTGGC 38635 1.3731679 28.001696 6
ACCGG 33205 1.3661925 5.1664996 8
CGGCG 28930 1.3598641 14.038349 8
AAAGT 56910 1.3417193 10.277786 1
GGCCA 32610 1.3417116 8.457899 8
GTAGC 43125 1.3416312 6.9684186 6
TGGTC 49255 1.3382653 5.4140463 7
GTTGA 56815 1.3364806 6.7858596 5
GATGA 49580 1.3354211 7.0125217 5
TGTCC 48365 1.3285463 23.372805 7
ATCTT 72570 1.3193486 6.637211 6
AGCCT 41695 1.3114191 5.5516253 2
TTGCC 47660 1.3091805 28.63335 6
GATGC 41830 1.3013432 41.810505 5
GTGTG 48380 1.3001823 60.82155 4
TTTCC 61745 1.2965714 10.119738 5
TTGGG 47540 1.2776078 12.247698 6
TTGCT 61040 1.2678144 8.106348 6
CCGGC 26615 1.2648154 16.658167 9
ATGGA 46860 1.262159 5.001483 6
CTCTG 45895 1.2606974 5.739988 8
CATCT 51480 1.2377852 15.713933 5
TTGCA 52020 1.2371534 5.9496465 6
GCGGT 34655 1.2317106 6.5480895 8
CAGTT 51790 1.2316835 8.56703 4
TGCCC 33710 1.2246403 46.8687 7
TGTGG 45530 1.2235904 17.811281 5
ATGGT 51670 1.2154529 14.735009 3
CAGTA 44520 1.2123288 13.202418 4
ATCGG 38780 1.2064569 16.018312 6
TTTGC 57645 1.1972994 19.485443 5
CCCTT 43030 1.1950067 5.22419 9
TTGTC 56340 1.1701941 13.946945 6
GAGAT 43295 1.1661367 5.455077 95-97
CCCAG 27625 1.1491169 6.898503 1
GCCCC 23895 1.1480513 17.027851 8
TGCGG 31845 1.1318372 42.252403 7
GATCT 47570 1.1313223 21.331093 5
TGGCT 41600 1.1302781 13.0016165 7
TGTCT 54125 1.1241883 11.580498 5
ATCTG 47105 1.1202636 10.654017 6
TCTGC 40455 1.111265 8.936268 7
TACCG 35305 1.1104367 7.2890964 7
AGTTT 61340 1.1030434 7.5588574 3
GCCCA 26400 1.0981606 11.414756 8
TGCAG 35295 1.0980375 12.353106 7
GTTTG 53380 1.0966451 5.8069 5
TCCAG 34835 1.0956538 16.072609 9
CCATT 45545 1.0950841 10.922815 4
TGCTT 52670 1.0939676 5.729829 7
GCGGA 26860 1.0931025 10.496747 9
CCATA 39395 1.0845759 17.085081 4
GCTGC 30055 1.0799733 5.01738 8
AGGGG 26820 1.079593 5.2390265 75-79
GCCTC 29600 1.0753294 7.9963894 8
CCGGA 26025 1.0707773 5.645249 9
ATGGG 34330 1.0563899 13.367393 6
GGGCA 25925 1.0550513 8.050137 8
TGTCA 44265 1.052722 6.7567687 7
GGGTC 29480 1.0477803 5.1520386 60-64
CTGGC 29080 1.0449383 6.898897 8
TTTGT 66375 1.0424165 8.657359 5
GATCG 33505 1.04235 17.481377 5
GCCAG 25275 1.0399191 5.465718 8
TGTGC 38250 1.0392579 28.321651 5
TAGCC 32980 1.0373092 6.268537 1
TGCCA 32895 1.0346357 9.378231 7
AGATA 43810 1.0328715 20.163218 4
TCTCC 37035 1.0285167 25.622766 7
TGTTT 65385 1.0268686 9.738577 4
TTGTG 49630 1.0196047 8.565925 6
GTTCC 36995 1.0162219 20.749191 5
CTCCA 31845 1.0126336 16.264914 8
CCCGG 21300 1.0122324 12.626106 8
GGCGA 24860 1.0117098 5.7021804 8
ATGTC 42345 1.00706 16.061739 6
TCGGC 27595 0.9915775 28.536348 7
AGTCC 31445 0.98902917 16.103107 5
TGGGC 27810 0.98842496 27.74322 7
TTAGC 41475 0.98636943 7.1603303 6
GGCCC 20640 0.9808675 21.519678 8
CGGCC 20505 0.97445196 10.759839 9
CCGGT 26820 0.9637294 6.498205 9
CCGCC 20035 0.962595 8.082988 9
GTGTT 46850 0.96249205 8.257153 4
CATGT 40440 0.9617549 14.908709 5
CCTCC 26185 0.96173614 5.146233 9
CCGGG 20455 0.9614941 6.062015 8
TCCGG 26750 0.96121395 21.184492 7
CGCCA 23035 0.9581868 5.364532 9
TGACG 30625 0.95275253 5.414853 7
GTCCC 26195 0.9516302 20.994923 6
GGCCT 26440 0.9500747 10.104445 8
CATCC 29815 0.94808203 18.43871 5
GTGCC 26180 0.9407321 32.891716 6
TGCCG 26150 0.939654 29.111256 7
GGCGT 26435 0.9395547 7.3579845 8
GCCCT 25835 0.93855196 13.386027 8
GATCC 29660 0.9328862 23.361704 5
ATAGC 34010 0.92613 8.027071 6
GTCTG 34000 0.92378485 8.944373 6
AGATT 44855 0.9235742 12.568252 4
CCGTC 25405 0.92293054 5.143052 9
GATAG 34115 0.9188764 8.292274 5
GCCGG 19460 0.91472375 17.889782 8
CGGCT 25370 0.9116261 10.452874 9
GTGCT 33515 0.91060746 9.484462 6
GTGTA 38485 0.90529716 12.020665 4
ATGCC 28780 0.905208 34.49359 6
CGCCC 18795 0.90301836 5.303107 70-74
GATTG 38295 0.9008276 5.998927 5
TCCCC 24450 0.8980121 16.275629 7
CCTCG 24515 0.890598 5.4263225 85-89
GCGCC 18680 0.8877231 13.017792 8
TGCTC 32175 0.8838205 9.708704 7
TTGCG 32320 0.878139 23.368656 6
ATCCT 36520 0.8780869 6.236613 6
GCCTG 24340 0.87461495 6.1497755 8
GTCTC 31760 0.8724207 14.303357 6
TAGCG 28030 0.8720214 12.594436 7
TTTCG 41900 0.8702722 7.3913774 5
GTCCT 31620 0.868575 7.0850887 8
AGTCT 36280 0.86282045 11.2535925 5
TTCGG 31555 0.8573538 12.738157 6
TTCCC 30785 0.854945 16.534292 6
GTGCG 24015 0.85354286 24.744884 6
ATCTC 35320 0.8492341 17.753946 6
CGAGA 23810 0.8481556 6.0283837 95-97
CATGC 26965 0.84812135 37.58917 5
TGTTA 47005 0.845265 15.449015 4
ATGCA 30910 0.84171355 6.5219955 6
CCCGC 17510 0.84127957 10.622024 8
GTCGG 23620 0.8395037 15.439704 6
GGTGC 23560 0.8373712 5.083385 5
TGTGT 40720 0.83655655 10.29903 5
CTTTA 45840 0.8333875 8.849614 4
GTGGG 23530 0.8272011 11.777564 6
CATGA 30305 0.8252387 5.690243 5
TGCCT 29980 0.82352555 18.365295 7
AGTGC 25975 0.8080897 28.220583 5
GCGTG 22505 0.79987437 5.8071213 8
CCCCT 21440 0.7874594 5.6804442 9
GCCGC 16500 0.78412384 10.690719 8
TCCCG 21545 0.78270173 24.640854 7
GATAC 28605 0.7789458 5.1621456 5
CATAG 28580 0.77826506 6.0203032 5
TGCGC 21585 0.7756188 21.881351 7
CCCCG 16040 0.7706525 7.663697 8
TCCTG 28045 0.7703728 5.886484 7
ATGCT 32190 0.7655512 11.253595 6
TGTCG 28135 0.76443195 11.420871 5
GTTCG 28015 0.7611715 14.740432 5
ATGTG 32090 0.75486517 9.9678 6
GTTAC 31635 0.75235194 5.2808876 5
GGGCC 15945 0.7494999 18.208834 8
TCCCT 26960 0.74871904 14.029912 7
TCCCA 23480 0.7466364 6.4597144 7
ATCCG 23470 0.7381943 11.711351 6
ATCCC 22985 0.73089606 20.550837 6
GGCGC 15515 0.7292876 6.4494367 8
GCGCT 20280 0.72872597 6.358832 8
CCCTG 19780 0.7185816 6.6225605 8
TCGCC 19730 0.71676517 11.166762 7
TTCGC 25425 0.6984036 7.69771 6
CGGGC 14855 0.6982641 7.7712293 8
GCCCG 14540 0.6909794 14.008528 8
CCGCA 16045 0.6674238 6.433405 9
GCCGT 18545 0.66638184 6.0975113 8
CCCAC 15780 0.6636245 5.545907 9
TTCCG 24080 0.66145754 10.600999 6
TGCGT 24345 0.6614571 10.511944 7
AGTCG 20860 0.64896053 11.825193 5
TCTCG 23555 0.64703614 7.8175697 7
GTTAG 27450 0.64571667 7.7096486 5
TCCGC 17735 0.6442894 11.360507 7
ATGCG 20465 0.636672 24.962626 6
TGCGA 20350 0.6330943 5.8070154 7
CTCCC 16910 0.62107915 6.517374 8
GTGCA 19630 0.61069494 6.5611734 6
GGCCG 12585 0.59156203 7.6572814 8
TCGCT 19595 0.5382583 6.938593 7
GTCCG 14830 0.53288984 12.264707 6
TACGG 17085 0.53151923 5.1433563 7
CCCGT 14315 0.5200453 5.653835 9
CCCCC 10470 0.5085743 5.3223615 9
ATCGC 16020 0.50387186 10.735406 6
TCGGG 14000 0.497589 11.631474 7
TCGCG 13195 0.474139 9.651487 7
GTCGC 12890 0.46317935 10.243817 6
Produced by FastQC (version 0.10.1)